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All Journal Media Penelitian dan Pengembangan Kesehatan Jurnal Kesehatan Masyarakat Folia Medica Indonesiana Jurnal Biosains Pascasarjana Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Biomedical Journal of Indonesia Jurnal Teknologi Laboratorium Journal Of Widya Medika Junior JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Narra J Jurnal Kedokteran Hewan
Jusak Nugraha
Department Of Clinical Pathology, Faculty Of Medicine, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Veterinary Other

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ASSOCIATION BETWEEN ATYPICAL DEPOLARIZATION IN CELLDYN 00 AND THE PRESENCE OF PLASMODIUM SPP IN BLOOD IN Dr. SOETOMO HOSPITAL SURABAYA Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 2, No 1 (2011)
Publisher : Institute of Topical Disease

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Abstract

Background: Malaria is a parasitic disease worldwide with a high morbidity and mortality. A rapid and accurate method is needed to detect the presence of malaria parasites in blood. A flagging system atypical depolarization (atypdep) in CBC results from Cell-Dyn 3200 has been related with malaria infection. Materials and Methods: An observational cross sectional approach with 48 samples obtained from inpatients of the Dr.Soetomo Hospital, Surabaya. Samples were screened by Cell-Dyn 3200 analyzer for atypdep flagging in CBC. Positive samples were later confirmed by microscope to detect malaria parasites. results: From 48 samples with atypdep flagging, 7 samples were malaria positive on peripheral blood smear (13.1%). Most frequent atypdep flagging was seen in malignancy (18.7%), and approximately 54.6% of the samples were not accompanied by fever symptoms. Lekositosis and anemia each were found in 20 samples (41.6%) and thrombocytopenia in 33.3%. conclusion: The presence of atypdep flagging in Cell-Dyn 3200 does not necessarily indicate the existence of malaria or it could be said that atypdep flagging is not always associated with presence of malaria infection. The usage of an atypdep flagging in non-endemic areas such as Surabaya is just an alert sign to evaluate malaria infection rather than a screening method to detect malaria.
PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

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Abstract

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of the most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bag from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet count could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300 rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.
UPAYA OPTIMASI PEMBUATAN PLASMA KAYA TROMBOSIT SEBAGAI PENGOBATAN SEL PUNCA Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Hans K; Nugraha, Jusak
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.480

Abstract

Stem cells have a remarkable potential to act as a self renewal in the related system. The proliferation of stem cells can be stimulated by Platelet Rich Plasma (PRP) growth factor. PRP has a potential application for aesthetics, traumatology and maxillofacial surgery. The optimation method could be carried out by a double centrifugation, which is reliable and simple in producing an optimal PRP. The samples were obtained from healthy volunteers; 10 mL of blood was needed to produce 1 mL PRP. CBC test was then performed, in order to know the platelet count before and after double centrifugation. This method used four variations of speed and time. Four variations of speed and time were used to find the optimation result, which yield the highest platelet count and the highest PDGF level released after platelet activation. The measurement of Platelet Derived Growth Factor (PDGF-BB) level was done using ELISA method. Twenty first samples, resulted in a variation of platelet counts, the mean was 0.89 times. About 60% of platelet counts showed a decrease with the lowest value 0.01 times and 40% of the platelet counts showed an increase with the highest value 3.94 times. The study was repeated using “I” tubes, the highest increase of platelet count and PDGF-BB level was obtained by centrifugation at 900 g, 5 minutes duration, then followed by 1500 g, for 15 minutes. In this study protocol, the production of optimal PRP was not yet found, however it provided some important information. In this case, the influence of separation process and skill was more important than the centrifugation speed. The researchers suggested that the measurement of PDGF-BB level should be done immediately after PRP harvest.
History Of Tuberculosis Contact And Tuberculin Test Result Saputra, Angky; Nugraha, Jusak; Darmadi, Epriyanto T
JOURNAL OF WIDYA MEDIKA JUNIOR Vol 1, No 3 (2019)
Publisher : FAKULTAS KEDOKTERAN UNIVERSITAS KATOLIK WIDYA MANDALA SURABAYA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (897.833 KB) | DOI: 10.33508/jwmj.v1i3.2100

