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All Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML)
Nunki, Nastasya
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Biochemistry, Genetics & Molecular Biology Health Professions Medicine & Pharmacology Neuroscience

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The Predominance of the Synthesis Phase in Cell Cycles in Childhood Acute Lymphoblastic Leukemia Hernaningsih, Yetti; Aryati, Aryati; Ugrasena, I Dewa Gede; Nunki, Nastasya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 3 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i3.2360

Abstract

Uncontrolled proliferation and resistance to apoptosis should be controlled to kill the cancer cells, as the goal of treatment in acute leukemia. It is therefore important to know, which characteristic dominates the proliferative phase because, to be more effective, new drugs that will be developed should be targeted at that phase. This study aimed to find the dominant phase in the proliferative phase of the cell cycle in childhood Acute Lymphoblastic Leukemia (ALL). This study was conducted on 63 patients, comprising 33 patients with specimens of Bone Marrow Mononuclear Cells (BMMC), 30 patients with specimens of Peripheral Blood Mononuclear Cells (PBMC), and 10 children as controls with specimens of PBMC. Cell cycle examinations were performed with PI/RNase, run on BD FACS Calibur, and analyzed with modfit LT 4.1 software to determine the phases of G0/G1, synthesis (S), and mitosis (G2/M). Regarded the S and G2/M phases as the proliferation phase. Statistical analyses used one-way ANOVA and Kruskal-Wallis to compare S and G2/M phases within groups,and an Independent T-test and Mann-Whitney to compare the same phases between groups. Among the proliferation phases, the synthesis phase had significantly higher domination than the mitotic phase in both the BMMC (10.18±7.81% vs. 5.41±3.77%; p = 0.010) and PBMC (4.33±2.15% vs 2.30±1.56%; p=0.000) groups. A similar result was found in the control group (1.01±0.51% vs 0.36±0.38%; p=0.011). The synthesis phase in BMMC had significantly higher domination than PBMC groups (p=0.024) and control (p 0.002). The synthesis phase predominates among the proliferation phases of the cell cycle in childhood ALL.
The Role of Interleukin-7 in T Cell Acute Lymphoblastic Leukemia: A Systematic Literature Review Primartati, Danny Meganingdyah; Hernaningsih, Yetti; Nunki, Nastasya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 31 No. 3 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v31i3.2500

Abstract

This study aims to provide a comprehensive review of the genetic characteristics of acute lymphoblastic leukemia that has relapsed, discuss the relationship between interleukins and the disease, and explore targeted therapy. The Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) 2020 guidelines were followed in the development of the current study on acute lymphoblastic leukemia and the role of interleukins in hemato-oncology. The results reveal that relapse rates remain high and the prognosis for individuals who experience a relapse is inferior. Currently, innovative targeted medicines are employed to supplement traditional cancer treatment. The novel strategies are put into practice to lessen the load of toxicity and enhance results. From these findings, it can be concluded that combating refractory T-ALL requires the discovery of innovative medicines that directly target pathways driving Leukemia-Initiating Cell (LIC) activity.
Comparison of Proliferation and Apoptosis in CD34+ Lymphoblasts in Pediatric Acute Lymphoblastic Leukemia: invivo and exvivo Conditions Hernaningsih, Yetti; Cahyadi, Andi; Rusanti, Rahmi; Armayani, Erawati; Juwita, Syntia Tanu; Nur‘ Aini, Farida; Tanzilia, May Fanny; Nunki, Nastasya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 32 No. 1 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v32i1.2389

Abstract

The proliferation and apoptosis assays have been utilized in numerous studies to develop new substances such as antineoplastic agents. Commonly, it was performed in ex-vivo conditions using the culture method. However, the cytotoxic or cytostatic effects observed ex vivo often differ from those in vivo. This study investigated differences in proliferation and apoptosis of lymphoblast between in vivo and ex vivo conditions of childhood ALL. This study was conducted on new childhood acute lymphoblastic leukemia (ALL) patients. Nineteen (19) subjects were recruited, comprising of 10 with favorable and 9 with unfavorable outcomes. The negative control came from 8 healthy children volunteers. All patients under went leukemia immunophenotyping, including CD34, to identify the phenotype. Bone marrow mononuclear cells (BMMCs) of patient groups were analyzed using apoptosis and proliferation assays, as well as the negative control group, and then compared to the in vivo condition. 12 out of 19 BMMC were cultured for 48 hours, and proliferation and apoptosis assays were performed in ex vivo conditions. The results showed that the proliferation, apoptosis, and apoptosis/proliferation (A/P) ratio of the patients in the ex vivo group were significantly higher than the in vivo group with p =0.000, p =0.050, and p =0.000, respectively. The proliferation was higher for patient groups than the control group, with p =0.001 and p =0.004, respectively. The apoptosis rates of the patient group were higher than the control group, with p =0.000 and p =0.002, respectively. The proliferation and apoptosis of lymphoblasts ex vivo are higher than in vivo.
Co-Authors Andi Cahyadi Armayani, Erawati Aryati Aryati Danny Meganingdyah Primartati I Dewa Gede Ugrasena Juwita, Syntia Tanu Nur‘ Aini, Farida Rahmi Rusanti, Rahmi Tanzilia, May Fanny Yetti Hernaningsih