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Sri Laksemi, Dewa Ayu Agus
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Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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UJI EFEKTIVITAS KOMBINASI EKSTRAK DAUN SIRIH HIJAU “PIPER BETLE L” DAN MADU SEBAGAI ANTIMALARIA PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI Swastika, I Nengah Raka; Sri Laksemi, Dewa Ayu Agus; Damayanti, Putu Ayu Asri; Diarthini, Ni Luh Putu Eka
E-Jurnal Medika Udayana Vol 12 No 8 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i08.P03

Abstract

Malaria is an infectious disease caused by the parasite Plasmodium sp. transmitted by the female Anopheles mosquito vector through its bite. Patients infected with malaria worldwide in the last two decades have increased, mainly due to the emergence of strains of Plasmodium falciparum that have become resistant to antimalarial drugs. This has resulted in the emergence of efforts to find alternative medicinal plants that can overcome this problem. One of the plants that have the potential to be developed is Piper betle L, or commonly known as green betel plant. This study aims to determine the effect of green betel leaf extract (Piper betle L) and honey at doses of 50, 100, and 200 mg/kgBW as antimalarials in Balb/c mice infected with Plasmodium berghei. This study is an in vivo experimental study using the Randomized Post-test Only Controlled Group method design using 24 samples of mice which were divided into four groups, one negative control group and three treatment groups. The average degree of parasitemia in the control group was 45.51%, the treatment group at a dose of 50 mg/kgBW was 35.71%, a dose of 100 mg/kgBW was 30.96% and a dose of 200 mg/kgBW was 31.67%. Based on the results of the analysis using One Way ANOVA, it was found that the mean difference between the control and treatment groups with p value <0.001 was found. The results showed that green betel leaf extract (Piper betle L) could inhibit the growth of Plasmodium berghei by looking at the mean difference between the control group and the treatment group and obtained that the average treatment group of 100 mg/kgBW and 200 mg/kgBW were equally effective at inhibiting parasitemia levels. Keywords : Antimalarial, Piper betle L, Degree of Parasitemia
THE EFFECTIVENESS OF CORN (Zea mays L.) Immersion Water as an ATTRACTAN FOR THE OVITRAP OF THE AEDES AEGYPTI MOSQUITO Dana, I Kadek; Asri Damayanti, Putu Ayu; Eka Diarthini, Ni Luh Putu; Sri Laksemi, Dewa Ayu Agus
E-Jurnal Medika Udayana Vol 12 No 8 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i08.P04

Abstract

ABSTRAK Penggunaan ovitrap dengan modifikasi penambahan atraktan telah dikenal sebagai metode alternatif dalam pengendalian vektor DBD (Demam Berdarah Dengue). Bahan atraktan dapat dibuat dari bahan alami yang mudah dijumpai sehingga ramah lingkungan dan murah. Tanaman jagung cukup mudah diperoleh serta mudah diolah sebagai bahan atraktan. Penelitian ini bertujuan untuk mengetahui perbedaan efektivitas air rendaman kulit jagung (Zea mays L.) dalam berbagai konsentrasi sebagai atraktan nyamuk Aedes aegypti. Penelitian dengan desain eksperimental menggunakan rancangan acak lengkap. Bahan penelitian berupa kulit jagung dengan kondisi kering dan direndam selama tujuh hari menggunakan ember yang tertutup. Sampel penelitian adalah nyamuk Aedes aegypti betina kenyang darah yang dibagi menjadi 5 kelompok yaitu 4 kelompok perlakuan yang masing-masing air rendaman jagung konsentrasi 5%, 10%, 15%, 20% dan satu kelompok tanpa rendaman air jagung . Setiap kelompok terdiri dari 25 ekor nyamuk dan replikasi dilakukan sebanyak 5 kali. Selama tujuh hari pengamatan jumlah telur tertinggi terdapat pada hari kelima dengan jumlah rata-rata telur adalah konsentrasi 5% (100,60±67,09), 10% (30,40±16,80), 15% (2,80±2,94), 20% (2,00±2,44).Least Significant Differences) pada perlakuan akuades-atraktan 5% (p= 0,027), atraktan 5%-10% (p= 0,005), atraktan 5%-15% (p= 0,000), dan atraktan 5%-20% (p= 0,000). Kkonsentrasi atraktan 5% efektif dalam meningkatkan jumlah telur pada ovitrap. Kata kunci : Aedes aegypti, Kulit jagung (Zea mays L.), Atraktan
THE RELATIONSHIP BETWEEN KNOWLEDGE LEVEL AND PERSONAL HYGIENE BEHAVIOR ON THE INCIDENCE OF PEDICULOSIS IN ELEMENTARY SCHOOL CHILDREN IN NUSA PENIDA. Bisma Krisnanda, I Kadek; Sri Laksemi, Dewa Ayu Agus; Asri Damayanti, Putu Ayu; Eka Diarthini, Ni Luh Putu
E-Jurnal Medika Udayana Vol 13 No 8 (2024): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2024.V13.i08.P08

