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CHARACTERIZATION OF BACTERIA Salmonella sp. ON BROILER CHICKEN MEAT MARKETED AT SUKARAMAI MARKET, MEDAN CITY Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S; Silaban, Friend
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.49964

Abstract

One effort to meet high protein needs is obtained from chicken meat. Chicken meat is a food source that is composed of various nutritional values in balanced proportions. There is still doubt about Boiler Chicken meat being sold for sale because it is suspected that there is contamination with pathogenic microbes that can cause disease in humans. There is a risk that broiler chicken meat will be contaminated due to poor market sanitation. The aim of this research was to determine the bacterial contamination of Salmonella sp. in broiler chicken meat and the number of Salmonella sp bacteria. Which is found in samples of broiler chicken meat marketed at the Sukaramai Market, Medan City. This research method is a quantitative descriptive approach. This research was carried out at the Regional Health Laboratory of North Sumatra Province in May-June 2023. In this study, sample culture was carried out by streaking on Salmonella Shigella Agar (SSA) media, while the Total Plate Count (TPC) test for Salmonella sp. using pour plate media. The results showed that all 9 samples contained Salmonella sp. with the amount of bacterial contamination, namely A1 3.0 × 10-4, A2 3.9 × 10-4, A3 4.7 × 10-4, B1 4.0 × 10-4, B2 5.3 × 10-4, B3 6.0×10-4, C1 4.5×10-4, C2 6.8×10-4, C3 7.7×10-4. Based on the National Chicken Meat Standardization Agency ICS No. 67-120-20. In 2009, the maximum limit for microbial contamination and the maximum limit for residues in food ingredients of animal origin must not contain Salmonella sp.
FIG ENDOPHYTIC BACTERIA PRODUCE ENZYMES AMYLASE, LIPASE, PROOTEASE, CELLULASE Habsari, Lilis; Gultom, Endang Sulistyarini; Nasution, Mhd Yusuf; Rangkuti, Marlinda Nilan Sari; Pulungan, Ahmad Shafwan S
JBIO: jurnal biosains (the journal of biosciences) Vol 9, No 3 (2023): JBIO : Jurnal Biosains (The Journal of Biosciences)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v9i3.52820

Abstract

The use of enzymes in Indonesia is increasing every year, so action is needed to harmonize the production and needs of enzymes in Indonesia. Enzymes can be produced from various sources such as plants, animals and microorganisms. The advantage of utilizing microorganisms in enzyme production is that they can be produced in large quantities with a shorter time and can be produced continuously. One of the sources of bacterial screening to produce enzymes is figs. This study aims to look at the extracellular enzyme activity of fig endophytic bacteria and identify endophytic bacteria capable of producing extracellular enzymes such as proteases, amylase, cellulase and lipase. Isolation and purification of bacteria using NA media for 24 hours. Test of extracellular enzyme activity using NA media enriched with amylum, tween 80, skim milk, CMC. Biochemical tests are performed to identify endophytic bacteria of figs producing extracellular enzymes. The results of the study were 21 isolates of fig endophytic bacteria, as many as 14 isolates were able to produce extracellular enzymes. The identification results of fig endophytic bacteria producing extracellular enzymes obtained 8 isolates suspected to be from the genus Bacillus, 3 isolates suspected to be from the genus Micrococcus, 1 isolate suspected to be from the genus Cellulomonas, 2 isolates suspected to be from the genus Acetobacter
Pengaruh Pemberian Pupuk Hayati Mikoriza Terhadap Pertumbuhan Tanaman Tomat (Lycopersicum esculentum Mill) Pulungan, Ahmad Shafwan S.; Rangkuti, Marlinda Nilan Sari; Rahmi, Nisrina Afiqah
Jurnal Ekonomi Pertanian dan Agribisnis Vol. 1 No. 1 (2023): Juli - Desember
Publisher : CV.ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62379/jepag.v1i1.1295

Abstract

Mycorrhiza is a type of biological fertilizer that can improve soil health, environmental protection, soil nutrient status and agricultural yields. The demand for tomatoes continues to increase every year along with population growth and in 2021. In order to meet the need for tomato consumption every year, various efforts are being made to continue to increase production through the use of minimal and environmentally friendly output such as the use of mycorrhiza biological fertilizer. This research aims to determine the effect of the use and dosage of mycorrhizal fertilizer on the growth of tomato plants (Lycopersicum esculentum Mill). This research was carried out on Jln Rela, Gg. Danau Toba, No. 16. starting from 10 September 2023 - 11 November 2023. Mycorrhizal fertilizer was given in 2 treatments, namely 5 gr and 10 gr with 2 repetitions each and control (no mycorrhizal fertilizer was given). Providing 10g of mycorrhizal biofertilizer/plant provides the best growth and results for tomato plants with the parameters of plant height and number of leaves.
Pengaruh Pemberian Pupuk Hayati Mikoriza Terhadap Pertumbuhan Tanaman Bawang Daun (Allium fistulosum L.) S. Pulungan, Ahmad Shafwan; Rangkuti, Marlinda Nilan Sari; Malau, Elfrida; Finanta, Olyfiah; Atika, Zuhairah
Jurnal Ekonomi Pertanian dan Agribisnis Vol. 1 No. 1 (2023): Juli - Desember
Publisher : CV.ITTC INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62379/jepag.v1i1.1298

