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All Journal JBIO: jurnal biosains (the journal of biosciences)
Nasution, Mhd Yusuf
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

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FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 10, No 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 9 No. 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
TESTING THE ACTIVITY OF KETUL LEAF (Bidens pilosa L.) ETHANOL EXTRACT IN INHIBITING THE GROWTH OF Escherihia coli BACTERIA Munthe, Melia Ivana; Nasution, Mhd Yusuf; Pulungan, Ahmad Shafwan S
JURNAL BIOSAINS Vol. 10 No. 2 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i2.52933

Abstract

Daun ketul (Bidens pilosa L.) memiliki senyawa metabolit sekunder yang dimanfaatkan masyarakat dalam mengobati penyakit diare. Daun ketul memiliki senyawa metabolit sekunder antara lain terpenoid, alkaloid, flavonoid, dan fenool yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun ketul (B. pilosa L.) dalam menghambat pertumbuhan bakteri Escherichia coli. Penelitian ini menggunakan metode difusi cakram yang terdiri dari 5 variasi konsentrasi yaitu 30%, 40%,50%,60%,70%, dengan 4 kali pengulangan. Data hasil zona hambat  dianalisis dengan uji statistik One Way Anova.  Hasil penelitian menunjukkan ekstrak daun ketul (Bidens pilosa L.) memiliki aktivitas antibakteri  terhadap bakteri Escherichia coli. Semakin luas zona bening yang terbentuk maka semakin kuat senyawa aktif dalam menghambat pertumbuhan bakteri. Berdasarkan hasil uji lanjutan Duncan diperoleh bahwa tidak ada perbedaan secara nyata dari setiap perlakuan konsentrasi dalam menghambat bakteri Escherichia coli.
Co-Authors Gultom, Endang Sulistyarini Habsari, Lilis Munthe, Melia Ivana Pulungan, Ahmad Shafwan S Rangkuti, Marlinda Nilan Sari