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SKRINING KAPANG Aspergillus spp. PENGHASIL AFLATOKSIN PADA JAGUNG PIPILAN DI DAERAH BEKASI, JAWA BARAT Sukmawati, Dalia; Wahyudi, Priyo; Rahayu, Sri; Moersilah, Moersilah; Handayani, Tri; Rustam, K. Yoswita; Puspitasari, Sherly Indah
Al-Kauniyah: Jurnal Biologi Vol 11, No 2 (2018): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

AbstrakAflatoksin merupakan senyawa metabolit sekunder dari kapang Aspergillus flavus dan Aspergillus parasiticus yang dapat mengontaminasi bahan pangan atau pakan sehingga berbahaya bagi kesehatan hewan dan manusia. Kontaminasi kapang penghasil aflatoksin banyak ditemukan pada bahan pangan dan pakan yang berasal dari produk pertanian. Jagung merupakan salah satu produk pertanian yang mudah terkontaminasi oleh kapang penghasil aflatoksin. Penelitian ini bertujuan untuk memperoleh isolat kapang Aspergillus spp. penghasil aflatoksin pada jagung pipilan yang dijual di sekitar Bekasi, Jawa Barat. Isolasi kapang dilakukan menggunakan metode dilution plating pada medium Dichloran-Glycerol. Hasil penelitian memperoleh 12 isolat kapang, dengan warna koloni hijau (J1, J2, J3, J4, J5, J6, J7, J9, J10, J12), hitam (J11), dan jingga (J8). Identifikasi dilakukan dengan cara mengamati morfologi kapang secara makroskopik dan mikroskopik pada medium Malt Extract Agar. Isolat kapang yang diduga memiliki kemiripan dengan A. flavus berjumlah 6 isolat, yaitu J1, J2, J4, J6, J10, dan J12. Selanjutnya dilakukan uji konfirmasi menggunakan medium selektif Aspergillus flavus dan parasiticus Agar. Terdapat 2 isolat kapang, yaitu J1 dan J4, yang menunjukkan pigmentasi sebalik koloni berwarna pada medium selektif AFPA. Isolat kapang yang ditemukan pada jagung pipilan diharapkan dapat memberikan informasi kepada petani dan peternak mengenai jenis kapang yang dapat menyebabkan kontaminasi pada jagung, sehingga mereka dapat menjaga dan meningkatkan kualitas jagung untuk mengurangi kerugian dalam bidang ekonomi dan kesehatan.Abstract Aflatoxin is a secondary metabolite secreted by the mold Aspergillus flavus and Aspergillus parasiticus that may contaminate food or feed so harmful to human and animal health. Contamination of aflatoxin-producing mold is commonly found in food and feed which derived from agricultural products. Corn is one of the agricultural products that are easily contaminated by aflatoxin-producing mold. The study aims to isolate the aflatoxin-producing mold Aspergillus spp. in stripped corn vend around Bekasi, West Java. The isolation was conducted by using the method of dilution plating on Dichloran-Glycerol medium. The study obtained 12 isolates of mold, with green colony color (J1, J2, J3, J4, J5, J6, J7, J9, J10, J12), black (J11), and jingga (J8). Identification was conducted by observing the morphology of mold on Malt Extract Agar macroscopically and microscopically. The isolates that allegedly have similarities to A. flavus are J1, J2, J4, J6, J10, and J12. Furthermore, a confirmatory test was preceed by using a selective medium of Aspergillus flavus and parasiticus agar. There are 2 isolates of molds, J1 and J4, which showed yellowish jingga pigmentation like the positive control of A. flavus. The isolates of mold found in the stripped corn may provide information to farmers and breeders about the type of mold that can cause contamination in corn, so that they can anticipate in advance and improve the quality of the corn to reduce losses in economic and health perspectives.

Analisis Miskonsepsi pada Materi Larutan Penyangga Menggunakan Two-Tier Diagnostic Test Stephanie, Mian Maria; Fitriyani, Dewi; Paristiowati, Maria; Moersilah, Moersilah; Yusmaniar, Yusmaniar; Rahmawati, Yuli
Jurnal Riset Pendidikan Kimia (JRPK) Vol. 9 No. 2 (2019): Jurnal Riset Pendidikan Kimia (JRPK), Volume 9 Nomor 2 (2019)
Publisher : LPPM Universitas Negeri Jakarta

This study aims to find out the misconceptions that occur in buffer solution materials using two-tier diagnostic tests instrument. The instrument was developed based on the buffer solution material competency indicators into 10 questions and was declared valid for use. The research was conducted on level I and III college students and students totaling 159. The results showed that there was a misconception on the concept of buffer solution properties of 24.6%, the concept of buffer solution composition was 24.1%, the working principle of buffer solution was 29.3 %, and the concept of pH solution is 25.4%. he cause of the misconception is because the initial concept of the respondent is weak, the teacher's explanation often simplifies the concept, the habit of the respondent to memorize, understanding the language and weak mathematical concepts, and learning models that have not provided the opportunity for respondents to understand the components and principles of working buffer solutions microscopically. Knowledge of misconceptions is needed as an evaluation of teachers and lecturers to be more motivated to implement learning models that are able to overcome misconceptions to prevent wider misconceptions in learning chemistry that make chemistry difficult. Keywords Misconception, Two-Tier Diagnostic Test, Buffer Solution

