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The Use of Fish Flour and Palm Sugar Medium to Grow Mataram Indigenous Isolate of Entomopathogenic Bacillus thuringiensis for Controlling Aedes agypti Larvae Suryadi, Bambang Fajar; Hidayat, Hilal Aisyah; Tresnani, Galuh
Journal of Tropical Life Science Vol 9, No 2 (2019)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (154.446 KB) | DOI: 10.11594/jtls.09.02.04

Abstract

To grow Mataram indigenous local isolate of entomopathonic Bacillus thuringiensis using 4 combinations of fish flour and palm sugar natural liquid medium. The culture was then tested against 3rd instar Aedes aegypti larvae to gain lethal concentration (LC) value within 72-hour test. Four combinations of fish flour and palm sugar liquid medium (in 1:1; 1:3; 1:5 and 1:7 weight ratio) were made to grow B. thuringiensis isolate Bt-TP2B. Cell, endospore and protein crystal were recorded every 24 hours within 72-hour incubation. Seventy-two-hour-old B. thuringiensis cultures from those media were serially diluted, then tested against 3rd instar B. thuringiensis larvae. Larval death was recorded every 24 hours within 72-hour observation to obtain lethal concentration (LC) values. Results from all medium were compared to those of NYSM standar medium. B. thuringiensis isolate BT-TP2B could grow in fish flour and palm sugar natural medium. The highest toxicity (the lowest LC) was seen on B. thuringiensis grown on fish flour and palm sugar natural medium in 1:1 weight ratio. The LC50 values were 1.98x103 cell/mL (in 24-hour observation) and 1.49x103 cell/mL (in 48-hour and 72-hour observation), while LC90 values were 1.35x103 cell/mL in 24-hour observation and 1.58x103 cell/mL in 48 and 72-hour observations). These values were lower (more toxic) compared to those of NYSM standard medium. Fish flour and palm sugar natural medium could be used to grow entomopathogenic B. thuringiensis. Moreover, this natural medium could increase toxicity of B. thuringiensis against B. thuringiensis3rd instar larvae. 
Uji Aktivitas Antiparamphitomiasis Infus Bunga Widuri (Colotropis gigantea) Terhadap Paramphistomum spp. Secara In Vitro Solehah, Kurnia; Damiyati, Siska Yunita; Pratama, Iman Surya; Tresnani, Galuh
Jurnal Kedokteran Vol 9 No 4 (2020): Jurnal Kedokteran volume 9 no 4 2020
Publisher : Faculty of Medicine Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jku.v9i4.454

Abstract

Latar Belakang: Prevalensi paramphistomiasis di provinsi Nusa Tenggara Barat tahun 2014 mencapai 20,7%. Tujuan penelitian untuk membandingkan aktivitas infus bunga Widuri berbagai konsentrasi terhadap Paramphistomum spp. secara in vitro terhadap Albendazol 10% b/v. Metode: Paramphistomum dewasa dikelompokkan ke dalam 5 kelompok : kontrol negatif (larutan NaCl 0,9% b/v), kontrol positif (Albendazol 10% b/v), dan infus bunga Widuri (5, 20 dan 40% b/v). Tiap kelompok diinkubasi pada suhu 37oC, kemudian diamati waktu kematian setiap 15 menit selama 5 jam. Persentase mortalitas ditentukan dan dianalisis secara statistik dengan parametrik dan non-parametrik menggunakan perangkat lunak SPSS v.16; Hasil: Rerata waktu kematian untuk tiap kelompok berturut-turut 270; 23,33 ± 5.77; 16,67 ± 11.55; 103,33 ± 5.77; dan 146,67 ± 5.77 menit. Kesimpulan: Infus bunga Widuri 5% b/v efektif sebagai antiparamphistomiasis ditinjau dari rerata waktu mortalitas yang berbeda bermakna (p<0,05) lebih rendah dibandingkan Albendazol 10% b/v.
In vitro anthelmintic activity of pineapple peel juice (Ananas comosus (L.) Merr.) against Paramphistomum sp. Damiyati, Siska Yunita; Pratama, Iman Surya; Tresnani, Galuh
Communications in Science and Technology Vol 6 No 1 (2021)
Publisher : Komunitas Ilmuwan dan Profesional Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21924/cst.6.1.2021.394

