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All Journal AL KAUNIYAH Jurnal Biologi Universitas Andalas Jurnal Pendidikan Informatika dan Sains Muhammadiyah Medical Journal Kieraha Medical Journal Journal of Community Health Provision Cermin Dunia Kedokteran
Rialita, Ambar
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Veterinary

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Evaluation of Humoral Response of Emergency Unit Healthcare Workers after Third Dose of COVID-19 Vaccination Irsan, Abror; Mardhia, Mardhia; Rialita, Ambar
Muhammadiyah Medical Journal Vol 3, No 1 (2022): Muhammadiyah Medical Journal (MMJ)
Publisher : Faculty of Medicine and Health Universitas Muhammadiyah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24853/mmj.3.1.27-32

Abstract

Background:  Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), is an emerging disease that has become pandemic. Healthcare workers are vulnerable persons due to SARS-CoV-2 infection. Healthcare workers are a government priority in the COVID-19 vaccine program. Antibody titer evaluation to healthcare workers as the first liner is essential due to the risk of the job. Purposes: The study aims to assess IgG antibody humoral response in healthcare workers in the emergency unit of Anton Soedjarwo Hospital, Pontianak, before and after the third dose of the COVID-19 vaccine. Methods: This study was a descriptive study with a cohort method. Twenty-two healthcare workers in the Emergency Unit in Anton Soedjarwo Hospital, Pontianak, participated in the study. Three ml venous blood samples were collected from the participants in two periods to quantify IgG antibody titer. The first period was before the third dose of the COVID-19 vaccine, and the second period was one month after the third dose of the COVID-19 vaccine. Chemiluminescent Microparticle Immunoassay (CMIA) methods were used to quantification IgG antibodies against spike-receptor binding domain (S-RBD) protein of SARS-CoV-2 Results: One month before the third dose of the COVID-19 vaccine, the majority of IgG antibodies show seropositivity (90.91%), with most on range 50-10,000 AU/ml (54.55%). Two participants (9.09%) show IgG antibodies less than 50 AU/ml (seronegative). One month after the third vaccination dose, all participants (100%) elicit IgG antibodies. Based on the comparison of IgG antibody titer before and after the third dose of the vaccine, the majority (68.18%) showed increasing the IgG antibody titer after the third vaccine.  Conclusion: The third dose vaccine might effectively elevate immune response to SARS-CoV-2 infection.
The Relationship between Personal Hygiene and the Incidence of Scabies Disease in Students at Al-Adabiy Islamic Boarding School, Pontianak Putri, Nurfaidah Sulistiningtyas Sugirah; Rahmayanti, Rahmayanti; Rialita, Ambar
Journal of Community Health Provision Vol. 4 No. 3 (2024): Journal of Community Health Provision
Publisher : PSPP JOURNALS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55885/jchp.v4i3.454

Abstract

Scabies is a skin disease that is influenced by the environment and personal hygiene. Caused by infestation and sensitization of Sarcoptes scabiei var. hominis. Symptoms of scabies are complaints of itching at night, with clinical signs of papules or vesicles, the peak is like a tunnel in the predilection area, grayish white, straight or curved. The objective of this study was to determine the relationship between personal hygiene and the incidence of scabies in students at the Al-Adabiy Pontianak Islamic Boarding School in 2023. The research design used in this study was observational analytic with a cross-sectional approach conducted on 60 students of Al-Adabiy Islamic Boarding School using a simple random sampling technique, hypothesis testing using Fisher's test. The results showed that respondents who had scabies were in the age range of 12-16 years (65%), were in class X (50%) and were dominated by males (60%). 85% of students had poor personal hygiene with a scabies incidence rate of 33.3%. The results of the Fisher's test obtained a significance value (sig.) of 0.023 (p <0.05). There is a relationship between personal hygiene and the incidence of scabies.
Aktivitas antijamur isolat bakteri endofit tanaman kunyit (Curcuma longa L.) terhadap penghambatan pertumbuhan Candida albicans Prasetyo, Raditia Tri; Rialita, Ambar; Mahyarudin, Mahyarudin
Jurnal Pendidikan Informatika dan Sains Vol. 13 No. 2 (2024): Jurnal Pendidikan Informatika dan Sains
Publisher : IKIP PGRI Pontianak