Abstract

Introduction: Tuberculosis (TB) is a chronic disease caused by Mycobacterium tuberculosis. History of tuberculosis contact has consequences for TB infection. Density of occupancy may also accelerate TB transmission. Tuberculin test is a screening method that can detect TB infection. However, BCG immunization can reduce the specificity of tuberculin test results because it causes false positive results. Aim: The aim of this study is to see the relationship between history of TB contact and tuberculin test results. Other variables related to tuberculin test results were also examined i.e. occupancy density and incidence of BCG immunization. Methods: This research was conducted with cross-sectional analytic design using contingency coefficient test with a value of ? = 0.1. TB contacts subjects were taken from Surabaya Pulmonary Hospital and non-TB contacts subjects were volunteers from Medical Faculty of Widya Mandala Catholic University volunteers and Medical Faculty of Wijaya Kusuma. Each group consisted of 25 people taken using consecutive sampling technique. There is a significant correlation between history of tuberculosis contact and tuberculin test (p = 0,000); There is no significant correlation between BCG immunization history and tuberculin test results (p = 0.221) Result: There is a significant correlation between occupancy density and tuberculin test results (p = 0.066). Conclusion: It can be concluded that history of tuberculosis contact and occupancy density are associated with positive tuberculin test results.
Correlation of C3c complement, NT-pro BNP and Left Ventricle Ejection Fraction in Heart Failure Julious, Julious; Nugraha, Jusak; Aminuddin, Mohammad
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 26, No 1 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v26i1.1341

Abstract

Introduction. Heart failure is a health problem in Indonesia. The 2013 Basic Health Research Data showed that the estimated heart failure in Indonesia was 530,068 people. Echocardiography examination which has been a routine examination of heart failure patients is not necessarily available in all hospitals, so that a more applicable and inexpensive alternative examination is needed. Previous studies have shown an increase in C3c levels associated with improved survival and better cardiac remodeling. On this basis, this research needs to be performed in order to determine the correlation between C3c complement levels, NT-proBNP and LVEF in heart failure patients. Method. A cross-sectional study was conducted at the Dr Soetomo Hospital between August 2018 to September 2018 with 30 samples. Samples were taken consecutively from patients with heart failure who were treated at the integrated heart service center. Examination of C3c complement, NT-proBNP and echocardiography (LVEF data) was carried out in all patients. Result. The result of this study showed no significant correlation between C3c complement and NT-proBNP (r = -0.253, p = 0.177). The correlation between C3c complement and LVEF was also not significant (r = -0.074, p = 0.696). A significant moderate correlation between NT-proBNP and LVEF was found (r = -0.444, p = 0.014). Conclusion. The C3c complement could not be used as an alternative examination for NT-proBNP and LVEF. The limitations of the study were heterogeneous sample characteristics. A further study with more stringent criteria is needed to minimize the bias of examination results.
Chymase Level in Dengue Virus Infection With or Without Positive Non-Structural 1(NS1) Kasiyati, Menik; Nugraha, Jusak; Kahar, Hartono
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1227.342 KB) | DOI: 10.29238/teknolabjournal.v8i2.167

Abstract

Thrombocytopenia, leukopenia, and monocytosis are laboratory parameters in the diagnosis of dengue virus infection. In addition to monocyte cells, mast cells also play a role in the innate immune response, where degranulation of mast cells will occur, which will secretion the active vaso mediator, Chymase. Chymase has a role in increasing vascular permeability resulting in plasma leakage in patients with dengue virus infection to determine the number of platelets, leukocytes, monocytes and chymase levels in patients with dengue infection in the acute phase. The platelet count mean in NS1 (+) was 132,140 cells / mm3 and the platelet count in the NS1 group was (-) 176,000 cells / mm3. The mean leukocytes NS1 (+) showed results of 4,350 cells / mm3 and NS1 (-) 5,250 cells / mm3. The mean monocyte NS1 (+) monocyte count was 8.26%, and NS1 (-) group was 8.76%. There were no significant differences in platelet counts, leukocytes and monocytes between NS1 (+) and NS1 (-) (P value> 0.05). The mean Chymase NS1 (+) 23.48, NS1 (-) 23.05 ng / mL and the control group 1.47ng / mL.
The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant Buana, Dwi Candra; Sudiana, I Ketut; Nugraha, Jusak
Jurnal Teknologi Laboratorium Vol 8 No 2 (2019): 2019 (2)
Publisher : POLTEKKES KEMENKES YOGYAKARTA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1954.413 KB) | DOI: 10.29238/teknolabjournal.v8i2.177

Abstract

The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.
IFN-GAMMA SECRETION AND IL-10 AFTER STIMULATION OF ESAT-6-CFP-10 (EC610) FUSION ANTIGEN IN PATIENTS WITH ACTIVE TUBERCULOSIS AND LATENT TUBERCULOSIS Prihantika S., Sabrina; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, M Irsan; Hafy, Zen; Tanoerahardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 5, No 3 (2019)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v5i3.8897

