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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN Media Kesehatan Masyarakat Indonesia JURNAL GIZI INDONESIA Journal of the Medical Sciences (Berkala Ilmu Kedokteran) El-Hayah : Jurnal Biologi Jurnal Kesehatan Masyarakat Indonesian Journal of Cancer Chemoprevention Journal of Biomedicine and Translational Research The Indonesian Biomedical Journal Universa Medicina Biosaintifika: Journal of Biology & Biology Education YARSI Medical Journal Bioscientia Medicina : Journal of Biomedicine and Translational Research Medica Hospitalia Medica Arteriana (Med-Art) Journal of Telenursing (JOTING) Diponegoro International Medical Journal (DIMJ) Narra J Bioscientia Medicina : Journal of Biomedicine and Translational Research Life Science Jurnal Locus Penelitian dan Pengabdian JKKI : Jurnal Kedokteran dan Kesehatan Indonesia Indonesian Journal of Biomedicine and Clinical Sciences

Neni Susilaningsih

Anatomy Histology Department, Faculty Of Medicine, Diponegoro University, Semarang, Indonesia

Author-ID : 396354

Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Dentistry Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Neuroscience Nursing Public Health

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Pola kuman pada penderita Infeksi Saluran Kemihdi RSUP Dr Kariadi Semarang Joni Karjono, Bambang; Susilaningsih, Neni; Damma Purnawati, Ratna
Jurnal Kedokteran YARSI Vol 17, No 2 (2009): MEI - AGUSTUS 2009
Publisher : Lembaga Penelitian Universitas YARSI

Urinary Tract Infection (UTI) is one of the most common bacterial infections found in clinical practice. The incidence of UTI increases with age. Despite various causes, the main causal agent is Escherichia coli.The aim of this study is to observe the pattern of bacteria underlying UTI in Dr. Kariadi Teaching Hospital, Semarang. A cross sectional design study was conducted using urine samples collected from 195 patients being diagnosed as UTI. Urine samples were cultured and counted according to microbiology standard method. Data obtained were analyzed employing descriptive approach. The result showed that 79 individuals (40.5%) were suffering from UTI, consisting of 31.6% male and 68.4%women. Most of the UTI patients were due to E. coli (65.8%) followed by E.coli and S. aureus (10.1%), S.Aureus (8.9%), Enterobacter sp. (7.6%), E. coli and Pseudomonassp. (2.5%), Enterobacter sp.E. coli (2.5%), Enterococcus sp. (1.3%) and E.coli, S. aureus and Enterobacter sp. (1.3%). It was concluded that the most common cause of UTI was E. coli and women were more frequently affected than men.

PHYCOCYANIN FROM SPIRULINA PLATENSIS INDUCES CYTOTOXICITY AND APOPTOSIS IN T47D CELLS Dimarti, Safira Chairani; Susilaningsih, Neni; Yuniati, Renni
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Breast cancer cases in Indonesia have risen in alarming rate. Herbal medicines have been developed to complement conventional cancer therapy and to lessen the negative effects of the therapies. Phycocyanin, blue-green photosynthetic pigment from Spirulina platensis, becomes a potential candidate of herbal medicine for cancer therapy due to its capability to eliminate various cancer cell line on in vitro level. This research aimed to analyse the effectivity of phycocyanin to induce cytotoxic and apoptotic activity of T47D cells (breast cancer cell line). MTT assay was perform to measure IC50 value. Double-staining assay and flow cytometry were applied to visualize the appearance of apoptotic cells and to measure the percentage of apoptotic and necrotic cells. Result showed that the IC50 value of phycocyanin towards T47D cells is 1650 ?g/mL. Phycocyanin was also proven to induce apoptosis at IC50 with the apoptosis percentage of 45.93%. This research presented different results from previous researches that used phycocyanin from saltwater Spirulina platensis. Based on the results of this study, phycocyanin has the potential to be developed as anticancer agent by inducing apoptosis.

