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All Journal MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Jurnal of Syiah Kuala Dentistry Society
Warta Dewi, Warta
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Anti-fungal capacity of Saga leaf (Abrus Precatorius L) towards Candida Albicans testing NA, Muslim Paranto; Harjadinata, Karlina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 19, No 2 (2007): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.535 KB) | DOI: 10.24198/pjd.vol19no2.14180

Abstract

The aim of this study was to evaluate the antifungal effect, by determining the Minimum Inhibitory Concentration (MIC) of saga leave extract against Candida albicans, as a causal of oral candidiasis. The saga left was extracted following maceration method. Candida albicans, as a tested fungal was isolated from patients at the Oral and Dental Hospital Faculty of Dentistry Universitas Padjadjaran. They were cultured on Sabouraud Agar plate, incubated at 37°C for 18-24 hours. The isolation and the identification of Candida albicans were based on colonial morphology and the fermentation of glucose, maltose, saccharose, dan lactose at the Microbiology Laboratory. This experimental laboratory study was conducted according to a serial dilution method from 16 mg/ml up to 0,25 mg/ml of saga leave extract with two-fold dilution in three repetitions. The result showed that the Saga leaves extract to be able to inhibit Candida albicans at minimum 2 mg/ml concentration. As a conclusion, the concentration of 2 mg/ml or more of saga leave extract has antifungal effect against Candida albicans.
Denture adhesive antifungal potency towards the growth of Candida Albicans Dewi, Siti Utami; Sumarsongko, Taufik; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 23, No 3 (2011): November
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2234.583 KB) | DOI: 10.24198/pjd.vol23no3.14036

Abstract

Denture adhesive is a device that applied to the base of a denture before the denture is inserted into the mouth. The device used to improve denture retention and stabilization. It was thought that added antifungal agent to denture adhesive might be an effective way to inhibit the growth of Candida albicans and prevent denture stomatitis.The study was performed as an experimental laboratory study by an observed antifungal test of denture adhesive against Candida albicans growth using Kirby Bauer diffusion agar method with 5 samples and 3-time repetitions. The conclusion of this study was that the antifungal agent in denture adhesive containing poly (methylvinylether/maleic acid), sodium-calcium mixed partial salt and propyl hydroxybenzoate and denture adhesive containing sodium carboxymethylcellulose couldnt inhibit the growth of Candida albicans.
The decreased of Streptococcus Mutans growth after topical application of phosphopeptide amorphous calcium phosphate paste Araf, Tika Faradina; Gartika, Meirina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 23, No 2 (2011): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (651.087 KB) | DOI: 10.24198/pjd.vol23no2.14023

Abstract

Casein Phosphopeptide-Amorphous Calcium Phosphate (CPP-ACP) paste is a topical application substance that consisted of a series of milk derivative peptide as a result of phosphorylation and has an antibacterial activity. The objective of this research was to find out the difference of Streptococcus mutans growth before and after CPP-ACP paste given topically to childs teeth. The method of the research was a quasi-experiment. Research samples were 10 students of MI Al Falah Islamic Boarding School, Jatinangor, West Jawa Indonesia and collected with purposive sampling technique. This research used dental plaque from childs teeth before and after applicated by CPP-ACP paste. The plaque was cultivated in selective media Tryptone Yeast Cysteine Sucrose Bacitracin (TYCSB) with repeated twice. Streptococcus mutans colony in TYCSB were counted by Stuart colony counter and statistically analyzed based on paired t-test. The results showed the average of Streptococcus mutans growth before applicated CPP-ACP paste was 57.05, whereas after applicated CPP-ACP paste for 1 days was 9.4; for 3 days was 2.85, and for 14 days was 1.7 colony. The research concluded that there was a decrease of Streptococcus mutans growth in isolate plaque after CPP-ACP paste topically given to childs teeth.
Minimum inhibition concentration and anti-fungal contact time of quaternary ammonium and ethylenediaminetetraacetic acid (EDTA) mixture towards Candida Albicans isolate Yunita, Elizabeth; Hardjawinata, Karlina; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 20, No 2 (2008): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (384.788 KB) | DOI: 10.24198/pjd.vol20no2.14140

