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CD4+ T-cell, CD8+ T-cell, CD4+ /CD8+ ratio, and apoptosis as a response to induction phase chemotherapy in pediatric acute lymphoblastic leukemia May Fanny Tanzilia; Andi Cahyadi; Yetti Hermaningsih; Endang Retnowati; I Dewa Gede Ugrasena
Paediatrica Indonesiana Vol 57 No 3 (2017): May 2017
Publisher : Indonesian Pediatric Society

Background Acute lymphoblastic leukemia (ALL) is a neoplastic disease resulting from somatic mutation in the lymphoid progenitor cells, often occuring in children aged 2-5 years, predominantly in males. Results from the induction phase of chemtherapy are used to measure success, but the failure remission rate is still high. Increased apoptosis of cancer cells, as induced by CD4+ and CD8+T-cells, is an indicator of prognosis and response to chemotherapy.Objective To assess for correlations between CD4+, CD8+, or CD4+/CD8+ ratio to the chemotherapy induction phase response (i.e., apoptosis) in pediatric ALL patients.Methods This observational analytical cohort study was done in 25 pediatric ALL patients. Whole blood (3 mL) with EDTA anticoagulant were used to measure absolute counts of CD4+, CD8+, and CD4+/CD8+ ratio. Peripheral blood mononuclear cells (PBMC) were examined for apoptosis. The principle of CD4+, CD8+ examination was bond between antigens on the surface of the leukocyte in the blood with fluorochrome labeled antibodies in the reagents, while the principle of apoptosis examination was FITC Annexin V will bonds with phosphatidylserine that moves out of the cell when the cell undergoes apoptosis, then intercalation with propidium iodide (PI). All examination were detected by flow cytometry BD FACSCalibur.Results Subjects were 25 newly-diagnosed, pediatric ALL patients (64% males and 36% females). Most subjects were 3 years of age (20%). Numbers of CD4+ and CD8+ cells, as well as CD4+/CD8+ were significantly decreased after chemotherapy. However, apoptosis was not significantly different before and after chemotherapy (P=0.689), There were significant negative correlations between apoptosis and CD4+ (P=0.002; rs=-0.584), and CD8+ (rs=-0.556; P=0.004), before chemotherapy. In addition, CD4+-delta and apoptosis-delta also had a significant positive correlation (rs=0.478; P=0.016). However, no correlation was found between the CD4+/CD8+ ratio and apoptosis, before or after chemotherapy.Conclusion There are significantly lower mean CD4+, CD8+, and CD4+/CD8+ ratio after chemotherapy than before. Also, there are significant correlations between CD4+-delta and apoptosis-delta, as well as between apoptosis and CD4+, CD8+, and CD4+/CD8+, before chemotherapy. CD4+, CD8+, and CD4+/CD8+ can be used to predict apoptosis before chemotherapy. In addition, CD4+-delta can be used to predict apoptosis-delta as a response to induction phase chemotherapy in pediatric ALL.

PENGUATAN DAN PEMANTAPAN KOMPETENSI PARASITOLOGI PADA GURU BIOLOGI SEKOLAH MENENGAH ATAS Hebert Adrianto Rubay; Yuwono Marta Dinata; May Fanny Tanzilia; Billy Daniel Messakh; Ronald Torang M. Panggabean
Community Development Journal : Jurnal Pengabdian Masyarakat Vol. 3 No. 1 (2022): volume 3 Nomor 1 Tahun 2022
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/cdj.v3i1.3191

Guru merupakan aset negara yang sangat penting dalam mencerdaskan anak bangsa. MGMP Biologi Sidoarjo adalah mitra rutin pengabdian masyarakat selama dua tahun. Penguatan materi yang pernah diberikan diulang kembali dengan cara dikemas dalam bentuk olimpiade. Tujuan kegiatan adalah memberikan penguatan dan pemantapan pengetahuan para guru biologi pada topik parasitologi. Metode kegiatan adalah difusi ipteks, pendampingan, dan penyelenggaraan kegiatan kompetisi olimpiade. Olimpiade dibagi menjadi babak penyisihan dan babak final. Properti yang digunakan adalah buku, power point, autoproctor, zoom, youtube. Hasil kegiatan ini adalah telah didapatkan lima juara, kompetisi berlangsung meriah dan anggota memberikan dukungan. Para guru menilai kegiatan ini sangat memberikan manfaat karena mengingat kembali materi yang telah dipelajari, mengukur pemahaman materi parasitologi, memotivasi guru untuk terus semangat belajar dan mengukir prestasi, dan anggota MGMP lebih aktif terlibat.

