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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Farmasi Jurnal Farmasi Udayana Microbiology Indonesia Indonesian Journal of Biotechnology WARTA JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Jurnal Farmasi Indonesia Jurnal Mandala Pharmacon Indonesia Journal Syifa Sciences and Clinical Research (JSSCR) Jurnal Kefarmasian AKFARINDO PREPOTIF : Jurnal Kesehatan Masyarakat International Journal of Clinical Inventions and Medical Sciences (IJCIMS) Lumbung Farmasi : Jurnal Ilmu Kefarmasian Infokes : Jurnal Ilmiah Rekam Medis dan Informasi Kesehatan Jurnal Insan Farmasi Indonesia Jurnal Farmasi dan Sains Indonesia (JFSI) Jurnal Buana Farma Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Multidisiplin Madani (MUDIMA) Medical Sains : Jurnal Ilmiah Kefarmasian EduNaturalia: Jurnal Biologi dan Kependidikan Biologi Formosa Journal of Multidisciplinary Research (FJMR) Eduvest - Journal of Universal Studies Journal of Health Management and Pharmacy Exploration (JOHMPE) Borneo Journal of Pharmacy Medical Sains : Jurnal Ilmiah Kefarmasian Jurnal Sains dan Kesehatan

Ana Indrayati

Fakultas Farmasi Universitas Setia Budi Surakarta

Author-ID : 226824

Aerospace Engineering Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Identification of Thymocyte Subset by Multicolor Flow Cytometry ED LSR II FACSDriva Ã¢â¬â FlowJo Software Analysis H, Harapan; W, Wienands; I, Ichsan; Indrayati, Ana
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.242 KB)

In their development, thymocytes express different cell surface molecules that important for identification of thymocyte subset. It&rsquo;s not easy to detect this cell surface molecules to determine the thymocyte subpopulation for research. Here we used multicolor flow cytometry ED LSR II FACSDriva - FlowJo software to identify of thymocyte subset from thymocyte sample solution using several antibodies such as mouse anti rat CD2-FITC, mouse anti rat CD45RC-PE, mouse anti rat CD4-APC, mouse anti rat CD8&aacute;-PerCP, mouse anti rat CD3-Biotin + PE-Cy7 or APC-Cy7. We determined double negative and single positive thymocyt subset (CD4 or CD8), found that the double negative thymocyte subset express CD2 and CD45RC. It was useful to determine the thymocyte subset using multicolor flow sitometry ED LSR II FACSDriva - FlowJo software.

Identification of Thymocyte Subset by Multicolor Flow Cytometry ED LSR II FACSDriva - FlowJo Software Analysis H. Harapan; W. Wienands; I. Ichsan; Ana Indrayati
Indonesian Journal of Biotechnology Vol 13, No 1 (2008)
Publisher : Universitas Gadjah Mada

In their development, thymocytes express different cell surface molecules that important for identification of thymocyte subset. It’s not easy to detect this cell surface molecules to determine the thymocyte subpopulation for research. Here we used multicolor flow cytometry ED LSR II FACSDriva - FlowJo software to identify of thymocyte subset from thymocyte sample solution using several antibodies such as mouse anti rat CD2-FITC, mouse anti rat CD45RC-PE, mouse anti rat CD4-APC, mouse anti rat CD8á-PerCP, mouse anti rat CD3-Biotin + PE-Cy7 or APC-Cy7. We determined double negative and single positive thymocyt subset (CD4 or CD8), found that the double negative thymocyte subset express CD2 and CD45RC. It was useful to determine the thymocyte subset using multicolor flow sitometry ED LSR II FACSDriva - FlowJo software.

Pemanfaatan Tanaman Obat Keluarga (TOGA) sebagai Alternatif Pengobatan Mandiri Ismi Puspitasari; Ghani Nurfiana Fadma Sari; Ana Indrayati
WARTA LPM WARTA LPM, Vol. 24, No. 3, Juli 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/warta.v24i3.11111

TOGA (Tanaman Obat Keluarga) merupakan jenis tanaman pilihan yang berkhasiat sebagai obat dengan perawatan yang mudah dan biaya relatif murah. TOGA menjadi alternatif obat keluarga yang aman karena jarang menimbulkan efek samping, mudah diolah dan dikonsumsi untuk pertolongan pertama pada kasus penyakit ringan seperti demam, batuk, atau membantu menjaga stamina. Keberadaan TOGA di lingkungan rumah menjadi sangat penting. Tujuan dari pengabdian ini yaitu memberikan penyuluhan mengenai pemanfaatan TOGA sebagai alternatif pengobatan mandiri dan untuk memberikan informasi mengenai penyakit artritis yang dapat diobati menggunakan TOGA kepada masyarakat RW 21 Kelurahan Nusukan. Metode yang digunakan dalam pengabdian ini adalah dengan memberikan penyuluhan, pelatihan, serta pemberian bibit pohon TOGA untuk ditanam. Target mitra yaitu kelompok ibu-ibu rumah tangga di Wilayah RW 21 Kelurahan Nusukan, Banjarsari, Surakarta. Hasil pengembangan kegiatan di RW 21 dapat meningkatkan motivasi ibu-ibu rumah tangga yang datang untuk lebih memanfaatkan TOGA sebagai pengobatan, meningkatkan pengetahuan masyarakat mengenai artritis dan bahanbahan alamiah yang dapat digunakan untuk mengurangi nyeri artritis.

