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All Journal Folia Medica Indonesiana Indonesian Journal of Tropical and Infectious Disease The Indonesian Biomedical Journal Paediatrica Indonesiana Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Indonesian Journal of Obstetrics and Gynecology (Majalah Obstetri dan Ginekologi Indonesia) Jurnal Kreativitas PKM Faletehan Health Journal Jurnal Respirasi (JR) Biomolecular and Health Science Journal Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Majalah Biomorfologi (Biomorphology Journal) Jurnal Layanan Masyarakat (Journal of Public Service) Cardiovascular and Cardiometabolic Journal (CCJ) Indian Journal of Forensic Medicine & Toxicology Neurona

Yetti Hernaningsih

Clinical Pathology Department, Dr. Soetomo Hospital Surabaya

Author-ID : 3289647

Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Social Sciences Other

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PLATELET RICH PLASMA PREPARATION PROTOCOLS: A PRELIMINARY STUDY Nugraha, Hans Kristian; Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Jusak
Indonesian Journal of Tropical and Infectious Disease Vol 3, No 2 (2012)
Publisher : Institute of Topical Disease

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.43 KB)

Currently, therapy with Platelet Rich Plasma (PRP) has been widely used and continues to grow for various clinical applications. Along with its development, there are various options in the method of obtaining PRP, either automatic or manual, while one of theÂ most reliable methods according to the literature is a double centrifugation method. The purpose of this research is to produce anoptimization of the double centrifugation method. This study used experimental data obtained by conducting a research at the ClinicalÂ Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Experiments were conducted on stored blood obtained from the blood bagÂ from Indonesian Red Crossand fresh blood from healthy donors with CPD anticoagulant. Results: PRP with optimum platelet countÂ could be made with sufficient personal laboratory skills and amounted to 4.11 times with the platelet count of 1.152 million using 1300Â rcf for 5 minutes for the first centrifugation, and 2300 rcf for 7 minutes for the second centrifugation.

UPAYA OPTIMASI PEMBUATAN PLASMA KAYA TROMBOSIT SEBAGAI PENGOBATAN SEL PUNCA Muljanti, Meiti; Hernaningsih, Yetti; Nugraha, Hans K; Nugraha, Jusak
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.480

Stem cells have a remarkable potential to act as a self renewal in the related system. The proliferation of stem cells can be stimulated by Platelet Rich Plasma (PRP) growth factor. PRP has a potential application for aesthetics, traumatology and maxillofacial surgery. The optimation method could be carried out by a double centrifugation, which is reliable and simple in producing an optimal PRP. The samples were obtained from healthy volunteers; 10 mL of blood was needed to produce 1 mL PRP. CBC test was then performed, in order to know the platelet count before and after double centrifugation. This method used four variations of speed and time. Four variations of speed and time were used to find the optimation result, which yield the highest platelet count and the highest PDGF level released after platelet activation. The measurement of Platelet Derived Growth Factor (PDGF-BB) level was done using ELISA method. Twenty first samples, resulted in a variation of platelet counts, the mean was 0.89 times. About 60% of platelet counts showed a decrease with the lowest value 0.01 times and 40% of the platelet counts showed an increase with the highest value 3.94 times. The study was repeated using “I” tubes, the highest increase of platelet count and PDGF-BB level was obtained by centrifugation at 900 g, 5 minutes duration, then followed by 1500 g, for 15 minutes. In this study protocol, the production of optimal PRP was not yet found, however it provided some important information. In this case, the influence of separation process and skill was more important than the centrifugation speed. The researchers suggested that the measurement of PDGF-BB level should be done immediately after PRP harvest.