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31571/saintek.v13i2.7957

Abstract

Candidiasis adalah infeksi kulit yang disebabkan oleh jamur, terutama spesies Candida albicans. Sebagian besar kasus candidiasis saat ini menunjukkan resistensi terhadap obat antijamur yang ada, sehingga diperlukan pencarian senyawa metabolit baru sebagai alternatif antijamur. Bakteri endofit, mikroorganisme yang hidup di dalam jaringan tumbuhan tanpa menyebabkan kerusakan, diketahui memiliki potensi untuk menghasilkan senyawa kimia yang berfungsi sebagai antijamur. Penelitian ini bertujuan untuk mengevaluasi dan mengidentifikasi aktivitas antijamur dari mikroorganisme endofit yang diisolasi dari tanaman kunyit (Curcuma longa L.) terhadap C. albicans. Desain penelitian yang digunakan adalah deskriptif. Aktivitas antijamur terhadap C. albicans diuji menggunakan metode difusi cakram. Dari 17 isolat endofit yang diuji, 8 isolat menunjukkan aktivitas penghambatan dengan zona hambat berkisar antara 7,16 hingga 10,6 mm. Isolat yang paling potensial, yakni isolat M2, diidentifikasi memiliki kesamaan genus dengan Pseudomonas. Analisis senyawa metabolit sekunder pada isolat M2 mengungkapkan keberadaan saponin, terpenoid, dan flavonoid sebagai senyawa antijamur. Dengan demikian, isolat endofit dari tanaman kunyit terbukti memiliki potensi untuk menghambat pertumbuhan C. albicans, sehingga tanaman kunyit dapat menjadi pilihan alternatif pengobatan secara tradisional dan menjadi salah satu sumber potensial dalam perkembangan di bidang farmasi.
DETERMINAN KEJADIAN DEMAM BERDARAH DENGUE (DBD) DI WILAYAH KOTA PONTIANAK TAHUN 2019 Zhafira, Tasya Fathia; Kahtan, M Ibnu; Rialita, Ambar
Kieraha Medical Journal Vol 7, No 1 (2025): KIERAHA MEDICAL JOURNAL
Publisher : Fakultas Kedokteran, Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/kmj.v7i1.9230

Abstract

Latar belakang: Demam Berdarah Dengue (DBD) merupakan penyakit infeksi oleh virus Dengue yang sering terjadi di Indonesia dengan kejadian luar biasa dan tingkat kematian besar. Ada banyak faktor yang berhubungan dengan kejadian DBD, diantaranya faktor pengetahuan, sikap, perilaku dan peran petugas kesehatan. Kota Pontianak merupakan salah satu daerah endemis penyakit Demam Berdarah Dengue (DBD). Tujuan: Mengetahui determinan kejadian demam berdarah dengue di wilayah Kota Pontianak Tahun 2019. Metode: Penelitian ini merupakan penelitian analitik observasional dengan pendekatan cross sectional. Sampel yang digunakan berjumlah 44 orang. Hasil: Hasil analisis didapatkan ada hubungan antara pengetahuan dan tindakan dengan kejadian DBD di Pontianak Tahun 2019 (p=0,0040,05), tidak ada hubungan antara sikap masyarakat, dukungan petugas kesehatan dan pengalaman mendapat penyuluhan dengan kejadian DBD di Pontianak Tahun 2019. Kesimpulan: Pengetahuan dan tindakan masyarakat merupakan determinan kejadian DBD di wilayah Kota Pontianak Tahun 2019.
Antifungal Activity of Endophytic Bacteria isolated from Pegagan (Centella asiatica L.) for Inhibition the Growth of Malassezia furfur Yanthi, Vivi; Mahyarudin, Mahyarudin; Rialita, Ambar
Jurnal Biologi Universitas Andalas Vol 10 No 1 (2022)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.10.1.23-32.2022

Abstract

Pityriasis versicolor is a superficial fungal infection characterized by changes in skin pigment due to stratum corneum colonization by Malassezia furfur. The Increasing of antifungal resistance and high recurrence rate, requires alternative treatment of medicinal plants. Pegagan (Centella asiatica L.) is known to produce secondary metabolites that have antifungal activity produced by endophytic bacteria that live on pegagan plant tissue. The study aimed to determine the antifungal activity of pegagan endophytic bacteria isolates to Inhibit the Growth of Malassezia furfur. The endophytic bacteria isolates were re cultured on NA media with a streak plate method. Antifungal activity test used the disk diffusion method which is characterized by the formation of inhibitory zones. Total of 37 endophytic bacteria isolates were successed re cultured, and there were 15 endophytic bacterial isolates that have potentcy as antifungal agent with inhibitory zones ranged from 6.5 to 15.52 mm. The most potential isolate was similarity with genus Pseudomonas. The secondary metabolites contained of alkaloids, terpenoids and saponins. The potential Centella asiatica endophytic bacteria had antifungal activity and similarity with the genus Pseudomonas.
Tingkat Stres Mahasiswa Program Studi Kedokteran Universitas Tanjungpura selama Pembelajaran Daring Masa COVID-19 dan Hubungannya dengan Keparahan Jerawat Pratama, Elvin Felix; Rialita, Ambar; Mahyarudin, Mahyarudin
Cermin Dunia Kedokteran Vol 51 No 1 (2024): Kedokteran Umum
Publisher : PT Kalbe Farma Tbk.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55175/cdk.v51i1.1178

