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KENJI SONOMOTO
Laboratory of Microbial Technology, Division of Microbial Science and Technology, Department of Bioscience and Biotechnology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan Laborato
Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Materials Science & Nanotechnology

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ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA Ambarsari, Hanies; Sonomoto, Kenji
Jurnal Energi dan Lingkungan (Enerlink) Vol 11, No 1 (2015)
Publisher : Jurnal Energi dan Lingkungan (Enerlink)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5600.892 KB)

Abstract

A series of experiments had been conducted to investigate the performance of Clostridiumsaccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, andethanol (ABE) using several substrates that have not been investigated previously using this strain,such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as morecomplex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly tostudy the effect of such various substrate types and concentrations, as well as the substrates mixtureson the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE fromvarious substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, exceptCMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could bethe more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the typeand concentration of substrates being used by strain N1-4.Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation
ACETONE-BUTANOL-ETHANOL FERMENTATION FOR BIOENERGY USING VARIOUS SUBSTRATES IN DEFINED TYA MEDIA Ambarsari, Hanies; Sonomoto, Kenji
Jurnal Energi dan Lingkungan (Enerlink) Vol 11, No 1 (2015)
Publisher : Badan Pengkajian dan Penerapan Teknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (5600.892 KB) | DOI: 10.29122/elk.v11i1.1590

Abstract

A series of experiments had been conducted to investigate the performance of Clostridiumsaccharoperbutylacetonicum strain N1-4 (ATCC 13564) in direct fermentation of acetone, butanol, andethanol (ABE) using several substrates that have not been investigated previously using this strain,such as cellobiose, dextrin, starches of corn, potato, tapioca, wheat, and sago, as well as morecomplex carbohydrates such as xylans, cellulose, and carboxyl methyl cellulose (CMC), particularly tostudy the effect of such various substrate types and concentrations, as well as the substrates mixtureson the ABE ratio and productivity. The results showed that strain N1-4 could directly produce ABE fromvarious substrates, from monosaccharide to polysaccharides of hexose or pentose sugars, exceptCMC and cellulose. The experiments also suggested that cellobiose (a hexose disaccharide) could bethe more efficient substrate for strain N1-4 than glucose (a monosaccharide) to produce ABE in the TYA(Tryptone Yeast Agar) medium. It was proven also that the ABE ratio was very dependent on the typeand concentration of substrates being used by strain N1-4.Keywords: acetone, butanol, ethanol, clostridium saccharoperbutylacetonicum N1-4, fermentation
Redesigning the Coagulation Process for Treating Water Produced from Petroleum Drilling in Water Treatment Injection Plants Muharja, Maktum; Darmayanti, Rizki Fitria; Sonomoto, Kenji
Jurnal Teknik Kimia dan Lingkungan Vol. 9 No. 1 (2025): April 2025
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33795/jtkl.v9i1.5156

Abstract

This study aims to optimize the coagulation process for treating wastewater produced from petroleum drilling. The research includes redesigning the flocculator to enhance the coagulation process. The initial quality of the wastewater, characterized by parameters such as turbidity and Total Suspended Solids (TSS), did not meet the environmental quality standards stipulated by the Indonesian government. Poly Aluminum Chloride (PAC) coagulant and Polyacrylic Acid (PAA) flocculant were utilized at varying doses of 10–150 ppm and 0.25–25 ppm, respectively. The study identified the key challenges in the current coagulation and flocculation processes, including inefficiencies in pollutant removal and high operational costs. Optimal doses of 10 ppm for coagulants and 0.25 ppm for flocculants were determined, resulting in daily operational cost savings of IDR 15,865,030. The economic analysis was conducted to project the potential cost savings based on the optimized dosages, indicating a reduction in chemical costs and operational expenses. Moreover, the optimal injection distance for PAA flocculant was found to be approximately 3.5 meters from the static mixer. A new design for the flocculator was proposed, incorporating these findings to improve the overall treatment efficiency. The redesigned flocculator prototype features a 6-meter-long horizontal tube, 1.5 meters in diameter, with 15 partitions spaced 40 cm apart, and a water level difference between the inlet and outlet of around 0.67 cm. These findings suggest that coagulation redesign and optimization, along with clarifier engineering, can significantly reduce operational costs and enhance water quality for injection into the earth.
Techno-Economic Analysis of Extractive Butanol Fermentation by Immobilized Cells with Large Extractant Volume Darmayanti, Rizki Fitria; Muharja, Maktum; Zhao, Tao; Gao, Ming; Tashiro, Yukihiro; Sakai, Kenji; Sonomoto, Kenji
Jurnal Teknik Kimia dan Lingkungan Vol. 6 No. 2 (2022): October 2022
Publisher : Politeknik Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (717.755 KB) | DOI: 10.33795/jtkl.v6i2.337

Abstract

There are several challenges for ABE fermentation to be used in an industrial scale including the low of butanol yield, the high energy requirement for separation and purification, and the competeness of sugar with food demand as substrat. In this study, techno-economical aspects of ABE fermentation by using immobilized cells with large extractant volume were studied. Overall production process was designed using rice straw as raw material which is semi-hydrolyzed to produce cellobiose, glucose, xylose, and arabinose mixture. Concentrated sugar was then fed to extractive fed-batch fermentation using immobilized cells. Finally, extractant was recovered and products were purified by distillation column. By evaluating this process design for the small scale capacity of 238 kg-butanol and acetone/day, the energy requirement was 41.3 MJ/kg-butanol and acetone and the cost was 1.91 $/kg-butanol and acetone. Although the cost was higher than butanol produced by petrochemical process of 1.08 $/kg-butanol, it may reduce if the scale is increased.
Co-Authors Hanies Ambarsari Ming Gao Muharja, Maktum Rizki Fitria Darmayanti Sakai, Kenji Tao Zhao Tashiro, Yukihiro