Platelet Counts Analysis of Platelet-Poor Plasma (PPP) Produced by Several Centrifugation Techniques Pande Putu Rangga Raditya1 , Yetti Hernaningsih2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10559

Platelet-poor plasma (PPP) can be obtained by various centrifugation techniques. This study aims to analyze platelet count in PPP produced by three different centrifugation techniques. Samples came from a healthy adult who underwent medical check-up and had been given informed consent. A total of 2.7 mL of blood samples were collected using three citrated tubes. Samples were centrifuged by three different techniques to obtain PPP: 1,500 g for 15 minutes twice, 3,000 g for 15 minutes, and 3,260 g for 10 minutes. The platelet count of each PPP was examined using a hematology analyzer. All three centrifugation techniques produced <10,000/µL platelets in all PPP from 31 samples. The twice centrifugation of 1,500 g for 15 minutes produced a median number of platelets which was 1×103 /µL (0-3). The 3,000 g centrifugation for 15 minutes produced a median number of platelets which was 1×103 /µL (0-5). The 3,260 g centrifugation for 10 minutes produced a median number of platelets which was 2×103 /µL (0-5). A comparison of platelet count showed a significant difference (p<0.05) among the three centrifugation techniques. The three centrifugation techniques in this study were able to produce PPP. The centrifugation technique of 1,500 g for 15 minutes twice produced the lowest number of platelets.

The Effect of Storage and Time of Blood Specimen Examination of Plasma Prothrombin Time and Activated Partial Thromboplastin Time Stabilities Aditea Etnawati Putri1 , Yulia Nadar Indrasari2 , Yetti Hernaningsih2
Indian Journal of Forensic Medicine & Toxicology Vol. 14 No. 3 (2020): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v14i3.10664

his was an analytical study with cross sectional design. Collection of blood specimens was carried out by consecutive sampling during March-June 2019. Blood specimens in this study were healthy adults aged ?21 years who underwent general medical check-up or blood donors who met the inclusion criteria and signed informed consent. A total of 71 samples were stored at room temperature and temperature 4-8 °C, then PT (prothrombin time) and aPTT (activated partial thromboplastin time) checks were carried out at 0, 4, 12, and 24 hours. Data were analyzed by using Kolmogorov-Smirnov Test, paired t test and Wilcoxon Signed Rank Test. There were differences in the results of PT in the room temperature specimens and 4-8 °C at 4 hours, 12 hours, and 24 hours with p value <0.001. This was also found in the aPTT parameter, but for specimens at 4-8 °C at the 24th hour examination the results of changes in aPTT were not statistically significant with p values 0.062. Different PT and aPTT tests at the two storage areas and at different examination times obtained different and statistically significant results with p value <0.001. Examination of hemostasis physiology requires special attention, where pre-analytic plays an important role which can influence the overall test results. The aspects of time and storage on the stability of PT and aPTT specimens are illustrated in this study where significant differences were obtained with p values <0.001. Examination and storage of coagulation study specimens in accordance with CLSI recommendations will provide accurate results and accurately describe the state of the specimen according to the patient’s clinical condition.

The Impact Factors of Peripheral Diabetic Neuropathy Which is Along with Hyperglycemia, Hypercholesterolemia, and Hyperaggregation Citra Amaniah Anhar; Yetti Hernaningsih; Puspa Wardhani, Soebagijo Adi; Mohammad Saiful Ardhi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14696

Objective: The prevalence of T2DM case in 2018 has increased by 10.9%. The majority of T2DMpatients often ignore some of the most important conditions to experience complications, which iscomplications of PDN. This study aims to analyze the dominant factors in PDF patients with hyperglycemia,hypercholesterolemia, and hyperaggregation. Material and methods: This research is a case control studyconducted from April to October 2020. The sample is 85 patients who have been confirmed to have PDNby neurologists aged 40 - 65. Sampling was conducted at several hospitals in Surabaya with a purposivesampling method. Data analysis was conducted in three stages, namely univariate analysis, bivariateanalysis and multivariate analysis. Results: 85 PDN patients who experienced hyperglycemia amountedto 81%, hypercholesterolemia 81%, and hyperaggregation 67%. The dominant factor affecting the threeconditions (hyperglycemia, hypercholeterol, and hyperagregation) in this PDN patient was a history ofhypertension with a value of p = {(0.012); (0.007); (0.001)} and also a long period of suffering from T2DMwith p value = {(0.019); (0.025); (0.041)). Meanwhile, age, gender and regularity for treatment control werenot the dominant influencing factors. Conclusion: Suffered for a long time from T2DM and a history ofhypertension are the main determinants of peripheral diabetic neuropathy which is along by hyperglycemia,hypercholesterolemia, and hyperaggregation.

