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All Journal BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan Indonesian Journal of Jamu
Kartikawati, Andriana
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Health Professions Medicine & Pharmacology

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Pengaruh Teknik Ekstraksi Daun Ubi Jalar Ungu (Ipomoea batatas) dan Daun Jambu Biji (Psidium guajava) terhadap Aktivitas Antioksidan Br Sembiring, Bagem; Bermawie, Nurliani; Rizal, Molide; Kartikawati, Andriana
Jurnal Jamu Indonesia Vol. 5 No. 1 (2020): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jji.v5i1.184

Abstract

Daun ubi jalar ungu dan daun jambu biji dikenal sebagai tanaman obat dan telah banyak dimanfaatkan untuk membantu mengatasi berbagai masalah kesehatan. Kandungan senyawa bioaktif yang bermanfaat bagi kesehatan dipengaruhi oleh mutu bahan baku dan teknik ekstraksi. Penelitian ini bertujuan mendapatkan teknik ekstraksi terstandar daun ubi jalar ungu dan daun jambu biji Australia untuk menghasilkan aktivitas antioksidan yang tinggi. Tahap kegiatan meliputi penanganan bahan baku, analisis mutu simplisia, ekstraksi, dan pengujian aktivitas antioksidan. Kegiatan menggunakan Rancangan Acak Lengkap faktorial yang terdiri dari 2 faktor yaitu konsentrasi pelarut etanol (50, 70 dan 96%) dan lama ekstraksi (2, 3, 4 jam). Parameter yang diamati yaitu mutu simplisia, rendemen ekstrak dan aktivitas antioksidan. Hasil penelitian diperoleh kandungan flavonoid simplisia daun ubi jalar ungu sebesar 1,30% dan daun jambu biji Australia 2,72%. Rendemen ekstrak berkisar antara 8,4-68,2% dan 21,2-53,8%. Nilai IC50 untuk aktivitas antioksidan terkecil ekstrak daun ubi jalar ungu yaitu 33,34 bpj hasil ekstraksi dengan etanol 70% selama 2 jam dan daun jambu biji Australia nilainya 10,10 bpj hasil ekstraksi dengan pelarut etanol 96% selama 3 jam. Aktivitas antioksidan ekstrak daun jambu biji Australia lebih kuat dibandingkan ekstrak daun ubi jalar ungu. Konsentrasi pelarut berpengaruh terhadap rendemen ekstrak dan kekuatan aktivitas antioksidan.
Pertumbuhan Tunas Aksilar Tebu Kultivar 'AAS Agribun' dengan Kombinasi BAP dan NAA Secara In Vitro Fajar Hidayat, Muhammad; Kusumarini, Niken; Kartikawati, Andriana; Suhesti, Sri; Sa’adah, Ira Nailas; Kusrinah; Mas'ud, Abdurrahman
Biotik Vol 14 No 2 (2026): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v14i2.35501

Abstract

Sugarcane (Saccharum officinarum L.) is a strategic sugar-producing crop in Indonesia; however, conventional vegetative propagation is limited by slow multiplication rates and the risk of disease transmission. In vitro culture offers an alternative approach for rapid and disease free seedling production. This study aimed to evaluate the effect of Benzyl Amino Purine (BAP) and Naphthalene Acetic Acid (NAA) combinations on axillary shoot growth of sugarcane cultivar 'Agriculture Acclimated Sugarcane Agribun' (abbreviated as 'AAS Agribun') and to determine the most effective concentration for shoot initiation. The study was conducted from October 2025 to January 2026 at the Laboratorium Unit Pengelola Benih Unggul Pertanian (UPBUP), Pusat Perakitan dan Modernisasi Pertanian (BrMP) Perkebunan, Bogor, West Java. A completely randomized factorial design was applied using BAP (0–2.0 mg/L) and NAA (0–0.5 mg/L) on Murashige and Skoog (MS) medium. Observed parameters included percentage of surviving explants, time of shoot emergence, number of shoots, shoot height, and number of leaves. The results indicated that 0.5 mg/L BAP without NAA produced the most effective response for axillary shoot initiation, as reflected by higher explant survival and improved shoot growth compared to other treatments. This was evidenced by the earliest shoot emergence, observed at 1.75 Weeks After Culture (WAC), and the highest leaf number, averaging 0.96 leaves per plant. These findings suggest that low BAP concentration without auxin supplementation is optimal for early-stage shoot induction of sugarcane cultivar ‘AAS Agribun’. Keyword: Axillary shoots; Plant growth regulators; Sugarcane; Tissue culture.
Co-Authors Br Sembiring, Bagem Fajar Hidayat, Muhammad Kusrinah Mas'ud, Abdurrahman Molide Rizal, Molide Niken Kusumarini, Niken Nurliani Bermawie Sa’adah, Ira Nailas Sri Suhesti