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All Journal Jurnal Ilmu Pertanian Indonesia Acta Aquatica: Aquatic Sciences Journal Jurnal Pembelajaran dan Biologi Nukleus Indigenous Biologi Jurnal Penelitian Kehutanan Wallacea
Rafael, Andriani
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Other

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Diversity of natural dye plants in The Taplel Waterfall Area, Sisimeni Sanam Training Forest, Takari District, Kupang District Sabuna, Alan; Hendrik, Arnold Christian; Rafael, Andriani; Nge, Sonya Titin; Ngginak, James
Jurnal Penelitian Kehutanan Wallacea Vol. 12 No. 2 (2023)
Publisher : Foresty Faculty of Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/jpkwallacea.v12i2.27966

Abstract

The Taplel waterfall area which is in the Sisimeni Sanam Education and Training Forest area is one of the conservation areas in East Nusa Tenggara which has quite high biodiversity, including plants that produce natural colors. However, information on the species diversity of dye plants in the Taplel waterfall area is still lacking. The objective of this study was to uncover the diversity of natural dye plants in the Taplel waterfall area, Sisimeni Sanam Training Forest, Takari District, Kupang Regency. Data collection was carried out by setting sample plots measuring 2 x 2 meters (seedlings), 5 x 5 meters (saplings), 10 x 10 meters (poles), and 20 x 20 meters (trees). Analysis of the importance of species using the Importance Value Index, and species diversity using the Species Diversity Index (Shannon-Wiener Index). Species of natural dye plants found in the Taplel Waterfall Area include Tectona grandis, Cassia siamea, Acacia auriculiformis, Tamarindus indica, Ficus racemosa, Leucaena leucocephala, Schleichera oleosa, Bauhinia purpurea, Cassia fistula, Garuga floribunda, and Annona squamosa L.. Plant species that has the highest important role in the Taplel Waterfall Area is Cassia fistula (sapling), Bauhinia purpurea (pole), and Tectona grandis (tree). Meanwhile, the diversity of natural dye plant species in the Taplel Waterfall Area at the level of seedlings, saplings, poles and trees is in the moderate category.
Isolation and Selection of Extracellular Enzymes in Sponge Symbiont Bacteria (Porifera: Demospongiae) from Tablolong Beach Dima, Lintang A. M.; Rafael, Andriani; Nge, Sonya T.M; Radjasa, Ocky K.; Manalu, Tiodor S. J.; Ngginak, James
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.5222

Abstract

Marine biota has many benefits for human life. Sponges are a species of marine biota that can be used as a producer of antimicrobial compounds. The bacteria found in sponges have an important role in the continuity of life in the sea. The symbiotic lifestyle that occurs in bacteria and sponges has the opportunity to form substitutions for the content of secondary metabolites and enzymes, especially extracellular enzymes (amylase, protease, cellulose and lipase). This study aims to determine how to isolate sponge symbiotic bacteria and identify spongy symbiotic bacteria. The method used is purposive to take sponges. Characterization of bacteria was carried out based on morphology and gram staining. Enzymatic bacterial selection was carried out by testing the activity of the amylase enzyme (soluble starch), lipase enzyme (Teen 80), protease enzyme (skim milk) and cellulose enzyme (carboxyl methyl cellulose). Data analysis was performed in a qualitative descriptive manner by measuring the clear zone in the extracellular enzyme test results. Meanwhile, other research parameters measured in this study were temperature, salinity and pH. The results showed that out of 47 isolated bacteria and 33 of them had extracellular enzymes with 10 bacteria had amylase enzymes, 27 bacteria had lipase enzymes, 2 bacteria had protease enzymes and 1 isolate had cellulose enzymes.
Identification of endophytic fungi on the leaves of Rhyzophora stylosa Griff mangrove in the coastal area of Bokonusan Semau Village Rafael, Andriani; Sabuna, Alan Charis; Manifani, Derven
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 10, No 1: Jurnal Pembelajaran Dan Biologi Nukleus March 2024
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v10i1.5454

Abstract

Rhyzophora stylosa Griff is a mangrove plant that grows on the coast of Bokunusan village, Semau District, Kupang Region. Endophyte fungi are found in plant tissues and can function as agents for controlling pathogenic pests. The purpose of this research was to isolate and identify   endophyte fungi found in the young and mature leaf of R. stylosa Griff at the Mangrove forest, Bokunusan Village, Semau District, East Nusa Tenggara. This research was conducted from November to December 2023 at the Microbiology Laboratory; Biology Study Program, Aretha Wacana Christian University. The method used was the Potato Dextrose Agar (PDA) media and fungal identification based on macroscopic and microscopic characteristics. Based on the results of the study, nine isolate was isolated from young and mature leaf of R. stylosa Griff.   Two endophyte fungi were obtained from R. stylosa Griff leaves. The endophyte fungi found were genus Aspergillus and Trichoderma. 
Gastropods as Bioindicators of Water Quality in Telindale Beach, Rote Tengah District, Rote Ndao Regency Rafael, Andriani; Daud, Yanti; Manu, Theodora Sarlotha Nirmala; Gadi, Dewi Setiyowanto
Jurnal Ilmu Pertanian Indonesia Vol. 30 No. 2 (2025): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.30.2.320

