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All Journal Padjadjaran Journal of Dentistry
Prijono, Eddy
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Antibacterial activity testing of ethanolic extract of aloe vera leaf and gel against methicillin-resistant Staphylococcus aureus Pratiwi, Tias Eka; Dewi, Warta; Prijono, Eddy
Padjadjaran Journal of Dentistry Vol 26, No 2 (2014): July 2014
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol26no2.26702

Abstract

Introduction: The interest on Aloe vera based herbal products is fast growing during recent years, particularly in medication regarding their antibacterial properties which had a lot of active components such as saponin and anthraquinone. Methicillin-resistant Staphylococcus aureus has emerged as one of the most important human pathogens causing nosocomial infections and became the first priority as a consequence of their resistancy. The purpose of this study is to determine the minimum levels of ethanol extract of leaves and Aloe Vera gel that can inhibit MRSA and find out whether there are differences in antibacterial power between of ethanol extract of leaves and Aloe Vera gel. Methods: Ten isolates of MRSA were investigated for their sensitivity to Aloe vera leaf and gel extract using the serial dilution method by doing two times repetition and statistically analyzed according to t-test method. Result: The result shows that the Minimum Inhibitory Concentration of Aloe vera leaf and gel extract each located at a concentration of 25% and 12,5%. There is a significant difference in antibacterial power between the ethanol extract of leaves and Aloe Vera gel in inhibiting MRSA with MIC values of 25% and 12.5% Conclusion: There was a difference between Aloe vera leaf and gel extract antibacterial activity, where the gel extract is more effective than leaf extract.
Effect of ultraviolet light irradiation on the growth of Candida albicans and the transversal strength of acrylic plate Widodo, Teguh Tri; Ardan, Rachman; Prijono, Eddy
Padjadjaran Journal of Dentistry Vol 22, No 3 (2010): November 2010
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol22no3.26897

Abstract

Introduction: Acrylic plates are the basis for several tools used in the oral cavity, including dentures and obturators. In its function, the acrylic plate is always in contact with the oral mucosa, so that good disinfection is needed without reducing the strength of the acrylic plate. Disinfection can be done by ultraviolet irradiation. This study is aimed to analyze the effect of ultraviolet light irraying on the growth of Candida albicans and the strength of transversal acrylic plate. Methods: This research is an experimental laboratory research with Replication Random Design. The disinfection success test was carried out by observing the population of the Candida albicans colony, while the acrylic plate transversal strength test was carried out on 35 acrylic heat curing plates tested according to ADA specifications. Results: Ultraviolet light can inhibit the growth of Candida albicans colonies with an optimum irradiation time of 20 minutes at a wavelength of 254nm. Meanwhile, ultraviolet irradiation on acrylic plates between 5-30 minutes shows a significant transverse strength. Conclusion: Ultraviolet light at appropriate wavelengths and times has been shown to significantly inhibit bacterial colony growth and significantly increase the transverse strength of acrylic plates.
Co-Authors Pratiwi, Tias Eka Rachman Ardan, Rachman Teguh Tri Widodo, Teguh Tri Warta Dewi, Warta