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All Journal Indobiosains Journal of Innovation in Community Empowerment (JICE)
Marmaini, Marmaini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences

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Journal : Indobiosains

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Keberadaan Escherichia coli Pada Air Sungai Musi Kota Palembang Masito, Masito; Novianti, Dewi; Marmaini, Marmaini
Indobiosains 2024: Volume 6 No 2 Agustus 2024
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v6i2.15895

Abstract

This research is entitled The Existence of Escherichia coli in Musi River Water, Palembang City. This research was conducted from April to May 2024 at the Palembang Public Health Laboratory Center. This research aims to analyze the number of E.coli in the Musi River water in the city of Palembang. The location for sampling river water is divided into 3 (three) stations, namely upstream (Musi VI), middle (Ampera) and downstream (Musi IV).  Each river water sample was taken 2 (two) times on different days at low tide. This sample was taken by purposive sampling and 10 ml of each river water sample was taken. River water samples were tested using the membrane filter method. The results showed that the ALT value in river water at station 1 on the 1st day was 2.2 x 103 CFU/ml, at station 1 on the 2nd day as much as 2.4 x 103 CFU/ml, at station 2 on the 1st day as much as 1.9 x 103 CFU/ml, at station 2 on the 2nd day as much as 2.3 x 103 CFU/ml, at station 3 on the 1st day as much as 1.7 x 103 CFU/ml, at station 3 on the 3rd day as much as 2.1 x 103 CFU/ml. All positive samples contained E.coli bacteria, and exceeded the threshold for hygienic water and sanitation, namely above 0 CFU/100 ml (Permenkes, 2023)  
Pembuatan Virgin Coconut Oil (VCO) Dengan Cara Penambahan Ragi Tempe Razelita; Marmaini, Marmaini; Arif Setiawan, Andi
Indobiosains 2024: Volume 6 No 2 Agustus 2024
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v6i2.15911

Abstract

  Virgin Coconut Oil (VCO) is an innovative coconut oil that is processed without using heating or chemicals . The aim of this research is to analyze the quality of VCO by adding tempeh yeast and fermentation time to the VCO obtained. The research was carried out in March-May 2024 and quality testing was carried out at the Biology Laboratory, Faculty of Science and Technology, PGRI University, Palembang. The research method was a survey and experiment, the survey was carried out on community plantations in Kumbang Ilir Village, Kandis District, Ogan Ilir Regency. The experimental method was carried out with 16 treatments and 3 repetitions, then the data was analyzed using a Completely Randomized Design (CRD) with 2 factors, factor 1 was a variation of adding control tempe yeast starter (without yeast), 15 ml, 30 ml, 45 ml, and factor 2 was a variation fermentation time 6, 12, 18, 24 hours. The research results showed that the highest value for the amount of VCO was 104 ml in the T 4 S 3 treatment, the lowest value was in the T 1 S 0 = 15 ml treatment. The lowest VCO yield value was in the T 1 S 0 treatment = 7.5%, while the highest VCO yield value was in the T 4 S 3 treatment = 52%. The results of the organoleptic test assessed by the respondents stated that 100% of the VCO obtained was clear in color, in the taste test 80% of respondents assessed the taste as typical of coconut and 20% had no taste, and in the aroma test 64% assessed the aroma as typical of coconut, 18% assessed it as slightly rancid, and 18% rated rancid.
Keberadaan Bakteri Salmonella sp Pada Telur Ayam Di Pasar Tradisional Kota Palembang Sumatera Selatan Aryani, Aryani; Marmaini, Marmaini; Novianti, Dewi
Indobiosains 2025: Volume 7 Nomor 1 Februari 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i1.15887

Abstract

Eggs are a product of animal origin that is famous as a high source of protein compared to other protein source ingredients. Damage that can cause a decrease in egg quality is caused by microorganisms, one of which, bacteria that can contaminate eggs is Salmonella sp. This study aims to analyze the presence of Salmonella sp bacteria in chicken eggs sold in several traditional markets in Palembang City. This study used 6 samples of chicken eggs from 3 traditional markets with a simple random sampling method. The test results of all positive egg samples contained Salmonella sp. This shows that the quality of eggs sold at the Palembang Traditional Market does not meet the requirements of the Indonesian National Standard, which is negative/25 grams.     Keywords: Salmonella sp, chicken egg, traditional market.
Uji Efektivitas Ekoenzim Kulit buah Naga ( Hylocereus polyrhizus ) Dalam Menekan pertumbuhan Bakteri Staphylococcus aureus Dwi Ambarwati, Dinda; Marmaini, Marmaini
Indobiosains 2025: Volume 7 Nomor 2 Agustus 2025
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/indobiosains.v7i2.19738

Abstract

  The study entitled The Effectiveness of Dragon Fruit Peel Ecoenzyme in Suppressing the Growth of Staphylococcus aureus Bacteria was conducted on May 8-9, 2025, at the Center for Public Health Laboratory of South Sumatra Province. The method used in the antibacterial test was the disc diffusion method. This study aimed to evaluate the effectiveness of ecoenzyme from dragon fruit peel (Hylocereus spp.) in suppressing the growth of Staphylococcus aureus bacteria, the cause of infection in humans. Ecoenzyme was made with the addition of EM4 to accelerate the fermentation process. Antibacterial activity tests were carried out using the disc diffusion method on NA agar media. The test results showed that dragon fruit peel ecoenzyme did not form an inhibition zone against the growth of Staphylococcus aureus at various concentrations tested. This finding indicates that the ecoenzyme does not have significant antibacterial ability against this gram-positive bacteria. This may be due to the low content of active antimicrobial compounds in the ecoenzyme, or because of the natural resistance of Staphylococcus aureus to the compounds contained in the ecoenzyme.  
Co-Authors Andi Arif Setiawan, Andi Aryani Aryani, Aryani Dewi Novianti Dewi Rosanti, Dewi Dian Mutiara Dwi Ambarwati, Dinda Dwi Warsari, Dwi Masito, Masito Razelita Rizki Wahyudi Trimin Kartika, Trimin