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EKSPRESI BMP-2 PADA PEMBERIAN HYDROXIAPATITE XENOGRAFT DAN HYDROXIAPATITE TOOTH-DERIVED BONE GRAFT MATERIAL PADA SOKET MARMUT Fauzia, Malianawati; Wibisono, Poernomo Agoes; Maduratna, Ernie
E-Prodenta Journal of Dentistry Vol 3, No 2 (2019)
Publisher : Fakultas Kedokteran Gigi UB

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Abstract

Latar Belakang: hydroxyapatite telah menujukkan kemampuan untuk memicu regenerasi tulang padafungsinya sebagai scaffold sekaligus sebagai pembawa BMP-2 lokal. Hydroxyapatite toothgraft memiliki rasioCa/P lebih tinggi dibanding hydroxyapatite xenograft sehingga lebih banyak jumlah BMP-2 yang dapatdiserap. Tujuan: untuk membandingkan jumlah ekspresi BMP-2 antara hydroxyapatite toothgraft denganhydroxyapatite xenograft. Metode: sebanyak 33 ekor marmut jantan dilakukan ekstraksi pada gigi insisifmandibula kiri, kemudian dibagi dalam tiga grup. Grup A (n=11): soket post ekstraksi diisi denganhydroxyapatite xenograft, grup B (n=11): soket post ekstraksi diisi dengan hydroxyapatite toothgraft, dangrup C (n =11): soket post ekstraksi tidak diisi bahan graft. Setelah 14 hari marmut dikorbankan kemudiansample dianalisis menggunakan kit IHC. Hasil: Data dianalisis menggunakan Krukal-Wallis test untukmembandingkan masing-masing grup (α = 0,05). Mean ekspresi BMP-2 pada hydroxyapatite xenograftadalah 15,73±2.649, pada hydroxyapatite toothgraft adalah 23,55±3.236, dan pada grup kontrol adalah 9,18 ±2,272. Kesimpulan: ekspresi BMP-2 pada hydroxyapatite toothgraft lebih banyak dibandinghydroxyapatite xenograft.
Effects of Lime (Citrus aurantifolia Swingle) Peel Extract Addition to Periodontal Dressing on the Number of Blood Vessels in the Gingival Wound Healing Process Fauzia, Malianawati; Oktaviana, Savira Pratista
Journal of Dentistry Indonesia Vol. 29, No. 2
Publisher : UI Scholars Hub

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Abstract

Background: Periodontal dressings that are currently available can only protect wounds from mechanical trauma, but have no ability to accelerate wound healing. Lime (Citrus aurantifolia Swingle) peel extract added to periodontal dressing contains flavonoids which can stimulate the formation of new blood vessels, thus accelerating the healing process. Objective: To determine the effects of adding lime (Citrus aurantifolia Swingle) peel extract to periodontal dressing on the number of blood vessels in the gingival wound healing process. Methods: 32 rabbits with 2 mm wound made on the gingiva were divided into 8 groups. For the control groups of K1(n=4) and K2(n=4) periodontal dressings were applied without the addition of lime peel extract. Meanwhile, the treatment groups P1(n=4) and P4(n=4), P2 (n=4) and P5(n=4), and P3(n=4) and P6(n=4), 5%, 10%, and 15% lime peel extract, respectively, were added to the periodontal dressing. Groups K1, P1, P2, and P3 were scarified after 3 days, whereas groups K2, P4, P5, and P6 were scarified after 5 days. Histological observations of tissue by Hematoxylin Eosin (HE) staining were carried out to count the number of blood vessels. Result: The results of the statistical tests show that group P5 had the highest number of new blood vessels. Conclusion: The addition of lime peel extract to periodontal dressing can increase the number of blood vessels in the gingival wound healing process.
E-Books in Improving Knowledge, Attitudes and Behavior about the Dangers of Smoking Fauzia, Malianawati; Widodorini, Trining; Handayani Lodra, Ester
International Journal of Community Service Learning Vol. 9 No. 1 (2025): February
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ijcsl.v9i1.86275

