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All Journal Prosiding Seminar Biologi Berkala Kedokteran Hayyan Journal Illea
Kaswi, Nurwilda
Muhammadiyah Health Polytechnic
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Pengaruh jenis susu terhadap spesies bakteri asam laktat pada dangke asal Kabupaten Enrekang Nurwilda Kaswi; Mochammad Hatta; Rizalinda Sjahril
Prosiding Seminar Biologi Vol 6 No 1 (2020): PROSIDING SEMINAR NASIONAL BIOLOGI DI ERA PANDEMI COVID-19 (OKTOBER 2020)
Publisher : Jurusan Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/psb.v6i1.15884

Abstract

Dangke merupakan makanan khas masyarakat Kabupaten Enrekang, Sulawesi Selatan, Indonesia yang terbuat dari susu berbahan dasar susu sapi atau susu kerbau. Susu merupakan salah satu produk makanan yang secara alami mengandung bakteri asam laktat (BAL). BAL yang berasal dari susu bermanfaat sebagai probiotik dan umumnya Genus Lactobacilli dan Bifidobacteria yang berperan penting dalam menjaga keseimbangan mikroflora usus dan menstimulasi sistem kekebalan tubuh inangnya. Spesies BAL yang paling sering teridentifikasi pada dangke asal Kabupaten Enrekang adalah Lactobacillus acidophilus dan Lactobacillus plantarum. Penelitian ini bertujuan untuk mengetahui ada tidaknya pengaruh jenis bahan dasar susu terhadap spesies BAL pada dangke asal Kabupaten Enrekang. Pada penelitian ini ditemukan sebanyak 9 isolat teridentifikasi bakteri Lactobacillus acidophilus dan 5 isolat teridentifikasi bakteri Lactobacillus plantarum yang terdapat pada sampel dangke susu sapi dan dangke susu kerbau, sehingga disimpulkan bahwa BAL spesies Lactobacillus acidophilus dan Lactobacillus plantarum tidak dipengaruhi oleh perbedaan jenis susu yang merupakan bahan dasar dangke asal Kabupaten Enrekang.
The Use of Multiplex PCR Reactions to Identification of Lactic Acid Bacteria of Dangke Nurwilda Kaswi; Mochammad Hatta; Rizalinda Sjahril
Berkala Kedokteran Vol 19, No 1 (2023)
Publisher : Fakultas Kedokteran Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jbk.v19i1.15723

Abstract

Abstract: Lactic acid bacteria can be isolated from various types of milk including dairy products. Dangke is an Indonesian traditional cheese made by Enrekang community, South Sulawesi. This study aims to identify the lactic acid bacteria by multiplex PCR method which are isolated from dangke obtained from Enrekang Regency. This  type  of  research  used  descriptive research  with  laboratory  observation  methods was performed on 30 cups of dangke are obtained from the markets in 5 sub-districts producing dangke in Enrekang Regency: they are the sub-districts of Cendana, Enrekang, Anggeraja, Alla, and Baraka. The results showed that Lactobacillus acidophilus was the most identified (33.33%) compared to Lactobacillus plantarum (16.67%). Based on the results of the research that has been carried out, it can be concluded that the lactic acid bacteria have been identified in dangke samples obtained from several sub-districts producing dangke in Enrekang Regency are Lactobacillus acidophilus and Lactobacillus plantarum species. Thus, we suggest that this laboratory technique is used to confirm the various species of lactic acid bacteria so it can prove more rapid and exactly that dangke is the rich content of lactic acid bacteria as probiotic candidate.
Comparison of Eosin, Physiological Saline and Lugol Solutions by Using A Native Method for Detection of All Soil-Transmitted Helminths (STH) Kaswi, Nurwilda
Hayyan Journal Vol. 2 No. 1 (2025): February
Publisher : Education and Talent Development Center of Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51574/hayyan.v2i1.2662

Abstract

Stunting can be caused by worm disease because it will interfere with the absorption of nutritional intake, especially carbohydrates and protein, in children. World Health Organization (WHO) data in 2021 reported 24% of the world’s population was infected by Soil Transmitted Helminths (STH). Worms belonging to STH are Ascaris lumbricoides, Trichuris trichiura and hookworms (Ancylostoma duodenale and Necator americanus). The diagnosis of intestinal worms is confirmed by microscopic examination of the feces supported by staining. This study aimed to explore and identify eosin, lugol and physiological saline and evaluate their chemical content as staining solutions to prepare stool samples. A total of 5 stool samples divided into 15 microscopic glass slide were successfully obtained from children around Politeknik Muhammadiyah Makassar. Ascaris lumbricoides was most prevalent helminth found in observations by all the three methods (eosin, lugol and physiological saline wet mount preparation). It was also observed that the young (1-10 years) were more infected because we took samples from childrens around the campus Politeknik Muhammadiyah Makassar. However all the three methods, eosin was found to be effective in coloring the egg stage of the STH parasites. Eosin for wet mount smear in examining the stool samples for detection of intestinal helminths may be routinely used which is simple, clarifying observations under the microscope and increasing the contrast between stained worm eggs and the background because these dye is an acidic compound that is able to fluoresce because it contains bromine and fluorescein so that it can color the cytoplasm and provide a contrast effect so that the boundaries between cells can be observed clearly.
PERBEDAAN IDENTIFIKASI TELUR SOIL TRANSMITTED HELMINTH (STH) PADA SAMPEL FESES ANTARA METODE SEDIMENTASI DENGAN SENTRIFUGASI DAN SEDIMENTASI TANPA SENTRIFUGASI Wahyuni, Sri; Kaswi, Nurwilda; Al Adawiyah, Putri Rabiah; Indra Permata AS
Illea : Journal of Health Sciences, Public Health and Medicine Vol.1, No.2 (2025): Juni 2025
Publisher : Education and Talent Development Center Indonesia (ETDC Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Infeksi cacing adalah infeksi yang dapat ditularkan melalui berbagai cara, tergantung pada jenis cacingnya. Jenis cacing ini tersebar luar di seluruh dunia terutama pada daerah iklim tropis dan sub tropis. Penggunaan metode pemeriksaan tinja yang memiliki sensitivitas dan spesifitas tinggi terhadap Soil Transmitted-Helminth sangat penting untuk deteksi dini infeksi tersebut. Metode sedimentasi yang menggunakan prinsip perbedaan berat jenis merupakan alternatif bagi metode natif. Tujuan penelitian ini adalah untuk mengetahui perbedaan signifikan pemeriksaan feses antara metode sedimentasi dengan menggunakan sentrifugasi dan metode sedimentasi tanpa sentrifugasi. Sampel penelitian ini adalah masyarakat di wilayah Dr. Ratulangi, Desa Mario, Kec. Mariso, Kota Makassar. Hasil penelitian menunjukkan bahwa metode sedimentasi dengan menggunakan sentrifugasi tidak lebih besar memprediksi nilai positif daripada metode sedimentasi tanpa menggunakan sentrifugasi (p>0,05). Metode sedimentasi dengan sentrifugasi adalah metode yang paling baik digunakan sebagai alternatif metode natif dalam hal mendeteksi penyakit Soil-Transmitted Helminth.
Co-Authors Al Adawiyah, Putri Rabiah Mochammad Hatta Mochammad Hatta Permata, Indra Rizalinda Sjahril Rizalinda Sjahril Sri Wahyuni