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All Journal Jurnal Ilmiah Samudra Akuatika SIGANUS: Journal of Fisheries and Marine Science
Darsiani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Immunology & microbiology

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Pengujian In-Vivo Ekstrak Rumput Laut Dalam Menangani Bakteri Pathogen Yang Menyerang Ikan Kerapu Yanti Mutalib; Lady Dian Khartiono; Admi Athirah; Darsiani; Teuku Fadlon Haser
Jurnal Ilmiah Samudra Akuatika Vol 3 No 1 (2019): Jurnal Ilmiah Samudra Akuatika
Publisher : Fakultas Pertanian Universitas Samudra

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (343.432 KB)

Abstract

Vibriosis controlling in the cultivation of grouper fishes still counts on the use of synthetic pharmacy or antibiotics. However, prolonged used of antibiotics can resulted in bacteria becoming resistance. Antibacterial agent derived from natural resources is expected to be able to overcome the problem. Ulva reticulata extract is one of the candidates that has been tested in-vitro to possessed antimicrobial bioactive compounds. This study aimed at testing its effectivity against pathogenic bacteria infecting Humpback grouper. Final results shows that the highest concentration (2000 ppm) given to the fish soaking tanks gives the best results.
Pengaruh Pemberian Antibiotik Terhadap Sintasan dan Pertumbuhan Eksplan Rumput Laut Kappaphycus alvarezii Secara In Vitro Bin Abd Jabbar, Firmansyah; Ardiansyah; Darsiani
SIGANUS: Journal of Fisheries and Marine Science Vol 2 No 1 (2020): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v2i1.818

Abstract

ABSTRACT: The present study aimed to determine the effect of antibiotic on the survival and growth performance of K. alvarezii using in vitro technique. This research was conducted for 2 months in Pangkep State of Agricultural Polytechnic at Tissue Culture Laboratory. The seaweed samples were taken from waters of Pangkep Regency. The fresh and healthy thallus branches were used as explants. The culture medium was conwy liquid media added vitamin mix with the treatment of different antibiotic concentrations. The parameters observed were survival rate, number of shoots, shoot length. A completely randomized design and 5 treatments with 3 replications were applied. One way ANOVA was performed for data analysis. The results showed that survival rate and shoot length and the highest number of shoots were found at 10 ppm antibiotic treatment with 56.6% of survival rate, the average number of shoots was 6, the average length of shoots was 1.86 mm. while a total 30% of survival rate was found at 12 ppm treatment with a number of shoots reaching 5.4 and a shoot length of 1.79. ABSTRAK:Tujuan penelitian ini adalah untuk mengetahui pengaruh perbedaan dosis antibiotik pada media kultur jaringan terhadap kelangsungan hidup dan pertumbuhan rumput laut K. alvarezii secara in vitro. Penelitian ini dilakukan selama 2 bulan di Laboratorium Kultur Jaringan Politeknik Pertanian Negeri Pangkep. Bahan uji yang digunakan adalah bibit rumput laut jenis Kappaphycus alvarezii yang diperoleh dari perairan Kabupaten Pangkep. Bibit yang digunakan adalah bibit segar, muda, memiliki percabangan yang banyak dan sehat. Media kultur adalah media cair conwy dengan campuran vitamin mix dengan perlakuan perbedaan konsentrasi antibiotik yang berbeda. Wadah percobaan adalah botol kultur dengan volume 200 ml sebanyak 15 buah. Parameter yang diamati sintasan, jumlah tunas, panjang tunas. Rancangan yang digunakan adalah Rancangan Acak lengkap (RAL) dan 5 perlakuan sebanyak 3 ulangan. One way ANOVA digunakan untuk analisis data. Hasil penelitian memperlihatkan bahwa sintasan dan pertumbuhan panjang tunas serta jumlah tunas tertinggi adalah pada perlakuan antibiotik 10 ppm. Sintasan eksplan rumput laut mencapai 56.6%, rata-rata jumlah tunas mencapai 6, rata-rata panjang tunas mencapai 1.86 mm. sedangkan perlakuan sintasan untuk perlakuan 12 ppm hanya 30%, dengan jumlah tunas mencapai 5.4 serta panjang tunas 1.79.
Respon Penetasan Telur Ikan Mas (Cyprinus carpio) pada Tingkatan Suhu yang Berbeda Muslim, Ilham; Atjo, Andi Arham; Darsiani
SIGANUS: Journal of Fisheries and Marine Science Vol 2 No 2 (2021): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v2i2.1017

Abstract

ABSTRACT: The present study aimed to determine the effect of different temperature levels on the hatching rate of common carp eggs. This study consisted of 12 experimental units, in which 4 treatments with 3 replications were applied, namely A (control temperature 22-29 ° C), B (24 ° C), C (temperature 28 ° C), and D (temperature 32 ° C). Temperature, pH, DO and hatching rate was measured for data collection. Analysis of variance (ANOVA) with a confidence level of 95% was performed for statistical analysis. Water quality data were descriptively described. The results showed that the highest number of hatching rate was obtained in treatment D (32 ° C) of 290 (99%), C (28 ° C) of 276 (94%), treatment B (24 ° C) of 251 individuals, and the lowest was observed in treatment A (22-29 ° C) of 242 individuals (83%). Statistically, the temperature of 22-32 ° C did not affect the hatching rate of common carp eggs. ABSTRAK: Penelitian ini bertujuan untuk mengetahui pengaruh tingkatan suhu yang berbeda terhadap daya tetas telur ikan mas. Penelitian ini terdiri dari 12 satuan percobaan, yang mana terdapat 4 perlakuan dengan 3 ulangan yakni A (kontrol suhu 22-29°C), B (24°C), C (suhu 28°C) dan D (suhu 32°C). Parameter uji meliputi suhu, pH, DO dan daya tetas telur (hatching rate). Data yang diperoleh dianalisis menggunakan analisis ragam (ANOVA) dengan tingkat kepercayaan 95% dan jika menunjukkan pengaruh nyata akan dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Data kualitas air dijelaskan secara deskriptif sesuai kelayakan hidup ikan mas. Hasil penelitian menunjukkan bahwa jumlah telur yang menetas terbanyak diperoleh pada perlakuan D (32°C) sebesar 290 ekor (99%), perlakuan C (28°C) sebesar 276 ekor (94%), perlakuan B (24°C) sebesar 251 ekor dan terendah pada perlakuan A (22-29°C) sebesar 242 ekor (83%). Hasil uji statistik menunjukkan bahwa suhu 22-32°C tidak berpengaruh terhadap daya tetas telur Ikan Mas.
Co-Authors Admi Athirah Ardiansyah Atjo, Andi Arham Jabbar, Firmansyah Bin Abd Lady Dian Khartiono Muslim, Ilham Teuku Fadlon Haser Yanti Mutalib