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All Journal Biotropika JPP Jurnal Kesehatan Poltekkes Palembang
Safitri, Hana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Perbedaan Teknik Pemasangan Tourniquet Terhadap Kadar Magensium Serum Safitri, Hana; Wulan, Wieke Sri; Nidianti, Ersalina
JPP JURNAL KESEHATAN POLTEKKES PALEMBANG Vol 19 No 1 (2024): (JPP) Jurnal Kesehatan Poltekkes Palembang
Publisher : Poltekkes Kemenkes Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36086/jpp.v19i1.2144

Abstract

Background: Magnesium is an electrolyte that plays an important role in the body's reactions, because if there is a lack or too much magnesium it will cause organ dysfunction and even a person's death. That is the reason why errors in magnesium measurements can create serious conditions for patients if laboratory results are inaccurate. The hemoconcentration state of the sample can influence the final results obtained. The aim of the study was to determine the difference between tourniquet application techniques and the results of serum magnesium levels. Methods: This type of research is experimental research. In the first group of treatments, a tourniquet was dabbed which was maintained only until the vein could be accessed and the second group was dabbed with a tourniquet until the blood drawn reached the desired volume at the research object, measured and collected at the same time. Then the serum magnesium test was measured. Results:Research results on differences in tourniquet application techniques in serum magnesium examination. This research involved 20 respondents with a total sample of 40 samples (respondents' blood was taken twice). The research results mean magnesium levels with the released tourniquet technique were 2.8 mg/dL, higher than the second intervention, namely the tourniquet technique which was maintained at 2.5 mg/dL. The statistical results of the paired T-test carried out obtained data with a p-value of 0.849, which means p-value > 0.05. Conclusion: There was no significant difference between magnesium levels with the tourniquet removed and the tourniquet maintained to a volume of 3 cc.
Secondary Metabolite Profile in Stem and Root of Keji Plant (Staurogyne elongata [Blume] Kuntze) Safitri, Hana; Malik, Abdul; Mukaromah, Arnia Sari
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.02

Abstract

Keji (Staurogyne elongata [Blume] Kuntze) is an herbal medicinal plant that is often found on the Java to Sumatra islands. The leaves and roots of keji (S. elongata) have been used by the community as diuretic drugs, increasing blood pressure and trusted to treat bladder stones, kidney stones, and joint problems. However, research on secondary metabolites in the stems and roots of keji has never been done. The study aimed to identify the secondary metabolite content and marker compounds in the stems and roots of S. elongata. The research stages were drying and sample grinding, extraction, and metabolite compound analysis using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed nine secondary metabolite compounds found in S. elongata stem, such as phenol, terpenoid, and alcohol. Meanwhile, secondary metabolite compounds of S. elongata root were ten compounds from the coumaran, phenols, and ester group. The 2,2’-methylenebis[6-(1,1-dimethylethyl)-4-methyl-phenol is proposed as a marker compound in S. elongata stem. Meanwhile, no secondary metabolites can be used as marker compounds in S. elongata roots because octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester is the primary metabolite.
Co-Authors Abdul Malik Arnia Sari Mukaromah Ersalina Nidianti Wulan, Wieke Sri