Article Per Year (5 Year)
p-Index From 2020 - 2025
0.444
P-Index
This Author published in this journals
All Journal Jurnal Kedokteran Brawijaya Medical and Health Journal
Nitihapsari, Galuh Yulieta
Unknown Affiliation
Medicine & Pharmacology

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Immunomodulatory Effect of Nigella sativa Extract through the Improvement of IL-1β Level in Balb-c Mice Infected by Methicillin-resistant Staphylococcus aureus Nitihapsari, Galuh Yulieta; S, Octavia Permata; Ferine, Miko
Jurnal Kedokteran Brawijaya Vol 31, No 2 (2020)
Publisher : Fakultas Kedokteran Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jkb.2020.031.02.3

Abstract

Nigella sativa (NS) has been used for many years as an anti-bacterial herbal treatment, such as Methicillin-resistant Staphylococcus aureus, but the mechanism in bacterial elimination is still unknown. IL-1β is a proinflammatory cytokine that helps the immune system eliminate microbes when it enters the body. The purpose of this study was to prove the immunomodulatory effects of Nigella sativa extract through increased IL-1β in dealing with MRSA infections. The design of this study was post-test only control group design using 25 male Balb-c mice infected with MRSA and randomly divided into five groups. Group K was infected with MRSA without being treated. All treatment groups were given NS extracts in different doses for 7 days before being infected with MRSA. P1 was given N. sativa extract 0.05ml, P2 (was given NS extract 0.25ml, P3 was given 0.5ml NS extract, and P4 was given 0.75ml NS. On the 8th day, blood was taken from the retroorbital plexus for IL-1β level examination using ELISA. Statistical tests were done using the One Way ANOVA test. The results showed the mean of IL-1β levels in group K was 11.65ng/L, P1 was 14.07ng/L, P2 was 16.66ng/L, P3 was 18.54ng/L, and P4 was 19.49ng/L which showed an increase in IL-1β levels along with the addition of black cumin dose although there was no difference between groups (p=0.578: ANOVA test). Nigella sativa extract is not proven effective in increasing IL-1β levels of Balb-c mice infected with MRSA.
Comparative Antibacterial Effects of Honey Bee Propolis on Gram Negative Bacteria Escherichia coli and Gram Positive Bacteria Staphylococcus aureus Hestiyani, Rani Afifah Nur; Handini, Tri Okmawati; Nitihapsari, Galuh Yulieta; Ning Widhi, Anriani Puspita Karunia; Wahyudin, Wahyudin
Medical and Health Journal Vol 5 No 1 (2025): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2025.5.1.15814

Abstract

Antimicrobial resistance in pathogens such as Escherichia coli and Staphylococcus aureus poses an urgent global health challenge. Honeybee propolis is known for its promising antibacterial potential.This study aimed to compare the antibacterial effectiveness of propolis against E. coli (Gram-negative) and S. aureus (Gram-positive) using the in vitro disk diffusion method. Propolis was tested at concentrations of 6.25%, 12.5%, 25%, 50%, and 100% against both bacterial species. Disk diffusion assays were conducted on Mueller Hinton Agar, and inhibition zones were measured using a vernier caliper. Data were analyzed using one-way ANOVA. The results showed the Inhibition zone diameters increased with higher propolis concentrations. At 100% concentration, S. aureus showed a 15 mm inhibition zone, while E. coli showed only 7 mm. One-way ANOVA indicated significant differences (p < 0.05) in both bacteria. These findings indicate that propolis exhibits antibacterial activity against both S. aureus and E. coli, with greater effectiveness against Gram-positive S. aureus. These findings support the potential use of propolis as a natural antibacterial agent, particularly for Gram-positive infections.
Co-Authors Handini, Tri Okmawati Miko Ferine Ning Widhi, Anriani Puspita Karunia S, Octavia Permata Wahyudin Wahyudin