16S rDNA-Based Identification of Novel Superoxide Dismutase Producing Bacteria Isolated from Indonesia ANA INDRAYATI; VALENTINA YURINA; LARAS AJENG PITAYU; DEBBIE SOEFIE RETNONINGRUM
Microbiology Indonesia Vol. 5 No. 2 (2011): June 2011
Publisher : Indonesian Society for microbiology

Superoxide dismutase (SOD) has therapeutic importance because of its antioxidant activity and protects cells from reactive oxygen species attack. This research was intended to screen bacteria isolated from Indonesia for producing novel SODs and to identify the producers using 16S rDNA approach. Intracellular proteins were each extracted and assayed for their inhibition reduction activity by colorimetric method and by zymography for the presence of SOD protein band(s). For species identification, each of 16S rDNAgenes was amplified by polymerase chain reaction from genomicDNAfollowed by sequencing, BLAST, multiple alignment and phylogenetic analyses. All 16 intracellular proteins gave inhibition reduction percentage in the range of 15 to 70% and in zymography, their SOD profiles were quite diversed with at least one intenseSOD band present in most isolates. The SOD producers were assigned to three species, Flavobacterium okeanokoites, Escherichia fergunosii, and E. coli, and to four genera, Pantoea, Escherichia, Bacillus, and Pectobacterium. The remaining five were grouped in gamma-proteobacterium cluster and two formed a cluster with Pseudomonas. Three marine and four soil isolates could be attractive candidates for novel SODs based on unique properties of SOD producers. In conclusion, 16s rDNA-based identification of bacteria isolated from Indonesia reveals that seven isolates might be attractive candidates for novel SOD producers to be applied in pharmaceutical fields in the future.

Pengaruh Superoksida Dismutase Rekombinan Staphylococcus equorum Terhadap Viabilitas Sel dan Deposisi Kolagen Pada Sel Fibroblas 3T3 Yang Dipaparkan UVA Ana Indrayati; Sukmadaja Asyarie; Tri Suciati; Debbie Soefie Retnoningrum
Jurnal Farmasi Indonesia Vol 13 No 1 (2016): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

UVA is the main external factors causing skin aging. UVA increase matrix metalloproteinase (MMP) synthesis that degrade collagen indirectly through reactive oxygen species. Superoksida dismutase (SOD) catalyzes the conversion of superoxide anions to hydrogen peroxide and oxygen. SOD can be used as a cosmeticâ€™s component to prevent skin aging. The objective of this research to determine rSOD S. equorum ability to increase collagen deposition using fibroblast 3T3 against UVA. The research was initiated by the confirmation of E. coli BL21(DE3) carrying pJExpressÂ®414-sod. rSOD was overproduced in E. coli BL21(DE3) by IPTG induction to a final concentration of 1 mM for 4 hours at 37oC. rSOD purification was done using an Ni-NTA affinity column with gradient imidazole concentrations for elution. Various concentrations of rSOD was added to fibroblast 3T3 cell culture after UVA irradiation to determine its role in collagen deposition. The effect of rSOD was analyzed by fibroblast viability using Alamar blue and collagen deposition with picro sirius red. The results showed that fibroblast cell viability exposed with UVA for 45 minutes in the presence of 4, 8, and 16 unit rSOD was not significantly affected, however, the collagen deposition was significantly increased about 3.02%; 20.03% and 35.75% respectively compared to cell without rSOD. This result indicates that rSOD is a good candidate for an anti photoaging agent.

Deteksi Molekuler Ekson 2 Gen Beta Globin pada Pasien Beta Talasemia Mayor di RSUD DR. Soeroto Ngawi menggunakan Metode Polymerase Chain Reaction- Single Strand Conformation Polimorfism Yahya Febrianto; Ana Indrayati; Elfahmi .
Jurnal Farmasi Indonesia Vol 15 No 1 (2018): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Beta thalassemia is a hereditary blood disorder that caused by genetic disorder ofglobin gene.The condition leads to red blood cell damage so regular blood transfusion isneeded.This study was aimed to determine the presence of mutations in exon 2 gene beta frombeta globin thalassemia patients using PCR-SSCP method. The DNA was isolated from 5samples and amplified using PCR. The amplified product was characterizaed usingelectrophoresis. Region 2 of beta globin was product of primer forward 4 and primer reverse 5with 350 bp of target size. The PCR products of each region then futher analyzed using SSCPmethod. There was indication of mutation in 1,2,3,and 6 samples whereas no mutation insample 5.Based on the results of research can be concluded that PCR-SSCP method can beused to determine the type and location of mutations in exon 2 genes β globin from βthalassemia.

