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Damiyati, Siska Yunita
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Engineering Health Professions Medicine & Pharmacology

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Uji Aktivitas Antiparamphitomiasis Infus Bunga Widuri (Colotropis gigantea) Terhadap Paramphistomum spp. Secara In Vitro Solehah, Kurnia; Damiyati, Siska Yunita; Pratama, Iman Surya; Tresnani, Galuh
Jurnal Kedokteran Vol 9 No 4 (2020): Jurnal Kedokteran volume 9 no 4 2020
Publisher : Faculty of Medicine Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jku.v9i4.454

Abstract

Latar Belakang: Prevalensi paramphistomiasis di provinsi Nusa Tenggara Barat tahun 2014 mencapai 20,7%. Tujuan penelitian untuk membandingkan aktivitas infus bunga Widuri berbagai konsentrasi terhadap Paramphistomum spp. secara in vitro terhadap Albendazol 10% b/v. Metode: Paramphistomum dewasa dikelompokkan ke dalam 5 kelompok : kontrol negatif (larutan NaCl 0,9% b/v), kontrol positif (Albendazol 10% b/v), dan infus bunga Widuri (5, 20 dan 40% b/v). Tiap kelompok diinkubasi pada suhu 37oC, kemudian diamati waktu kematian setiap 15 menit selama 5 jam. Persentase mortalitas ditentukan dan dianalisis secara statistik dengan parametrik dan non-parametrik menggunakan perangkat lunak SPSS v.16; Hasil: Rerata waktu kematian untuk tiap kelompok berturut-turut 270; 23,33 ± 5.77; 16,67 ± 11.55; 103,33 ± 5.77; dan 146,67 ± 5.77 menit. Kesimpulan: Infus bunga Widuri 5% b/v efektif sebagai antiparamphistomiasis ditinjau dari rerata waktu mortalitas yang berbeda bermakna (p<0,05) lebih rendah dibandingkan Albendazol 10% b/v.
In vitro anthelmintic activity of pineapple peel juice (Ananas comosus (L.) Merr.) against Paramphistomum sp. Damiyati, Siska Yunita; Pratama, Iman Surya; Tresnani, Galuh
Communications in Science and Technology Vol 6 No 1 (2021)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21924/cst.6.1.2021.394

Abstract

Pineapple peel (Ananas comosus (L.) Merr.) has a potential natural-based anthelmintic agent. This study aimed to determine the effective concentration of pineapple peel juice against Paramphistomum sp. in vitro. Adults Paramphistomum sp. were divided into 5 test groups, the control group contained Albendazole 10% w/v, the nontreatment group contained NaCl 0.9% w/v and the treatment group contained pineapple peel juice with concentration of 12.5, 20, and 25% w/v. The number of live flukes and the motility scores were recorded every 15 minutes for 5 hours of incubation. The Survival Index (SI) and Relative Motility (RM) of flukes were calculated and statically analyzed using SPSS version 23 software. SI values of flukes in the 12.5, 20, and 25% w/v concentration of treatment group were 46.3, 50.0, and 38.9% respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1, 42.0, and 34.1 respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1359, 42.0249, and 34.1174 respectively. This research showed that, pineapple peel juice 25% w/v was effective against Paramphistomum sp. with survival index and relative motility values comparable to Albendazole 10% w/v (p <0.05).
Co-Authors Galuh Tresnani Pratama, Iman Surya Pratama, Iman Surya Solehah, Kurnia