Article Per Year (5 Year)
p-Index From 2021 - 2026
0.659
P-Index
This Author published in this journals
All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Journal of Tropical Biodiversity and Biotechnology Jurnal Biodjati Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Jurnal Kefarmasian Indonesia
Maruzy, Anshary
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology

Published : 5 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 5 Documents
Search

 1 
Intraspecific Variability and Phenetic Relationships of Centella Asiatica (L.) Urb. Accessions from Central Java Based on Morphological Characters’ Maruzy, Anshary; Susandarini, Ratna
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 1 (2024): March
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.86477

Abstract

Centella asiatica (L.) Urb. is a plant species native to Java and one of the main basic materials in traditional and modern medicine. This study is the first to report the intraspecific variation and taxonomic relationships of C. asiatica accession from natural populations in Central Java.  The purpose of this study was to reveal phenotypic variations of C. asiatica populations and to asses phenetic relationships based on morphological characters. Thirty-two accessions of C. asiatica were collected from natural populations from eight mountains in Central Java. Observation on vegetative organs resulted in 25 morphological characters as a basis for assessing phenetic relationships using cluster analysis and principal component analysis. Result of cluster analysis showed that the grouping of accessions was not correlated to the localities from where the samples were collected, although there was a tendency that accessions from the same localities grouped in one cluster. The results of this study confirmed the existence of intraspecies morphological variability in C. asiatica which was not affected by geographical aspects. Results of principal component analysis indicated that the grouping of accessions was mainly determined by similarities in petiole color, stolon color, leaf margin, petiole length, stolon length, and leaf color. Given that the characters contributing to the grouping of accessions were mainly qualitative characters, the results indicated a genetic basis underlying phenotypic variations of C. asiatica accession.  
Autentikasi Centella asiatica (L.) Urb. (Pegagan) dan Adulterannya Berdasarkan Karakter Makroskopis, Mikroskopis, dan Profil Kimia Maruzy, Anshary; Budiarti, Mery; Subositi, Dyah
Jurnal Kefarmasian Indonesia VOLUME 10, NOMOR 1, FEBRUARI 2020
Publisher : Pusat Penelitian dan Pengembangan Biomedis dan Teknologi Dasar Kesehatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/jki.v10i1.1830

Abstract

Pegagan or Centella asiatica (L.) Urb. empirically used as medicinal plant and scientifically can be used as antihypertension based on the results of the Jamu Saintification. Raw materials from C. asiatica have the potential to occur adulteration or incorrect use of plants species. There are two species of plants that have the potential to become adulterants of C. asiatica, namely Hydrocotyle verticillata and Merremia emarginata. Thus, the purpose of this study is to authenticate from Pegagan by comparing the macroscopic, microscopic, and chemical profiles of C. asiatica, H. verticillata, and M. emarginata. The method of data collection is done by purposive sampling using form intruments to record macroscopic and microscopic characters, while the chemical profiles are tested using thin layer chromatography methods to determine the content of asiaticosides in both potential adulterants species. The research was conducted at three sampling points: Central Java (Tawangmangu), West Java (Bogor), and East Java (Malang), while for laboratory research activities carried out at B2P2TOOT, Tawangmangu. The results showed macroscopically unique and easy distinguishing characters between C. asiatica and potential adulterant species H. verticillata is a leaf shape, whereas C. asiatica with M. emarginata are leaf-type appearance characters. The character of the simplicia leaf shape between C. asiatica and H. verticillata can be a distinct differentiator characters. Anatomical characters and powder fragments that can be used as a differentiator characters between C. asiatica and H. verticillata are the presence of pith on the stalk and stolon, the type of stomata, and the presence of trichomes. Authentication of simplicia powder of H. verticillata as an adulterant potential of C. asiatica with TLC, can be easily known by the absence of asiaticosides in these simplicia so that H. verticillata cannot be a substitute species for C. asiatica
Seleksi Primer RAPD untuk Autentikasi Kumis Kucing (Orthosiphon aristatus (Blume) Miq.) Subositi, Dyah; Maruzy, Anshary; Wijaya, Nur Rahmawati
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2020: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (436.8 KB)

