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All Journal Jurnal Sains Dasar Jurnal Teknologi Pertanian ASEAN Journal of Community Engagement
Rizal, Wahyu Anggo
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KARAKTERISTIK DAN AKTIVITAS ANTIBAKTERI ASAP CAIR DARI BIOMASSA KAYU PUTIH (MELALEUCA LEUCADENDRA) DAN KAYU JATI (TECTONA GRANDIS) Suryani, Ria; Rizal, Wahyu Anggo; Pratiwi, Diah; Prasetyo, Dwi Joko
Jurnal Teknologi Pertanian Vol 21, No 2 (2020)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.54 KB) | DOI: 10.21776/ub.jtp.2020.021.02.4

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengetahui karakteristik serta aktivitas antibakteri asap cair yang berasal dari biomassa kayu putih (Melaleuca leucadendra) dan kayu jati (Tectona grandis). Asap cair dalam penelitian ini diperoleh melalui kondensasi asap yang dihasilkan dari pirolisis biomassa kayu putih dan kayu jati pada suhu 250°C selama 8 jam. Asap cair yang terbentuk dari kedua biomassa memiliki karakteristik warna cokelat transparan, pH 2,8 (asam), dan masih memiliki bahan padatan terapung. Hasil uji antibakteri asap cair dari kayu putih dan kayu jati dengan metode difusi agar terhadap tiga (3) isolat bakteri patogen yaitu Escherichia coli FNCC 194, Staphylococcus aureus FNCC 0047, dan Pseudomonas aeruginosa FNCC 0156 menunjukkan hasil positif dengan terbentuknya zona jernih di sekeliling cakram. Hasil uji antibakteri asap cair dari biomassa kayu jati terhadap Staphylococcus aureus FNCC 0047 menunjukkan perbedaan yang signifikan dibandingkan dengan kontrol positif, yaitu Ampicillin 10 mcg. Karakterisasi fisik dan analisis GC-MS digunakan untuk mengetahui komposisi asap cair yang berasal dari dua biomassa yang berbeda. Diketahui bahwa acetic acid merupakan komponen utama yang ditemukan pada kedua sampel, yaitu sebesar 45,35% pada asap cair dari biomassa kayu putih dan 25,35% pada asap cair dari biomassa kayu jati. Selain acetic acid, kandungan fenol juga ditemukan pada kedua asap cair. Asap cair dari biomassa kayu putih mengandung fenol sebesar 6.53%, sedangkan asap cair dari biomassa kayu jati mengandung fenol sebesar 11.19%. Tingginya kandungan fenol yang dimiliki asap cair dari biomassa kayu jati diduga turut berpengaruh terhadap kemampuannya dalam menghambat pertumbuhan bakteri.Kata Kunci: Difusi Agar; Kondensasi; Pirolisis   ABSTRACT  This study aims to determine the characteristics and antibacterial activity of liquid smoke derived from the biomass of eucalyptus (Melaleuca leucadendra) and teak wood (Tectona grandis). Liquid smoke in this study was obtained through condensation of smoke produced from pyrolysis of eucalyptus and teak wood biomass at a temperature of 250°C for 8 hours. Liquid smoke formed from both biomass has the characteristics of transparent brown color, pH 2,8 (acid), and still has a floating solid materials. Antibacterial assay results of liquid smoke from eucalyptus and teak wood with diffusion method to three (3) isolates of pathogenic bacteria namely Escherichia coli FNCC 194, Staphylococcus aureus FNCC 0047, and Pseudomonas aeruginosa FNCC 0156 showed positive results with the formation of clear zones around the discs. Antibacterial liquid smoke assay results from teak wood biomass against Staphylococcus aureus FNCC 0047 showed a significant difference compared to positive control, Ampicillin 10 mcg. Physical characterization and GC-MS analysis were used to determine the composition of liquid smoke originating from two different biomass. It is known that acetic acid is the main component found in both samples, which is 45.35% in liquid smoke from eucalyptus biomass and 25.35% in liquid smoke from teak biomass. In addition to acetic acid, phenol content is also found in both liquid smoke. Liquid smoke from eucalyptus biomass contains phenol of 6.53%, while liquid smoke from teak biomass contains phenol of 11.19%. The high phenol content of liquid smoke from teak biomass is thought to have an effect on its ability to inhibit bacterial growth.Keywords : Agar Diffusion; Condensation; Pyrolysis
Stingles Bee Propolis Extract as An Active Ingredient in Antibacterial Ointment Formula Arrijal, Nail Zufar; Fauziyyah, Fadhilah Fida; Sugiarto, Eko; Hayati, Septi Nur; Rizal, Wahyu Anggo
Jurnal Sains Dasar Vol. 14 No. 1 (2025): April 2025
Publisher : Faculty of Mathematics and Natural Science, Universitas Negeri Yogyakarta

