Shoukat, Nadia
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Potential of Red Okra Extract (Abelmoschus esculentus L. Moench) to Restore Kidney Damage due to Sodium Nitrite Wahyuningsih, Sri Puji Astuti; Fachrisa, Amalia; Nisa’, Nabilatun; Kusuma, Baskara Wiku Adi; Shoukat, Nadia; Ahmar, Rasyidah Fauzia; Alifiyah, Na'ilah Insani
Biosaintifika: Journal of Biology & Biology Education Vol 13, No 1 (2021): April 2021
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v13i1.28141

Abstract

Sodium nitrite (NaNO2) found in vegetables, drinking water, and cured meats, can damage tissue because it is an oxidant. Plant phytochemicals such as quercetin are antioxidants. This study aimed to determine the potential of red okra pods ethanol extract (ROE) to repair kidney damage in mice (Mus musculus) induced by NaNO2. The red okra pods were extracted three times with saturated ethanol. The experiment used 36 male BALB/c mice aged 6-8 weeks and body weight of about 28 g. There are six research groups, namely, normal control, negative control (exposure to NaNO2 50 mg/kg BW), treatment of exposure to NaNO2 and administration of ROE at doses of 25, 50, 75, and 100 mg/kg BW. Sodium nitrite and ROE were given daily for 23 days by gavage. On day 24, the serum was isolated. Blood urea nitrogen (BUN) and creatinine (Cre) levels are measured to assess kidney function, as well as measuring the oxidant malondialdehyde (MDA) and the antioxidant enzyme of superoxide dismutase (SOD). The kidneys were made histological preparations and analyzed on the proximal convoluted tubule (PCT).  All data were statistically analyzed (α=0.05). This study indicated that the administration of ROE at a 100 mg/kg BW dose is the most optimal in repairing damage to the PCT with increased normal cells and reduced necrosis. Besides, it degraded BUN, Cre, and MDA levels in the serum of mice exposed to NaNO2 compared to the other treatments. All doses of ROE promoted the SOD level. ROE restore kidney tissue, especially on PCT to normal. Kidney damage due to exposure to NaNO2 preservatives can be reduced by administering ROE. ROE prevents kidney damage through an increase in antioxidant enzymes. ROE can be used as a food ingredient as a source of antioxidants, thereby reducing the impact of oxidant compounds.
INVENTARISASI PEMANFAATAN TUMBUHAN DAN RELEVANSINYA SEBAGAI SUMBER PEMBELAJARAN EKOPEDAGOGIK BERBASIS KEARIFAN LOKAL Wahyuni, Holy Ichda; Shoukat, Nadia; Romadhon, Nurhidayatullah
Didaktika Biologi: Jurnal Penelitian Pendidikan Biologi Vol 7, No 1 (2023): DIDAKTIKA BIOLOGI
Publisher : Universitas Muhammadiyah Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32502/dikbio.v7i1.5709

Abstract

Indonesia merupakan negara megabiodiversitas salah satunya adalah keberagaman tumbuhan. Tumbuhan memiliki manfaat penting dalam kehidupan masyarakat. Pemanfaatan ini penting untuk dikemas dalam bentuk inventarisasi. Inventarisasi memiliki manfaat dalam pengembangan pendidikan lingkungan atau ekopedagogi berbasis kearifan lokal. Penelitian ini bertujuan untuk mengidentifikasi inventarisasi pemanfaatan tumbuhan oleh masyarakat lokal, dan mendeskripsikan kerangka konseptual relevansi hasil inventarisasi sebagai sumber pembelajaran ekopedagogik berbasis kearifan lokal. Penelitian ini dilaksanakan di Desa Paciran, pesisir Utara Kabupaten Lamongan Jawa Timur. Penelitian ini merupakan penelitian kualitatif dengan analisis deskriptif. Pengumpulan data inventarisasi dilakukan melalui wawancara informan kunci, kemudian dilanjutkan dengan analisis relevansi inventarisasi pemanfaatan tumbuhan sebagai sumber pembelajaran ekopedagogik berbasis kearifan lokal dengan pendekatan studi pustaka. Temuan penelitian ini sebanyak 46 spesies tumbuhan dimanfaatkan oleh masyarakat lokal, didominasi pemanfaatan sebagai obat. Informasi tersebut relevan dikembangkan sebagai sumber pembelajaran ekopedagogik berbasis kearifan lokal. Langkah-langkah yang ditempuh adalah merumuskan data potensi daerah dalam hal ini adalah inventarisasi pemanfaatan tumbuhan oleh masyarakat lokal, kemudian data yang tersedia dikembangkan menjadi sumber pembelajaran ekopedagogik melalui berbagai model pengembangan. Indonesia is a megabiodiversity country, one of which is the diversity of plants. Plants have important benefits in people's lives. This utilization is important to package in the form of an inventory. Inventory has benefits in the development of environmental education or ecopedagogy based on local wisdom. This study aimed to identify an inventory of plant utilization by local society, and describe the conceptual framework for the relevance of inventory results as a source of ecopedagogic learning based on local wisdom. This research was located in Paciran Village, north coastal area of Lamongan Regency, East Java. This research was a qualitative research with descriptive analysis. Inventory data collection was carried out through interviews of key informants, then the research was continued with an analysis of the relevance of inventorying the use of plants as a source of ecopedagogic learning based on local wisdom with a literature study approach. This study resulted that 46 plants species were used by local society, dominated by medicinal use. This information was relevant to develop as a source of ecopedagogic learning based on local wisdom. The steps taken were to formulate regional potential data and in this case was an inventory of plant utilization by local society, then the available data was developed into a source of ecopedagogic learning through various development models.
A Critical Discourse Analysis of Gender Equity and Sex Education in Indonesian High School Biology Textbooks Wahyuni, Holy Ichda; Setiyawan, Radius; Budiman, Agus; Shoukat, Nadia
Indonesian Values and Character Education Journal Vol. 8 No. 1 (2025): April
Publisher : Universitas Pendidikan Ganesha

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23887/ivcej.v8i1.94909

Abstract

Biology learning materials, particularly on the reproductive system, often emphasize mechanistic and biological aspects while overlooking the importance of integrating adolescent sex education and gender equity values. This gap may perpetuate misconceptions and gender bias among students. This study aims to critically analyze the representation of gender equity and sexuality education in senior high school Biology textbooks through a critical discourse analysis framework. A qualitative research design was employed, utilizing Norman Fairclough’s three-dimensional model consisting of text analysis, discourse practice analysis, and social practice analysis. The study involved three widely used Grade XI Biology textbooks, including one government-issued and two private publishers' editions as comparative data sources. The findings reveal a uniform pattern of placing reproductive system topics at the end of the textbooks, predominantly presenting anatomical and physiological content without integrating comprehensive, inclusive, and gender-equitable sexuality education. Moreover, the textbooks demonstrate gender bias, insufficient discussions on consent, sexual violence, and unequal access to contraceptive information. This study concludes that the textbooks reproduce patriarchal ideologies and exclusive scientific discourse, failing to address adolescents' psychosocial and ethical aspects of sexuality. The findings highlight the urgent need for textbook revisions that promote comprehensive, gender-equitable, and contextually relevant sex education for high school students.