Abstract

The aim of this study is to determine the Minimum Inhibitory Concentration (MIC) and the exposure time of the combination of quaternary ammonium compound with EDTA towards Candida albicans isolates from the 5 upper acrylic removable complete dentures. This experimental laboratory study was conducted based on a serial dilution of the combination of quaternary ammonium compound with EDTA towards Candida albicans in 3 replications and statistically analyzed according to Kruskal-Wallis method. The result showed that the MIC of the combination of quaternary ammonium compound with EDTA towards Candida albicans was in 1/8000 concentration with minimum 8 hours exposure time. This study concluded that the combination of quaternary ammonium compound with EDTA had an antifungal activity towards Candida albicans at 1/8000 concentration in 8 hours exposure time.
Antibacterial effect of pineapple (Ananas comosus) extract towards Staphylococcus aureus Loon, Yong Ker; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 30, No 1 (2018): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (429.911 KB) | DOI: 10.24198/pjd.vol30no1.16099

Abstract

Introduction: Staphylococcus aureus is one of the important medical pathogens which have been recognised for many years as a remedyfor a wide case of oral infections. Nowadays, the use of herbal remedy for reducing bacteria in the oral cavity has been implemented widely due to thefewer side effects. Therefore, researchershave been findingwaysto use pineapple in dentistry to prevent many cases of oral diseases. The purpose of this study was to prove that pineapple extract indifferent concentration had the potential as an antibacterial agent towards Staphylococcus aureus. Methods: The study was an experimental laboratory conducted by determining the minimum inhibitory concentration of pineapple (Ananas comosus) with thetwo-fold serial dilution methods. Results:The The Minimum Inhibitory Concentration (MIC) of the pineapple extract was 1.56%-0.78%. Conclusion: The pineapple extract had anantibacterial effect towards Staphylococcus aureus due to the bromelain compound and its phytochemical factor such as Vitamin C and flavonoid. 
Antibacterial effect of clove (Eugenia aromaticum) oil extracted from clove cigarettes towards Streptococcus mutans bin Jumali, Ahmad Alif Wazir; Satari, Mieke Hemiawati; Dewi, Warta
Padjadjaran Journal of Dentistry Vol 25, No 1 (2013): March
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.426 KB) | DOI: 10.24198/pjd.vol25no1.15428

Abstract

Streptococcus mutans is a commensal bacteria of the oral cavity, particularly found in dental plaque attached to the tooth surface, and can also found in the saliva, buccal mucosa, tongue, and the gingival sulcus. Clove cigarette contains the clove oil used worldwide as a herbal remedy for a variety of health disorders due to its antibacterial, antifungal, and antiviral properties. The purpose of this study was to determined the antibacterial effect of the clove oil contained in the clove cigarette towards Streptococcus mutans as a cariogenic bacteria. The research was an experimental laboratory, which tested the sensitivity of the Streptococcus mutans bacteria taken from the saliva of 10 clove cigarette smokers towards the clove oil extract of the clove cigarette in-vitro by using the Kirby-Bauer method. The study showed that the clove oil extract of the clove cigarette which contained eugenol has antibacterial towards the growth of Streptococcus mutans, and there was a difference of the antibacterial activity between clove oil extracted from minced and combusted clove cigarette. The conclusion of this study was the clove oil extracted from minced clove cigarette had a better antibacterial effect than the combusted clove cigarette.
Pemanfaatan ekstrak etil asetat buah merah sebagai zat pengganti pewarna primer pada teknik pengecatan tunggal bakteri gram negatif batangUtilization of ethyl acetate extract of Pandanus conoideus lam. as substitution for simple staining techniques of gram-negative rods bacteria Rahayuningtyas, Achsanul Dzuriyati; Dewi, Warta; Sudjarwo, Indrati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 2 (2017): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (739.009 KB) | DOI: 10.24198/jkg.v29i2.18583