Abstract

Kumis kucing (Orthosiphon aristatus) merupakan tumbuhan yang dimanfaatkan sebagai bahan baku obat tradisional oleh masyarakat umum maupun industri. Tanaman ini berpotensi dipalsukan atau terjadi kekeliruan dengan tekelan (Eupatorium riparium) karena mempunyai morfologi daun yang mirip terutama dalam bentuk simplisia. Penggunaan karakter morfologidan fitokimia dalam autentikasi tanaman obat mempunyai keterbatasan karena dipengaruhi oleh faktor lingkungan dan umur fisiologis tanaman. Penanda molekular RAPD merupakan salah satu metode autentikasi tumbuhan obat yang tidak dipengaruhi oleh kedua faktor tersebut. Tujuan dari penelitian ini adalah untuk seleksi primer RAPD sebagai tahap awal dalam autentikasi kumis kucing. Isolasi DNA genom dilakukan pada kumis kucing, tekelan dan campuran keduanya, selanjutnya dilakukan amplifikasi menggunakan 20 primer RAPD. Tiga primer RAPD tidak dapat menghasilkan produk amplifikasi dan total fragmen yang dihasilkan sebanyak 97 fragmen DNA, serta polimorfisme sebesar 43,3%. Sebanyak 3 primer RAPD yaitu OPK2, OPL-1 dan OPM1menghasilkanmasingmasingsatufragmen spesifik pada kumis kucing dan tekelan. Primer RAPD tersebut dapat digunakan untuk autentikasi kumis kucing dengan tekelan.
Genetic Diversity of Graptophyllum pictum (L.) Griff from Eastern Indonesia Ethnics Based on Sequence-Related Amplified Polymorphismr Azmi, Wihda Aisarul; Husnawati; Puspita, Puspa Julistia; Safira, Ukhradiya M; Subositi, Dyah; Maruzy, Anshary
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 50 No. 2 (2022): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (638.903 KB) | DOI: 10.24831/jai.v50i2.40644

