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All Journal E-Jurnal Medika Udayana The Indonesian Biomedical Journal Medica Hospitalia
Besin, Valentinus
Faculty Of Medicine, Universitas Surabaya, Jl. Raya Kalirungkut, Surabaya
Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Medicine & Pharmacology Nursing Public Health

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The Effect of Thymoquinone Administration on Local Immunoglobulin-G Levels of Rattus norvegicus Strain Wistar Sciatic Nerve Crush Injury Model Valentinus Besin; Abdul Hafid Bajamal; Mohammad Hasan Machfoed; Jusak Nugraha; Budi Utomo; Paulus Budiono Notopuro
The Indonesian Biomedical Journal Vol 14, No 1 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i1.1772

Abstract

BACKGROUND: Immunoglobulin-G (IgG) is a product of the initial response to secondary immune response, which accumulates in distal segment of the nerve after crush injury. Thymoquinone modulates the adaptive immune response. Effect of thymoquinone administration on local IgG levels of Rattus norvegicus Wistar rats sciatic nerve crush injury model has not been elucidated.METHODS: This was an experimental study, with 63 Rattus norvegicus Wistar rats that divided into 9 groups. Three groups were given placebo, 3 groups were given 100 mg/kg/day thymoquinone, and 3 groups were given 250 mg/kg/day thymoquinone. The rats were terminated based on the assigned group at 5x24, 6x24, and 7x24 hours and then the IgG levels were measured using sandwich enzyme-linked immunosorbent assay (ELISA).RESULTS: There was a significant difference in IgG levels after administration of 100 and 250 mg/kg/day thymoquinone at 5x24 hours and 7x24 hours post-injury compared to the rats that were given no treatment. A significant difference of IgG levels was also found after administration of 100 mg/kg/day thymoquinone group at 6x24 hours post-injury. Critical point of decreasing local IgG of all groups happened at 6x24 hours after injury, however, there was no significant difference in the median levels of thymoquinone at doses of 100 mg/kg and 250 mg/kg.CONCLUSION: Local IgG levels in distal segment of the sciatic nerve crush injury is lower in rats that were given 100 mg/kg thymoquinone treatment compared to the rats that receive no thymoquinone treatment since 5x24 hours after injury. Thymoquione administration should be given immediately after the crush injury until before 6x24 hours post-injury to decrease antibodies in degeneration process.KEYWORDS: thymoquinone, immunoglobulin-G, crush injury, sciatic nerve
Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model Valentinus Besin; Abdul Hafid Bajamal; I Ketut Sudiana; Mangestuti Agil; Jusak Nugraha; Mohammad Hasan Machfoed; Paulus Budiono Notopuro; Naesilla Naesilla
The Indonesian Biomedical Journal Vol 14, No 3 (2022)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v14i3.2011

Abstract

BACKGROUND: Interleukin (IL)-10 is involved in Wallerian degeneration after peripheral nerve crush injury. Oral thymoquinone was previously observed to decrease local immunoglobulin-G (IgG) in a crush-injured rat model. No study has evaluated the pathway of various thymoquinone dosages on local IgG and IL-10 in this injury.METHODS: This experimental study used 126 Rattus norvegicus Wistar rats that were divided into 18 groups: six groups received a placebo, the other six groups received thymoquinone at 100 mg/kg/day and the last six groups received thymoquinone at 250 mg/kg/day, respectively. Rats were sacrificed at 12, 18, 24, 5x24, 6x24, and 7x24 hours. Matrix metalloproteinase-9 (MMP-9), IL-10, and local IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). The nuclear factor KappaB (NF-κB) expressions on Schwann cells were examined by flow cytometry. Path analysis was performed using SmartPLS.RESULTS: The path analysis showed that 100mg/kg/day of thymoquinone significantly decreased NF-κB expression. However, NF-κB did not affect local MMP-9, and MMP-9 had no significant relationship with local IL-10 and IgG. Thymoquinone 250 mg/kg/day also significantly inhibited NF-kB expression, decreased local MMP-9, and, in turn, decreased local IL-10 and IgG.CONCLUSION:  Administration of oral thymoquinone 250 mg/kg/day decreases local IgG and IL-10 levels via suppressing NF-κB expression and MMP-9 levels.KEYWORDS: thymoquinone, crush injury, IgG, IL-10, MMP-9, NF-κB
Kantuk Berlebihan di Siang Hari dan Prestasi Akademik di Kalangan Mahasiswa Kedokteran Praklinik Fakultas Kedokteran Universitas Surabaya Besin, Valentinus; Sukmaya, Dita
E-Jurnal Medika Udayana Vol 12 No 9 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i09.P01