Abstract

Pendahuluan: Buah merah merupakan salah satu buah yang banyak terdapat di Indonesia, terutama di daerah Papua. Buah merah (Pandanus conoideus Lam) mengandung karoten dan betakaroten yang menyebabkan warna merah yang sangat pekat pada buah merah. Tujuan penelitian untuk memanfaatkan ekstrak etil asetat buah merah sebagai pengganti zat pewarna primer bakteri gram negatif batang pada teknik pengecatan tunggal. Metode: Penelitian dilakukan secara deskripstif dengan cara melakukan teknik pengecatan tunggal. Pertama, dibuat biofilm tipis Escherichia coli sebagai objek penelitian pada kaca preparat, kemudian ekstrak etil asetat buah merah diteteskan diatasnya sebagai penganti zat warna karbol fuchsin (pewarna primer). Hasil pengecatan tunggal dilihat di bawah mikroskop dan data hasil pengecatan di tabulasikan atau dicatat pada tabel. Hasil: Ekstrak etil asetat buah merah tidak dapat mewarnai bakteri gram negatif batang. Simpulan: Ekstrak etil asetat buah merah tidak dapat digunakan sebagai zat warna pengganti pewarna primer pada proses pengecatan tunggal bakteri gram negatif batang. ABSTRACTIntroduction: Red fruit is one of fruits in Indonesia, especially in the Papua region. Red fruit (Pandanus conoideus Lam) contains carotene and betacarotene which causes a very thick red color on the red fruit. The purpose of this study was to utilize ethyl acetate extract of red fruit as primary dye substitute for simple staining techniques of Gram-negative rod bacteria. Methods: The study was carried out descriptively by doing a simple staining technique. First, a thin biofilm of Escherichia coli as a study object was made on a object glass, then the ethyl acetate extract of red fruit was dripped over it as a substitute for fuchsin carbolic dye (primary dye). The results of a simple staining are seen under a microscope and the results are tabulated or recorded in the table. Result: Ethyl acetate extract of Red fruit cannot stain gram-negative rod bacteria. Conclusion: ethyl acetate extract of red fruit cannot be used as a dye substitute for primary dye in the process of simple staining gram-negative rod bacteria.Keywords: Pandanus conoideus Lam, Escherichia coli, simple staining.
Pemanfaatan ekstrak etil asetat buah merah sebagai zat warna primer pada teknik pengecatan negatif kapsul bakteriUtilization of ethyl acetate extract of red fruit as primary negative staining substance for bacterial capsule Muthiah, Hishna; Dewi, Warta; Sudjarwo, Indrati
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 29, No 1 (2017): April
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.744 KB) | DOI: 10.24198/jkg.v29i1.18602

Abstract

Pendahuluan: Bakteri memiliki salah satu struktur sel yang penting untuk diamati dan dipelajari, yaitu kapsul. Kapsul merupakan salah satu struktur bakteri yang berkaitan erat dengan virulensinya pada manusia dan sel inang. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak etil asetat buah merah dapat digunakan sebagai alternatif pengganti zat warna primer pada teknik pengecatan negatif kapsul. Metode: Penelitian ini mendeskripsikan efektif atau tidaknya ekstrak etil asetat buah merah jika digunakan sebagai zat warna primer pada teknik pengecatan negatif kapsul. Hasil: Pewarnaan berhasil dilakukan. Hasil pewarnaan yang diperoleh selanjutnya disesuaikan dengan lembar degradasi warna berdasarkan RHS Colour Chart. Simpulan: Berdasarkan pengamatan diketahui bahwa penggunaan ekstrak etil asetat buah merah dapat mewarnai struktur bakteri dengan warna yang beragam dan mampu mendekati zat warna karbol fuksin. ABSTRACTIntroduction: Bacteria have one of the important cell structures to be observed and studied, namely capsules. The capsule is a bacterial structure that is closely related to its virulence in humans and host cells. This study aims to prove that the red fruit ethyl acetate extract can be used as an alternative to primary dyes in the capsule negative staining technique. Methods: This study describes the effectiveness of the red fruit ethyl acetate extract if it is used as a primary dye in the capsule negative staining technique. Result: Coloring is successful. The coloring results obtained are then adjusted to the color degradation sheet based on the RHS Color Chart. Conclusion: Based on the observations it is known that the use of red fruit ethyl acetate extract can color the structure of bacteria with a variety of colors and is able to approach the fusion carbolic dye.Keywords: Ethyl acetate extract of red fruit, negative staining technique, bacterial capsule, primary staining substance.
Pelaksanaan teknik aseptik oleh dokter gigi di RSGM Universitas Padjadjaran sebagai upaya preventif infeksi silang pada masa pandemi COVID-19 Himmatie, Shiela Nurulhuda; Dewi, Warta; Setiawan, Asty Samiaty
MKGK (Majalah Kedokteran Gigi Klinik) (Clinical Dental Journal) UGM Vol 9, No 1 (2023)
Publisher : Fakultas Kedokteran Gigi, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mkgk.81013