Abstract

The prevalence of smoking among adolescents in Indonesia, especially in Malang, is still quite high, with many adolescents starting to smoke at a young age. Low knowledge about the dangers of smoking is one of the factors that encourage smoking behavior among adolescents. This study aims to evaluate the effectiveness of using e-books as an educational medium in increasing students' knowledge about the dangers of smoking. This study used a quasi-experimental design with a pretest-posttest approach. The study sample consisted of 41 students of SMP Muhammadiyah 1 Malang who were selected using a purposive sampling technique. Data were collected through questionnaires before and after the intervention in the form of providing an e-book entitled "The Dangers of Smoking in Adolescents and How to Avoid It". Data analysis was carried out using SPSS software with statistical tests to compare changes in knowledge levels before and after the intervention. The results showed a significant increase in student's knowledge levels and positive attitudes toward the dangers of smoking after reading the e-book. These findings indicate that e-books can be an effective educational medium in increasing adolescents' understanding of the dangers of smoking, as well as supporting government efforts to reduce the number of smokers among the younger generation.
Effect of tooth graft particle size on the healing process of femur defects in Wistar rat (Rattus norvegicus) Fauzia, Malianawati; Kumala, Ega Lucida Chandra; Brilliando, Okky Satria; Ayu, Devi Amelia; Napitu, Theresia Agata Winnitya
Dental Journal (Majalah Kedokteran Gigi) Vol. 58 No. 4 (2025): December
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v58.i4.p388-394

Abstract

Background: Teeth have potential as bone graft materials because of their organic and inorganic components that can stimulate osteoinduction, osteoconduction, and osteogenesis. An important success indicator of treatment using this graft material is the formation of osteoblast and new blood vessels in the applied area. Purpose: To investigate the number of osteoblast, osteoclast, and new blood vessels in bone healing after the implantation of tooth-derived bone graft materials measuring 20, 40, and 60 mesh. Methods: Thirty-six Wistar rats with a 2 mm defect on the right femoral dextra condile were divided into four groups. P0 (n=9) was the control group, where the defect was not filled by any material. In the other groups, the defects were filled by 20-mesh (P1; n=9), 40-mesh (P2; n=9), and 60-mesh (P3; n=9) tooth graft material. The Wistar rats were sacrificed after 2 weeks, and then the preparations were hematoxylin eosin staining. The data were analyzed using one-way analysis of variance and Tukey’s post hoc test. Results: The highest number of osteoblast was in the P3 group with a mean of 49.67, highest number of new blood vessels in the P2 group with a mean of 39.89, and highest number of osteoclast in the P1 group with a mean of 20.44. Statistical analysis showed a significant difference in the number of new blood vessels, osteoclast, and osteoblast in each group (p=0.000; p<0.05). Conclusion: Particle size differences in tooth graft material affect osteogenesis and angiogenesis in the bone healing process.
Education on Smoking Dangers for Dental and Oral Health Among Students Widodorini, Trining; Fauzia, Malianawati; Lodra, Ester Handayani; Rafie, Mahardhika Ali; Hamidullah, Achmad Maulidana; Nayola, Adelia Marta; Adilah, Hanifa Fairuz; Wijanarko, Grace Vanesa; Alifah, Nisrina Andini; Fasa, Fathina Kamilia; Winara, Jesselyn; Aulia, Nabila Rizky Akbar
Caring: Jurnal Pengabdian Masyarakat Vol 5, No 2 (2025): CARING Jurnal Pengabdian Masyarakat (Agustus 2025)
Publisher : Caring: Jurnal Pengabdian Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.caringjpm.2025.005.02.2