CYTOTOXICITY AND ANTIPROLIFERATION OF PHYCOCYANIN FROM SPIRULINA PLATENSIS EXTRACT ON WIDR COLON CANCER CELL LINE Putri, Ajeng Kurniasari; Dimarti, Safira Chairani; Yuniati, Renni; Susilaningsih, Neni
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020 Article-in-Press
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.22881

Phycocyanin from Spirulina platensis extract has anticancer activity against various types of cancer cell cultures. However study about its effect on colon cancer cell lines, especially the WiDr, has not been reported before. This study aimed to reveal the anticancer activity of phycocyanin from Spirulina platensis extract on WiDr cells. The research was an in vitro experimental study, with the investigation on cytotoxicity also antiproliferation as the anticancer parameters. Both cytotoxicity and antiproliferation test was conducted through MTT assay to observe the visualization and inhibition of proliferation of different concentrations of phycocyanin in several incubation times on the WiDr colon cancer cell line. The obtained data were then processed statistically with the Two Way ANOVA test at a significance value of p <0.05 and followed with the Post Hoc test since there were significant differences. Based on the results, it could be postulated that phycocyanin extracted from freshwater Spirulina platensis was classified as non-toxic (IC50 of 855 µg/ml). Consequently, it is less potential to be used as the treatment for colon cancer. However, phycocyanin could inhibit the proliferation of the WiDr cell for approximately 47.4%, specifically at the concentration of 1710 µg/ml for 72 hours. It could be concluded that freshwater phycocyanin is less effective as an anticancer substance. The benefit of this study is to provide the new scientific evidence of the contrary results of freshwater phycocyanin activity from Spirulina platensis as an anticancer agent of colon cancer.

Screening for Hypertension and Electrocardiography (ECG) for Cardiovascular Diseases Risk Armalina, Desy; Witjahjo, Bambang; Susilaningsih, Neni; Purnawati, Ratna Damma; Ismail, Akhmad; Saktini, Fanti
KEMAS: Jurnal Kesehatan Masyarakat Vol 15, No 3 (2020)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v15i3.17969

ECG is a routine, accessible, inexpensive, and non-invasive tool for diagnosis of CVD and has been commonly described in medical reports as a suitable diagnostic tool for assessing Ã¢â‚¬ËœsilentÃ¢â‚¬â„¢ heart disease. An observational descriptive study, in Gisikdrono Urban Communities, found 72% elderly with hypertension, and higher than the reported national prevalence in 2013 (25.8%). We gained 38% of elderly with abnormal ECG which is: 5% Old myocardial infarction, 3% IMO Anteroseptal, 5% LVH, 8% LAD, 3%) of Inferior Ischemia, 3% dysrhythmia, and 13% bradycardia, without complaint of heart disease before. Aging became one of the factors of declining protection in the heart and increases the risk of destruction of heart failure. Considering examination of other organs that can affect the cause of heart anomalies to the elderly and expected to raise awareness of the importance of maintaining the blood pressure.

Perbedaan Kadar IgA dan IL6 Pada Penumpang â€œBiosmart and Safety Busâ€ pada Memakai Masker Herbal dan Masker Medis Rahajeng, Herera; Prasetyo, Awal; Susilaningsih, Neni; Sadhana, Udadi; Maharani, Nani; Asri, Hairul
Life Science Vol 10 No 1 (2021): April 2021
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/lifesci.v10i1.47177

Viral infections that attack the respiratory tract are currently a major problem globally. The spread of viral infection can be one of the risks by passengers of the bus. The government's efforts being developed are the application of biosmart and safety buses designed to prevent the virus with stake holders. One of the health protocols is the use of masks. The purpose of this study was to see the differences in IgA and IL6 levels before and after the use of medical masks and herbal masks. Nasal wash samples were taken and analyzed by ELISA. The results obtained were the level of IgA for the control group before wearing medical masks was 23.46834 pg/ml and after wearing 29.74473 pg/ml. While the IL6 levels before were 1.10544 ng / ml and after -2.04119 ng/ml. The results of the IgA in the experimental group before using the herbal mask 24.00840 pg/ml and after using was 29.74473 pg/ml. Meanwhile, the level of IL6 before was 0.91682 ng/ml and after 0.500157 ng/ml. Based on the results, there was an increase in IgA levels after the use of medical masks and herbal masks while IL6 levels decreased after the use of medical and herbal masks. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6 Infeksi virus yang menyerang saluran pernapasan saat ini menjadi masalah utama secara global. Penyebaran agen infeksi virus dapat menjadi salah satu risiko yang dihadapi penumpang moda transportasi Bus. Upaya Pemerintah seperti yang saat ini dikembangkan adalah pengaplikasian biosmart and safety bus yang dirancang guna menghambat persebaran virus dengan stake holder yang tepat. Salah satu penerapan protokol kesehatan adalah penggunaan masker. Tujuan dari penelitian ini adalah melihat perbedaan kadar IgA dan IL6 sebelum dan sesudah pemakaian masker medis dan masker herbal pada penumpang biosmart and safety bus. Pengambilan sampel nasal wash dilakukan dan dianalisis dengan ELISA. Penelitian ini merupakan quasi experimental dengan prepost test randomized control design. Hasil yang didapat adalah kadar IgA untuk kelompok kontrol sebelum pemakaian masker medis 23,46834 pg/ml dan setelah memakai masker medis 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 1,10544 ng/ml dan setelah -2,04119 ng/ml. Hasil IgA kelompok eksperimen sebelum pemakaian masker herbal 24,00840 pg/ml dan setelah memakai masker herbal 29,74473 pg/ml. Sementara kadar IL6 sebelum adalah 0,91682 ng/ml dan setelah 0,500157 ng/ml. Berdasarkan hasil penelitian terdapat peningkatan kadar IgA setelah pemakaian masker medis dan masker herbal sementara kadar IL6 mengalami penurunan setelah pemakaian masker medis dan masker herbal. Keywords: Herbal mask; IgA; IL6, Masker herbal;IgA;IL6

