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All Journal Mandala of Health : A Scientific Journal Medical and Health Journal Linggamas: Jurnal Pengabdian Masyarakat
Ning Widhi, Anriani Puspita Karunia
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AKTIVITAS ANTIBIOFILM PROPOLIS LEBAH MADU TERHADAP BAKTERI Pseudomonas aeruginosa Purnama, Fadila Milenia; Nur Hestiyani, Rani Afifah; Ning Widhi, Anriani Puspita Karunia; Wahyudin, Wahyudin
Mandala Of Health Vol 17 No 2 (2024): Mandala of Health: a Scientific Journal
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mandala.2024.17.2.9585

Abstract

Background: The number deaths of the infection effect from the bacterium Pseudomonas aeruginosa reaches 50% depending on the type of infection. The P.aeruginosa can be found in various environments, one of which is the hospital, with an incidence around 10-15%. P.aeruginosa is one of the bacteria that is difficult to treat because of its ability to form biofilm. Propolis is a natural resin containing flavonoid, fenol and terpenoid which used as antibiofilm. This study aims to examine the antibiofilm activity of propolis in inhibiting the formation of P. aeruginosa biofilm. Methods: This study is an in vitro laboratory experimental with a post-test only control group design. The number of samples was determined by the federer formula, where the samples in this study were 4 per groups. The propolis dissolved using aquades with the result the concentration became 12.5%, 6.25%, 3.125%, 1.56%, 0.78%. The proces of of inhibition of biofilm formation was measured using a microtiter plate assay with a wavelength of 620 nm. Results: Propolis can inhibit biofilm formation by 19.67% at a concentration of 0.78%. However, the proces cannot inhibit biofilm formation at other high concentrations because of the high viscosity of propolis, and the presence of other propolis ingredients which may be able to initiate biofilm formation. Conclusion: Propolis has antibiofilm activity against P.aeruginosa bacteria at a concentration of 0.78%.
Comparative Antibacterial Effects of Honey Bee Propolis on Gram Negative Bacteria Escherichia coli and Gram Positive Bacteria Staphylococcus aureus Hestiyani, Rani Afifah Nur; Handini, Tri Okmawati; Nitihapsari, Galuh Yulieta; Ning Widhi, Anriani Puspita Karunia; Wahyudin, Wahyudin
Medical and Health Journal Vol 5 No 1 (2025): August
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mhj.2025.5.1.15814

Abstract

Antimicrobial resistance in pathogens such as Escherichia coli and Staphylococcus aureus poses an urgent global health challenge. Honeybee propolis is known for its promising antibacterial potential.This study aimed to compare the antibacterial effectiveness of propolis against E. coli (Gram-negative) and S. aureus (Gram-positive) using the in vitro disk diffusion method. Propolis was tested at concentrations of 6.25%, 12.5%, 25%, 50%, and 100% against both bacterial species. Disk diffusion assays were conducted on Mueller Hinton Agar, and inhibition zones were measured using a vernier caliper. Data were analyzed using one-way ANOVA. The results showed the Inhibition zone diameters increased with higher propolis concentrations. At 100% concentration, S. aureus showed a 15 mm inhibition zone, while E. coli showed only 7 mm. One-way ANOVA indicated significant differences (p < 0.05) in both bacteria. These findings indicate that propolis exhibits antibacterial activity against both S. aureus and E. coli, with greater effectiveness against Gram-positive S. aureus. These findings support the potential use of propolis as a natural antibacterial agent, particularly for Gram-positive infections.
Peningkatan Pengetahuan dan Keterampilan Pengelolaan Limbah Infeksius Rumah Tangga dari Penanganan Covid-19 di Desa Sokaraja Tengah Munfiah, Siti; Wibowo, Yudhi; Dwi Laksana, Agung Saprasetya; Sari, Octavia Permata; Ning Widhi, Anriani Puspita Karunia
Linggamas: Jurnal Pengabdian Masyarakat Vol 1 No 1 (2023): Linggamas: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Kedokteran Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.linggamas.2023.1.1.9394

Abstract

Meningkatnya kasus COVID-19 menyebabkan semakin banyak jumlah pasien COVID-19 yang melakukan isolasi mandiri. Kondisi demikian menyebabkan timbulan limbah infeksius dari rumah tangga semakin meningkat. Limbah infeksius apabila tidak dikelola dengan baik, dapat menjadi media penyebaran virus COVID-19. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan keterampilan masyarakat dalam mengelola limbah infeksius rumah tangga. Metode pengabdian kepada masyarakat dilakukan dengan sosialisasi, pelatihan pembuatan disinfektan, pelatihan pengelolaan limbah infeksius rumah tangga dan penerapan teknologi tepat guna berupa sprayer disinfeksi dan alat pelindung diri bagi petugas pengelola sampah. Peserta pengabdian kepada masyarakat adalah satgas COVID-19, tokoh masyarakat, perangkat desa, PKK, karang taruna, kader kesehatan dan petugas pengelola limbah infeksius rumah tangga. Pengelolaan limbah infeksius rumah tangga berpedoman pada Surat Edaran Menteri Lingkungan Hidup dan Kehutanan Nomor SE.2/MENLHK/PSLB3/PLB.3/2020 dan Nomor SE.3/MENLHK/PSLB.3/3/2021. Pengurangan timbulan sampah dapat dilakukan dengan menggunakan masker guna ulang dari bahan kain tiga lapis. Apabila menggunakan masker sekali pakai maka sebelum dibuang ke tempat sampah dilakukan disinfeksi dan merusak masker dengan cara dirobek atau digunting. Hasil evaluasi diperoleh peningkatan pengetahuan yang bermakna sebelum dan sesudah pelaksanaan kegiatan. Peserta pengabdian kepada masyarakat diharapkan untuk menerapkan dan menyebarluaskan informasi kepada keluarga dan masyarakat luas.
Co-Authors Dwi Laksana, Agung Saprasetya Handini, Tri Okmawati Nitihapsari, Galuh Yulieta Octavia Permata Sari, Octavia Permata Purnama, Fadila Milenia Siti Munfiah Wahyudin Wahyudin Wahyudin Wahyudin Yudhi Wibowo, Yudhi