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All Journal SAINS MEDIKA : JURNAL KEDOKTERAN DAN KESEHATAN MANGGALI
Wani, Elsayanti
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Education Environmental Science Health Professions Medicine & Pharmacology Social Sciences

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The Influence of Continuous Multi Stage Countercurrent Extraction Process (CMCE) Propolis Extract Administration on Superoxide Dismutase Activity and Hepatocytes Fibrosis Degree (An Experimental Study on Male Wistar Rats Induced with Carbon Tetrachloride (CCl4)) Wani, Elsayanti; Nasihun, Taufiqurrachman; Hussaana, Atina
Sains Medika: Jurnal Kedokteran dan Kesehatan Vol 11, No 1 (2020): June 2020
Publisher : Faculty of Medicine, Universitas Islam Sultan Agung (UNISSULA), Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1206.853 KB) | DOI: 10.30659/sainsmed.v11i1.5000

Abstract

Introduction: Continuous Multi Stage Countercurrent Extraction Process (CMCE) propolis extract containing Caffeic Acid Phenethyl Ester (CAPE) may inhibit the formation of lipid peroxidation. However, it is still unclear whether or not CMCE propolis is able to increase SOD activity and reduce acute hepatocytes damages induced by CCl4. Objective: To evaluate the influence of CMCE propolis extract administration in increasing the SOD activity and reducing the hepatocytes fibrosis of male Wistar rats induced with CCl4.Method: an experimental research with a post-test only control group design. Twenty eight male Wistar rats were divided into four groups. Group C-N was only induced with CCl4, while group CM-3, CM-7, and CM-14 were induced with CCl4 and received CMCE propolis extract respectively with 3.6 mg/200 g BW, 7.2 mg/200 g BW, and 14.4 mg/200 g BW. CMCE propolis was administered for 14 days and then CCl4 was administered on the last day. SOD activity was measured using the colorimetric method, while the hepatocytes fibrosis degree was assessed using Image J Software method with NASH score modification.Results: Post Hoc LSD analysis indicated that SOD activity in group CM-3 (51.67 ± 4.20), CM-7 (70.21 ± 6.26), CM-14 (80.85 ± 4.59) was lower than that in group C-N (23.70 ± 5.12) with P <0.05. Meanwhile, the Mann-Whitney U test showed that the hepatocytes fibrosis degree in group CM-3 (0.85 ± 0.69), CM-7 (1.14 ± 0.37), and CM-14 (0.71 ± 0.48) was significantly lower than that in group C-N (2.28 ± 1.25) with p<0.05.Conclusion: The CMCE propolis extract administration was able to increase the SOD activity and reduce the hepatocytes fibrosis degree of male Wistar rats induced with CCl4
PKM Penyuluhan Menjaga Imunitas saat Berpuasa pada Masa Pandemi dan Pemeriksaan Kesehatan Desa Sukorejo Kabupaten Pekalongan Lestari, Catur Retno; Elawati, Nunung Eni; Wani, Elsayanti; Bulan, Sahari
Manggali Vol 1 No 2 (2021): Manggali
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Ivet

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31331/manggali.v1i2.1672

Abstract

Ramadan fasting is a fast that must be done by Muslims during the month of Ramadan for a full month. Diet during Ramadan tends to change, due to differences in meal times compared to other months. The pandemic period coincides with the month of Ramadan which sometimes neglects health and diet during breaking and sahur. It is important to maintain immunity when fasting during a pandemic in order to improve health. This community service activity program is to increase knowledge and understanding of maintaining diet when fasting. The result of this activity was an increase in knowledge about health and continued with medical examinations, namely checking blood pressure, checking blood sugar levels, checking uric acid levels and checking cholesterol levels.
Co-Authors Atina Hussaana Bulan, Sahari Catur Retno Lestari Elawati, Nunung Eni Taufiqurrachman Nasihun