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All Journal JSFK (Jurnal Sains Farmasi & Klinis) Care : Jurnal Ilmiah Ilmu Kesehatan ARTERI : Jurnal Ilmu Kesehatan Journal of Telenursing (JOTING) Journal of Applied Nursing and Health
Ariningpraja, Rustiana Tasya
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Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Kombinasi Imajinasi Terpimpin dan Nafas Dalam terhadap Penurunan Kecemasan Pasien dengan Premature Ventricular Contraction (PVC) Kurniawati, Ninuk Dian; Sila, Nissa Aruming; Ariningpraja, Rustiana Tasya; Hariyati, Leli Ika; Suciari, Ana
Journal of Telenursing (JOTING) Vol 6 No 1 (2024): Journal of Telenursing (JOTING)
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/joting.v6i1.8948

Abstract

This study aims to explore interventions to prevent anxiety in Premature ventricular complex (PVC) patients in the ICCU treatment room. This research method uses a case study approach on three patients with PVC with almost the exact etiology and experiencing anxiety since the start of treatment and a combination of interventions carried out for three consecutive days. The results show that there are differences in anxiety parameters before and after intervention in each patient. Anxiety parameters were assessed from the first day to the third day, respectively, before the intervention and after the intervention. Conclusion: The combination of guided imagery intervention and deep breathing relaxation can help reduce anxiety in patients with PVC. Keywords: Guided imagination, Anxiety, Deep Breathing, Premature Ventricular Complex
Desinfeksi Nebulizer yang Terkontaminasi Klebsiella Pneumoniae untuk Pencegahan Ventilator-Associated Pneumonia (VAP) Ariningpraja, Rustiana Tasya; Wisnasari, Shila; Lestari, Retno; Perkasa, Wendi Genta; Suwondo, Rifka Anindita
Journal of Telenursing (JOTING) Vol 6 No 2 (2024): Journal of Telenursing (JOTING)
Publisher : Institut Penelitian Matematika, Komputer, Keperawatan, Pendidikan dan Ekonomi (IPM2KPE)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31539/joting.v6i2.10738

Abstract

This study aims to evaluate the effectiveness of nebulizer disinfection contaminated with Klebsiella pneumoniae using one of the materials widely used in hospitals, chlorhexidine. The research method used in vitro experiments. K. pneumoniae was isolated from hospital samples and then multiplied for 24 hours. The nebulizer jet was then intentionally contaminated with K. pneumoniae and disinfected using chlorhexidine solution at various concentrations. The effectiveness of the disinfection process was then assessed using culture and calculated based on the number of colonies formed after the disinfection process. The results showed that chlorhexidine was effective in significantly reducing the number of K. pneumoniae colonies, especially at a concentration of 2.5%. The use of chlorhexidine as a routine disinfectant in pneumatic nebulizers could be an effective strategy in the prevention of VAP, thus improving safety in critically ill patients. This study supports the use of chlorhexidine as part of a disinfection protocol to reduce the incidence of VAP caused by K. pneumoniae. In conclusion, chlorhexidine is effective in reducing the number of K. pneumoniae colonies so that it can be a disinfectant to prevent Ventilator-Associated Pneumonia (VAP). Keywords : Disinfectant, Jet Nebulizer, Klebsiella pneumoniae, Ventilator-Associated Pneumonia
Efikasi Bakterisidal Klorheksidin terhadap Escherichia coli dan Klebsiella pneumoniae Penghasil ESBL Ariningpraja, Rustiana Tasya; Lestari, Retno; Susanto, Akhiyan Hadi; Salam, Mohammad Nurus; Asih, Setya; Febriano, Luis Figo
ARTERI : Jurnal Ilmu Kesehatan Vol 6 No 3 (1): Mei
Publisher : Puslitbang Sinergis Asa Professional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37148/arteri.v6i3.635