THE EFFECTIVENESS OF HYDROLYZED VCO CREAM ON VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) EXPRESSION AND COLLAGEN THICKNESS FOR HEALING 2ND DEGREE BURNS INJURY IN WISTAR RATS Fahmi Syarif; Neni Susilaningsih; Yuriz Bakhtiar
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 3 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

ABSTRACT Burn wounds remain a serious problem in several countries. The presence of burn wounds might trigger local or in severe cases also triggers systemic response. Immediate treatment is required to prevent further tissue damage. Silver sulfadiazine cream is commonly used as a burn therapy. However, long-time usage might cause negative side effects. Several effective alternatives with better safety are opted, including virgin coconut oil (VCO). Originating from Cocos nucifera, commonly known as the coconut plant, VCO is a product that contains a lot of beneficial substances, such as lauric acid, polyphenol, and alpha-tocopherol. Hydrolyzed VCO can be an alternative to topical drugs in second-degree burns as it increases Vascular Endothelial Growth Factor (VEGF) expression and collagen thickness in the burn wound healing phase. This randomized post-test only with parallel-group experiment was done using Wistar rats induced to a second-degree burn. The effect of 70% and 100% on second-degree burn healing, measured by hydrolyzed VCO on VEGF expression and collagen thickness was observed between 6 and 12 days of therapy. VEGF expression and collagen expression increased higher in groups treated with 70% and 100% hydrolyzed VCO compared with the control group treated with base cream.

Effect of kelor (Moringa oleifera Lam) ethanolic leaves extract on uterus of pregnant mice and its teratogenicity Desy Armalina; Neni Susilaningsih; Indah Saraswati; Eva Annisa
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 53, No 4 (2021)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005304202102

Moringa oleifera Lam (Moringaceae) is a source of additional dietary. It contains 9 essential amino acids, calcium, iron, potassium, magnesium, zinc and vitamins A, C, E and B which have a major role in the immune system. Previous study reported that M. oleifera Lam can increase the hemoglobin of breastfeeding mothers. In contrast, M. oleifera Lam is well known as abortifacient plant, therefore it is not recommended to use during pregnancy. The aim of this study was to evaluate the effect of M. oleifera Lam ethanolic leaves extract M. oleifera Lam on uterus of pregnant mice and its teratogenic effect. Twenty pregnant female Balb/c mice were used in this experimental study. Mice were randomized divided into four groups with five mice in each group. Group C as control, mice were not given anything. Group T1-T3 were given M. oleifera Lam ethanolic leaves extract during pregnancy age 7-18 days with doses of 10, 20 and 30 mg/kg BW, respectively. Phytochemical analysis was performed quantitatively and qualitatively. On day 19, a caesarean section was performed to take the uterus, count the number of living foetus, dead foetus, disability, and other morphological abnormalities. No significant difference between all groups was observed. The abortifacient effect was not also observed in the uterus. Qualitative phytochemical analysis results showed that alkaloids, flavonoids, phenols, steroids, and tannins are identified in this extract. Quantitative steroids analysis results showed that the extract contained steroids of 1057.6 ppm. In conclusion, the M. oleifera Lam ethanolic leaves extract at dose of 30 mg/kg BW does not show any effect on the uterus of pregnant mice. Further study concerning the effects on other organs and hormones is recommended.

