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All Journal The Indonesian Biomedical Journal Indonesian Journal of Perinatology
Prayudi, Pande Kadek Aditya
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Nursing Public Health

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HPV18 E6/E7 Mutation and Their Association with The Expression Level of Tumor Suppressor Proteins p53 and pRb among Indonesian Women with Cervical Cancer Mahendra, I Nyoman Bayu; Prayudi, Pande Kadek Aditya; Dwija, Ida Bagus Nyoman Putra
The Indonesian Biomedical Journal Vol 17, No 1 (2025)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v17i1.3400

Abstract

BACKGROUND: The E6/E7 mutation contributes to the intra-typic variant of HPV18 which may differ in their oncogenic potential. E6 and E7 target the tumour suppressor protein p53 and pRb, respectively, and their degradation play a crucial role in cervical carcinogenesis. However, the prevalence of HPV18 E6/E7 variants among Indonesian women with cervical cancer has not been elucidated. Therefore, this study was conducted to characterize the HPV18 variants among Indonesian women with cervical cancer and their association with tumor suppressor protein p53 and pRb.METHODS: A hundred Indonesian women with pathologically proven cervical cancer were consecutively recruited into the study. Polymerase chain reaction (PCR) was performed to detect HPV18 DNA E6 and E7 oncogenes using specific primers and the variants was determined through nucleotide sequencing. Expressions of p53 and pRb were analyzed through immunohistochemistry by using specific antibodies targeting p53 and pRB.RESULTS: The rate of HPV18 positivity was 24%. The rate of E6 and E7 mutation was 45.4% and 59.1%, respectively. Those with E6 mutation had significantly higher expression of p53 and pRb as compared to those with wildtype E6 (p<0.05). Subjects with E7 mutation only had higher expression of pRb (p<0.05). Phylogenetic analysis revealed that 54.5% subjects had genetic sequences closely related to Asian lineages, particularly A1, A4, and A5 sublineage. Interestingly, 3 subjects had genetic sequences closely related to MK813921, a newly identified sequences. However, 45.5% subjects had distinct genetic sequences that did not related to the reference sequence used in this study.CONCLUSION: E6 and E7 mutation was common among Indonesian women with HPV18 cervical cancer and associated with the level of tissue p53 and pRb expression.KEYWORDS: HPV18 E6/E7, mutation, epidemiology, Indonesian women
Characteristics of cervical cancer screening using visual inspection with acetic acid and pap smear in the outpatient clinic at Prof. Dr I.G.N.G. Ngoerah Hospital Denpasar from January to December 2023 Aryana, Made Bagus Dwi; Surya, I Gede Ngurah Harry Wijaya; Winata, I Gde Sastra; Ongko, Eric Gradiyanto; Budiana, Nyoman Gede; Mahendra, I Nyoman Bayu; Saspriyana, Kade Yudi; Darmayasa, I Made; Prayudi, Pande Kadek Aditya
Indonesian Journal of Perinatology Vol. 6 No. 2 (2025): Available online : 1 December 2025
Publisher : The Indonesian Society of Perinatology, South Jakarta, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51559/inajperinatol.v6i2.67

Abstract

Introduction: According to GLOBOCAN 2022, cervical cancer is the fourth leading cause of cancer-related morbidity and mortality among women worldwide, with 662,044 new cases and 348,709 deaths. In Southeast Asia, it ranks third in incidence and fourth in mortality. Indonesia contributes more than 50% of new cases (36,964) and deaths (20,708) in the region. Cervical cancer is largely preventable through early detection methods such as visual inspection with acetic acid (VIA) and Pap smear. However, differences in diagnostic accuracy between these screening methods highlight the need to identify patient characteristics appropriate for each modality. This study aimed to compare the characteristics of patients undergoing VIA and Pap smear screening at the outpatient clinic of Prof. Dr I.G.N.G. Ngoerah Hospital, Denpasar, from January to December 2023. Methods: This observational analytical study used secondary data obtained from outpatient clinic registers and medical records of female patients who underwent VIA or Pap smear screening during the study period. Data were analysed using univariate analysis. Results: Among 122 participants undergoing VIA, 24 (19.6%) had positive results. Pap smear examinations in 125 participants showed CIN I in 10 (8.0%), CIN II in 4 (3.2%), and CIN III in 5 (4.0%) participants. Vaginal discharge was reported in 7 VIA participants (5.7%) and 15 Pap smear participants (12.0%), while postcoital bleeding was reported in 2 participants (1.6%) in each group. Among VIA-positive cases, lesions were most commonly found in all four quadrants (45%). Conclusion: Patient characteristics in VIA and Pap smear screening at Prof. Dr I.G.N.G. Ngoerah Hospital varied considerably, with a higher proportion of positive results observed in VIA compared to Pap smear screening.
Co-Authors Aryana, Made Bagus Dwi Budiana, Nyoman Gede Dwija, Ida Bagus Nyoman Putra I Ketut Suwiyoga I Made Darmayasa KADE YUDI SASPRIYANA Nyoman Bayu Mahendra Ongko, Eric Gradiyanto Surya, I Gede Ngurah Harry Wijaya