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GAMBARAN STATUS KEBERSIHAN GIGI DAN MULUT SERTA KEBUTUHAN PERAWATAN PERIODONTAL PADA REMAJA YANG MENGKONSUMSI ALKOHOL Hervina, dr.; Dwis Syahriel; IGA Dewi Haryani
Jurnal Bakti Saraswati (JBS): Media Publikasi Penelitian dan Penerapan Ipteks Vol 9 No 2 (2020): Jurnal Bakti Saraswati (JBS) : Media Publikasi Penelitian dan Penerapan Ipteks
Publisher : Lembaga Penelitian dan Pemberdayaan (Institute for Research and Community Empowerment) Universitas Mahasaraswati Denpasar Bali

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Konsusmsi alkohol secara berlebihan diketahui memiliki efek yang buruk bagi kesehatan khususnya jaringan periodontal. WHO menyatakan bahwa sebesar 43% atau 2,3 milyar dari populasi penduduk di dunia mulai umur 15 tahun ke atas mengkonsumsi alkohol. Penelitian deskriptif ini bertujuan untuk mengetahui gambaran status kebersihan gigi dan mulut serta kebutuhan perawatan periodontal pada remaja yang mengkonsumsi alkohol. Jenis penelitian ini adalah penelitian deskriptif dengan pendekatan cross sectional dilakukan pada 54 remaja SMP usia 13-15 tahun yang mengkonsumsi alkohol di Lingkungan Banjar Purnama Asri Desa Peguyangan Kangin Kecamatan Denpasar Utara. Pengukuran status kebersihan mulut menggunakan indeks OHI-s dan kebutuhan perawatan periodontal menggunakan Community Periodontal Index of Treatment Needs (CPITN). Sebanyak 29,63% memiliki status OHI-s baik, 22,22% sedang dan 48,15% buruk. Berdasarkan skor CPITN maka sebanyak 20,37% memerlukan kindakan edukasi instruksi kesehatan mulut dan 51,85% memerlukan tidakan edukasi instruksi kesehatan mulut serta perawatan pembersihan karang gigi (skaling), sedangkan 27,78% dalam kondisi sehat tanpa membutuhkan perawatan periodontal. Simpulan: kondisi status kebersihan mulut remaja peminum alkohol paling banyak status buruk dan Sebagian besar memerlukan edukasi instruksi kesehatan mulut serta pembersihan karang gigi (skaling).

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO Syahrul, Dwis; Syahriel, Dwis
Interdental: Jurnal Kedokteran Gigi Vol 17 No 2 (2021): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Faculty of Dentistry, Mahasaraswati Denpasar University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v17i2.2870

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro.

GAMBARAN STATUS KEBERSIHAN GIGI DAN MULUT SERTA KEBUTUHAN PERAWATAN PERIODONTAL PADA REMAJA YANG MENGKONSUMSI ALKOHOL Hervina Hervina; Dwis Syahriel; IGA Dewi Haryani
Jurnal Bakti Saraswati (JBS): Media Publikasi Penelitian dan Penerapan Ipteks Vol. 9 No. 2 (2020): Jurnal Bakti Saraswati (JBS) : Media Publikasi Penelitian dan Penerapan Ipteks
Publisher : Lembaga Penelitian dan Pengabdian Masyarakat (Institute for Research and Community Empowerment) Universitas Mahasaraswati Denpasar Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar

Konsusmsi alkohol secara berlebihan diketahui memiliki efek yang buruk bagi kesehatan khususnya jaringan periodontal. WHO menyatakan bahwa sebesar 43% atau 2,3 milyar dari populasi penduduk di dunia mulai umur 15 tahun ke atas mengkonsumsi alkohol. Penelitian deskriptif ini bertujuan untuk mengetahui gambaran status kebersihan gigi dan mulut serta kebutuhan perawatan periodontal pada remaja yang mengkonsumsi alkohol. Jenis penelitian ini adalah penelitian deskriptif dengan pendekatan cross sectional dilakukan pada 54 remaja SMP usia 13-15 tahun yang mengkonsumsi alkohol di Lingkungan Banjar Purnama Asri Desa Peguyangan Kangin Kecamatan Denpasar Utara. Pengukuran status kebersihan mulut menggunakan indeks OHI-s dan kebutuhan perawatan periodontal menggunakan Community Periodontal Index of Treatment Needs (CPITN). Sebanyak 29,63% memiliki status OHI-s baik, 22,22% sedang dan 48,15% buruk. Berdasarkan skor CPITN maka sebanyak 20,37% memerlukan kindakan edukasi instruksi kesehatan mulut dan 51,85% memerlukan tidakan edukasi instruksi kesehatan mulut serta perawatan pembersihan karang gigi (skaling), sedangkan 27,78% dalam kondisi sehat tanpa membutuhkan perawatan periodontal. Simpulan: kondisi status kebersihan mulut remaja peminum alkohol paling banyak status buruk dan Sebagian besar memerlukan edukasi instruksi kesehatan mulut serta pembersihan karang gigi (skaling).