Phlebotrophic Effect of Graptophyllum Pictum (L.) Griff on Experimental Wistar Hemorrhoids Mario Sadar Bernitho Hutagalung; Bernadus Parish Budiono; Sigit Adi Prasetyo; Ignatius Riwanto; Eriawan Agung Nugroho; Yan Wisnu Prajoko; Neni Susilaningsih
Journal of Biomedicine and Translational Research Vol 5, No 1 (2019): July 2019
Publisher : Faculty of Medicine, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v5i1.3704

Background : The 1st and 2nd degree hemorrhoids is managed non-operatively with the anti-inflammatory and plebothropic drugs. Graptophyllum pictum extract (GPE) has already been used widely in Indonesia to treat hemorrhoid with good result, however, the mechanism is not supported by the molecular research.Objective : This study is intended to study the phlebothropic effects of GPE by measuring the degree of edema and extravassal leucocytes of experimental wistar hemorrhoid.Methods : Experimental study with Randomized Controlled Trial Post-test only design in male wistar rats, weight around 200 gr, induced for the development of a disease-like condition of hemorrhoids by 6% croton oil on the anus for 3 days. Random allocation was performed to divide the 14 wistar rats in 2 groups. Group I as control got normal saline solution, while group II was treated with GPE 100mg/kgbw, started on day 4th for 5 consecutive days. On 9th day blood was extracted from retroorbital fossa and anus was resected up to 2 cm from anal verge and weighted. The degree of anal edema was measured by rectoanal coefficient, that is rasio between anal weight (miligrams) and body weight (grams).Results : Until the end of study, all wistar rat were still alive. The mean (±SD) of body weight of control group was 173.84 (±13.37) and the treatment group was 171.70 (±13.10), and there was no significant differences (p = 0.833). The mean (±SD) of rectoanal coefficient in the treatment group was 2.46 (±0.41) and it was significantly lower than control group (3.13 ± 0.85) (p = 0.029). The mean (±SD) of extravassal leukocytes in the treatment group was 900.14(±48.09) and it was significantly lower than the control (1003.28 ± 99.30) (p = 0.042).Conclusions : Graptophyllum pictum extract at doses of 100 mg/Kgbw have phlebotonic effect in decreased edema and extravassal leukocytes.

Production of tumor necrosis factor-a is increased in urinary tract infections Neni Susilaningsih; Bambang Joni Karjono; Ratna Damma Purnamawati
Universa Medicina Vol. 31 No. 3 (2012)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2012.v31.167-174

BACKGROUND Urinary tract infection (UTI) is a common source of bacteriemia. The most common cause of UTI is Escherichia coli (E. coli). Tumor Necrosis Factor (TNF)-á gene polymorphism has been reported to be responsible for an excessive production of TNF-á and eventual disruption of pro-inflammatory cytokine regulation. The aim of this study was to compare TNF-á serum levels and TNF-á allele polymorphisms in patients with UTI due to E.coli and in non- UTI controls. METHODS A cross-sectional study was conducted at Dr. Kariadi Central Hospital and the Center for Biomedical Research, Faculty of Medicine, Diponegoro University, Semarang. In 68 patients with UTI the TNF-á serum levels were determined by means of ELISA and compared to those of non-UTI controls (n=55). TNFá- 308G>A gene polymorphism was analyzed by polymerase chain reaction restriction fragment length using the NcoI enzyme. Fragments were visualized on polyacrylamide gel with silver staining. RESULTS TNF-á serum level in patients with UTI had a median of 8.9 pg/mL, which was significantly higher than the median of 3.7 pg/mL in the control group (pA gene polymorphisms found in the patient group were G/G=61 (90%), G/A=7(10%) and A/A=0, while in the control group were G/G=48 (87%), G/A=7 (13%) and A/A =0. There was no significant differences (p=0.578) in gene polymorphisms between the two groups. CONCLUSIONS TNF-á serum levels in patients with UTI due to E. coli were significantly higher than in non-UTI controls, but for the TNF-á-380 gene polymorphisms no significant difference was found between the two groups. There are presumably more important factors than host genotype that influence UTI pathogenesis.