The Influence of Green Chemistry Activities on Students’ Environmental Care Attitudes Anggraeni, Denisa Ajeng Retno; Moersilah, Moersilah
Journal of Education for Sustainable Development Studies Vol. 1 No. 1 (2024): Journal of Education for Sustainable Development Studies
Publisher : Scientia Publica Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70232/b8rkjq30

This study aims to investigate the effect of green chemistry activities on students’ environmental care attitudes. A quasi-experimental with a pretest-posttest control group design was used in this study. The participants were 44 eleventh-grade students at a public school in West Java, Indonesia. Two intact classes were randomly assigned to the control group (n=19) and the experimental group (n=25). Control group students carried out traditional experiments, while experimental group students carried out green chemistry experiments. The data obtained were analyzed using independent samples t-test and paired samples t-test. The results of the independent samples t-test (ɑ=5%) show that the p values obtained for the pretest and posttest scores are 0.189 and 0.037, respectively. In addition, the results of the paired samples t-test indicated that the p-values for the control and experimental groups were 0.349 and 0.000, respectively. This shows that there is a significant difference in the environmental care attitude score between the two groups in favor of the experimental group. The experimental group also showed a greater increase in scores than the control class after treatment. It can be concluded that green chemistry experiments and activities are effective in promoting the environmental care attitudes of eleventh-grade students on the topic of acids and bases. It is suggested to teachers use the green chemistry curriculum on other topics to improve student learning.

Development of Learning Cycle 5E Practical Instructions on Acid-Base Titration Using Natural pH Indicators Based on Green Chemistry Jani, Apriliany Kartika; Moersilah, Moersilah
Journal of Computers for Science and Mathematics Learning Vol. 1 No. 2 (2024): Journal of Computer for Science and Mathematics Learning
Publisher : Scientia Publica Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70232/4eagx078

This research aims to produce a learning media product in the form of a practical guide for the 5E learning cycle on acid-base titration material using natural pH indicators based on green chemistry that is valid and practical. The type of research used is Research and Development (R&D) with the Borg and Gall model using a descriptive quantitative method. The study was conducted from November 2021 to August 2022 at SMA N 53 Jakarta with 91 student respondents, eight teachers, three subject matter and language experts, and three media experts. The practical guide is equipped with a barcode to facilitate students’ access to videos, additional materials, and evaluation questions. The research results show that the pH range of the Red Shoot leaf extract is 2-5 with a color change from pink to yellow and 6-14 with a color change from yellow to brick red. The feasibility test results of the practical guide by the experts showed an average percentage of 85% for subject matter and language experts and 88% for media experts. The reliability test from the experts was categorized as “Very Reliable” with a value of 0.93 for both subject matter and language experts and media experts. The student trial results showed an average of 80% on a small scale and 90% on a large scale. The teacher trial results showed an average of 95% on a small scale. The trial results conclude that the practical guide for the 5E learning cycle on acid-base titration material using natural pH indicators based on green chemistry has met the criteria of “Very Good” and is suitable as a learning media. The attractive, innovative, and creative acid-base titration learning can use this practical guide as learning media.

Analysis of Students' Science Process Skills in Project-Based Chemistry Learning Integrated with Education for Sustainable Development Paristiowati, Maria; Moersilah, Moersilah; Stephanie, Mian Maria
LAVOISIER: Chemistry Education Journal Vol 2, No 2 (2023): LAVOISIER: Chemistry Education Journal
Publisher : UIN Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/lavoisier.v2i2.8758

This study aims to analyze the profile of science process skills through the implementations of project-based learning integrated education for sustainable development on acid-base material. The aim of the project is to create a natural acid-base indicator that can determine the pH range of various solutions and the concentration of a solution using the titration method. This study uses a qualitative research method, the interpretive paradigm with the research subjects of XI IPA students in Jakarta totaling 29 people. Data collection techniques are carried out by class observation, reflective journals of student, interviews, and tests of science process skills. The results showed that science process skills arises during learning and were classified as very good with an average value of 86.99% according to observations and classified as good with an average value of 72.26% in the test of science process skills. Students apply the education for sustainable development (ESD) concept during the project by choosing safe solvents and conducting small-scale experiments to reduce the waste produced. The application of this project is expected to make students have the skills of a researcher by paying attention to sustainable development in every decision they make during learning.

Analysis of Students' Science Process Skills in Project-Based Chemistry Learning Integrated with Education for Sustainable Development Paristiowati, Maria; Moersilah, Moersilah; Stephanie, Mian Maria
LAVOISIER: Chemistry Education Journal Vol 2, No 2 (2023): LAVOISIER: Chemistry Education Journal
Publisher : UIN Syekh Ali Hasan Ahmad Addary Padangsidimpuan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24952/lavoisier.v2i2.8758

This study aims to analyze the profile of science process skills through the implementations of project-based learning integrated education for sustainable development on acid-base material. The aim of the project is to create a natural acid-base indicator that can determine the pH range of various solutions and the concentration of a solution using the titration method. This study uses a qualitative research method, the interpretive paradigm with the research subjects of XI IPA students in Jakarta totaling 29 people. Data collection techniques are carried out by class observation, reflective journals of student, interviews, and tests of science process skills. The results showed that science process skills arises during learning and were classified as very good with an average value of 86.99% according to observations and classified as good with an average value of 72.26% in the test of science process skills. Students apply the education for sustainable development (ESD) concept during the project by choosing safe solvents and conducting small-scale experiments to reduce the waste produced. The application of this project is expected to make students have the skills of a researcher by paying attention to sustainable development in every decision they make during learning.

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