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All Journal Journal of Innovation and Applied Technology JFMR (Journal of Fisheries and Marine Research) Jurnal Akuakultura Universitas Teuku Umar JSMARTech : Journal of Smart Bioprospecting and Technology Juvenil: Jurnal Ilmiah Kelautan dan Perikanan Jurnal Pengabdian Masyarakat untuk Negeri (UN-PENMAS) Journal of Fish Health
Rahardjo, Seto Sugianto Prabowo
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Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Social Sciences Other

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Journal : JSMARTech : Journal of Smart Bioprospecting and Technology

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Antibacterial Activity of Ethyl Acetate Fraction of Butterfly Pea Flower (Clitoria ternatea) Against Aeromonas hydrophila in vitro Aprilyanti, Salsabila; Andayani, Sri; Rahardjo, Seto Sugianto Prabowo
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 6 No. 2 (2025): Vol. 6 No. 2 (2025): JSMARTech Volume 6, No 2, 2025
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2025.006.02.33

Abstract

Aeromonas hydrophila is a pathogenic bacterium responsible for significant losses in freshwater aquaculture. In response to the growing issue of antibiotic resistance, this study examined the antibacterial activity of the ethyl acetate fraction of butterfly pea flowers (Clitoria ternatea) against A. hydrophila in vitro. Extraction was performed using ethyl acetate, followed by Thin Layer Chromatography (TLC) and column chromatography, which yielded nine fractions. Among them, fraction 5 showed the highest antibacterial activity. Phytochemical screening revealed the presence of alkaloids, tannins, flavonoids, and triterpenoids. The Minimum Inhibitory Concentration (MIC) test revealed that 250 ppm was the lowest effective concentration, while the disk diffusion assay demonstrated that 350 ppm produced the largest inhibition zone (10.03 ± 1.27 mm), which was statistically comparable to that of chloramphenicol (F = 91.34; p < 0.01). Scanning Electron Microscopy (SEM) revealed structural damage to A. hydrophila cells, including membrane disruption and lysis. This study introduces a new approach utilizing the ethyl acetate fraction of C. ternatea, combined with fractionation techniques and SEM imaging, to evaluate antibacterial activity. It offers potential for the development of environmentally friendly natural antibacterial agents for the aquaculture industry Keywords: Clitoria ternatea, Aeromonas hydrophila, ethyl acetate fraction, antibacterial activity.
Co-Authors Abdul Aziz Aisyah, Jumrotul Alfarisi, Muhammad Asnin Aprilyanti, Salsabila Asus Maizar Suryanto H Denanta, I Made Kresna Diana Arfiati Ertikasari, Nuning Gigih Setia Wibawa Hariati, Anik Martinah Hariyadi, Hariyadi Idfi Setyaningrum, Idfi Kartini Kartini Kurnianingsih, Asmiyati Kurniawan, Hendra Agung Mahariawan, I Made Dedi Muhammad Dailami, Muhammad Muhammad Musa Nugroho, Nurul Oktaviani, Elsa Dian Patresna, Laelatul Nisa Permatasari, Felyta Dian Putra, Renanda Baghaz D.S. Putri, Ajeng Ayu Restuaning Rahmi Nurdiani Salsabila, Sivana Yesica Ayu Santoso, Ratna Amalia Satria, Ibnu Darma Savira, Riza setya widi ayuning permanasari, setya widi ayuning Sitti Aisyah Soko Nuswantoro Sri Andayani Suciati, Lusia Dyah Supriatin, Febriyani Eka TIWI NURJANNATI UTAMI Widyawati, Yuni Yenny Risjani Yudha, Hirmawan Tirta