Abstract

Kedokteran gigi merupakan bidang yang rawan terhadap terjadinya infeksi silang. Munculnya wabah penyakit infeksi COVID-19 meningkatkan kekhawatiran tenaga medis maupun pasien dalam penyebaran infeksi. Dokter gigi merupakan profesi yang berisiko tinggi terhadap penularan COVID-19 karena selama perawatan menghasilkan banyak aerosol maupun droplet yang infeksius. Teknik aseptik merupakan semua prosedur yang dilakukan untukmencegah atau meminimalisir risiko infeksi oleh mikroorganisme patogen pada pasien maupun tenaga medis selama prosedur klinis. Tujuan penelitian ini untuk mengetahui gambaran pelaksanaan teknik aseptik oleh dokter gigi di RSGM Unpad sebagai upaya preventif infeksi silang pada masa pandemi COVID-19. Jenis penelitian ini adalah deskriptif. Sampel diambil menggunakan teknik accidental sampling pada bulan Agustus-November 2022 yangterdiri dari 51 dokter gigi di RSGM Unpad yang memenuhi syarat untuk menjadi responden. Penelitian dilakukan dengan metode survey menggunakan kuesioner yang berisi 32 pertanyaan mengenai tindakan teknik aseptik yang harus dilakukan oleh dokter gigi di fasilitas pelayanan kesehatan gigi dan mulut saat sebelum dan selama masa pandemi COVID-19. Penggunaan masker bedah (92,2%), surgical scrub (86,3%), masker N95 (82,4%) termasuk kategori baik. Penerapan lima momen kebersihan tangan (70,6%), skrining COVID-19 (62,7%), desinfeksi seluruh permukaan dental unit dan benda di meja dental (60,8%), penggunaan goggle/faceshield (70,6%), hazmat (68,6%), dan shoe cover/sepatu boots (60,8%) termasuk kategori cukup baik. Penggunaan rubber dam (7,8%), sarung tangandouble (43,1%), dan penerapan flushing DUWL dan handpiece setiap pergantian pasien (45,1%) termasuk kategori kurang. Pelaksanaan teknik aseptik standar maupun teknik aseptik tambahan pada masa pandemi COVID-19 secara keseluruhan telah dilakukan dengan baik oleh sebagian besar dokter gigi di RSGM Unpad.
The antimicrobial effect of piper betel leaves extract against Streptococcus mutans Mohammad Subri, Liyana; Dewi, Warta; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 24, No 3 (2012): November 2012
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol24no3.26835

Abstract

Introduction: Betel leaves are herbal plant that is believed to have phenolic compound as an antimicrobial agent. One of the medical plants (herbal) which has an-timicrobial properties is Piper betel.The aim of this research is to analyzed the antimicrobial effect of Piper Betel Leaves extract against Streptococcus mutans. Methods: Type of this research is laboratory experiment. Population of this study are Piper betle Linn plant in West Java. The MIC test were conducted based on serial dilution method in 64%, 32%, 16%, 8%, 4%, 2%, 1%, 0.5%, 0.25%, 0.125% concentration of Piper betel leaves extracts against 5 sample of Streptococcus mutans within three repetitions. Results: The result shows that 0.25% concentration of Piper betel leaves extract can inhibit Streptococcus mutans. Conclusion: Piper betel leaves extract can inhibit the growth and had antimicrobial effect against Streptococcus mutans.
Co-Authors Amir, Arrahmi Asty Samiaty Setiawan, Asty Samiaty Balafif, Felisha Febriane bin Jumali, Ahmad Alif Wazir Elizabeth Yunita, Elizabeth Emma Rachmawati Hazhiyyah, Nabila Ghassania Himmatie, Shiela Nurulhuda Jumali, Ahmad Alif Wazir Karlina Hardjawinata, Karlina Karlina Harjadinata, Karlina Loon, Yong Ker Loon, Yong Ker Meirina Gartika Mieke Hemiawati Satari Mohammad Subri, Liyana Muslim Paranto NA, Muslim Paranto Muthiah, Hishna Nafisa, Durotun Pratiwi, Tias Eka Prijono, Eddy Rahayuningtyas, Achsanul Dzuriyati Riana Wardani Selvi Marcellia Siti Utami Dewi, Siti Utami Sudjarwo, Indrati Taufik Sumarsongko Tika Faradina Araf, Tika Faradina