Penguatan Pengetahuan Tentang Fauna Nyamuk Pada MGMP Guru Biologi SMA Hebert Adrianto Adrianto; Sri Subekti Subekti; Heny Arwati Arwati; Indah S Tantular; Yuwono Marta Dinata; May Fanny Tanzilia; Wira Widjaya Lindarto; Amadeus Michel Goein; Nathania Disa Ariesta Andriani
Jurnal Kreativitas Pengabdian Kepada Masyarakat (PKM) Vol 5, No 2 (2022): Volume 5 No 2 Februari 2022
Publisher : Universitas Malahayati Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jkpm.v5i2.4596

ABSTRAK Penyakit Demam Berdarah Dengue (DBD) menimbulkan sakit sehingga menghentikan produktivitas manusia, kematian, bahkan wabah (Kejadian Luar Biasa/ KLB). DBD ditularkan melalui perantaraan nyamuk. Tujuan kegiatan pengabdian masyarakat ini adalah memberikan penguatan materi kepada para guru tentang bahaya dan kewaspadaan terhadap nyamuk. Kegiatan ini dilaksanakan secara daring menggunakan zoom. Peserta kegiatan ini adalah semua guru mata pelajaran Biologi yang tergabung di dalam MGMP Biologi SMA Kabupaten Sidoarjo. Instrumen yang digunakan adalah laptop, materi presentasi format ppt, zoom, dan google form. Hasil kegiatan adalah kegiatan berjalan dengan lancar. Ada peningkatan pengetahuan para peserta dari dimana rata-rata nilai pre test = 53 dan post test = 81. Kata Kunci: Nyamuk, guru, pengetahuan ABSTRACT Dengue hemorrhagic fever (DHF) causes pain that stops human productivity, death, even plague. DHF is transmitted through mosquito intercession. These community service activities aim to provide material reinforcement to teachers about the dangers and vigilance against mosquitoes. This activity is carried out online using zoom. Participants of this activity are all teachers of Biology subjects who are members of MGMP Biology SMA Sidoarjo. The instruments used are laptop, ppt format presentation material, zoom, and google form. The result of the activity is that the training runs smoothly. There was an increase in the participants' knowledge from which the average pre-test value= 53 and post-test = 81. Keywords: Mosquitoes, teachers, knowledge

TINJAUAN PUSTAKA: PATOGENESIS DAN DIAGNOSIS SISTEMIK LUPUS ERITEMATOSUS May Fanny Tanzilia; Betty Agustina Tambunan; Desak Nyoman Surya Suaemitria Dewi
Syifa'Medika Vol 11, No 2 (2021): Syifa' MEDIKA: Jurnal Kedokteran dan Kesehatan
Publisher : Faculty of Medicine

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/sm.v11i2.2788

Sistemik Lupus Eritematosus (SLE) adalah penyakit inflamasi autoimun kronis dengan manifestasi klinis yang luas. Perjalanan penyakit dan prognosis dari SLE pun juga beragam. Faktor lingkungan, imunologi, hormonal, dan genetik diketahui memegang peranan dalam perkembangan SLE. Penyakit SLE lebih banyak menyerang wanita terutama usia produktif. Patogenesis SLE mengikutsertakan berbagai sel dan molekul yang berperan pada proses apoptosis, respons imun innate dan adaptif. Diagnosis SLE ditegakkan berdasarkan manifestasi klinis, yang harus memenuhi 4 dari 11 kriteria American Rheumatology Association (ARA) (1997), dan pemeriksaan laboratorium. Terapi SLE bersifat individual berdasarkan manifestasi klinis yang dialami pasien, aktivitas penyakit dan derajat keparahan penyakit serta komorbiditas. Prognosis SLE bervariasi mulai dari ringan hingga berkembang cepat menjadi berat disertai kegagalan multiorgan bahkan kematian. Studi pustaka ini diharapkan dapat memberikan informasi lebih mendalam mengenai patogenesis dan cara menegakkan diagnosis SLE sehingga dapat menjadi dasar dalam pengembangan penelitian mengenai SLE di masa yang akan datang.