Abstract

ABSTRACT Pediculosis capitis is an infection that occurs on the human scalp due to an infestation of Pediculus humanus capitis. Pediculosis is characterized by itching on the scalp, in school-age children the infestation of Pediculus humanus capitis has a negative impact on children's learning activities and has the potential to interfere with learning achievement. This study aims to determine whether personal hygiene and knowledge have a relationship with the incidence of pediculosis in elementary school children in Nusa Penida. This study uses an analytic observational study with a Cross Sectional study with univariate analysis techniques and bivariate analysis using the Chi-square test, this study used a sample of 100 samples taken from SD Negeri 2 Ped and SD Negeri 4 Ped, data collection was carried out by using a questionnaire regarding personal hygiene behavior and knowledge of pediculosis and the incidence of pediculosis in research respondents. In this study it was found that out of 100 respondents, 44% had poor personal hygiene, 41% had poor knowledge and 65% experienced pediculosis which is a high number because it exceeds 50%, from this study it was found that personal hygiene had a significant relationship with the incidence of pediculosis (p-value 0.002 and RP = 1.580), and knowledge about pediculosis has a relationship with the incidence of pediculosis (p-value 0.002 and RP = 1.578). So it can be seen that personal hygiene and knowledge about pediculosis is one of the risk factors for the incidence of pediculosis in elementary school children in Nusa Penida. Keywords : Personal hygiene, Knowledge, Pediculosis
UJI EFEKTIVITASAN BUAH MOMORDICA CHARANTIA L SEBAGAI IMUNOSTIMULAN DAN ANTIPLASMODIUM TERHADAP INFEKSI PARASIT PLASMODIUM BERGHEI PADA MENCIT Semara, I Nyoman Sudha; Sri Laksemi, Dewa Ayu Agus; Damayanti, Putu Ayu Asri; Eka Diarthini, Ni Luh Putu
E-Jurnal Medika Udayana Vol 12 No 12 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i12.P11