Abstract

Indonesia berada diposisi kedua dengan jumlah tanaman obat asli tertinggi di dunia setelah Hutan Hujan Amazon. G. pictum (L) Griff merupakan salah satu etnomedisin yang telah dimanfaatkan secara luas oleh masyarakat Indonesia, tapi keragaman genetiknya belum dipetakan. Penelitian ini bertujuan menganalisis keragaman genetik G. pictum (L) Griff dari Indonesia Bagian Timur berdasarkan marka molekuler SRAP melalui pencarian keberadaan polimorfisme. G. pictum (L) Griff yang digunakan berasal dari 11 etnis Indonesia Timur yang sudah dikeringkan sebelumnya, kemudian dilakukan isolasi DNA kromosom, amplifikasi PCR dengan menggunakan 8 kombinasi primer SRAP terpilih, kemudian dianalisis kekerabatan dari 7 etnis menggunakan software NTSYS (Numerical Taxonomy and Multivariative Analysis System) 2.0 dan PopGene (Population Genetic Analysis) 1.3. Dari hasil penelitian diperoleh nilai Persen Lokus Polimorfik (PLP) sebesar 88.55% dengan total lokus ditemukan 49 (44 lokus poliformik dan 5 lokus monomorfik), dan variasi Indonesia menduduki peringkat kedua dengan jumlah tanaman obat asli tertinggi di dunia setelah Hutan Hujan Amazon. Handeuleum (G. pictum (L) Griff) merupakan salah satu tanaman obat yang telah dimanfaatkan secara luas oleh masyarakat Indonesia, namun informasi mengenai keragaman genetiknya masih terbatas. Penelitian ini bertujuan untuk memperoleh informasi mengenai keragaman genetik 52 aksesi G. pictum (L) Griff dari 11 etnis Indonesia Bagian Timur berdasarkan marka molekuler SRAP. Penelitian ini dilaksanakan pada bulan November 2018 hingga Januari 2019. Sebanyak 52 aksesi G. pictum (L) Griff dan 16 kombinasi primer SRAP digunakan dalam penelitian ini. Analisis kekerabatan dilakukan berdasarkan nilai indeks similaritas dan konstruksi dendogram dengan menggunakan program NTSYS 2.0 dan PopGene 1.3. Sebanyak 49 lokus (44 lokus polimorfik dan 5 lokus monomorfik) diperoleh dari delapan kombinasi primer terpilih dengan presentase polimorfik sebesar 88.55%. Keragaman genetik antar aksesi tergolong tinggi (86%) sedangkan antar etnis tergolong rendah (47.23%). Etnis dengan keragaman tertinggi adalah Demta (81.63%), sedangkan Auyu adalah Etnis dengan keragaman genetik yang paling rendah (2.04%). Berdasarkan nilai Indeks Dissimilarity (ID) atau nilai keragaman yang menunjukkan tingkat kekerabatan, Etnis Bajawa dan Auyu memiliki tingkat kekerabatan terjauh (ID = 1.0720). Informasi yang dihasilkan dalam penelitian ini bermanfaat untuk mendukung program pemuliaan dan konservasi daun wungu. Kata kunci: dendogram, handeuleum, SRAP, tanaman obat
Genetic Profiling of Sida rhombifolia Originated from Several Indonesian Ethnicities Based on Sequence-Related Amplified Polymorphism Markers Solihah, Jumailatus; Kurniatanty, Isma; Subositi, Dyah; Maruzy, Anshary; Martiwi, Ika Nugraheny Ari; Ainy, Erny Qurrotul; Anam, Khoirul; Dina, Aslikh Lana
Jurnal Biodjati Vol 6 No 2 (2021): November
Publisher : UIN Sunan Gunung Djati Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15575/biodjati.v6i2.14553

Abstract

Sida rhombifolia is one of wild flowering plants that grows easily in many habitats with moderate humidity, with some usefulness in traditional medicine. Genetic characterization of Sida rhombifolia accessions originated from 12 ethnicities of Indonesia was analyzed based on Sequence-Related Amplified Polymorphism (SRAP) Markers. The genomic DNA were extracted from leaf samples and then were characterized by using the SRAP marker system according to Li and Quiros (2001). Nine pairs of SRAP primer resulted high polymorphic bands and were used in the genetic profiling. The data analysis was performed using GenAlEx to calculate genetic distance, Principal coordinate analysis, and Analysis of Molecular Variance (AMOVA), also using POPGENE to assess genetic diversity (Hs and Ht) and Nm to predict gene flow among populations. The coordinate analysis showed that the accessions originated from ethnicities along Wallacean line tend to differ genetically from most other locations. However, the results of analysis of molecular variance suggested that there were only slight differences (0.1%) found between ethnicities, while most genetic variances (99.9%) were found mostly among accessions within populations. The results suggested that there was an extensive genetic flow and plant spreading among Sida rhombifolia plant populations, resulting more homogenous genetic characters among most populations, while high diversity within population. The calculation of the number of migration (Nm = 1.7341) confirmed that the high rate of gene flow had occurred between populations.
Co-Authors Ainy, Erny Qurrotul Azmi, Wihda Aisarul Budiarti, Mery Dina, Aslikh Lana Dyah Subositi Husnawati KHOIRUL ANAM Kurniatanty, Isma Martiwi, Ika Nugraheny Ari Puspa Julistia Puspita Ratna Susandarini Safira, Ukhradiya M Solihah, Jumailatus Wijaya, Nur Rahmawati