Article Per Year (5 Year)
p-Index From 2021 - 2026
0.23
P-Index
This Author published in this journals
All Journal International Journal of Renewable Energy Development Jurnal Teknik Kimia dan Lingkungan
Tashiro, Yukihiro
Unknown Affiliation
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Materials Science & Nanotechnology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Biobutanol Production Using High Cell Density Fermentation in a Large Extractant Volume Darmayanti, Rizki Fitria; Tashiro, Yukihiro; Sakai, Kenji; Sanomoto, Kenji; Susanti, Ari; Palupi, Bekti; Rizkiana, Meta Fitri
International Journal of Renewable Energy Development Vol 9, No 3 (2020): October 2020
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.2020.29986

Abstract

Biobutanol is well known as a suitable substitute for gasoline, which can be applied without engine modification. Butanol toxicity to the producer strain causes difficulties to grow strain of higher than 4 g/L dry cell weight and to produce butanol higher than 20 g/L. Fermentation using high initial cell density has been reported to enhance butanol productivity. In addition, oleyl alcohol has been recognized for effective extraction of butanol because of its selectivity and biocompatibility with reduced the effect of toxicity. Butanol fermentation with high cell density and large extractant volume has not been reported and is expected to improve butanol production in a minimum medium volume setting. Clostridium saccharoperbutylacetonicum N1-4, C. beijerinckii NCIMB 8052 (8052), and C. acetobutylicum ATCC 824 (824) were used in this study. Three kinds of media, TYA, TY, and TY-CaCO3, were used in this conventional extractive fermentation. Then, in situ extractive fermentation with Ve/Vb ratios at 0.1, 0.5, 1.0, and 10 were used. Total butanol concentration was defined as the broth-based total butanol, which is the total amount of butanol produced in broth and extractant per the volume of broth. TYA medium yielded the highest total butanol concentrations at N1-4 (12 g/L), 8052 (11 g/L), and 824 (15 g/L), and the highest partition coefficient (3.7) among the three media with similar Ve/Vb ratio at 0.5. N1-4 yielded the highest increment of total butanol production (22 g/L) in the extractive fermentation with high cell density. Low butanol concentration of 0.8 g/L in the broth was maintained using the extractant at a broth volume ratio (Ve/Vb) much lower than 4.4 g/L with a ratio of 0.5. Ve/Vb ratio of 10 which provided 2-fold higher total butanol concentration (28 g/L) than that of 11 g/L obtained using a Ve/Vb ratio of 0.5. These results indicated that a larger volume of extractant to broth improved total butanol concentration by reducing butanol toxicity and led to high medium based butanol yield in fermentation using high cell density. 
Techno-Economic Analysis of Extractive Butanol Fermentation by Immobilized Cells with Large Extractant Volume Darmayanti, Rizki Fitria; Muharja, Maktum; Zhao, Tao; Gao, Ming; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji
Jurnal Teknik Kimia dan Lingkungan Vol. 6 No. 2 (2022): October 2022
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.755 KB) | DOI: 10.33795/jtkl.v6i2.337

Abstract

There are several challenges for ABE fermentation to be used in an industrial scale including the low of butanol yield, the high energy requirement for separation and purification, and the competeness of sugar with food demand as substrat. In this study, techno-economical aspects of ABE fermentation by using immobilized cells with large extractant volume were studied. Overall production process was designed using rice straw as raw material which is semi-hydrolyzed to produce cellobiose, glucose, xylose, and arabinose mixture. Concentrated sugar was then fed to extractive fed-batch fermentation using immobilized cells. Finally, extractant was recovered and products were purified by distillation column. By evaluating this process design for the small scale capacity of 238 kg-butanol and acetone/day, the energy requirement was 41.3 MJ/kg-butanol and acetone and the cost was 1.91 $/kg-butanol and acetone. Although the cost was higher than butanol produced by petrochemical process of 1.08 $/kg-butanol, it may reduce if the scale is increased.
Co-Authors Ari Susanti Bekti Palupi KENJI SONOMOTO Meta Fitri Rizkiana Ming Gao Muharja, Maktum Rizki Fitria Darmayanti Sakai, Kenji Sanomoto, Kenji Tao Zhao