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Chemical Characterization of Moringa oleifera Lam. from Six Growth Locations in Central of Java: An Initiation of Standardization `: Karakterisasi Kimia Moringa oleifera Lam. dari Enam Lokasi Tumbuh di Jawa Tengah: Inisiasi Standardisasi Budiarti, Mery; Wijaya, Nur Rahmawati; Jokopriyambodo, Wahyu
Jurnal Tumbuhan Obat Indonesia Vol. 17 No. 1 (2024): July 2024
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v17i1.1192

Moringa oleifera Lam. is in high demand as a raw material for pharmaceutical constituents and dietary supplements, particularly for producing stunting supplements. However, recommendations regarding M. oleifera quality standards have not supported this claim as scientific evidence of its safety and effectiveness. This research intends to expand knowledge regarding the quality standards of M. oleifera leaves, particularly their chemical properties. Height-variant samples of M. oleifera were collected from six locations in the Central Java, Indonesia. A hierarchical cluster analysis (HCA) was performed to group the most each parameter's values. The parameters included water-soluble extract, ethanol-soluble extract, total ash content, acid-insoluble ash, total flavonoid content was quantified as quercetin, and thin-layer chromatography (TLC) fingerprint. The suggestion of specific value for each parameter as a future reference for M. oleifera, including water- and ethanol-soluble extract content of at least 41% and 19%, and a total- and acid-insoluble ash content of a maximum of 11% and 4%, respectively. The minimal quercetin contains as the chemical marker compound of M. oleifera was a minimum of 1.285%. The chromatogram profile of TLC suggested 7 (254 nm) and 8 (366 nm) spots.

Karakterisasi Minyak Atsiri dari Simplisia Basah Ranting dan Daun sebagai Alternatif Subtitusi Kulit Batang Cinnamomum burmannii Blume Budiarti, Mery; Jokopriambodo, Wahyu; Isnawati, Ani
Jurnal Kefarmasian Indonesia VOLUME 8, NOMOR 2, AGUSTUS 2018
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v8i2.323

The bark of Cinnamomum burmannii Blume is the main raw material of Cinnamomum oil because its cinnamaldehyde content is higher than in the other parts. This condition lead to the exploitation of Cinnamomum burmannii Blume bark without any cultivation effort, thus it can cause the raw material scarcity. Twigs and leaves of Cinnamomum burmannii Blume are also known to contain cinnamaldehyde therefore it provide a potential alternative source of cinnamaldehyde. The purpose of this research is to investigate the characteristic of Cinnamomum burmannii Blume essential oil which includes refractive index, profiling of chemical constituent and percentage of cinnamaldehyde as a marker compound. This research used three fresh Cinnamomum burmannii Blume simplicia of bark, twigs and leaves from two selected growing locations: Tawangmangu and Purwokerto. Simple water distillation method was conducted, together with characterization and refractive index using refractometer, followed by qualitative and quantitative analysis with Thin Layer Chromatography (TLC) and Gas Chromatography (GC). The results showed Cinnamomum burmannii Blume oil derived from bark, twig and leaf have different profiles, but all have cinnamaldehyde as the main component. The leaf produces higher essential oil yield percentage compared to the twig with a cinnamaldehyde content that is comparable to the bark by 50% of cinnamaldehyde content in cinnamomum bark oil.

Autentikasi Centella asiatica (L.) Urb. (Pegagan) dan Adulterannya Berdasarkan Karakter Makroskopis, Mikroskopis, dan Profil Kimia Maruzy, Anshary; Budiarti, Mery; Subositi, Dyah
Jurnal Kefarmasian Indonesia VOLUME 10, NOMOR 1, FEBRUARI 2020
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v10i1.1830

Pegagan or Centella asiatica (L.) Urb. empirically used as medicinal plant and scientifically can be used as antihypertension based on the results of the Jamu Saintification. Raw materials from C. asiatica have the potential to occur adulteration or incorrect use of plants species. There are two species of plants that have the potential to become adulterants of C. asiatica, namely Hydrocotyle verticillata and Merremia emarginata. Thus, the purpose of this study is to authenticate from Pegagan by comparing the macroscopic, microscopic, and chemical profiles of C. asiatica, H. verticillata, and M. emarginata. The method of data collection is done by purposive sampling using form intruments to record macroscopic and microscopic characters, while the chemical profiles are tested using thin layer chromatography methods to determine the content of asiaticosides in both potential adulterants species. The research was conducted at three sampling points: Central Java (Tawangmangu), West Java (Bogor), and East Java (Malang), while for laboratory research activities carried out at B2P2TOOT, Tawangmangu. The results showed macroscopically unique and easy distinguishing characters between C. asiatica and potential adulterant species H. verticillata is a leaf shape, whereas C. asiatica with M. emarginata are leaf-type appearance characters. The character of the simplicia leaf shape between C. asiatica and H. verticillata can be a distinct differentiator characters. Anatomical characters and powder fragments that can be used as a differentiator characters between C. asiatica and H. verticillata are the presence of pith on the stalk and stolon, the type of stomata, and the presence of trichomes. Authentication of simplicia powder of H. verticillata as an adulterant potential of C. asiatica with TLC, can be easily known by the absence of asiaticosides in these simplicia so that H. verticillata cannot be a substitute species for C. asiatica

Chemical Characterization of Moringa oleifera Lam. from Six Growth Locations in Central of Java: An Initiation of Standardization `: Karakterisasi Kimia Moringa oleifera Lam. dari Enam Lokasi Tumbuh di Jawa Tengah: Inisiasi Standardisasi Budiarti, Mery; Wijaya, Nur Rahmawati; Jokopriyambodo, Wahyu
Jurnal Tumbuhan Obat Indonesia Vol. 17 No. 1 (2024): July 2024
Publisher : Universitas Tidar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31002/jtoi.v17i1.1192

Moringa oleifera Lam. is in high demand as a raw material for pharmaceutical constituents and dietary supplements, particularly for producing stunting supplements. However, recommendations regarding M. oleifera quality standards have not supported this claim as scientific evidence of its safety and effectiveness. This research intends to expand knowledge regarding the quality standards of M. oleifera leaves, particularly their chemical properties. Height-variant samples of M. oleifera were collected from six locations in the Central Java, Indonesia. A hierarchical cluster analysis (HCA) was performed to group the most each parameter's values. The parameters included water-soluble extract, ethanol-soluble extract, total ash content, acid-insoluble ash, total flavonoid content was quantified as quercetin, and thin-layer chromatography (TLC) fingerprint. The suggestion of specific value for each parameter as a future reference for M. oleifera, including water- and ethanol-soluble extract content of at least 41% and 19%, and a total- and acid-insoluble ash content of a maximum of 11% and 4%, respectively. The minimal quercetin contains as the chemical marker compound of M. oleifera was a minimum of 1.285%. The chromatogram profile of TLC suggested 7 (254 nm) and 8 (366 nm) spots.

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