Abstract

Pineapple peel (Ananas comosus (L.) Merr.) has a potential natural-based anthelmintic agent. This study aimed to determine the effective concentration of pineapple peel juice against Paramphistomum sp. in vitro. Adults Paramphistomum sp. were divided into 5 test groups, the control group contained Albendazole 10% w/v, the nontreatment group contained NaCl 0.9% w/v and the treatment group contained pineapple peel juice with concentration of 12.5, 20, and 25% w/v. The number of live flukes and the motility scores were recorded every 15 minutes for 5 hours of incubation. The Survival Index (SI) and Relative Motility (RM) of flukes were calculated and statically analyzed using SPSS version 23 software. SI values of flukes in the 12.5, 20, and 25% w/v concentration of treatment group were 46.3, 50.0, and 38.9% respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1, 42.0, and 34.1 respectively. RM values of flukes in the 12.5, 20 and 25% w/v concentration of treatment groups were 39.1359, 42.0249, and 34.1174 respectively. This research showed that, pineapple peel juice 25% w/v was effective against Paramphistomum sp. with survival index and relative motility values comparable to Albendazole 10% w/v (p <0.05).
NEMATODE PARASITES OF RATS FROM KALIMANTAN Saputri, Ferza Ayu; Dewi, Kartika; Tresnani, Galuh; Apriyanti, Yuni; Supriatna, Nanang; Fitriana, Yuli S.; Zamroni, Yuliadi
TREUBIA Vol 51, No 2 (2024)
Publisher : National Research and Innovation Agency

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/treubia.v51i2.4845

Abstract

Rats can serve as a reservoir of diseases because the parasites within rats can be transmitted to humans and other animals. One of the parasites comes from the phylum Nematoda. This study aimed to identify the nematode species found in rats, determine their prevalence and intensity in Kalimantan Island. This study used 20 carcasses of rats from Kalimantan Island stored in the wet collection of MZB (Museum Zoologicum Bogoriense) BRIN Cibinong-Bogor. Identification was carried out by observing and measuring the nematodes found using a light microscope and Scanning Electron Microscope (SEM). The nematode species were then calculated for prevalence and intensity in each host. In this study, four species of nematodes, including Subulura (Murisubulura) andersoni (10% and 11-35), Subuluridae gen. sp. (80% and 1-53), Mastophorus sp. (25% and 1), and Physocephalus sp. (25% and 5), were found. Kalimantan Island is a new record area for S (M.) andersoni, Mastophorus sp. and Physocephalus sp. This needs further research to identify Subuluridae gen.sp. and its distribution area in Indonesia.
Brief Report on The Population Survey Long-tailed Macaque (Macaca fascicularis [Raffles,1821]) in West Nusa Tenggara, Indonesia Hadi, Islamul; Wiantoro, Sigit; Inayah, Nurul; Maharadatunkamsi, Maharadatunkamsi; Atikah, Tika Dewi; Zamroni, Yuliadi; Supardiono, Supardiono; Alfian, Muhammad Rijal; Sadewo, Imran; Mubarokah, Nurlaila; Kurniawan, Budhy; Tresnani, Galuh
JURNAL SAINS TEKNOLOGI & LINGKUNGAN Vol. 10 No. 3 (2024): JURNAL SAINS TEKNOLOGI & LINGKUNGAN
Publisher : LPPM Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jstl.v10i3.701

Abstract

Survey on the population and distribution of long-tailed macaque (Macaca fascicularis [Raffles, 1821]) in the administrative areas of West Nusa Tenggara Province have been conducted during June-July 2024 to examine the distribution and population status on the macaque.  The on-road reconnaissance survey, interview and literature studies have applied to collect data. We recorded 6146 individuals from 307 groups of long-tailed macaques in eight regencies. Of the 6146 individuals of long-tailed macaques, 1532 individuals are recorded living in conservation area, while 4614 individuals live in outside conservation area, respectively.  The number of individuals and troops of the monkey showed us the minimum number that can be recorded. Further study needs to be conducted in addressed to dig more information on the existence of long-tailed macaque in West Nusa Tenggara. Furthermore, population information and distribution of long-tailed macaques could be the basic knowledge on the population management efforts in the future.
Population Density and Habitat Preferences of the Rinjani Scops Owl (Otus jolandae) in the Mandalika Special Economic Zone, Lombok, Indonesia: Population density and habitat preference of Otus jolandae Permatasari, Baiq Dewi; Suana, I Wayan; Hadiprayitno, Gito; Tresnani, Galuh
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/e0jyvz11

Abstract

The Rinjani Scops Owl (Otus jolandae) is an endemic species of Lombok, closely associated with Mount Rinjani National Park. In 2023, its range was newly recorded in the Mandalika Special Economic Zone (SEZ), extending its known distribution. Understanding the population density and habitat preferences of this bird is crucial for effective management and conservation. From April to June 2024, a study was conducted in Mandalika SEZ, covering an area of 1,175 hectares. The point count method was used to record individuals based on sightings and calls. Vegetation analysis was performed at observation sites to identify preferred tree species and calculate habitat preferences using the selection index. Twenty-three individuals of O. jolandae were observed across seven sites, with a population density of 0.02 individuals per hectare—lower than densities recorded in Mount Rinjani National Park—which reflects the significant ecological pressures from development activities. The habitat includes 21 tree species, but the owl actively uses only 14 for activities such as perching, roosting, and nesting. Samanea saman had the highest preference index (3.424), making it the most favored tree, followed by Gmelina arborea and Nauclea orientalis. The species relies heavily on large-diameter trees and the middle canopy layer, reflecting its need for stable and protective habitats. This study provides essential data for future conservation programs and highlights the Mandalika SEZ’s potential as a birdwatching ecotourism destination featuring this unique endemic species
Analisis aktivitas antelmintik ekstrak etanol daun Mimosa pudica L. terhadap Ascaridia galli Tresnani, Galuh; Martini, Rima; Pratama, Iman Surya
Samota Journal of Biological Sciences Vol 1 No 1 (2022): Samota Journal of Biological Sciences
Publisher : University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/sjbios.v1i1.628

