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SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT: SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT Rizky, Vincentia Ade; Siregar, Saadah; Rahayu, Asvia; Krisdianilo, Visensius
JURNAL PENGMAS KESTRA (JPK) Vol. 2 No. 2 (2022): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Broiler chicken meat is a food source of animal origin that is widely consumed in Indonesia. The affordable price makes the demand for broiler chicken meat increasing. Chicken meat is easily contaminated by various microorganisms from the surrounding environment. Several types of microbes that can contaminate meat include E. Coli bacteria, Clostridium botulinum bacteria, Clostridium Perfringens, Salmonella bacteria, Staphylococcus aureus, and Anthrax. Microbial contamination in the form of Salmonella bacteria is generally found in broiler chicken meat. To overcome microbial contamination, the researchers conducted research on the effect of ginger on reducing the number of bacteria in chicken meat. This socialization aims to provide information to the public to be able to use ginger as an inhibitor and reduce the number of pathogenic bacteria and food destroyers in chicken meat. The results of this socialization were obtained from previous research which concluded that a decrease in the number of bacteria was due to the administration of ginger. This is because ginger contains secondary compounds such as phenols, treponoids and benzaldehyde which are bacteriostatic so that they function to inhibit the growth of microorganisms. The results of the socialization carried out in the community around Lubuk Pakam stated that they understood the processing and storage techniques (100%).

Workshop Diagnosis of Escherichia coli on Well Water Samples Krisdianilo, Visensius; Vincentia Ade Rizky; Saadah Siregar; Rahayu, Asvia
JURNAL PENGMAS KESTRA (JPK) Vol. 3 No. 1 (2023): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v3i1.1725

Diarrhea is an endemic disease in Indonesia and is also a disease that can potentially cause extraordinary events that are often accompanied by death. Escherichia coli is a commensal bacterium, intestinal pathogen and extraintestinal pathogen that can cause urinary tract infections, meningitis, and septicemia. Most of the E. coli bacteria are in the digestive tract of animals as well as humans and are normal flora, but some are pathogenic that can cause diarrhea in humans (Bettelheim, 2000). Water is very potential in the spread of various diseases, for example water contaminated by pathogens and drunk by humans can cause disease (water born disease). Food and water contamination has been identified as a potential source of the spread of Escherichia coli pathogenicity in humans. The method has the disadvantage of taking a long time, a large number of samples, and an incorrect reading method of results. PCR technique is one of the molecular techniques used to identify infectious diseases caused by Escherchia coli. This method has many advantages that can produce accurate, fast, specific product amplification, requiring a small number of samples. and this method can be used to overcome the weaknesses of conventional diagnostics (cultures). The purpose of this study was to socialize the detection of Escherichia coli bacteria in well water samples by culture and PCR methods. From the examination that has been done obtained the results of cultural examination obtained colonies that grow on the medium Mac Conkey To appear round, flat edges, smooth surface with the color of the colony, then the results of pcr examination obtained 239 bp, which means bacteria found from the isolation of well water samples in the bottom of the identic pakam with Escherichia coli.As well as workshop participants can follow and understand the materials submitted and also the examination procedures carried out.

Workshop on The Use of Miana Leaf Juice (Coleus Scutellarioides (L) Benth) as An Alternative to Safranin Dye in Bacterial Gram Staining Krisdianilo, Visensius; Siregar, Sa'adah; Rahayu, Asvia; Rizky, Vincentia Ade
JURNAL PENGMAS KESTRA (JPK) Vol. 4 No. 1 (2024): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v4i1.2198