Abstract

Background: Cigarettes contain thousands of harmful chemicals that can cause oral health problems such as tooth discoloration, halitosis, gingivitis, and oral cancer. WHO (2019) reported that 19.2% of Indonesian adolescents aged 13–15 are smokers, while Riskesdas (2018) found 57.6% of Indonesians have oral health issues. Early education is essential to raise awareness of smoking hazards. Objective: To improve students’ understanding of the effects of smoking on oral and dental health at SMP Muhammadiyah 01 Malang. Methods: Conducted on July 17, 2024, this activity involved 7th–9th grade students using a pre-test and post-test design to measure knowledge before and after education delivered via video and presentation. Results: Knowledge scores increased significantly after the intervention, with most students showing good to adequate understanding. Conclusion: Health education effectively enhances students’ awareness of the dangers of smoking and the importance of oral health care.
The impact of varying concentrations of hyaluronic acid gel on the enhancement of VEGF and angiogenesis expression Fauzia, Malianawati; Kumala, Ega Lucida Chandra; Aufani, Maulida; Syalisha, Addini Zoya; Wahyu, Adellia; Mukhlis, Muhammad Maulana Zikrullah
Majalah Kedokteran Gigi Indonesia Vol 11, No 2 (2025): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.104632

Abstract

Periodontal dressings can contribute to plaque accumulation, which in turn may cause inflammation, irritation of healing tissues, bacteremia, and difficulties in eating. Advances in knowledge and technology have improved the materials used in these dressings, aiming to enhance effectiveness and minimize complications. High-molecular-weight hyaluronic acid (HMW-HA) is a promising dressing material because of its anti-inflammatory properties and its ability to promote epithelial regeneration. This study aimed to determine whether different concentrations of HMW-HA gel influence vascular endothelial growth factor (VEGF) expression and angiogenesis during gingival wound healing in white rats (Rattus norvegicus) of the Wistar strain. A total of 42 rats were divided into seven groups: a negative control group (K1), a positive control group (K2) treated with 0.2% Gengigel®, and five treatment groups (P1–P5) receiving HMW-HA gel at concentrations of 0.2%, 0.4%, 0.6%, 0.8%, and 1%, respectively. Standardized 2 mm wounds were created with a punch biopsy on the lower anterior jaw of each rat. Treatments were administered twice daily for seven days. VEGF expression and angiogenesis were evaluated using immunohistochemical and hematoxylin–eosin staining. The group treated with 1% HA gel (P5) showed the highest average VEGF expression and angiogenesis compared with the negative control and lower-concentration treatment groups. These findings suggest that topical application of HMW-HA gel may enhance gingival wound healing in periodontal surgery by promoting angiogenesis and increasing VEGF expression.
The effect of nanoparticle tooth grafts on osteoblast stimulation in the first stages of the bone healing process in Wistar rats compared to the micro-tooth graft technique Kumala, Ega Lucida Chandra; Fauzia, Malianawati; Junivianti, Hana Salsabila
Dental Journal (Majalah Kedokteran Gigi) Vol. 56 No. 3 (2023): September
Publisher : Faculty of Dental Medicine, Universitas Airlangga https://fkg.unair.ac.id/en

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/j.djmkg.v56.i3.p184-188

Abstract

Background: The use of a bone graft in bone regeneration is challenging. Tooth graft material has been used as a bone graft alternative due to its similar composition of organic and inorganic materials close to the bone. Recently, nanotechnology has been used to improve bone graft quality. The osteoconduction rate in the defect area represents the bone graft quality. Purpose: This study aimed to compare the number of osteoblasts using nano-tooth grafts and micro-tooth grafts in Wistar rats. Methods: Wistar rats were divided into six groups: the negative control groups (examined on days 7 and 14), the micro-tooth graft groups (examined on days 7 and 14), and the nano-tooth graft groups (examined on days 7 and 14). The control group received nothing, the micro-tooth group received a micro-size tooth graft, and the nano-tooth graft group received a nano-size tooth graft on the injured femur. Histological observations of osteoblasts were carried out using a light microscope with 1000x magnification. Data were analyzed using one-way analysis of variance and least significant difference tests. Results: On day 7, the nano-tooth graft group showed a higher osteoblast number (11.75) than the micro-tooth graft group (7.5) (p = 0.039). There was no significant difference in the micro-tooth graft group compared to the control (p > 0.05). On day 14, the nano-tooth graft group showed a decrease in osteoblast number close to normal (control) (p > 0.05), while the micro-tooth graft group still experienced significant elevation. Conclusion: Nano-tooth grafts accelerate the stimulation of osteoblasts in the first stages of the healing process compared to micro-tooth grafts.