Abstract

Telindale Beach is part of the Central Rote Sub-district in the Rote Ndao Regency. The beach is also a seaside region with many community activities, including tourism, recreation, and sales. These activities have a significant impact on the health of aquatic ecosystems. The presence of pollutants in water might cause a decline in its quality. Gastropods are among the species that are sensitive to changes in water quality. This study sought to provide information on gastropod diversity as a bioindicator of water quality at Telindale Beach. This study was both descriptive and quantitative, with the location determined through purposive sampling. The line-transect approach was applied at three locations, each with a different substrate. The data were evaluated using diversity, uniformity, and dominance index techniques. The research revealed that the gastropods identified at the beach belonged to 13 families, 15 genera, and 19 species, with 112 individuals across all stations. The diversity index ranged from 0.27 to 2.33 (moderately contaminated), the uniformity index was 0.84-0.91 (high), and the dominance index was 0.12-0.31 (moderate). According to the species diversity index data, the waters near Telindale Beach were the most polluted. Keywords: bioindicator, gastropods, Telindale Beach
KEANEKARAGAMAN MAKROINVERTEBRATA DI EKOSISTEM MANGROVE OESAPA BARAT KECAMATAN KELAPA LIMA Pinto, Roslin; Rafael, Andriani; Ngginak, James
INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI Vol 7 No 2 (2024): INDIGENOUS BIOLOGI : JURNAL PENDIDIKAN DAN SAINS BIOLOGI
Publisher : Prodi Pendidikan Biologi FKIP Universitas Kristen Artha Wacana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33323/indigenous.v7i2.654

Abstract

Makroinvertebrata merupakan hewan tidak bertulang belakang yang hidup melekat pada substrat. Jenis organisme ini berperan sebagai bioindikator kesehatan lingkungan. Tujuan dari penelitian ini adalah untuk menginventarisasi jenis-jenis makroinvertebrata yang terdapat di area ekosistem mangrove Oesapa Barat. Metode yang digunakan dalam penelitian ini adalah metode line transect. Teknik analisa data menggunakan analisa deskriptif kuantitatif. Berdasarkan hasil penelitian di area ekosistem mangrove Oesapa Barat ditemukan 17 spesies makroinvertebrata yang di kelompokkan dalam 4 kelas yaitu: Gastropoda, Bivalvia, Crustacea, dan Polychaeta. Indeks keanekaragaman spesies tertinggi terdapat pada transek II yaitu 1,68 sedangkan kepadatan, kepadatan relatif dan indeks dominansi tertinggi terdapat pada spesies C. Cobelti yaitu 9,67 ind/m2, 38,33% dan 0,14. Indeks keanekaragaman terendah terdapat pada transek III yaitu 1,22. Kepadatan, kepadatan relatif dan indeks dominansi ditemukan pada C. urceus, C. oulanense, Onchidium sp dan Uca sp yaitu 1,11 ind/m2, 0,44%. Indeks keanekaragaman di area ekosistem mangrove Oesapa Barat tergolong sedang. Salah satu bentuk usaha untuk melestarikan makroinvertebrata di lokasi penelitian adalah melakukan monitoring dengan pemantauan rutin polulasi makroinvertebrata serta melakukan penelitian lanjutan yang berkaitan dengan pola reproduksi atau siklus perkembangbiakan makroinvertebrata.
Phtytochemical screening and thin layer choromatography (TLC) profiling of mangrove family Rhizophoraceae and Avicenniaceae Rafael, Andriani
Acta Aquatica: Aquatic Sciences Journal Acta Aquatica: Jurnal Ilmu Perairan, Vol. 8: No. 1 (April 2021)
Publisher : Universitas Malikussaleh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29103/aa.v8i1.2461

Abstract

 Abstrak Penelitian ini adalah dari melaksanakan Februari hingga April 2017. menyalak dari tujuh spesies bakau yaitu ,. Ceriops decandra (Griff.) Ding Hou;  Ceriops tagal ( Perr.) CB Rob .; dan Bruguiera cylindrica ; Bruguiera gymnorrhiza (kiri) Lam; Rhizophora stylosa Griff .; Rhizophora mucronata Lam .; dan Avicennia marina (Forsk.) Vierh. Analisis fitokimia dan profil KLT dari ekstrak kasar kulit kayu yang dibandingkan. Analisis kualitatif menunjukkan bahwa Gonggongan Ceriops decandra (Griff.) Ding Hou, Ceriops tagal ( Perr.) CB Rob.,Bruguiera cylindrica, Bru guiera gymnorrhiza ( Kiri ) Lam, Rhizophora stylosa Griff. , Rhizophora mucronata Lam ., Dan Avicennia marina (Forsk.) Vierh. memiliki beberapa metabolit sekunder yang penting, yaitu senyawa Fenol, Tanin, dan Flavonoid. Flavonoid hanya ada di Avicennia marina (Forsk.) Vierh. Profil KLT menunjukkan bahwa dibandingkan DENGAN Nilai R f standar , Terdapat doa Titik hati kromatogram Ekstrak kasar  Rhizophora stylosa Griff. sama dengan nilai standar (nilai RfFlavonoid-glikosida turun antara = 0,18; 0,26; 0,30) menunjukkan bahwa Rhizophora stylosa Griff mungkin mengandung Flavonoid-glikosida dan asam fenolat ( nilai R f asam fenolat, 0,63-0, 84). Sedangkan Avicennia marina (Forsk.) Vierh mungkin mengandung asam fenolat. Namun, masih ada senyawa yang belum diketahui yang perlu diidentifikasi dalam penelitian lebih lanjut. Kata kunci: mangrove, fitokimia, Kromatografi Lapis Tipis 
Co-Authors Daud, Yanti Dima, Lintang A. M. Gadi, Dewi Setiyowanto Hendrik, Arnold Christian James Ngginak Manalu, Tiodor S. J. Manifani, Derven Manu, Theodora Sarlotha Nirmala Ocky K. Radjasa Pinto, Roslin Sabuna, Alan Sabuna, Alan Ch. Sonya Titin Nge