Abstract

Nosocomial infections caused by multidrug-resistant bacteria, such as Escherichia coli and Klebsiella pneumoniae producing Extended-Spectrum Beta-Lactamases (ESBL), present a major challenge in healthcare. These bacteria reduce the effectiveness of antibiotics, worsening patient conditions. This study aims to evaluate the effectiveness of chlorhexidine in disinfecting medical devices contaminated with ESBL-producing bacteria. The methodology involves inoculating bacterial strains on plastic medical device surfaces and disinfecting them with chlorhexidine concentrations of 2.5%, 5%, and 7.5% for 10 minutes, with control groups using sterile water and 70% alcohol. The results indicate that chlorhexidine inhibited the colonies of E. coli and K. pneumoniae effectively, with the highest inhibition occurring at 7.5% for E. coli and at 2.5% for K. pneumoniae. This indicates that these bacteria respond differently to higher levels of chlorhexidine. Through cell lysis and membrane disruption, chlorhexidine shows vast promise as a disinfectant for medical devices against healthcare-associated infections.
Evaluating the Effectiveness of Chlorhexidine in Reducing Methicillin-Resistant Staphylococcus Aureus (MRSA) Contamination in Nebulizer Equipment Wisnasari, Shila; Ariningpraja, Rustiana Tasya; Lestari, Retno; Ahsan, Ahsan; Effendy, Nuraeni
Care : Jurnal Ilmiah Ilmu Kesehatan Vol 13, No 2 (2025): EDITION JULY 2025
Publisher : Universitas Tribhuwana Tunggadewi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33366/jc.v13i2.6313

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is a significant healthcare-associated pathogen, capable of spreading through contaminated aerosols generated by medical equipment such as nebulizers. The increasing prevalence of MRSA infections, coupled with challenges in effective disinfection, highlights the urgency of identifying optimal disinfection methods. This study aims to assess the effectiveness of chlorhexidine in reducing MRSA contamination in nebulizer equipment, focusing on determining the optimal concentration for disinfecting nebulizers to minimize airborne transmission. This study employed an in vitro experimental design. The study population consisted of MRSA isolates obtained from clinical sputum specimens. The samples included nebulizer equipment inoculated with MRSA suspensions standardized to 10⁷ CFU/mL. Disinfection was carried out using chlorhexidine at concentrations of 2.5%, 4%, and 5%, each diluted in 70% alcohol. Control groups were rinsed with sterile saline and 70% alcohol. Post-disinfection, the nebulizer surfaces were swabbed and cultured on Mueller-Hinton agar to quantify colony-forming units (CFUs). Data were analyzed using paired t-tests and one-way ANOVA followed by Tukey’s post-hoc test, with a significance level set at p 0.05. The results show that chlorhexidine 2.5% reduced bacterial counts to an average of 1 CFU/plate, and 4% chlorhexidine reduced it to 49 CFU/plate. Chlorhexidine 5% resulted in an average of 1,430.6625 CFU/plate. Statistical analysis revealed significant reductions with 2.5% and 4% chlorhexidine compared to controls (p0.01), whereas 5% Chlorhexidine did not show a significant difference. These findings suggest that lower concentrations of chlorhexidine may be sufficient for effective disinfection in nebulizer equipment, addressing the risk of MRSA spread through airborne particles.
Resilience, Psychological Well-Being, and Academic Success in Blended Learning: A Cross-Sectional Study among Healthcare Students Effendy, Nuraeni; Ariningpraja, Rustiana Tasya; Lestari, Retno
Journal of Applied Nursing and Health Vol. 7 No. 3 (2025): Journal of Applied Nursing and Health
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/janh.v7i3.275

Abstract

Background: Students in healthcare are expected to face high clinical and academic stressors that may influence their psychological well-being and academic achievement. Resilience plays a significant role in helping students navigate these challenges while maintaining their well-being. The research examines the relationship between psychological well-being, resilience, and academic achievement among Universitas Brawijaya healthcare students in a blended learning environment. Methods: A cross-sectional research design was employed, and 302 students participated through purposive sampling. Data were collected through the Academic Resilience Scale (ARS-30) and Ryff's Psychological Well-Being Scale, with GPA as an indicator of academic performance. This study was reported in accordance with the STROBE guidelines. Results: A strong positive relationship between resilience and psychological well-being (r = 0.615, p < 0.01), highlighting the focus on resilience as an effective protective factor. There was, nonetheless, no significant correlation between resilience and GPA (r = -0.006, p = 0.913).. Conclusion: Among the psychological well-being factors, environmental mastery, autonomy, and purpose in life contributed significantly to academic resilience. These findings suggest that cultivating psychological well-being, particularly ecological mastery and self-acceptance, can enable resilience in online learning environments.
An An Assessment of the Efficacy of Chlorhexidine in Nebulizer Disinfectant to Prevent Contaminated Aerosol Administration Ariningpraja, Rustiana Tasya; Wisnasari, Shila; Ahsan, Ahsan; Kristianto, Heri; Febriano, Luis Figo
JSFK (Jurnal Sains Farmasi & Klinis) Vol 12 No 3 (2025): J Sains Farm Klin 12(3), December 2025
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.12.3.186-191.2025

