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All Journal Rumphius Pattimura Biological Journal Kalwedo Sains (KASA)
Pentury, Kresyan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology

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DYNAMICS COMMUNITIES OF BACTERIA AND YEAST IN PROCESSING ROBUSTA COFFEE WITH WET PROCESSING: SCREENING OF DOMINANT ISOLATES Nusaly, Windy Natalia; Pentury, Kresyan
RUMPHIUS Vol 2 No 2 (2020): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv2i2p043-049

Abstract

The type of coffee processing has a direct impact on the microbial community it contains and the quality of the coffee it produces. Coffee processing with a wet process (wet) is known to produce coffee with a good aroma and taste profile. This study aims to determine the dynamics of the bacterial and yeast community during the postharvest process and to determine the dominant microbes involved in the processing of Robusta coffee using a wet process. The method used was the isolation of bacteria and yeast using selective medium with the addition of antibiotics, then morphological identification and calculation of the index of diversity (H), dominance (D) and evenness (E) were carried out based on the bacterial and yeast isolates obtained, as well as screening of dominant isolates based on results of dominance analysis and enzymatic activity. The isolation results obtained by the yeast community (51.7%) with a higher number than the bacterial community (48.3%). The microbial community in the wet processing of Robusta coffee had a species diversity index (H') = 2.48, evenness (E) = 0.74 and dominance (D) = 0.17. The results of isolates screening through enzymatic tests showed bacterial isolates B10 and yeast Y2 were selected as dominant isolates and could be used as starter cultures in a controlled fermentation process.
Efek Antihiperkolesterolemia Seduhan Daun Cengkeh (Syzygium aromaticum L.) Terhadap Kolesterol Darah Tikus Model Siauta, Doviana; Pentury, Kresyan
Kalwedo Sains (KASA) Vol 2 No 1 (2021): Kalwedo Sains (KASA), Maret 2021
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav2i1p48-52

Abstract

Kadar kolestrol yang tinggi dalam darah dapat memicu munculnya berbagai penyakit sehingga perlu dilakukan treatmen penurunan kolesterol darah dengan menggunakan obat-obatan medis maupun herbal tubuhnya. Salah satu tanaman herbal yang diduga mampu menurunkan kadar kolesterol darah adalah tanaman cengkeh karena mengandung senyawa fitokimia seperti minyak atsiri, flavonoid, tannin, saponin, eugenol, polifenol. Penelitian ini bertujuan untuk mengkaji efek seduhan daun cengkeh (Syzygium aromaticum L.). terhadap penurunan kolesterol darah. Penelitian ini menggunakan Tikus Rattus norvegicus sebanyak 12 ekor yang dibagi dalam 4 kelompok perlakuan yaitu kelompok kontrol negatif, kelompok kontrol positif yaitu kelompok diberi lemak 2g/ekor/hari selama 7 hari, kelompok 0.09 yaitu kelompok yang diberi lemak 2g/ekor/hari selama 7 hari kemudian diberi seduhan daun cengkeh 0.09g/ekor/hari selama 14 hari dan kelompok 0.18 yaitu kelompok yang diberi lemak 2g/ekor/hari selama 7 hari kemudian diberi seduhan daun cengkeh 0.09g/ekor/hari selama 14 hari. Tiap perlakuan diulang sebanyak 3 kali. Variabel yang diamati adalah kadar kolesterol darah. Data yang didapat, dianalisis menggunakan ANOVA. Hasil penelitian menunjukkan bahwa pemberian seduhan daun cengkeh 0.09g/ekor/hari belum mampu menurunkan kadar kolesterol sedangkan 0.18g/ekor/hari selama 14 hari mampu menurunkan kolesterol darah tikus.
Dinamika dan Ekobiologi Teripang di Perairan Desa Hunuth, Kota Ambon Huwae, Laury M. Ch.; Hukubun, Ronald D.; Wakano, Deli; Silahooy, Veince B.; Pentury, Kresyan
Kalwedo Sains (KASA) Vol 2 No 2 (2021): Kalwedo Sains (KASA), September 2021
Publisher : Program Studi Di Luar Kampus Utama Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/kasav2i2p106-112

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh parameter fisik kimia perairan terhadap pertumbuhan teripang, menginventarisir jenis-jenis teripang, dan tingkat eksploitasi teripang. Penelitian dilakukan di perairan pesisir Desa Hunut, Kota Ambon pada bulan Desember 2020. Hasil penelitian menunjukan bahwa kondisi perairan sangat mendukung keberadaan dan pertumbuhan teripang. Didapati pula bahwa teripang di perairan pesisir Desa Hunut didominasi oleh famili Holothuriidae dan famili Stichopodidae. Aktifitas penangkapan yang tidak memperhatikan aspek keberlanjutan akan mengancam keberadaan sumberdaya teripang.
Co-Authors LAURY CHARA HUWAE Nusaly, Windy Natalia Ronald Darlly Hukubun Siauta, Doviana Silahooy, Veince B. Wakano, Deli