DETEKSI MOLEKULER EKSON 1 GEN BETA GLOBIN PADA PASIEN TALASEMIA BETA MAYOR DI RSUD DR. SOEROTO NGAWI DENGAN METODE POLYMERASE CHAIN REACTION (PCR) Agus Supriadi; Ana Indrayati; Elfahmi Elfahmi
Jurnal Farmasi Indonesia Vol 14 No 2 (2017): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Talasemia is an autosumal recessive gene mutation caused by a lack of synthesis of hemoglobin-forming globin chains of blood with anemia-like symptoms will decrease the production of red blood cells, and should be treated with regular blood transfusions.This study aims to determine the presence of mutations in exon 2 gene beta globin thalassemia patients using PCR method, where 5 samples in DNA isolation and continued PCR amplification, the amplification result of electrophoresis region 2 beta globin gene Region II is the result of amplification of primary 4 and primer primer reverse 5 with a target of 350 bp, on PCR there is amplification or doubling of the desired DNA sequence based on the primary selection for the reaction, the PCR product of each of these regions which is subsequently performed by SSCP.In the SSCP result the five samples were found in one sample showing different ribbon pattern with the sample N, there was sample 6, while the remaining four samples, there were 1,2,3,5 showed three bands. All the samples showed different from the N sample pattern. This shows that all samples indicated there were mutation

Deteksi Molekuler Ekson 3 Gen Beta Globin pada Pasien Beta Talasemia Mayor di RSUD DR. Soeroto Ngawi menggunakan Metode Polymerase Chain Reaction- Single Strand Conformation Polimorfism Choirul Huda; Ana Indrayati; Elfahmi Elfahmi
Jurnal Farmasi Indonesia Vol 16 No 1 (2019): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Talasemia is a genetic disease that causes globin chain synthesis disorder, a major component of the hemoglobin molecule. With the synthesis of unbalanced globin chains will decrease the production of red blood cells. This study aims to determine the presence of mutations in exon 2 gene beta globin thalassemia patients using PCR method, where 5 samples in DNA isolation and continued PCR amplification, the amplification result of electrophoresis region 2 beta globin gene Region II is the result of amplification of primary 4 and primer primer reverse 5 with a target of 350 bp, on PCR there is amplification or doubling of the desired DNA sequence based on the primary selection for the reaction, the PCR product of each of these regions which is subsequently performed by SSCP. PCR-SSCP could analysis the mutation in exon 3 beta globin genes. In the PCR-SSCP result, there was no mutation in region 4 exon 3 in five samples but probably there was mutation in another regions.

Aktivitas Ekstrak Kasar Enzim Fibrinolitik Bakteri Bacillus cereus yang Diisolasi dari Air Hutan Mangrove Maroon Edupark Semarang secara In Vitro Rizky Bimantara HA; Ana Indrayati; Desi Purwaningsih
Jurnal Farmasi Indonesia Vol 19 No 1 (2022): Jurnal Farmasi Indonesia
Publisher : Fakultas Farmasi Universitas Setia Budi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/jfi.v19i1.1322

Fibrinolytic enzymes are enzymes that work by degrading fibrin in blood clots. Fibrinolytic enzymes are produced by many organisms, one of which is the bacterium Bacillus cereus from the water of the Maroon Edupark mangrove forest, Semarang. Isolation of fibrinolytic enzymes from bacteria is very important because bacteria are easy to grow, have a fast generation time, do not require a large area for cultivation, and are easily genetically modified so that it will be more economically profitable. In addition, fibrinolytic enzymes from natural ingredients have fewer side effects than synthetic fibrinolytic drugs. This study aims to determine the activity of the crude extract of the fibrinolytic enzyme B. cereus in lysis blood clots in vitro. The study began with confirmation of the presence of genes encoding fibrinolytic enzymes from bacteria B. cereus uses NCBI's popular resources 'nucleotide', identification of bacterial morphology in blood agar media, Gram staining, endospore staining, catalase and coagulase testing. Isolation of crude extract of fibrinolytic enzyme B. cereus is carried out by enzyme extraction. Protein concentration were determined using Bradford method and fibrinolytic activity test in vitro using fibrin plate media with nattokinase as positive control. The resulting clear zone shows the ability of the enzyme extract in degrading fibrin The results of the identification of the B. cereus bacterial gene uses the NCBI data base registered as the AprE gene. The results of the identification of gram and endospore staining, B. cereus is a Gram-positive bacterium and has endospores. Identification of bacteria on blood agar media indicates that B. cereus represents the group of the β-hemolysis. Catalase and coagulase test results show that the bacteria produce catalase and coagulase enzymes. Total protein concentration from crude extract of B. cereus obtained at 19.63 mg/mL. Fibrinolytic activity at concentrations of 20, 40, 80% was 2.54; 6.11; and 7.94 mm respectively. Based on the above results it can be concluded that the crude extract of fibrinolytic enzyme B. cereus has the potential to be developed as a natural fibrinolytic agent.