Abstract

Latar Belakang: Aktivitas akademik menyebabkan siklus tidur dan bangun yang tidak teratur serta kualitas tidur yang buruk pada mahasiswa kedokteran. Rasa kantuk berlebihan di siang hari pada siswa ini dapat menyebabkan rendahnya prestasi dan masalah emosional. Penelitian yang spesifik tentang gangguan tidur mahasiswa kedokteran praklinik dan performa akademik masih jarang. Penelitian ini bertujuan untuk melihat hubungan antara kantuk berlebihan di siang hari dan prestasi akademik mahasiswa kedokteran praklinik. Metode: Studi potong lintang ini melibatkan seluruh mahasiswa praklinik Program Studi Pendidikan Dokter Universitas Surabaya. Partisipan diminta mengisi formulir dalam Google Form yang berisi pertanyaan demografis dan Epworth-sleepiness scale (ESS). Indeks Prestasi Kumulatif (IPK) dikumpulkan dari Bagian Pendidikan Fakultas Kedokteran Universitas Surabaya. Skor ESS ? 10 dianggap mengalami rasa kantuk berlebihan di siang hari dan IPK ? 3.0 sebagai memuaskan. Uji chi-square digunakan untuk melihat perbedaan kejadian kantuk berlebihan di siang hari antar kelompok. Hasil: Secara total, terdapat 162 peserta yang didominasi oleh perempuan (74,1%). Rata-rata skor ESS adalah 10,62 ± 4,55. Berdasarkan skor ESS total, 57,4% partisipan mengalami kantuk berlebihan di siang hari. Tidak ada perbedaan IPK yang signifikan antara mahasiswa dengan dan tanpa kantuk berlebihan di siang hari (p = 0,620). Kesimpulan: Tingginya prevalensi kantuk di siang hari yang berlebihan pada populasi ini tidak berhubungan dengan IPK mahasiswa kedokteran praklinik. Studi selanjutnya harus mempertimbangkan sleep hygiene, faktor gaya hidup termasuk konsumsi teh atau kopi, dan fleksibilitas kognitif siswa.
Neuroimaging Findings in Patients with Covid-19 in Indonesia Sadikin, Cindy; Meryana, Meryana; Besin, Valentinus; Saelan, Yanna
Medica Hospitalia : Journal of Clinical Medicine Vol. 8 No. 1 (2021): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (380.59 KB) | DOI: 10.36408/mhjcm.v8i1.509

Abstract

Background: Covid-19 caused by the SARS-CoV-2 virus has spread worldwide, including Indonesia. Neurological manifestations has also been reported in Covid-19 positive patients. Yet documentation of their neuroimaging findings are lacking, especially in Indonesia. Objective: To understand neuroimaging findings in Covid-19 positive patients Methods: An observational study from medical record of Covid-19 positive patients in our hospital who developed abnormal neurologic manifestations and were followed up by neuroimaging examination from May to August 2020. Covid-19 positive diagnosis was confirmed from nasopharyngeal swab using the Real Time Polymerase Chain Reaction (RT-PCR). Neurological examination was performed by a neurologist, who then referred patients for neuroimaging examination using CT or MRI. Radiological expertise was performed by a radiologist. Results: A total of 288 patients who are Covid-19 positive from nasopharyngeal RT-PCR swab admitted to our hospital from May to August 2020. Ten patients (3.5%) had abnormal neurologic manifestations and further neuroimaging examination follow up. Range of age 33-72 years old and slight male predominance (60%). Frequent clinical symptoms were decreased consciousness (40%), altered mental status (30%) and tremors (20%). Neuroimaging findings were large vessel occlusion (30%), vasculitis (20%), post hipoxic leucoencephalopathy (10%), basal ganglia encephalopathy (10%), non specific small vessel ischemia changes and negative findings (30%). Most patients were discharged with clinical improvement (60%), while 40% mortality rate were seen in patient with large vessel occlusion (30%) and vasculitis (10%). Conclusion: Neuroimaging findings in Covid-19 positive patients were large vessel occlusion (LVO), vasculitis, post hipoxic leucoencephalopathy and basal ganglia encephalopathy Keywords: Covid-19, neurological, manifestation, neuroimaging
Co-Authors Abdul Hafid B Budi Utomo Jusak Nugraha Jusak Nugraha Mangestuti Agil Meryana, Meryana Mohammad Hasan Machfoed Naesilla Naesilla Notopuro, Paulus Budiono S.Pd. M Kes I Ketut Sudiana . Sadikin, Cindy Saelan, Yanna Sukmaya, Dita