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Abstract

Stingless bees, also known as kelulut or klanceng (in local languages), are now widely cultivated in Indonesia. Stingless bees produce honey and propolis, which is the residue from honey extraction and is more abundant than honey itself. In this study, the processing of honey sacs or propolis into active ingredients for antibacterial ointments was optimized, specifically to address skin diseases caused by the bacteria Staphylococcus aureus and Streptococcus mutans. Propolis was extracted using aqua dest and 96% ethanol and optimized using three methods, namely sonication, maceration, and decoction, and antibacterial testing was carried out on S. aureus and S. mutans test bacteria. Antibacterial testing was carried out on the extracts using the diffusion method and the microdilution method. The results of the testing showed that propolis extract was most effectively extracted with water solvent, which had a bacterial inhibition concentration of 1,000–8,000 μg/mL. Water-based propolis extract can be used as a raw material for antibacterial ointments with a usage concentration of 5–15% and has been proven effective in inhibiting bacterial growth as observed using a Scanning Electron Microscope (SEM).
Coconut Shell Waste Treatment Technology for A Sustainable Waste Utilization: A Case Study of the SMEs in Bohol Village, Indonesia Rizal, Wahyu Anggo; Suryani, Ria; Maryana, Roni; Prasetyo, Dwi Joko; Pratiwi, Diah; Ratnawati, Yuli Ary; Ariani, Dini; Suwanto, Andri
ASEAN Journal of Community Engagement Vol. 6, No. 2
Publisher : UI Scholars Hub

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Abstract

This project focused on the introduction of liquid smoke and bio briquette manufacturing technology to small and medium enterprises (SMEs) located in Bohol Village, Gunungkidul, Indonesia through technology transfer, training, and mentoring. These SMEs are engaged in the food business with the main product jenang ketan, a traditional food from Gunungkidul, especially in Rongkop District, and very popular among communities around and outside Gunungkidul. The traditional preparation of jenang ketan produces several by-products in the form of waste, which is dominated by coconut shell biomass. The project’s technology transfer was carried out through the procurement of a series of pyrolysis reactors, distillation reactors, grinder machines, mixer machines, and briquette press machines for SMEs. Afterwards, the training was performed through teaching and practice of SMEs with a focus on the pyrolysis process, bio-briquette process, distillation, application of liquid smoke and bio-briquette, and marketing techniques. The activity was conducted within a period of one-year; continuous mentoring was carried out for the SMEs through consultation and direct field evaluation. The result of the project revealed an increase in the knowledge of SME personnel in the processing of coconut shells waste to produce liquid smoke and bio-briquette and the capability of SMEs to produce their own liquid smoke and briquette.
Co-Authors Ariani, Dini Arrijal, Nail Zufar Diah Pratiwi Eko Sugiarto Fauziyyah, Fadhilah Fida Hayati, Septi Nur Maryana, Roni Prasetyo, Dwi Joko Ratnawati, Yuli Ary Ria Suryani, Ria Suwanto, Andri