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All Journal PREPOTIF : Jurnal Kesehatan Masyarakat Jurnal Farmasi dan Sains Indonesia (JFSI) Jurnal Teknik Kimia USU
Pramukantoro, Ganet Eko
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health

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UJI AKTIVITAS FIBRINOLITIK EKSTRAK ETIL ASETAT BAKTERI YANG BERASAL DARI LIMBAH CAIR RUMAH POTONG AYAM (RPA) DI KARANGANYAR Ardina, Duanty Indi; Indrayati, Ana; Pramukantoro, Ganet Eko
PREPOTIF : JURNAL KESEHATAN MASYARAKAT Vol. 8 No. 2 (2024): AGUSTUS 2024
Publisher : Universitas Pahlawan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/prepotif.v8i2.24634

Abstract

Atherothrombosis adalah penyakit yang terjadi akibat tersumbatnya bekuan darah (trombus) pada pembuluh darah (arteri). Salah satu pengobatan untuk aterotrombosis dapat berupa fibrinolitik. Agen fibrinolitik dapat berasal dari hewan, tumbuhan atau mikroba. Tujuannya untuk mengetahui ekstrak metabolit sekunder bakteri limbah cair RPA yang mempunyai aktivitas fibrinolitik. Tiga isolat bakteri berhasil diisolasi dari limbah cair RPA. Hasil identifikasi bakteri menunjukkan bahwa bakteri limbah cair RPA merupakan bakteri gram positif pada koloni A dan B, bakteri gram negatif pada koloni C. Hasil uji SIM, sitrat, katalase, dan koagulase menunjukkan 2 dari 3 koloni menunjukkan hasil positif. . Ekstrak tersebut disaring fitokimianya dan hasilnya menunjukkan 3 koloni positif mengandung flavonoid. Konsentrasi yang paling efektif dalam melisiskan bekuan darah adalah konsentrasi 80% dengan persentase 55,04% pada koloni A.
UJI AKTIVITAS ANTIOKSIDAN KOMBINASI EKSTRAK ETANOL DAUN TEH HIJAU (Camellia Sinensis L.) DENGAN EKSTRAK ETANOL KULIT BUAH NAGA MERAH (Hylocereus polyrhizus) MENGGUNAKAN METODE DPPH Illah, Galuh Sekar Rahma; Rejeki, Endang Sri; Pramukantoro, Ganet Eko
Jurnal Farmasi & Sains Indonesia Vol 7 No 2 (2024)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol7no2p178-185

Abstract

Red dragon plant (Hylocereus polyrhizus) and green tea (Camellia Sinensis L.) are plants that have benefits as antioxidants. Antioxidant compounds are able to reduce free radicals so that they become harmless substances. The purpose of this study was to determine the potential antioxidant activity of the combination of green tea leaves and red dragon fruit peels by making three comparisons, namely 1:1, 1:2, 2:1. Extraction was carried out by maceration using 96% ethanol solvent to obtain a thick extract. The extract was subjected to phytochemical screening, then the viscous extract was made into 3 combination variations, namely 1:1, 1:2, and 2:1. In testing the antioxidant activity using the DPPH method spectrophotometrically. The results of data on the percentage of antioxidant activity were tatistically analyzed using SPSS with the one way ANOVA method. The research results show that the combination of green tea leaves and red dragon skin has strong antioxidant activity. The IC50 value at a 1:1 ratio is 13.25 ppm, at a 1:2 ratio is 12.15 ppm, and at a 2:1 ratio is 11.40 ppm. The 2:1 ratio is the strongest combination.
Uji Aktivitas Enzim Fibrinolitik Dari Makanan Fermentasi Kacang Koro (Mucuna Pruriens) Metode Clot Lysis In Vitro Pamungkas, Bayu Aji; Indrayati, Ana; Pramukantoro, Ganet Eko
Jurnal Farmasi & Sains Indonesia Vol 7 No 2 (2024)
Publisher : LPPM Sekolah Tinggi Ilmu Farmasi Nusaputera

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52216/jfsi.vol7no2p153-159

Abstract

Fibrinolytic enzymes are a group of serine proteases that can destroy blood clots (fibrin) in various thrombosis diseases. This study aims to determine the activity of fibrinolytic enzymes contained in jack bean tempeh using the in vitro clot lysis method. First, crude extraction of the jack bean tempeh enzyme was carried out by centrifugation to produce pellets and supernatants. Then purification using 70% ammonium sulfate salt, determination of enzyme protein levels using the Lowry method and fibrinolytic potential testing using the in vitro clot lysis method. The variations in extract concentrations used in the test were 12.5; 25; 50 and 100% with positive control Natokinase and negative control aquadest. This study showed the results of pellets after purification as much as 26.78 gr. Then it also showed an increase in protein level activity between before purification 22,515 µg / mL and after purification 27,215 µg / mL. In vitro clot lysis testing was carried out with several variations showing the most optimal variation in destroying blood clots was at 100% variation after purification with a lysis percentage of 61%. The most optimal results were also in the Two Way Anova test with a value of 63,592 samples after 100% concentration purification.
Kuantifikasi Flavonoid dan Analisis Aktivitas Antioksidan Ekstrak Daun Nilam (Pogostemon cablin) Menggunakan Uji DPPH Anggraini, Natasya Dila Putri; Pramukantoro, Ganet Eko; Novita, Mega; Marlina, Dian
Jurnal Teknik Kimia USU Vol. 14 No. 2 (2025): Jurnal Teknik Kimia USU
Publisher : Talenta Publisher (Universitas Sumatera Utara)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32734/jtk.v14i2.20795

Abstract

Patchouli leaves (Pogostemon cablin) are known to contain flavonoid compounds with potential antioxidant properties. However, comparative data on flavonoid content and antioxidant activity across different extract fractions of patchouli leaves remain limited. To address this gap, the present study was conducted to analyze the total flavonoid content and evaluate the antioxidant activity of the ethanol extract and three solvent fractions—n-hexane, ethyl acetate, and wáter using the DPPH method as an indicator of free radical scavenging activity. The extraction was carried out through maceration, followed by fractionation. The determination of flavonoid content employed a colorimetric method based on the aluminum chloride reaction, while antioxidant activity was assessed using the DPPH assay. The results revealed that the ethyl acetate fraction contained the highest flavonoid concentration (1.615 ± 0.0591%) and exhibited very strong antioxidant activity, with an IC₅₀ value of 17.3909 ± 0.1327 μg/mL. These findings suggest that the ethyl acetate fraction holds significant potential as a natural antioxidant agent, with possible applications in the pharmaceutical field or nutraceutical-based health products.
Co-Authors Ana Indrayati Anggraini, Natasya Dila Putri Ardina, Duanty Indi Endang Sri Rejeki Illah, Galuh Sekar Rahma Marlina, Dian Mega Novita Pamungkas, Bayu Aji