Abstract

ABSTRAK Malaria adalah penyakit infeksi parasit Plasmodium sp. Plasmodium merupakan parasit yang menyerang sel darah merah dan dapat menimbulkan obstruksi pada kapiler organ tubuh yang vital. Kasus resisten plasmodium terhadap obat anti malaria membuat banyak penelitian yang mencoba mencari obat alternatif dari tumbuhan yang mudah diperoleh masyarakat, salah satu tanaman yang memiliki potensi yakni Momordica charantia L atau yang dikenal buah pare. Untuk mengetahui pengaruh ekstrak buah pare (Momordica charantia L) dengan dosis 100 mg/kgBB untuk antiplasmodium dan 100 mg/kgBB untuk immunostimulant terhadap derajat parsitemia pada mencit Balb/c yang diinfeksikan Plasmodium Berghei. Penelitian ini merupakan penelitian eksperimental secara In Vivo dengan menggunakan rancangan metode Randomized Post test Only Controlled Group menggunakan 24 sampel mencit yang dibagi menjadi empat kelompok yakni satu kelompok kontrol negatif antiplasmodium, satu kelompok negatif immunostimulant, satu kelompok perlakuan antiplasmodium dan satu kelompok perlakuan immunostimulant. Didapatkan hasil rerata derajat parasitemia pada kelompok kontrol negatif antiplasmodium 34.9 %, kelompok kontrol negatif immunostimulant 33.9 %, kelompok perlakuan antiplasmodium dosis 100 mg/kgBB sebesar 19.8 % dan kelompok perlakuan immunostimulant dosis 100 mg/kgBB sebesar 17.1 %. Berdasarkan hasil analisis One Way Anova ditemukan perbedaan rerata antara masing-masing kelompok kontrol dan kelompok perlakuan yakni kelompok antiplasmodium dengan nilai p value <0.001 dan kelompok immunostimulant dengan nilai p value <0.002. Hasil penelitian menunjukan ekstrak buah pare (Momordica charantia L) dapat menghambat pertumbuhan parasit Plasmodium Berghei dengan melihat perbedaan rerata masing-masing kelompok kontrol dan kelompok perlakuan, dengan masing-masing dosis kelompok yakni 100 mg/kbBB. Kata kunci: Antimalaria, Momordica charantia L, Plasmodium Berghei, Derajat Parasitemia
UJI EFEKTIVITAS EKSTRAK DAUN KELOR “MORINGA OLEIFERA” SEBAGAI IMUNOSTIMULAN DAN ANTIPLASMODIUM PADA MENCIT YANG DIINFEKSI PLASMODIUM BERGHEI Sujaya, I Made Pranawa Yogananda; Sri Laksemi, Dewa Ayu Agus; Asri Damayanti, Putu Ayu; Eka Diarthini, Ni Luh Putu
E-Jurnal Medika Udayana Vol 12 No 12 (2023): E-Jurnal Medika Udayana
Publisher : Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/MU.2023.V12.i12.P13

Abstract

Malaria merupakan penyakit yang sangat umum dan kompleks terjadi di Indonesia. Penyakit ini merupakan suatu penyakit yang disebabkan oleh parasit yang masuk kedalam genus Plasmodium dan tergolong protozoa obligat intraseluler. Banyaknya kasus resistensi obat antimalaria di Indonesia menyebabkan muncul suatu inovasi untuk menggunakan obat herbal sebagai penggantinya. Daun Kelor (Moringa oleifera) merupakan salah satu solusi dari masalah ini karena kandungan antioksidan di dalamnya. Untuk mengetahui aktivitas daun kelor (Moringa oleifera) sebagai imunostimulan dan antiplasmodium terhadap Plasmodium berghei secara in vivo. Penelitian merupakan penelitian eksperimental secara in vivo dengan 4 kelompok sampel terbagi dengan 2 kelompok kontrol dan 2 kelompok perlakuan dengan total mencit 24 ekor. Didapatkan hasil rerata derajat parasitemia pada kelompok kontrol antiplasmodium sebesar 35,7% dan kelompok kontrol imunostimulan sebesar 36,4%, sedangkan pada kelompok perlakuan antiplasmodium sebesar 17,1% dan pada kelompok perlakuan imunostimulan sebesar 17,6%. Berdasarkan hasil analisis dengan One Way ANOVA ditemukan perbedaan rerata antara kelompok kontrol dan perlakuan dengan nilai p value <0,000. Penelitian mendapatkan hasil bahwa ekstrak daun kelor (Moringa oleifera) dapat menghambat pertumbuhan dari Plasmodium berghei lebih baik sebagai antiplasmodium dibandingkan sebagai imunostimulan.
In-vivo antimalarial activity of Holothuria scabra simplicia in Plasmodium berghei-infected mice Sri Laksemi, Dewa Ayu Agus; Asri Damayanti, Putu Ayu; Sudarmaja, I Made; Tunas, I Ketut; Ratna Sundari, Luh Putu; Rustini, Ni Luh; Budi Apsari, Putu Indah
Universa Medicina Vol. 43 No. 2 (2024)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2024.v43.195-201