The Effect of Multilevel Doses of Caffeine on Tissue Macrophage and Blood Lymphocyte Count in Autologous Full Thickness Skin Graft Healing in Sprague Dawley Rats Wahyu Haris Prabowo; Awal Prasetyo; Neni Susilaningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 6 No. 5 (2022): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/bsm.v6i5.498

Backgrounds. A skin graft is one of the routine surgical procedures performed. This procedure gives excellent results when done as early as possible after the trauma. Caffeine has a mechanism as an adenosine-receptor A2 antagonist which can induce wound healing through increased angiogenesis. This study aimed to determine the efficacy of coffee caffeine in the initiation of full thickness skin graft autologous wound healing by assessing the number of macrophages and lymphocytes in Sprague-Dawley rats. Methods. The research design is an experimental study with a post-test-only approach with a control group design. Twenty male Sprague Dawley rats aged 15 weeks (140 – 150 grams) were randomly divided into four groups. One group became the control group (decaffeinated) while the other group received various doses of caffeine (3 mg/kgBW, 6 mg/kgBW, and 9 mg/kgBW). Autologous skin grafts were performed in all groups. HE examination was performed to confirm the number of macrophage cells in the tissue. Data analysis was carried out with the help of SPSS 25 software. Results. There were significant differences in the number of tissue macrophages in the four groups. The group that received 9 mg/kgBW of caffeine showed the highest number of macrophages compared to the other groups. Based on the number of lymphocytes in the peripheral blood, the group that received a dose of 6 mg/kg BW showed the highest number of lymphocytes compared to other groups. Conclusion. Caffeine showed the ability to initiate full thickness skin graft autologous wound healing by increasing the number of macrophages and lymphocytes in Sprague Dawley rats.

Co-Authors Abdul Hakam Abdul Mughni Abraham Simatupang Aditya Purnama Agnes Stella Valentina Akhmad Ismail Almaz, Ayyasi Izaz Ambariyanto , Ar, Ardiyana Ari Suwondo Ariyanto, Diaza O. Asri, Hairul Astika Widy Utomo, Astika Widy Awal Prasetyo Azhar, Abu Bambang Joni Karjono Bambang Witjahjo Bambang Witjahjo Bernadus Parish Budiono Budiono, Bernardus Parish Bulandari, Beatrice LA. Cahyono, Chemy Wiryawan Catharina Suharti Damma Purnawati, Ratna Desy Armalina Dimarti, Safira Chairani Dwi Marliyawati Elvira Yunita Endang Sri Lestari Eriawan Agung Nugroho Eriawan Agung Nugroho, Eriawan Agung Eva Annisa Fahmi Syarif Fanti Saktini Ferry Sandra Fidela Hanan Zivana Fifin Luthfia Rahmi Fuadi, Ahmad F. Hardhono Susanto Hardian Hardian Hari Peni Julianti Helmia Farida Hermawan Istiadi Iffiyana, Alifatul Ignatius Riwanto, Ignatius Indah Saraswati Joni Karjono, Bambang Kanti Yunika Kurniaty, Linggom Kusuma, Nauval Marta Mahabuana, Bintang Mahendra, Vito Maria Eka Patri Y Maria Mexitalia Mario Sadar Bernitho Hutagalung Meiny Suzery Mulyadi Djojosaputro, Mulyadi Muttaqien, Rizal Arief, Najatullah Najatullah Najatullah, Najatullah Nani Maharani Nugrahadi, Dimas Erlangga Nugroho, Trilaksana Parish Budiono Prabowo, Erik Pritadesya, Maharani Prizka Purnawati, Ratna Dama Purnomo, Hery D. Putri, Ajeng Kurniasari Rahajeng, Herera Rampengan, Derren DCH. Ratna Damma Purnamawati Ratna Damma Purnawati Renni Yuniati Respati, Danendra RK. Retno Murwani Ryco Giftyan Ardika Sigit Adi Prasetyo Sigit Adi Prasetyo, Sigit Adi Silaban, Hertina Sri Susilowati Suhartono, Suhartono Supandi Andy SyarofilAnam, Moh. Tanti Ajoe Kesoema, Tanti Ajoe Tjandra, Kevin C. Tobing, Romauli Tri Widyawati, Tri Trilaksana Nugroho Udadi Sadhana, Udadi Ulfa Nurullita Wahyu Haris Prabowo Widiastiti, Nyoman Suci Yan Wisnu Prajoko Yuriz Bakhtiar Zhafran Hafizhki

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