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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science)
Mayashinta, Dearikha Karina
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Agriculture, Biological Sciences & Forestry Environmental Science Other

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GENOTYPING OF TOXOPLASMA GONDII IN CEREBRAL AND OCULAR TOXOPLASMOSIS Mayashinta, Dearikha Karina; Halleyantoro, Ryan; Sari, Ika Puspa; Kurniawan, Agnes
Journal of Tropical Life Science Vol 8, No 3 (2018)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.022 KB) | DOI: 10.11594/jtls.08.03.02

Abstract

Toxoplasma gondii is an obligate intracellular protozoon, spread world-wide and capable of infecting birds and mammals. Genetic information on T. gondii type that causes human toxoplasmosis is limited. In this study, genetic analysis of SAG2 locus was used to determine the genotype of T. gondii from cases with cerebral and ocular toxoplasmosis in Indonesia. Genotype determination was done directly on the clinical samples. A number of 28 cerebrospinal fluid and 8 vitreous humor positively infected with T. gondii, underwent PCR-RFLP to classify each isolate into one of three genotypes of T. gondii. Type I was the most common found suggesting that cerebral and ocular toxoplasmosis in Indonesia is mostly caused by type I strain of T. gondii. 
Risk factors of toxoplasmosis in goat and sheep that influence in human infection Fatmawati, Mira; Padaga, Masdiana C; Mayashinta, Dearikha Karina; Aini, Fidi Nur
Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) Vol 31, No 1 (2021): April 2021
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2021.031.01.04

Abstract

Toxoplasmosis is a zoonosis that is transmitted from animals to humans. The definitive landlady of toxoplasmosis is a cat. However, goats can be infected by this disease. One third of the world's humans are estimated to be infected with toxoplasmosis. The purpose of this study was to determine the factors that influence the incidence of toxoplasmosis in humans and animals. The research method is by serological testing of toxoplasmosis in humans using ELISA and serological testing of toxoplasmosis in goats and sheep using ToMAT. The results showed that the prevalence of toxoplasmosis in humans was 15.1% while in goats it was 61.1% and in sheep it was 58.9%. Risk factors that influence the incidence of toxoplasmosis are maintenance management factors such as feeding from grass that take it from fields, provision of drinking water collected, procedures for providing cages, and also cat litter available. While health management factors that become risk factors are separated of sick animals. Potential human transmission through knowledge, attitudes and practices of farmers related to the control of toxoplasmosis. The conclusion of this study is the need for socialization to farmers to improve knowledge, attitudes and practices of farmers related to maintenance management and health enforcement factors that are a risk of transmitting toxoplasmosis, enclosure sanitation and maintenance management.
Co-Authors Agnes Kurniawan Dameanti, Fidi Nur Aini Eka Puji Fatmawati, Mira Halleyantoro, Ryan Ika Puspa Sari Padaga, Masdiana C