Abstract

Tuberculosis (TB) is an infectious disease caused by the bacterium Mycobacterium tuberculosis which is still a health problem in the world including in Indonesia. TB infection arises due to interference with the regulation of the body's defense system. Cellular immune system plays a role in fighting TB infection, namely the role of T lymphocytes that differentiate into Th1 cells secrete pro-inflammatory cytokines IFN-? and Th2 cells that secrete IL-10 anti-inflammatory cytokines. The EC610 fusion antigen is specific and has a strong antigenicity to T cell stimulation. The aim of the study was to determine differences in the mean levels of IFN-? and IL-10 after EC610 antigen stimulation in patients with active TB and latent TB. The design of this study was a quasi-in vitro experiment with PBMC culture stimulated by EC610 antigens in the active TB and latent TB groups. The research and examination were carried out at the South Sumatra Province SSR and the Health Research and Development Laboratory Center, Central Jakarta. There were 21 subjects with active TB and 28 subjects with latent TB who met the inclusion criteria. Measurement of IFN-levels? and IL-10 was performed using ELISA-Reader. The results showed the levels of IFN-? and IL-10 after EC610 antigen stimulation were higher in active TB than latent TB. High levels of IFN-? active TB patients showed protective immune responses against M.tb germs while high levels of IL-10 showed their role as anti-inflammatory. There is no significant difference in the average level of IFN-? in patients with active TB and latent TB (p = 0.769) and there was a significant difference in the level of IL-10 levels after EC610 antigen stimulation was higher in active TB than latent TB (p = 0,000).
IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

Abstract

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.
IFN-GAMMA AND IL-2 SECRETION AFTER ESAT-6-CFP-10 (EC-610) FUSION ANTIGEN STIMULATION FROM PATIENTS WITH ACTIVE LUNG TUBERCULOSIS AND LATENT LUNG TUBERCULOSIS Darwin, Bastian; Kurniati, Nova; Rahadiyanto, Kemas Ya'kub; Saleh, Mgs Irsan; Tanoehardjo, Francisca Srioetami; Nugraha, Jusak; Salim, Eddy Mart
Biomedical Journal of Indonesia Vol 6, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bji.v6i2.10741

Abstract

The Secretion of IFN-Gamma and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-gamma and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-gamma assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-gamma levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.
Co-Authors Abdul Hafid B Agung Dwi Wahyu W Agustiningrum, Indah aminuddin, mohammad Anak Agung Istri Sri Wiadnyani Anita Budiarti Anna Roosdiana Aryati Aryati Bastian Bastian Beatrix, Stephanie Besin, Valentinus Betty Agustina Tambunan Buana, Dwi Candra Budi Prasetyo Budi Utomo Budiutari, Ni Nyoman Budy, Theresia Indah Callixte, Cyuzuzo Caroline Caroline Cynthia Cynthia Cynthia Darmadi, Epriyanto T Dwi Aris Agung Nugrahaningsih Dwi Liliek Kusindarta Dwiyanti Puspitasari, Dwiyanti Eddy Mart Salim, Eddy Mart Edhi Rianto Elok Budi Retnani Foe, Kuncoro Handayani, Luh Putu Trys Monika Hans Kristian Nugraha, Hans Kristian Hartono Kahar, Hartono Hendy Wijaya Heru Setiawan Hevi Wihadmadyatami Huda Shalahudin Darusman I Made Andika Bara Kusuma Ilham Ilham Januarti, Catur Ifda julious, julious Kasiyati, Menik kurniati, nova Lisa Soegianto Lumempouw, Silvia Luminto, Dian Meiti Muljanti, Meiti Mohammad Hasan Machfoed Muhammad Hamdan Muhammad Irsan Saleh Muhammad Nazarudin Nico Jafet Ningrum, Emilna Mega Notopuro, Paulus Budiono Noviana, Rachmitasari Nugraha, Hans K Oetami, Fransisca Sri Oryza Chrisantia Pratama, Dyah Ayu Oktaviani A Prihantika S., Sabrina Pudji Lestari Purwanta, Marijam Putri, Indah Aprianti Rahadiyanto, Kemas Ya'Kub Rahajuningsih Dharma Rendy, Achmad Nur Rifa’I, Muh Husni Rusli, Musofa Ryzky Widi Atmaja Saleh, M Irsan Saputra, Angky Saputri, Agatha Efrad Senny Yesery Esar Setiawan, Lidwina Tri Kristanti Shoukat, Hamad Shoukat, Shahzad SILMI MARIYA Silvia F. Lumempouw Sinansari, Restry Siti Rahmawati Soedarsono Soedarsono Srikanth Karnati Süleyman Ergün Tanoehardjo, Francisca Srioetami Tanoehardjo, Francisca Srioetami Tanoerahardjo, Francisca Srioetami Tedja, I G.A. Wiradari Utariani, Arie UUS SAEPULOH Wahyu Dewi Tamayanti, Wahyu Dewi Winthoko, Eka Nora Vitaloka Aprilia Putri Wulandari, Dian Novita Wuryanto Hadinugroho Yetti Hernaningsih Yoes Prijatna Dachlan Yudy Tjahjono Yufita Ratnasari Wilianto Zen Hafy