Comparison of PPT and APTT in Pre and Post-Hemodialysis Patients as the Heparin-Exposed Effect Yetti Hernaningsih; Widodo Widodo; Koko Aprianto
Folia Medica Indonesiana Vol. 55 No. 3 (2019): September
Publisher : Faculty of Medicine, Universitas Airlangga

Evaluations on Plasma Prothrombin Time (PPT) and Activated Partial Thromboplastin Time (APTT) are required in patients with Chronic Kidney Disease (CKD) stage V to determine the risk of bleeding after hemodialysis (HD) using heparin as the anticoagulant. This study aimed to compare the results of PPT and APTT in pre and post-hemodialysis patients with minimum dose of heparin. This was an observational-analytical study with cross-sectional design. The samples were collected in HD wards of Dr. Soetomo Hospital, Surabaya. There were 50 PPT and APTT samples collected from June to August 2017. The samples were evaluated using the tool CoaDATA 501. The examination of coagulation study was conducted in Clinical Pathology Laboratory of Dr. Soetomo Hospital, Surabaya. Paired t-test and Wilcoxon signed-rank test were performed in this study. In the 50 samples, pre-hemodialysis PPT ranged between 10.2-17.6 with the mean of 12.6±2.03 seconds, while for post-hemodialysis, the range was 10.1-20.9 with the mean of 13.41±2.43 seconds. Pre-hemodialysis APPT ranged between 19.5-75.2 with the mean of 30.32±10.43 seconds, while in post hemodialysis the range was 22.21-175 with the mean of 37.52±26.40 seconds. The results of PTT evaluation in pre and post-HD showed no significant difference (p=0.083), while those of APTT showed a significant difference (p=0.035 or p<0.05). Prolongation of APTT in post-HD is due to the use of heparin as an anticoagulant that increases PPT and APTT by inhibiting antithrombin III. HD procedures cause decreased activity of coagulation factors II, IX, X, XII leading to APTT prolongation in post-HD. A significant APTT prolongation was found in post-HD patients with CKD V.

Epidemiological Study of Clinical and Laboratory Profiles of Patients with Acute Lymphoblastic Leukemia at Dr. Soetomo Hospital Surabaya Kezia Warokka Putri; I Dewa Gede Ugrasena; Yetti Hernaningsih
Biomolecular and Health Science Journal Vol. 2 No. 1 (2019): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Introduction: Considering the high number of acute lymphoblastic leukemia (ALL) and it being the type of cancer with the highest fatality rate among the children, this study seeks to determine the epidemiological description of the clinical and laboratory profiles of patients with ALL.Methods: This research used a descriptive study by using medical data record of patients with ALL. The research variables were gender, age, leukemia history of the patient’s family, nutritional status, symptoms and signs, laboratory examination, ALL subtypes, risk factors, and result outcomes. All data presented descriptively.Results: From a total of 50 patients, 54 % of them were male aged 1,5 – 10 years old. 84% of the patients’ family had no medical record related to leukemia. 42% of the patient malnutrition. Pale (78%), fever (64%), pain (32%), hepatomegaly (38%), lymphadenopathy (28%), splenomegaly (26%), patients with anemia (82%), leukocytosis (38%), thrombocytopenia (54%). The highest types were ALL–LI (68%), SR-ALL (54%), and remission outcome reached 82%.Conclusion: Insidence higher in male, aged 1,5 – 10 years old, malnourished at the start of the diagnosis. Most of the patients’ family had no medical history of leukemia. Symptoms and medical signs mostly appeared were pale, fever, and bone/joint pain. The physical examination showed hepatomegaly, lymphadenopathy, and splenomegaly and laboratory first test showed the patients had anemia, leukocytosis, and thrombocytopenia.