Infiltrasi Neutrofil Pada Penyembuhan Luka Insisi Gingiva Tikus Wistar Setelah Pemberian Vitamin D Hervina Hervina; Dwis Syahriel; I Gusti Ngurah Gede Swarga Prawira
JBN (Jurnal Bedah Nasional) Vol 5 No 2 (2021): JBN (Jurnal Bedah Nasional)
Publisher : Program Studi Ilmu Bedah, Fakultas Kedokteran Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBN.2021.v05.i02.p02

Latar belakang: Prevalensi periodontitis di Indonesia sebesar 74,1% lebih tinggi dibandingkan negara-negara lainnya di dunia. Perawatan periodontitis seperti tindakan bedah periodontal meliputi tindakan insisi akan menimbulkan luka pada gingiva. Penyembuhan luka pada rongga mulut harus diperhatikan agar tidak terjadi infeksi. Vitamin D memiliki efek antiinflamasi sebagai imunomodulator dalam mekanisme penyembuhan luka. Tujuan penelitian ini untuk membuktikan efek antiinflamasi vitamin D terhadap infiltrasi neutrofil pada luka insisi gingiva tikus wistar. Metode: Rancangan post test only control group design pada 24 sampel yang dilakukan insisi pada gingiva labial insisivus rahang bawah, dibagi menjadi 6 kelompok: kontrol yaitu tanpa pemberian perawatan observasi hari ke-1 (K1) dan hari ke-3 (K2); perlakuan 1 (pemberian vitamin D 1000 IU per-oral/perhari) observasi hari ke-1 (P1) dan hari ke-3 (P2), perlakuan 2 (pemberian vitamin D 1000 IU per-oral/hari dan Povidone iodin 1% secara topikal ) observasi hari ke-1 (P3) dan hari ke-3 (P4). Pembuatan preparat dari jaringan gingiva area insisi dengan pewarnaan HE dan perhitungan jumlah sel neutrofil menggunakan mikroskop pembesaran 400 kali. Analisis data dengan Uji One Way Anova kemudian dilanjutkan dengan uji Least Significant Difference. Hasil: Terdapat perbedaan rerata jumlah neutrofil pada keenam kelompok nilai F = 12,03 dan p = 0,000. Perbedaan terdapat pada P1-K1 (p=0,000), P1-P3 (p=0,021), P1-P2 (p=0,047), P3-K1 (p=0,01), P2-K2 (p=0,002), P2-K4 (p=0,047). Simpulan: Infiltrasi neutrofil pada penyembuhan luka insisi gingiva tikus lebih tinggi pada pemberian vitamin D pada hari ke-1 dibanding kontrol, dan infiltrasi neutrofil hari ke-3 lebih rendah dibanding hari ke-1 setelah pemberian vitamin D.

OPTIMAL CONCENTRATION OF BASIL LEAF EXTRACT (Ocimum basilicum L.) IN INHIBITING THE GROWTH OF Streptococcus mutans IN VITRO : Konsentrasi Optimal Dari Ekstrak Daun Kemangi (Ocimum Basilicum L.) Dalam Menghambat Pertumbuhan Bakteri Streptococcus Mutans In Vitro Dwis Syahrul; Dwis Syahriel
Interdental Jurnal Kedokteran Gigi (IJKG) Vol. 17 No. 2 (2021): Interdental Jurnal Kedokteran Gigi (IJKG)
Publisher : Fakultas Kedokteran Gigi, Universitas Mahasaraswati Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46862/interdental.v17i2.2870

Introduction: One of flora in the oral cavity is Streptococcus mutans as a cause of dental caries. Various ways can be done to suppress its growth, one of them by using mouthwash which is used contains a lot of chemicals, so an alternative is needed by using herbal ingredients, including basil (Ocimum basilicum L.) leaves that contain essential oils, methyl eugenol, phenols, and flavonoids which are able to work as antibacterial. The purpose of this study was to determine the inhibition and optimal concentration of basil leaf extract on the growth of Streptococcus mutans. Materials and method: The method used agar Kirby Bauer method with seven treatments of leaf extract with concentrations of 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, and methanol solution as control group. The culture medium used was Muller Hinton Blood Agar. Results and discussions: The Kruskall Wallis test showed a significant difference between treatment groups. Mann Whitney U-Test test, found that the control group, basil leaf extract concentrations of 1.5%, 2%, 2.5% and 3% were tested with concentrations of 3.5% and 4% had a significant difference. The test between groups of 3.5% and 4% basil leaf extract did not show a significant difference. Conclusion: this study was that in vitro basil leaf extract with a concentration of 3.5% and 4% had inhibitory power on the growth of Streptococcus mutans and basil leaf extract with a concentration of 3.5% optimally inhibited the growth of Streptococcus mutans in vitro.