Abstract

Mimosa pudica leaves contain of metabolites such as tannin, flavonoid and alkaloid which have the anthelmintic effects. This effect would be expected to minimalize the infection of parasitic worm Ascaridia galli in chicken. The research aim was to determine the effectivity of ethanol extract from mimosa leaves to A. galli worm in vitro. Mimosa leaves were extracted using maceration process. Worm samples were grouped randomly, consist of the positive control (Piperazine 1%), negative control (CMC Na 0.5%) and the treatment groups (10%, 20% and 50% of extract concentration). Mortality and paralysis data from the worm samples were collected and analyzed using Kruskal Wallis and Mann Whitney Test. The phytochemical analysis showed that mimosa leaves extract contains all the secondary metabolite which can cause paralysis and mortality of A. galli. The 50% concentration of mimosa leaves extract can cause paralysis in 90 minutes equal with the positive control. The mortality time achieved by positive control, 50%, 20% and 10% of ethanol extract of mimosa leaves sequentially as follows 120, 270, 645 and 780 minutes. In conclusion, 50% mimosa leaves extract concentration have the best effect of anthelmintic, the paralysis effect equal with Piperazine but the mortality time is still slower than Piperazine.
Detection of Bacterial Contaminants and Identification of Salmonella Sp. in Cilok Snack in Selaparang District, Mataram Putri, Vita Septiana Sulaiman; Faturrahman, Faturrahman; Sarkono, Sarkono; Tresnani, Galuh; Suryadi, Bambang Fajar
Samota Journal of Biological Sciences Vol 2 No 1 (2023): Samota Journal of Biological Sciences
Publisher : University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/sjbios.v2i1.2885

Abstract

Cilok is a boiled snack made from starch with or without a mixture of minced meat, has a savory and chewy taste. Cilok is one of the favorite snacks in Indonesia. Most cilok sellers sell their cilok right on the side of the main road for hours. This allows cilok that are sold to be contaminated with various bacteria. This study aims to calculate the Total Plate Count (TPC) of bacteria and to identify Salmonella sp. in cilok and its sauce in Selaparang District, Mataram City. This study used quantitative method in the form of calculating the TPC of the bacteria and qualitative method in the form of sample isolation on selective media, sugar, and biochemical tests. The results of this study showed that the TPC of the bacteria from the cilok sellers samples number 1 to 8 were 1,2x102, 1x101, 1x101, 2x101, 1,2x102, 1,5x102, 3,9x101, and 3,5x102 cfu/g respectively. Meanwhile, the TPC of the bacteria from sellers' sauce samples number 1 to 8 were 2x102, 1,8x103, 1x101, 1x101, 1,5x101, 7,9x103, 1,7x105 and 1,7x103 cfu/mL, and there was no contamination of Salmonella sp. on cilok nor its sauce samples. All the cilok samples that has been examined had TPC bacterial value that did not exceed the standards set by SNI 7388-2009, so they were still safe for consumption. Meanwhile, one out of eight sauce samples had a bacterial TPC value that exceeded the standard, making it not safe for consumption.
HABITAT PERINDUKAN Ae. albopictus DI PANTAI PENGHULU AGUNG DAN SEKIP, AMPENAN, NUSA TENGGARA BARAT Pita, Baiq Nuraini Ayu; Tresnani, Galuh; Suryadi, Bambang Fajar; Zamroni, Yuliadi; Kusuma, AA Ngurah Nara
Samota Journal of Biological Sciences Vol 3 No 1 (2024): Samota Journal of Biological Sciences
Publisher : University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/sjbios.v3i1.4003

Abstract

Ampenan is one of a coastal area with a dense population in Mataram City. There are lots of people activity in Penghulu Agung and Sekip beach since they are the recreational area in Ampenan District, and therefore diseases transmission through mosquito bites becomes easily. The research aims are to determine the abundance and habitat characteristics of Aedes albopictus as a vector of dengue fever in Penghulu Agung and Sekip beach. This esearch is a descripftive explorative research using random sampling and explorative methods. According to research results it can be conclude that the relative abundance value of Aedes albopictus from both location is 53.80% (high). The breeding habitat mostly in the form of bucket and boat with a dense of vegetation or canopi. environmental factors in the research location such as water temperature range between 29.8 - 30.5 Celcius, air temperatur between 25.5 - 29.9 Celcius, humidity between 52 - 76%, water acidity 6 - 8, salinity 0%, and light intensity 105 - 30,430 lux.