Abstract

Nebulization therapy is an essential method of drug administration that enables effective delivery of medicationsto the respiratory tract in aerosol form; however, contaminated nebulizer components may serve as a source of pathogenicmicroorganisms associated with nosocomial pneumonia. This in vitro experimental study aimed to evaluate the effectiveness ofchlorhexidine as a nebulizer disinfectant in eliminating pathogenic bacteria. The study employed a post-test control group designusing six groups of nebulizer chambers, consisting of two control groups and four intervention groups. Two clinically relevantbacteria, Pseudomonas aeruginosa and Acinetobacter baumannii, were inoculated into the nebulizer chambers. The interventiongroups were disinfected using chlorhexidine gluconate at concentrations of 2.5%, 4%, and 5% diluted in 70% alcohol, and 2.5%chlorhexidine diluted in distilled water, while the control groups received sterile water and 70% alcohol, in accordance withexisting guidelines. After a standardized exposure period, bacterial growth was assessed using Colony Forming Unit (CFU) counts. Data were analyzed descriptively and comparatively to evaluate bacterial eradication across groups. The results demonstrated that 5% chlorhexidine diluted in 70% alcohol achieved complete bacterial elimination (0 CFU) for both bacterial strains, whereas lower concentrations showed residual growth. These findings indicate that chlorhexidine, particularly at higher concentrations, demonstrates strong disinfectant activity against common nosocomial pneumonia pathogens in nebulizer chambers. Nevertheless, the results are limited to in vitro conditions; therefore, further studies involving a wider range of microorganisms, standardized exposure times, and assessments of aerosol contamination during clinical nebulization are warranted to support its practical application.
Effectiveness of Citrus sinensis Aromatherapy and Nature Sound Therapy on Pain, Vital Signs, and Postoperative Nausea and Vomiting: A Quasi-Experimental Study Ariningpraja, Rustiana Tasya; Fatma, Endah Panca Lydia; Lestari, Retno; Hidayati, Laely; Febriano, Luis Figo
Journal of Applied Nursing and Health Vol. 8 No. 1 (2026): Journal of Applied Nursing and Health
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/janh.v8i1.446

Abstract

Background: Postoperative recovery in the early hours after surgery is often accompanied by pain, vital-sign fluctuations, and postoperative nausea and vomiting (PONV). Complementary therapies such as Citrus sinensis aromatherapy and nature sound therapy have been used as adjuncts to pharmacological management; however, evidence on their combined effects on multiple recovery indicators in the early postoperative period remains limited. This study evaluated the effectiveness of these interventions compared with standard postoperative care. Methods: Three-group pretest–posttest quasi-experimental study was conducted in two hospitals using purposive sampling. Participants were conscious and hemodynamically stable adult patients 8–12 hours after surgery. Patients with chronic pain, hearing impairment, or olfactory dysfunction were excluded. Based on G*Power analysis (f = 0.25, α = 0.05, power = 0.80), 30 participants per group were required; therefore, 32 were recruited per group. Interventions were provided 8–12 hours post-surgery alongside standard care: Citrus sinensis aromatherapy (25 minutes) and nature sound therapy (30 minutes). Outcomes included pain intensity (Numeric Rating Scale), vital signs, and PONV severity (4-point scale). Data were analyzed using repeated-measures ANOVA in SPSS version 26. Results: Both Citrus sinensis aromatherapy and nature sound therapy showed significant improvements in pain, vital-sign stability, and reduced PONV severity compared with the control condition (p < 0.05). No adverse effects were observed. Aromatherapy demonstrated a moderate effect on PONV reduction, while nature sound therapy showed a large effect on pain reduction and vital-sign stabilization. Conclusion: Citrus sinensis aromatherapy and nature sound therapy are safe adjunctive interventions that may enhance early postoperative recovery by improving symptom burden and supporting physiological stability, with strong potential for integration into sensory-based postoperative comfort protocols.
Effect of Citrus Sinensis Aromatherapy on Pain and Anxiety Among Hemodialysis Patients: A Quasi-Experimental Study Ariningpraja, Rustiana Tasya; Fatma, Endah Panca Lydia; Lestari, Retno
Journal of Applied Nursing and Health Vol. 8 No. 1 (2026): Journal of Applied Nursing and Health
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/janh.v8i1.447