Vitamin D inhibits TNF-α serum level in wistar rats stimulated with Porphyromonas gingivalis Hervina, Hervina; Syahriel, Dwis; Wedagama, Dewa Made; Nurlitasari, Dewi Farida; Nasutianto, Haris; Haryani, I Gusti Ayu Dewi
Majalah Kedokteran Gigi Indonesia Vol 9, No 3 (2023): December
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.81563

Periodontitis is a chronic inflammation of the periodontal tissues initiated by increased proinflammatory cytokines such as TNF-α due to the induction of Porphyromonas gingivalis (Pg). This study aimed to analyze the effect of vitamin D (cholecalciferol) on TNF-α serum level in Wistar rats stimulated with Pg to induce periodontitis. Twenty-seven male Wistar rats (n = 27) were divided into three equal groups. Group I was healthy Wistar rats that received 2000 IU vitamin D once a day. Group II was Wistar rats stimulated with Pg and received 2000 IU vitamin D, while group III was Wistar rats stimulated with Pg but did not receive vitamin D. Blood was collected through the orbital sinus and centrifuged to get the serum. TNF-α serum levels were assessed using Elisa method on the 7th, 14th, and 28th. The data were normally distributed and homogeneous. The mean TNF-α data was analyzed for differences between groups using the one-way ANOVA and LSD post hoc test. Significant differences were seen in group II (418.49 ± 161.08 ng/mL) and group III (172.16 ± 104.18 ng/mL) on the 28th day (p = 0.001). The findings suggest that vitamin D inhibits the TNF-α serum level in Wistar rats stimulated with Pg on the 28th day.

Antibacterial Activity of Bidara Arab Leaves (Ziziphus spina-christi L) Extract Against Gram-negative Anaerobic Subgingival Bacteria Syahriel, Dwis; Hervina, Hervina; Sardi, Ni Wayan Arni
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.241-250

Background: Gram-negative anaerobic subgingival bacteria play an important role in the initiation and progression of periodontitis. The secondary metabolites contained in Bidara Arab leaves (Ziziphus spina-christi L) have potential to be antibacterial and inhibited the growth of pathogenic bacterial. The purposed of this study to evaluated the antibacterial activity of Bidara Leaves extract against gram-negative anaerobic subgingival bacteria. Method: Experimental laboratories using Mueller hinton agar to determine the antibacterial activity of Bidara leaves extract 40% and 60% against Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum, with a comparison of the negative control group ethanol 96% and tetracycline as positive control. The production of extracts through maseration techniques and the subsequent phytochemical testing through tube reaction test. Result: The results of phytochemical analysis identified the presence of flavonoids, tannins, saponins, steroids. The inhibition test showed that the Bidara leaves extract exhibited strong inhibition and a concentration of 60% was more effective than 40% against Gram-negative anaerobic subgingival bacteria including Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum. Conclusion: The Bidara Arab leaves (Ziziphus spina-christi L) extract at concentration 60% was more effective than 40% against gram-negative anaerobic bacteria

Antibacterial Activity of Bidara Arab Leaves (Ziziphus spina-christi L) Extract Against Gram-negative Anaerobic Subgingival Bacteria Syahriel, Dwis; Hervina, Hervina; Sardi, Ni Wayan Arni
Odonto : Dental Journal Vol 11, No 2 (2024): December 2024
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.11.2.241-250

Background: Gram-negative anaerobic subgingival bacteria play an important role in the initiation and progression of periodontitis. The secondary metabolites contained in Bidara Arab leaves (Ziziphus spina-christi L) have potential to be antibacterial and inhibited the growth of pathogenic bacterial. The purposed of this study to evaluated the antibacterial activity of Bidara Leaves extract against gram-negative anaerobic subgingival bacteria. Method: Experimental laboratories using Mueller hinton agar to determine the antibacterial activity of Bidara leaves extract 40% and 60% against Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum, with a comparison of the negative control group ethanol 96% and tetracycline as positive control. The production of extracts through maseration techniques and the subsequent phytochemical testing through tube reaction test. Result: The results of phytochemical analysis identified the presence of flavonoids, tannins, saponins, steroids. The inhibition test showed that the Bidara leaves extract exhibited strong inhibition and a concentration of 60% was more effective than 40% against Gram-negative anaerobic subgingival bacteria including Porphyromonas gingivalis, Aggregatibacter ctinomycetemcomitans, and Fusobacterium nucleatum. Conclusion: The Bidara Arab leaves (Ziziphus spina-christi L) extract at concentration 60% was more effective than 40% against gram-negative anaerobic bacteria

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