Antimalarial Activity of Ethanol Extract of Noni Leaves (Morinda citrifolia) towards Parasitemia, Splenomegaly, and Hepatomegaly in Plasmodium berghei ANKA Infected Mice Putri Rahayu; Yetti Hernaningsih; Heny Arwati
Biomolecular and Health Science Journal Vol. 4 No. 1 (2021): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v4i1.26913

Introduction: Malaria is one of the infectious diseases found in tropical countries and sub-tropical countries. In 2016 there were an estimated 445,000 people died to malaria. Alternative medicine is needed, such as natural based ingredient. Morinda citrifolia or noni plant is a medicinal plant found in all parts of Indonesia which has many benefits, such as antibacterial, analgesic, anticancer, antioxidant, and anti-inflammatory. The aims of this study were to determine the antimalarial activity of ethanol extract of noni leaves and its effect on splenomegaly and hepatomegaly.Methods: Extract of noni leaves was prepared by maceration using ethanol solvent. In vivo experiments were conducted using Plasmodium berghei infected BALB/c mice treated with the doses of 100, 10, 1 mg/kg body weight(BW) orally of ethanolic extract of noni leaves. Then, the percentage of parasitemia was calculated from day 1 to day 4 after treatment and at the end of the test, mice were sacrificed then spleen and liver were collected. Results: The highest parasite growth was found in the group treated with noni leaves ethanol extract at a dose of 1 mg/kg WB and vice versa. Probit analysis resulted in ED50 was 0.882 mg/kg WB. Spearmen test showed there was no correlation between doses and the size of splenomegaly with p=0,2 and between doses and the size of hepatomegaly with p=0,6.Conclusion: Ethanol extract of noni leaves possessed antimalaria activity and there was no correlation between doses of extract and t he splenomegaly and hepatomegaly.

COMPARISON OF TOTAL ANTIOXIDANT CAPACITY (TAC)’S PATIENTS OF CHRONIC KIDNEY DISEASE UNDERGOING HEMODIALYSIS AND NON-HEMODIALYSIS Zaky Firmawan El-Hakim; Mochammad Thaha; Yetti Hernaningsih
Majalah Biomorfologi Vol. 30 No. 2 (2020): MAJALAH BIOMORFOLOGI
Publisher : Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/mbiom.v30i2.2020.52-58

Background: Total antioxidant capacity (TAC) is one of the biomarkers to determine a person's health condition through antioxidant and oxidant activity or oxidative stress so that it can help in determining the therapy needed. However, currently, TAC levels in patients with chronic kidney disease (CKD) undergoing hemodialysis (HD) and non-hemodialysis (non-HD) are still controversial, so further research is needed. Objective: To analyze the comparison of TAC in HD and non-HD CKD patient. Materials and Methods: This was an analytical cross-sectional study with clinical observation in CKD patient. A total of 71 CKD patients consisting of 28 CKD HD patients and 43 non-HD CKD patients were enrolled in this study. Data about the characteristics of the subjects were taken using the anamnesis method, while data about TAC were taken using the colorimetric method with COBAS C-501 0837-19 series and 0835-13 series to measure TAC levels in the serum of CKD patients. The data that has been obtained were analyzed using independent sample t-tests. Results: TAC levels were significantly higher in CKD patients undergoing HD compared to non-HD CKD patients (p <0.05). There are data on albumin as an antioxidant and MDA as an oxidative stress biomarker that affects TAC levels. Conclusion: TAC levels found to be higher in hemodialysis CKD patient compared to non-hemodialysis CKD patient.

DIFFERENTIATION T LYMPHOCYTE CELLS EXPRESSING INTERLEUKIN-17 PERCENTAGE ON HEALTHY PERSON AND ADULT ACUTE MYELOID LEUKEMIA PATIENT Elvan Dwi Widyadi; Yetti Hernaningsih; Endang Retnowati; Ugroseno Ugroseno; Ryzky Widi Atmaja
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 25, No 2 (2019)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v25i2.1383

Acute Mieloid Leukemia (AML) is a hematologic cause of cancer deaths of 1.2% including a relatively rare disease but by the end of the decade there is an increase in the number of new cases. The immune system in AML is caused by gene mutations giving immunosuppressive effects so that the immune system will be inhibited in eliminating leukemia cells. The immune response of tumors is important to determine the prognosis, development of new cancer immunotherapy as well. One of the subset of lymphocytes T is gdT lymphocyte cell with innate nature, but until now no information is required about gdT cell profile in AML patients. gdT cells have properties as antitumors played by Interferon production g (INF g), and the nature of protumor by interleukin 17 (IL-17). The percentage of lymphocyte T (CD3 +) of AML patients and healthy people did not differ (p = 0.528), indicating, not being activated for proliferation. gdT Lymphocyte cells percentage in healthy people by race, genetic and exposure to the surrounding environment such as infection. Percentage of gdT lymphocyte of AML patients and healthy people was not different from (p = 0.694), showed an immune response by gdT cells Unefected to proliferate. The percentage of gdT llimfocytes expressing the interleukin 17 (gdT17 cells)in patients AML and healthy people did not differ significantly (p = 0.436), this indicates inhibited proliferation.