Abstract

Background: Pain is a common problem in hemodialysis patients and is often accompanied by anxiety. Aromatherapy is a simple, low-cost complementary intervention, but evidence in hemodialysis patients is still limited and inconclusive. Citrus sinensis essential oil is of particular interest because its major bioactive components are analgesic and anxiolytic agents. The purpose of this study was to determine the effect of citrus sinensis aromatherapy on pain and anxiety in hemodialysis patients. Methods: A quasi-experimental design with a control group included 50 hemodialysis patients (25 control and 25 intervention) selected by purposive sampling at the Bangil Health Centre in Pasuruan City. Eligible patients were ≥ 18 years old, have good sense of smell, on routine hemodialysis. Pain was measured using the Numeric Rating Scale (NRS) and anxiety using the Generalized Anxiety Disorder-7 (GAD-7)—data analyzed with a paired t-test. The intervention was given twice a week for 30 minutes. Ethical clearance was obtained. Results: In the intervention group, mean pain score decreased from 6.2 ± 1.1 to 3.4 ± 1.0, and anxiety scores decreased significantly (both p < 0.001), while changes in the control group were not significant. Between-group comparisons showed a greater reduction in pain and anxiety in the intervention group (p < 0.05). Conclusion: The Citrus sinensis aromatherapy was effective to reduce on pain scale and anxiety level in chronic kidney disease patients undergoing hemodialysis. These findings suggest that citrus sinensis aromatherapy can be considered as a simple complementary nursing intervention to improve comfort and psychological well-being during hemodyalisis period.
Pre-Clinical Laboratory Evaluation of Chlorhexidine for Disinfection of Semi-Critical Respiratory Equipment in Nursing Practice Ariningpraja, Rustiana Tasya; Ulfa, Marina; Jayadi, Lukky; Maslukha, Andini; Effendy, Nuraeni
Journal of Applied Nursing and Health Vol. 8 No. 1 (2026): Journal of Applied Nursing and Health
Publisher : Chakra Brahmanda Lentera Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55018/janh.v8i1.546

Abstract

Background: Healthcare-associated infections (HAIs) remain a persistent challenge in nursing practice, particularly in the reprocessing of semi-critical respiratory equipment. Although chlorhexidine is widely used as an antiseptic, evidence regarding its pre-clinical disinfectant performance, physicochemical suitability, and waste safety within nurse-led device reprocessing workflows remains limited. This gap is especially relevant in settings where reusable respiratory devices are routinely handled by nurses. Methods: This study employed a pre-clinical experimental laboratory design. The independent variable was chlorhexidine concentration, while dependent variables included antimicrobial efficacy (phenol coefficient), physicochemical parameters (pH, specific gravity, viscosity), and acute toxicity. Antimicrobial testing was conducted against Staphylococcus aureus and Pseudomonas aeruginosa. Physicochemical assessments followed standardized laboratory procedures, and acute toxicity was evaluated using Danio rerio (zebrafish) larvae to inform waste disposal safety. Expert validation was conducted using the Content Validity Index (CVI). Descriptive and endpoint-based analyses were applied without inferential statistics. Results: Chlorhexidine demonstrated strong bactericidal activity against both test organisms, with phenol coefficient values indicating effective disinfectant performance. Physicochemical characteristics remained within acceptable ranges for semi-critical respiratory device reprocessing. Toxicity assessment showed dose-dependent larval mortality, indicating the need for dilution before waste discharge. Expert validation identified chlorhexidine 7.5% as the most clinically relevant concentration for nurse-led practice. No p-values were applied due to the endpoint-based experimental design. Conclusion: Chlorhexidine shows effective pre-clinical disinfectant potential for semi-critical respiratory equipment within controlled laboratory conditions. Its application in nursing practice should be accompanied by standardized concentration control and mandatory dilution before disposal to mitigate environmental toxicity. Further clinical and field-based validation is recommended.
Co-Authors Ahsan Ahsan Asih, Setya Effendy, Nuraeni Fatma, Endah Panca Lydia Febriano, Luis Figo Hariyati, Leli Ika Heri Kristianto Hidayati, Laely Kurniawati, Ninuk Dian Lukky jayadi Maslukha, Andini Perkasa, Wendi Genta Retno Lestari Salam, Mohammad Nurus Sila, Nissa Aruming Suciari, Ana Susanto, Akhiyan Hadi Suwondo, Rifka Anindita Ulfa, Marina Wisnasari, Shila