Abstract

Leeks (Allium fistulosum L.) are a type of vegetable plant in the genus Allium which is widely cultivated in Indonesia. Scallions are also a plant that is often used as an additional spice in cooking. In general, the production of leek plants (Allium fistulosum L.) really requires a good level of soil fertility where the level of soil fertility is also determined by the microorganisms contained therein. For the living environment such as soil, the presence of microorganisms can determine the level of soil fertility and improve soil conditions. The aim of this research was to examine the effect of mycorrhizal biofertilizer on the growth of leek (Allium fistulosum L.) and determine the effect of mycorrhizal biofertilizer on the growth of leek (Allium fistulosum L.). The method used in the research was a laboratory experiment which was carried out directly by researchers at the Microbiology Laboratory, Medan State University. The results of this research were that the treatment of mycorrhizal fertilizer on leeks had 2 parameters, namely plant height and number of leaves, which had an optimal effect. Based on the average yield data from the yield table, the application of a single mycorrhizal fertilizer to red spinach plants really had a big influence on its growth, where the height and number of leaves obtained each week increased 2 times more.
GC-MS ANALYSIS OF SECONDARY METABOLITES OF SENGGANI LEAVES (Melastoma malabatrichum L.) Nasution, Muhammad Yusuf; Rangkuti, Marlinda Nilan Sari; Situmorang, Nurbaity
JBIO: jurnal biosains (the journal of biosciences) Vol. 10 No. 3 (2024): JBIO : JURNAL BIOSAINS (THE JOURNAL OF BIOSCIENCES)
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v10i3.62912

Abstract

Traditional medicinal plants have long been used and trusted by the community to treat various diseases. The senggani plant is useful for reducing fever (antispiretic), pain reliever (analgesic), urine laxative (diuretic), treating vaginal discharge (leukorrhea), and as a medicine for various types of wounds. This research was conducted using extracts of Melastoma malabatricum (L.) leaves extracted using methanol as a solvent. GC-MS analysis of secondary metabolites of senggani leaves (Melastoma malabatrichum L.) was carried out as a first step to determine the content of active secondary metabolite compounds contained in senggani leaves.The results of this study show that 8 peaks were observed during maximum run time of 40 min. The results revealed that, compounds such as 3-Hexadecyloxycarbonyl-5-(2-hydroxyethyl)-4-methylimidazolium, Melezitose, Thiosulfuric acid, Neophytadiene, 9-Icosyne, Phytol, Hexadecanoic acid dan 9-Octadecenoic acid (Z) were present in the methanolic extract of Melastoma malabatrichum (L.) which can mostly contribute to several therapeutic activities such as antimicrobial.
ANALYSIS OF STUDENTS' LEARNING DIFFICULTIES ON PROTISTA MATERIAL CLASS X MIA PANCASILA EDUCATION FOUNDATION SAWIT SEBRANG 2024/2025 Sari, Widya; Rangkuti, Marlinda Nilan Sari
Jurnal Pelita Pendidikan Vol. 13 No. 2 (2025): JURNAL PELITA PENDIDIKAN
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aims to determine students' learning difficulties in the material protista based on learning outcome indicators and cognitive aspects and factors causing learning difficulties of class X students of SMA Yayasan Pendidikan Pancasila in the 2024/2025 Academic Year. This type of research is descriptive-quantitative research using several data collection techniques, namely questionnaires and learning outcome tests. The most dominant external factor causing students' difficulties in learning biology protista material in class X MIA Yayasan Pendidikan Pancasila is the Family Environment factor in the Very High Difficulty category with an overall average of 91.96%. Meanwhile, the most dominant internal factor causing students' difficulties in learning biology protista material in class X MIA Swab Yayasan Pendidikan Pancasila is the Intelligence factor in the Very High Difficulty category with an overall average of 92.53%.
Isolation And Characterization Of Yeast And Mold From The Khamir Kapang Number Test (AKK) On Lemang And Air Pollution Test In The Original Lemang Production Room Of Tebing Tinggi City Alifka, Carmenita; Rangkuti, Marlinda Nilan Sari
BEST Journal (Biology Education, Sains and Technology) Vol 8, No 1 (2025): Juni 2025
Publisher : Program Studi Pendidikan Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30743/best.v8i1.12137

Abstract

Penelitian ini bertujuan untuk mengetahui nilai Angka Khamir Kapang (AKK) pada lemang mengetahui nilai cemaran udara pada ruang tempat produksi lemang dan mengetahui genus dari khamir dan kapang yang diisolasi dan dikarakterisasi pada lemang dan ruang tempat produksi lemang asli Kota Tebing Tinggi. Penelitian ini dilakukan dengan metode eksperimental yaitu menguji 3 sampel lemang dan ruang tempat produksi lemang yaitu lemang 01, lemang 02 dan lemang 03. Hasil penelitian menunjukkan bahwa pada 3 sampel lemang mempunyai nilai AKK yang masih dalam batas aman untuk dikonsumsi yaitu kurang dari 1×105 dan 3 sampel udara ruang tempat produksi lemang masih dikategorikan batas aman ruang terkontaminasi, setelah dilakukan karakterisasi morfologi pada lemang 01 ditemukan genus Penicillium, pada lemang 02 ditemukan genus Candida dan Penicillium, dan pada lemang 03 ditemukan genus Aspergillus dan Penicillium. Sedangkan, pada ruang tempat produksi lemang 01 dan lemang 02 ditemukan genus Aspergillus dan Penicillium, sedangkan pada lemang 03 ditemukan genus Aspergillus, Alternaria, dan Trichoderma