Co-Authors -, Regina Rania Cahya Kusumaningrum Adil Dinata Simangunsong Agil Saputra Alfino Validita Sidiq Alifadiningrat, Dianira Hanum Febia Almira, Naufalia Ghina Alpha Fardah Athiyyah Amalia, Yustisia Anang Endaryanto Andi Cahyadi Andrianto Andy Cahyadi Ardhi, Mohammad Saiful Arifa Mustika Arifa Mustika Arifoel Hajat Armayani, Erawati Aryati Aryati Aulia, Fauqa Arinil Awalia Awaliah Baiq Nasha Islaeli Bintoro, Siprianus Ugroseno Yudho Budi Utomo Chelssi Gloria Tessari Cita Rosita Sigit Prakoeswa Citra Amaniah Anhar Danny Meganingdyah Primartati Deasy Fetarayani Desty Indah Sari Diah Puspitarini Dwiyanti Puspitasari, Dwiyanti DYAH FAUZIAH, DYAH Elvan Dwi Widyadi Elvan Dwi Widyadi Endang Retnowati Endang Retnowati Endang Retnowati Erawati Armayani Esti Rohani Farida Nur’Aini Fauqa Arinil Aulia Fita Triastuti Gatot Soegiarto Hanik Badriyah Hidayati,* Mohammad Hasan Machfoed,* Kuntoro,** Soetojo,*** Budi Santoso,**** Suroto,***** Budi Utomo****** Hans Kristian Nugraha, Hans Kristian Hartono Kahar, Hartono Hasan, Helmia Heny Arwati I Dewa Gede Ugrasena Indrasari, Yulia Indrasari, Yulia Nadar Indriastuti, Endah INGRID SURYANTI SURONO Jusak Nugraha Juwita, Syntia Tanu Kezia Warokka Putri Koko Aprianto Kusuma, Edward Putra Lazuwardi, Rasya Azka Lefi, Achmad Meiti Muljanti, Meiti Mia Ratwita A Mia Ratwita Andarsini Mochammad Reza Desianto MOCHAMMAD THAHA Musholli Himmatun Nabilah Nadya Rinda Eka Rana Nanda Aulya Ramadhan Narazah Mohd Yusoff Nastasya Nunki Nathalya Dwi Kartikasari Ni Made Rindra Hermawathi Notopuro, Paulus Budiono Novi Ersanto Nugraha, Hans K Nunki, Nastasya Nur‘ Aini, Farida Puspa Wardhani Puspa Wardhani, Soebagijo Adi Puspitasari, Yessy Putri Rahayu Putri, Aditea Etawati Rahmi Rusanti Rahmi Rusanti, Rahmi RINI RINI Rinta Prasetiyanti Romadhon, Pradana Zaky Rusli, Musofa Ryzky Widi Atmaja Ryzky Widi Atmaja S.Pd. M Kes I Ketut Sudiana . Sellynastiti, Sarah Triwinar Shinta Lungit Ambaringrum Sidarti Soehita Siti Khaerunnisa Soeprapto Ma’at Sri Ratna Dwiningsih Suprapto Ma'at Ma'at Suprapto Ma’at Syntia TJ Tanzilia, May Fanny Teguh Satrio Thengkano, Irwanto Ugroseno Ugroseno Uli Mas'uliyah Indarwati Ummi Maimunah Veithzal Rivai Zainal Wardhani, Puspa Widaninggar Rahma Putri Widodo Widodo Widodo Widodo Widodo Widodo Wulyansari Wulyansari Yanuari Primariawan, Relly Yosua Butar Butar Yulia Nadar Indrasari Yusuf, Moch Zaky Firmawan El-Hakim

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