Abstract

BACKGROUNDMalaria remains one of the major causes of death worldwide. Holothuria scabra has a high nutritional content and many biological effects. The development of alternative anti-malarial drugs is necessary, considering that resistance to the newest anti-malarial drugs has been reported. This research study aimed to determine the anti-malarial effects of Holothuria scabra in mice infected with Plasmodium berghei. METHODSThis was a post-test only control group study conducted on 24 Balb/c mice. Peter’s four-day suppressive test was employed to ascertain the claimed anti-plasmodial effect of the simplicia. Following inoculation with P. berghei, all 24 mice were infected and randomized into 4 groups, namely 3 treatment groups and 1 control group. The control group was given carboxymethyl cellulose, two of the treatment groups were given doses of 10 and 100 mg/kg BW Holothuria scabra, respectively, using Peter’s four-day suppressive test, while the remaining treatment group received a dose of 100 mg/kgBW using the prophylactic method. Data were analyzed using One way ANOVA. RESULTSThe results showed by using both the four-day suppressive test and the prophylactic method, that Holothuria scabra has antimalarial activity. Holothuria scabra at a dose of 100 mg/kg BW was significantly effective in decreasing the percentage of parasitemia (p=0.000) and tended to inhibit the growth of Plasmodium berghei in mice (p=0.054). CONCLUSIONThis study demonstrated that Holothuria scabra possesses anti-plasmodial activity in mice. Hence, the sea cucumber could serve as a potential source of a newer antimalarial agent.
Antimalarial flavonoid glycoside from Carica papaya with inhibitory potential against Plasmodium falciparum dihydrofolate reductase thymidylate synthase: an in-silico study Sri Laksemi, Dewa Ayu Agus; Primayanti, I Dewa Ayu Inten Dwi; Surudarma, I Wayan; Damayanti, Putu Ayu Asri; Susanti, Ni Made Pitri
Universa Medicina Vol. 44 No. 1 (2025)
Publisher : Faculty of Medicine, Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/UnivMed.2025.v44.26-33

Abstract

BACKGROUNDCarica papaya is traditionally used to treat malaria. The mechanism of action of the active constituents may be determined by molecular docking. This study therefore examined the in silico antimalarial activity of selected compounds from Carica papaya using Plasmodium falciparum dihydrofolate reductase thymidylate synthase (PfDHFR-TS) as target protein. METHODSAntimalarial activity screening of Carica papaya compounds was done in silico using AutoDock 4.2 software which was equipped with Autodock tools 1.5.6 for preparation. Five compounds contained in Carica papaya leaves, i.e. quercitrin, isoquercitrin, carpaine, caricaxanthin, and violaxanthin were successfully docked with the target protein. The molecular docking method is declared valid if the RMSD obtained is not more than 2 Å. In vitro evaluation of the test compounds as antimalarials was accomplished by determining their inhibitory activity against dihydrofolate reductase thymidylate synthase (PfDHFR-TS) which plays a role in the synthesis of nucleotides needed by Plasmodium falciparum. RESULTSValidation of Plasmodium falciparum DHFR-TS with PDB ID 1J3I showed an RMSD value of 1.58 Å. The docking results showed that quercitrin, isoquercitrin, carpaine, and caricaxanthin showed negative energy values similar to the native ligand. Therefore the four compounds have good affinity for the target protein, while violaxanthin shows a positive energy value, indicating no affinity for the target protein. CONCLUSIONBased on binding affinity values and molecular interactions, isoquercitrin and quercitrin have inhibitory activity against dihydrofolate reductase thymidylate synthase (PfDHFR-TS), such that they have potential as natural antimalarial candidates.
Co-Authors Bisma Krisnanda, I Kadek Dana, I Kadek Eka Diarthini, Ni Luh Putu I Dewa Ayu Inten Dwi Primayanti, I Dewa Ayu Inten Dwi I Ketut Tunas I Made Sudarmaja Luh Putu Ratna Sundari Ni Luh Putu Eka Diarthini, Ni Luh Putu Eka Ni Luh Rustini Ni Made Pitri Susanti Putu Ayu Asri Damayanti Putu Indah Budi Apsari Semara, I Nyoman Sudha Sujaya, I Made Pranawa Yogananda Surudarma, I Wayan Swastika, I Nengah Raka