OPTIMIZATION OF LIQUID SOAP SOAP (Caesalpinia sappan L.) LIQUID ETHANOL EXTRACT WITH KOH, STEARIC ACID AND SITRATE ACID USING SIMPLEX LATTICE DESIGN METHOD AND THE EFFECT OF ANTIBACTERIA ON STAPYLOCOCUSUSUSCATC 25259 Nurhayati Nurul; Ana Indrayati; Mimiek Murrukmihadi
Jurnal Infokes Vol 9 No 2 (2019): Jurnal Ilmiah Rekam Medis dan Informatika Kesehatan
Publisher : Universitas Duta Bangsa Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47701/infokes.v9i2.826

The most widely used soap is soap from sodium salt, but potassium soap is a softer and more soluble soap in water, so potassium soap is made for special functions, such as liquid soap. Brazilin in secang wood extract (Caesalpinia sappan L.) has potential as an antioxidant and antibacterial agent. Secang wood dry powder was extracted with 96% ethanol, thickened and then extracts tested. Fourteen liquid soap formulas using 10% secang wood extract with variations of KOH base, stearic acid, and citric acid were tested for physical properties including viscosity, pH, and free alkali. The optimum formula liquid bath soap can inhibit S. aureus ATCC 25923 as large as 32.6 ± 0.75 mm. The optimum formula is obtained by the Simplex Lattice Design method, the results are analyzed by expert design software. The optimum formula was tested for physical properties then analyzed using one-sample t-test and irritation test. The optimum form of liquid bath extract of sabaun secang wood extract contains KOH 0.6121%, stearic acid 0.613% and citric acid 0.085% with physical properties of viscosity of 9.69, pH of 9.95 free alkali of 0.08. T test results showed no significant difference between the response of the physical properties of the experimental results and software predictions. The optimum formula for shower bath liquid wood extract does not irritate the skin.

Co-Authors Agus Supriadi Apriani, Dwi Wulan Ardina, Duanty Indi Choirul Huda DEBBIE SOEFIE RETNONINGRUM Desi Purwaningsih Dini Afriliza Elfahmi . Elfahmi Elfahmi, Elfahmi Endang Sri Rejeki Fachry Abda El Rahman Fransiska Leviana Ghani Nurfiana Fadma Sari H. Harapan Harapan H, Harapan Hervina Warninghiyun I. Ichsan Ichsan I, Ichsan Ika Purwidyaningrum Ilham Kuncahyo, Ilham Ismi Rahmawati Ismi Rahmawati Isna Jati Asiya Jason Merari Peranginangin Jeny Suci Anggraini, Aliffia Kurniasari, Fitri LARAS AJENG PITAYU Lukito Mindi Cahyo M. Andi Chandra M. Andi Chandra Mandasari, Mitha Oktavia Mario Hendrik Refwalu Merari P, Jason Mimiek Murrukmihadi Mustika Endah Pratiwi Nadea Sherly Widya Putri Nadea Niswah, Sukma Uswatun Niva, Oktiva Cheria Kairul Nopianti, Vivin Nurfitriyawatie Nurfitriyawatie Nurfitriyawatie Nurhayati Nurul Pamungkas, Bayu Aji Patandung, Rosliana Permatasari, Ensa Ayu Pinesti, Arum Pramukantoro, Ganet Eko Pratiwi, Yunia Pudiastuti RSP Puspitasari, Ismi Putri, Seprina Ratna Ika Yusuf Rendati, Hema Novita Reslely Harjanti Rini Setyowati, Rini Rizal Maarif R. Rizal Maarif Rukmana, Rizal Maarif Rizky Bimantara HA Sari, Annisa Nurdinia Senja, Salsadella Juwita Sukmadaja Asyarie Susilowati, Rizka Agustine Tita Novarini Titik Sunarni Tri Suciati Triyani Triyani VALENTINA YURINA W. Wienands Wienands W, Wienands Wiwi Kusumasari Wiwin Herdwiani Wulandari, Destik Yahya Febrianto Zahra, Iklila Zulfah, Khairina

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