Abstract

Background: During the COVID-19 pandemic, teaching and learning activities have to be conducted online. Many students have difficulty in dealing with online learning, and it may become a stress factor. Stress may cause several dermatological disorders such as acne. Objective: To determine the relationship between stress caused by online learning and acne severity during the COVID-19 period. Methods: A cross-sectional study with observational analytic research design. The population is Medical Study program students, Faculty of Medicine, Tanjungpura University. The sampling method uses the Proportionate Stratified Random Sampling technique with a minimum of 169 samples; 57 third-year students, 55 second-year students, and 57 first-year students. Stress level was measured with the MSSQ questionnaire, acne severity was measured with GAGS. Bivariate analysis used the Spearman correlation test. Results: A total of 62.7% of males and 46.8% of females experienced a moderate level of stress on online learning. A total of 66.7% of males and 56.4% of females had moderate acne. Spearman’s test for the relationship between online learning stress levels and acne severity obtained a value of 0.048 (p <0.05) and r = 0.152. Conclusion: The relationship between the stress level of online learning and the severity of acne among Medical Study program students at, the Faculty of Medicine, Tanjungpura University was very weak. Elvin Felix Pratama, Ambar Rialita, Mahyarudin. Stress Among Medical Study Program Students, Faculty of Medicine, Tanjungpura University during Online Learning in COVID-19 Period and Its Correlation with Acne Severity.
Aktivitas Antimikroba Bakteri Endofit Daun Pegagan (Centella asiatica L.) Terhadap Propionibacterium acnes Vernando, Riki; Mahyarudin, Mahyarudin; Rialita, Ambar
Al-Kauniyah: Jurnal Biologi Vol. 16 No. 1 (2023): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v16i1.20276

Abstract

 AbstrakTingginya angka kejadian jerawat dan meningkatnya resistensi terhadap antimikroba memerlukan alternatif pengobatan yang berasal dari bahan alam. Beberapa studi menunjukkan bahwa bakteri endofit tertentu memproduksi senyawa bioaktif yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tumbuhan obat. Pegagan (Centella asiatica L.) merupakan tumbuhan obat yang mempunyai banyak manfaat seperti untuk mengobati masalah kulit, menyembuhkan luka, serta menjadi agen antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba isolat bakteri endofit daun pegagan terhadap Propionibacterium acnes. Pengujian aktivitas antimikroba dilakukan dengan metode difusi cakram melalui pengukuran diameter zona hambat. Bakteri endofit yang didapat dari penelitian sebelumnya dikarakterisasi berdasarkan ciri-ciri dari morfologi koloni, morfologi sel, dan aktivitas biokimia. Sembilan belas isolat bakteri endofit memiliki aktivitas antimikroba terhadap Propionibacterium acnes, ditandai dengan terbentuknya zona hambat berdiameter antara 11,46–25,34 mm. Dua isolat yang memiliki kemampuan aktivitas antimikroba yang paling besar, yaitu isolat nomor 3 dan 17 dengan hasil identifikasi termasuk ke dalam genus Aeromicrobium. Berdasarkan hasil penelitian dapat disimpulkan bahwa bakteri endofit dari daun pegagan (Centella asiatica (L.) berpotensi sebagai antimikroba terhadap bakteri P. acnes.AbstractThe high prevalence of acne cases and the increasing resistance to antimicrobials requires alternative treatments that originate from natural ingredients. Several studies have shown that certain endophytic bacteria produce bioactive compounds that benefits human health, especially those whom isolated from medicinal plants. Gotu kola or pegagan (Centella asiatica L.) is one of medicinal plant that has many benefits such as treating skin problems, healing wounds, and also known for an antibacterial agent. The aim of the study was to determine the antimicrobial activity of the endophytic bacterial isolates of pegagan against Propionibacterium acnes. The antimicrobial activity test were carried out using the disc diffusion method by measuring the diameter of the inhibition zone within isolate. Endophytic bacteria obtained from previous studies were characterized based on the characteristics of colony morphology, cell morphology and biochemical activity. 19 isolates of endophytic bacteria showed antimicrobial activity against P. acnes, indicated by the formation of inhibition zones ranging from 11.46 to 25.34 mm. Two isolates that had the greatest antibacterial activity, namely isolates number 3 and 17 were identified and belongs to the genus Aeromicrobium. Based on the results of this study, it can be concluded that endophytic bacteria from pegagan (Centella asiatica L.) leaves has potential as an antibacterial agent against Propionibacterium acnes bacteria.
Co-Authors Abror Irsan Kahtan, M Ibnu Mahyarudin, Mahyarudin Mardhia Mardhia Prasetyo, Raditia Tri Pratama, Elvin Felix Putri, Nurfaidah Sulistiningtyas Sugirah Rahmayanti Rahmayanti Vernando, Riki Yanthi, Vivi Zhafira, Tasya Fathia