Penguatan pengetahuan guru Biologi dalam penanganan kutu kepala (Pediculus humanus capitis) Adrianto, Hebert; Tanzilia, May Fanny; Lindarto, Wira Widjaya; Dinata, Yuwono Marta; Goein, Amadeus Michel; Andriani, Nathania Disa Ariesta
Jurnal Pengabdian Masyarakat: Humanity and Medicine Vol 2 No 2 (2021): Jurnal Pengabdian Masyarakat: Humanity and Medicine
Publisher : Fakultas Kedokteran Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/Hummed.V2I2.59

Head lice infestation (Pediculus humanus capitis) is an obligate ectoparasite infestation in human hair. The Sidoarjo Biology MGMP has been working with the service team for three years. The interview results were obtained with the management of the Sidoarjo Biology MGMP the need to strengthen knowledge about head lice. This activity aims to improve the understanding of head lice (Pediculus humanus capitis) for biology teachers in the Sidoarjo district. Training activities are carried out online using Zoom. The form of activities in the form of material delivery, questions and answers, and tests. The result of this service is that there is an increase in participants' knowledge from the average value of 62.75 to 84.13. Most of the teachers have come across cases and have given initial action to pediculosis sufferers. In general, the training event went smoothly and received a positive response.

Effect Of Coffee Drinking Habit to Blood Pressure and Hemoglobin Levels on Women of Childbearing Age Wartiningsih, Minarni; Brotosudarmo, Tatas Hardo Panintingjati; Gonaldy, Vincent Aurelius; Juliasih, Ni Njoman; Silitonga, Hanna; Tanzilia, May Fanny
Journal of Epidemiology and Public Health Vol. 9 No. 3 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.03.01

Background: Tenggerese people, especially women, have a habit of drinking coffee, and it has become a daily habit. Coffee is often associated with risk factors for diseases in the community such as anemia and hypertension. This study aims to determine the effect of coffee drinking quantity (1-2 cups, 3-4 cups, and ≥ 5 cups of coffee per day) on hemoglobin levels and blood pressure. Subjects and Method: A quantitative study with a cross-sectional study design was done. This study population was women of reproductive age (15 to 49 years) in Ngadipuro Hamlet, Tengger, East Java, Indonesia. The independent variable of this study is the of daily coffee drinking per cup and the dependent variable of this study is the hemoglobin levels in women of childbearing age. The study instrument used was a questionnaire to screen for those who were a daily coffee drinker and a blood hemoglobin rapid test kit was used to measure the level of hemoglobin.The number of samples in this study was 193 respondents which was chosen using the random sampling technique. Analysis of the quantity of coffee drinking on hemoglobin levels by the Annova test while on blood pressure by the Kruskal Wallis test through SPSS software. Results: The result showed that the quantity of coffee per day on hemoglobin levels had a significance p<0.001. While the quantity of coffee per day on blood pressure had a significance p=0.361. Conclusion: It can be concluded that drinking 1-2 cups of coffee, 3-4 cups, and ≥ 5 cups of coffee daily simultaneously affects hemoglobin levels. In this case, the more quantity of coffee per day, the lower the hemoglobin level. However, the quantity of coffee had no significant effect on blood pressure.

Comparison of Proliferation and Apoptosis in CD34+ Lymphoblasts in Pediatric Acute Lymphoblastic Leukemia: invivo and exvivo Conditions Hernaningsih, Yetti; Cahyadi, Andi; Rusanti, Rahmi; Armayani, Erawati; Juwita, Syntia Tanu; Nur‘ Aini, Farida; Tanzilia, May Fanny; Nunki, Nastasya
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 32 No. 1 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v32i1.2389

The proliferation and apoptosis assays have been utilized in numerous studies to develop new substances such as antineoplastic agents. Commonly, it was performed in ex-vivo conditions using the culture method. However, the cytotoxic or cytostatic effects observed ex vivo often differ from those in vivo. This study investigated differences in proliferation and apoptosis of lymphoblast between in vivo and ex vivo conditions of childhood ALL. This study was conducted on new childhood acute lymphoblastic leukemia (ALL) patients. Nineteen (19) subjects were recruited, comprising of 10 with favorable and 9 with unfavorable outcomes. The negative control came from 8 healthy children volunteers. All patients under went leukemia immunophenotyping, including CD34, to identify the phenotype. Bone marrow mononuclear cells (BMMCs) of patient groups were analyzed using apoptosis and proliferation assays, as well as the negative control group, and then compared to the in vivo condition. 12 out of 19 BMMC were cultured for 48 hours, and proliferation and apoptosis assays were performed in ex vivo conditions. The results showed that the proliferation, apoptosis, and apoptosis/proliferation (A/P) ratio of the patients in the ex vivo group were significantly higher than the in vivo group with p =0.000, p =0.050, and p =0.000, respectively. The proliferation was higher for patient groups than the control group, with p =0.001 and p =0.004, respectively. The apoptosis rates of the patient group were higher than the control group, with p =0.000 and p =0.002, respectively. The proliferation and apoptosis of lymphoblasts ex vivo are higher than in vivo.

