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All Journal Jurnal Biologi Tropis Jurnal Analis Kesehatan Jurnal Kesehatan KESMAS UWIGAMA: Jurnal Kesehatan Masyarakat Jurnal Farmasi Journal of Medical Laboratory in Infectious and Degenerative Diseases Jurnal Pengabdian dan Pemberdayaan Kesehatan
Saktiningsih, Hari
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Effect of Monoreagent Delay Time Variation at Room Temperature on Creatinine Levels Hidayah, Layla Nur; Saktiningsih, Hari
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11265

Abstract

Creatinine is a waste product of body metabolism that is excreted by glomerular filtration in the kidneys. One method for measuring serum creatinine is the Jaffe Reaction which is based on the reaction between creatinine and picric acid in an alkaline medium, forming a red-orange complex. Creatinine reagents have a shorter shelf life or stability, especially after being mixed into a monoreagent. Monoreagents that are delayed may affect the levels of the analyte being tested. Based on this, the study aims to determine the effect of variations in monoreagent delay time at room temperature on creatinine levels. This research used an analytical experimental design and was conducted in the STIKES Nasional laboratory. A total of 10 samples were obtained using quota sampling from students of class 2A2, D-III Medical Laboratory Technology, STIKES Nasional Surakarta. A total of 30 data points were analyzed using the non-parametric Friedman test. The results showed that the average creatinine levels at 0 minutes, 3 hours, and 4 hours were 0.611 mg/dL, 0.555 mg/dL, and 0.572 mg/dL, respectively. Normality testing indicated the data were not normally distributed, so that the non-parametric Friedman test was used. The test results showed a p-value of 0.014 (p < 0.05), indicating a statistically significant difference. In conclusion, variation in monoreagent delay time at room temperature affects creatinine levels.
The Differences SGPT Levels Using Immediately Monoreagent And Delayed For 5 Days At Room Temperature Putri, Salma Yuki Anisa; Saktiningsih, Hari
Jurnal Biologi Tropis Vol. 26 No. 1 (2026): Januari-Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v26i1.11266

Abstract

SGPT (Serum Glutamic Pyruvic Transaminase) is a liver enzyme that plays an important role in assessing hepatocellular damage. The stability of reagents, especially monoreagents consisting of a mixture of reagent 1 and reagent 2, affects the accuracy of laboratory test results. Delaying the use of monoreagent at room temperature may influence the enzymatic activity of SGPT. This study aims to determine the difference in SGPT levels between the monoreagent used immediately and the monoreagent delayed for 5 days at room temperature.This study used an experimental design with 16 serum samples from second-year students in the Diploma III Medical Laboratory Technology Program. SGPT levels were measured using the Clima MC-15 photometer. Two conditions were tested: monoreagent used immediately after mixing and monoreagent stored for 5 days at room temperature. Data were analyzed using the Shapiro-Wilk normality test and because the data were not normally distributed, the Wilcoxon test was used. The results showed that the average SGPT level using the immediate monoreagent was 10.69 U/L, while the delayed monoreagent showed an average of 9.88 U/L. Of the 16 samples, 9 decreased, 3 increased, and 4 remained unchanged. The Wilcoxon test produced a significance value of 0.030 (p < 0.05), indicating a statistically significant difference between the two conditions.In conclusion, there is a significant difference in SGPT levels immediate monoreagent use and delayed monoreagent use for 5 days at room temperature. It is recommended that monoreagent be used immediately after mixing to maintain accurate examination results.
Co-Authors Aprillia, Ima Ayudhawati, Reynindra Cakra Devi, Ledya Martha Dwi Niaga, Seftika  Endang Susanti Hartono Hartono Heryanti, Nabiilah Nuur Ainii Hidayah, Layla Nur Iwan Setiawan Kurniawati, Lutfi Nurhidayah, Intan Dwi Permada, Paundra Verend Pinto, Jennifer Da Costa Prarastri, Chalista Putri, Alya Ernita Putri, Salma Yuki Anisa Rachmawati, Tetiteti Salsabila, Lulu Sulfa Schatzi, Rachel Vastya Arma Shelomita, Maysa Putri Sitohang, Dahlan Tanaya, Angabaya Dera Sumba Wardani, Hesti Diah Ayu Kusuma Widyastuti, Maria Putri Dyaning Wulandhari, Septiani Tri