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All Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Mukhtabar: Journal of Medical Laboratory Technology
Arham, Chaerul
Politeknik Kesehatan Muhammadiyah Makassar, Jl. Ratulangi No. 101, Makassar, Indonesia, 90222
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Environmental Science Health Professions Immunology & microbiology

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Detection of rtxA Gene as a Biomarker of Seafood-Borne Pathogen Vibrio cholerae using In Silico PCR Assay Stalis Norma Ethica; Nur Hidayati; Hayatun Fuad; Chaerul Arham; Rivana Ariyadi; Ellyka Purwaningrum; Kazi Mohammad Zillur Rahman
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 15, No 2 (2020): August 2020
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v15i2.417

Abstract

Seafood-borne outbreaks caused by Vibrio cholerae have led to the increased need for food safety risk assessment of marine products. An in silico investigation about the potential of virulence gene of V. cholerae, rtxA, as a DNA biomarker of the toxigenic bacterium has been carried out. The aim of this study was to use the bacterial DNA biomarker sequence as a tool to facilitate early rapid detection of cholera infection. Five specific pairs of primers were designed from the rtxA open reading frame DNA of V. cholerae O1 biovar El Tor str. N16961 genomic DNA using Primer3Plus. Next, in silico Polymerase Chain Reaction (PCR) assay was carried out using the newly designed primers and 25 genomic DNA of vibrio spp. retrieved from the in silico database. One of the five designed pairs of primers, RtxAOF-RtxAOR: ‘5-CGCAAAACAGTTTCAGCCGA-3’ and 5’-AGGTTGGTCTTTTGTGGCCA-3’, could result in single DNA amplicon sized 518 bp only from V. cholerae species. No amplicon bands were produced from 17 other vibrio genomes studied using similar RtxAF-RtxAR primers. A further check showed that the amplicon was indeed part of the rtxA gene of V. cholerae. Based on this in silico study, rtxA gene appeared to be a DNA biomarker of V. cholerae, which is potential to facilitate rapid diagnosis of the virulence bacterium using in silico PCR assay.
Effectiveness of Lime Concentration As A Modification of Turk's Reagent in Leucocyte Type Count Examination Arham, Chaerul; Rasyid, Nur Qadri; Rianto, Rifo; Moito, Rahma
Mukhtabar Journal of Medical Laboratory Technology Vol 2 No 2 (2024): Mukhtabar: Journal of Medical Laboratory Technology (October 2024)
Publisher : LPPM STIKes Muhammadiyah Ciamis

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52221/mjmlt.v2i2.695

Abstract

Background & Objective: Turk solution has a composition of glacial acetic acid, gentian violet, and distilled water. Lime (Citrus aurantifolia S) has a pH of 2.0 which is almost the same as acetic acid. Both components are weak acids that can lyse blood cells other than white blood cells. This study aims to determine the effectiveness of lime concentration as a modification of Turk's reagent in counting the number of leukocytes and knowing the concentration of 1%, 2%, or 3% which is more effective in counting the number of leukocytes. Method: This study aims to provide an overview of the effectiveness of lime concentration as a modification of turk reagent composition in counting the number of leukocytes. The research method used is the Counting Room Method. This research was conducted in August 2024 at the clinical pathology laboratory of the Muhammadiyah Makassar Polytechnic. The number of samples in this study was 4 blood samples obtained from 4 correspondents with treatment, lime concentrations namely 1%, 2%, and 3%, and turk reagent as control. Result: At 1% concentration, the average difference in leucocyte count between the control and the sample was 1,150 cells/mm3, at 2% concentration a difference of 2,788 cells/mm3 was obtained with the control, and at 3% concentration a difference of 3,638 cells/mm3 was obtained. Conclusion: It can be concluded that the concentration of lime that is effective as a modification of Turk's reagent in counting the number of leukocytes is a concentration of 1%.
Co-Authors Ellyka Purwaningrum Hayatun Fuad Kazi Mohammad Zillur Rahman Moito, Rahma Muh. Rifo Rianto Nur Hidayati Rasyid, Nur Qadri Rivana Ariyadi Stalis Norma Ethica