Article Per Year (5 Year)
p-Index From 2021 - 2026
0.444
P-Index
This Author published in this journals
All Journal The Indonesian Biomedical Journal Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Budiono, Bernardus Parish
Unknown Affiliation
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Andrographis paniculata Leaves Extract Inhibit TNF-α and Caspase-3 Expression of Septic Rats’ Intestinal Tissues Ardika, Ryco Giftyan; Budiono, Bernardus Parish; Widiastiti, Nyoman Suci; Maharani, Nani; Susilaningsih, Neni; Sandra, Ferry
The Indonesian Biomedical Journal Vol 16, No 1 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i1.2727

Abstract

BACKGROUND: Microcirculation and cellular disturbances caused by sepsis might trigger significant intestinal damage. Andrographis paniculata extract decreases inflammatory intestinal epithelial cells with its role as an antiparasitic and anti-inflammatory agent. However, A. paniculata extract’s effect on sepsis have not been commonly studied, especially in the intestinal tissues. Therefore, this study was conducted to determine A. paniculata leaves extract (APLE) effect in sepsis-induced intestinal tissues of rats by examining the expression of inflammatory cytokines involved in sepsis, namely tumor necrosis factor (TNF)-α and Caspase-3.METHODS: Rats were divided into five groups; two groups received no pretreatment and the other three groups received 200, 400, and 500 mg/kg BW/day APLE, respectively. Three pretreated groups and one group with no pretreatment were then injected with 1 mg/200 g BW lipopolysaccharides (LPS) intraperitoneally to create septic rat models. Three days after the LPS-induction, rats were euthanized and the expression of TNF-α and Caspase-3 were assessed based on the immunohistochemical staining of rats’ intestinal tissues.RESULTS: Compared with NaCl (sham), LPS significantly (p<0.001) induced TNF-α expression from 6.60±1.36 to 25.37±1.74. Pretreatment of 200, 400, and 500 mg/kg BW/day APLE could significantly (p<0.001) inhibit the LPS-induced TNF-α expression (18.82±1.36, 11.45±1.18, and 6.89±1.90, respectively). Similar with TNF-α, compared with NaCl (sham), LPS significantly (p<0.001) induced Caspase-3 expression from 6.92±1.66 to 23.59±2.25. Pretreatment of 200, 400, and 500 mg/kg BW/day APLE could significantly (p<0.001) inhibit the LPS-induced Caspase-3 expression (17.47±1.68, 12.99±1.51, and 5.59±1.51, respectively).CONCLUSION: The pretreatment of APLE could inhibit the LPS-induced TNF-α and Caspase-3 expression, therefore APLE could be suggested as a potential sepsis-preventing agent.KEYWORDS: Andrographis paniculata, sepsis, TNF-α, Caspase-3, lipopolysaccharide 
Terapi Herbal untuk Pencegahan Adhesi Peritoneal Pascaoperasi: Tinjauan Mekanistik Studi Praklinis Inzaghi, Ika Silviadewi; Mahati, Endang; Budiono, Bernardus Parish
JURNAL RISET KESEHATAN POLTEKKES DEPKES BANDUNG, Online ISSN 2579-8103 Vol 18 No 1 (2026): Jurnal Riset Kesehatan Poltekkes Depkes Bandung
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/juriskesbdg.v18i1.3201

Abstract

Background: Postoperative peritoneal adhesions remain a common complication of abdominal and pelvic surgery and may cause bowel obstruction, infertility, chronic pain, and difficult reoperation. Objective: This review synthesized evidence on herbal therapeutics for adhesion prevention. Methods: A comprehensive search of PubMed, Scopus, and Google Scholar for 2015-2025 publications used keywords related to postoperative adhesions and herbal therapy. English-language full-text in animal experimental studies with peritoneal adhesion induction and herbal interventions were included; non-herbal interventions, duplicate reports, non-postoperative adhesions, abstracts only, non-English papers, and retracted articles were excluded. Results: A total of 169 records were identified, and after duplicate removal, title and abstract screening, and full-text eligibility assessment, 29 studies were included in the qualitative review. Curcumin consistently reduced inflammatory signaling, oxidative stress, collagen deposition, and adhesion scores through NF-kB modulation and antioxidant effects. Ginger and gingerol lowered TNF-α, IL-6, TGF-β1, VEGF, and malondialdehyde, indicating anti-inflammatory, antifibrotic, and anti-angiogenic activity. Rosmarinus officinalis reduced cytokines, oxidative markers, and TGF-β1 in rat models. Bletilla striata, frankincense, and polyherbal formulas showed additional wound-healing, anti-inflammatory, and pro-fibrinolytic effects. Conclusion: Herbal therapeutics appear promising as adjunctive strategies for preventing postoperative adhesions; however, the evidence remains heterogeneous and is dominated by animal and in vitro studies. Standardized formulations, safety evaluation, and well-designed clinical trials are needed before wider clinical application.
Co-Authors Endang Mahati Ferry Sandra Inzaghi, Ika Silviadewi Nani Maharani Neni Susilaningsih Ryco Giftyan Ardika Widiastiti, Nyoman Suci