Effect Of Coffee Drinking Habit to Blood Pressure and Hemoglobin Levels on Women of Childbearing Age Wartiningsih, Minarni; Brotosudarmo, Tatas Hardo Panintingjati; Gonaldy, Vincent Aurelius; Juliasih, Ni Njoman; Silitonga, Hanna; Tanzilia, May Fanny
Journal of Epidemiology and Public Health Vol. 9 No. 3 (2024)
Publisher : Masters Program in Public Health, Universitas Sebelas Maret, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26911/jepublichealth.2024.09.03.01

Background: Tenggerese people, especially women, have a habit of drinking coffee, and it has become a daily habit. Coffee is often associated with risk factors for diseases in the community such as anemia and hypertension. This study aims to determine the effect of coffee drinking quantity (1-2 cups, 3-4 cups, and ≥ 5 cups of coffee per day) on hemoglobin levels and blood pressure. Subjects and Method: A quantitative study with a cross-sectional study design was done. This study population was women of reproductive age (15 to 49 years) in Ngadipuro Hamlet, Tengger, East Java, Indonesia. The independent variable of this study is the of daily coffee drinking per cup and the dependent variable of this study is the hemoglobin levels in women of childbearing age. The study instrument used was a questionnaire to screen for those who were a daily coffee drinker and a blood hemoglobin rapid test kit was used to measure the level of hemoglobin.The number of samples in this study was 193 respondents which was chosen using the random sampling technique. Analysis of the quantity of coffee drinking on hemoglobin levels by the Annova test while on blood pressure by the Kruskal Wallis test through SPSS software. Results: The result showed that the quantity of coffee per day on hemoglobin levels had a significance p<0.001. While the quantity of coffee per day on blood pressure had a significance p=0.361. Conclusion: It can be concluded that drinking 1-2 cups of coffee, 3-4 cups, and ≥ 5 cups of coffee daily simultaneously affects hemoglobin levels. In this case, the more quantity of coffee per day, the lower the hemoglobin level. However, the quantity of coffee had no significant effect on blood pressure.

Hereditary Persistence of Fetal Hemoglobin (HPFH) in Community Tanzilia, May Fanny; Wartiningsih, Minarni
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i2.15191

Hereditary Persistence of Fetal Hemoglobin adalah kasus yang jarang terjadi di komunitas. Kondisi heterogen ini ditandai dengan produksi hemoglobin fetus (Hb F) yang menetap hingga dewasa dimana seharusnya pada usia dewasa produksi hemoglobin dewasa (Hb A) yang mendominasi. HPFH diklasifikasikan dalam dua bentuk yaitu delesi dan non delesi. HPFH dapat dianggap sebagai bentuk talasemia Î² atau Î´Î² ringan karena kegagalan sintesis rantai Î² dikompensasi oleh rantai Î³ setelah periode neonatal. Hb F dapat meningkat pada dewasa akibat kelainan herediter sintesis hemoglobin maupun kondisi dapatan (acquired) dari diagnosis banding kelainan hemoglobin lainnya. Diagnosis HPFH berdasarkan pada keadaan klinis, pemeriksaan darah rutin, dan pemeriksaan laboratorium khusus yaitu dengan mengukur kadar Hb F dan distribusi interseluler Hb F. Metode pemeriksaan Hb F dapat dilakukan dengan metode elektroforesis hemoglobin. Pemeriksaan Hb F bertujuan untuk menegakkan diagnosis adanya kelainan herediter rantai globin dan kondisi dapatan (acquired) lainnya, dimana pada keadaan tersebut, kadar Hb F akan meningkat. Untuk itu diperlukan pengukuran kadar Hb F secara tepat.

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