One of the commonly used stains in bacterial identification is gram staining, this staining is used to distinguish the physical properties of the cell wall and the chemical properties of bacteria, this dye will distinguish bacteria into gram-positive bacteria with the result of the bacteria will be purple and gram negative with the result of red bacteria. The use of safranin is effective in working on bacteria because it is alkaline, this can cause binding between the chromophore component in the dye and the cytoplasm of bacteria that are basophilic. However, the use of safranin also has a negative impact on health and the environment. Therefore, the need to use materials that are safer for health and the environment, miana leaf juice (Coleus Scutellarioides (L) Benth) can be used as a natural dye to replace safranin. Minana leaves are very easy to find in gardens and are usually ornamental plants, the anthocyanin content in this plant can be used as a color pigment to replace safranin, anthocyanins will produce red to dark red pigments in an acidic atmosphere. The purpose of this community service activity, the community service organized a workshop for students of the Medical Laboratory Technology Study Program, Lubuk Pakam Medical Health Institute, aiming to provide information on the use of miana leaf juice (Coleus scutellarioides (L) Benth) as an alternative to safranin dye in bacterial gram staining. From the results of the service, it can be concluded that the participants can understand the material and the demonstration, this can be seen from the increase in the percentage of understanding both from before 61% to 87% after being given the demonstration and material.

IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Rizky, Vincentia Ade; Siregar, Saâ€™adah; Krisdianilo, Visensius; Rahayu, Asvia; Syafrina Ginting, Suventi; ., Kartini
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.615

Escherichia coli O157: H7 is the main cause of foodborne disease in several countries, one of which is diarrhea. Diarrheal disease is still a major problem in Indonesia that needs treatment and study from various aspects. The conventional method of laboratory examination such as culture is a method that is often carried out, but in making the diagnosis requires a long time, the number of samples is large, and the results are less accurate because contamination can occur. Another more accurate technique for detecting Escherichia coli O157: H7 is the PCR technique. This study aims to identify the Escherichia coli O157: H7 bacteria by culture method and PCR. The results showed that the culture method and PCR of 8 isolated samples 4 showed positive results for the bacterium Escherichia coli O157: H7. However, the PCR method is more selective and faster than the culture method.

EVALUASI PENGGUNAAN OBAT ASAM URAT DAN POLA PERESEPANNYA PADA PASIEN GOUT ARTRITIS DI INSTALASI RAWAT INAP DI RSUD DELI SERDANG LUBUK PAKAM TAHUN 2020 Rahayu, Asvia; Anna Teresia Marbun, Romauli; Nopita Sari Manalu, Dian; Siregar, Saâ€™adah; Ade Rizky, Vincentia; Krisdianilo, Visensius
Jurnal FARMASIMED (JFM) Vol 3 No 2 (2021): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i2.681

Uric acid is a disease that arises due to excess purine in the body caused by uric crystals in the joint tissue that attacks the fingers, elbows, knees, toes, toes and heels. Gouty arthritis is a progressive disease caused by deposition of monosodium urate crystals (MSU) found in the joints, kidneys, and other connective tissue due to chronic hyperuricemia. The incidence of gouty arthritis is common in men as many as 25 patients (65.8%) and women as many as 13 patients (34.2%). The incidence of gouty arthritis continues to increase with age, at the age of 21-35 years (7.9%), ages 36-55 years (36.7%), age 56-65 years (28.9%), and at the age of 66 -75 years (26.3%). The purpose of this study was to examine the use of uric acid and its prescription patterns in arthritis patients in Deli Serdang Lubuk Pakam Hospital in 2020 in terms of the right parameters according to the indications, the right patients, the right drugs, and the right dosages. This research is a non-experimental study, data retrospective retrieval was done by looking at the medical record data of arthritis gout patients and analyzed descriptively. Sampling as many as 38 patients with puposive sampling method. Inclusion criteria in this study were patients who were diagnosed with gout and given arthritis medication with complete medical record data. The results of the study evaluated the use of gout and its prescribing patterns in 38 patients at Deli Serdang Lubuk Pakam Regional Hospital in 2020, which were 100% correct, 100% right, 100% right, and 96% right.

EVALUATION OF TITER RESULTS ON WIDAL EXAMINATION BASED ON THE DURATION OF FEVER IN HOSPITAL GRANDMED LUBUK PAKAM Rahayu, Asvia; Krisdianilo, Visensius; Hutabarat, Sintya; Siregar, S'aadah; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 4 No 2 (2022): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v4i2.1010

Kultur merupakan salah satu metode yang digunakan untuk diagnosis demam tifoid, namun di beberapa daerah seringkali tidak ada fasilitas untuk kultur, maka cara lain untuk membantu menegakkan diagnosis yang praktis dan tersedia di rumah sakit adalah dengan tes Widal. Tujuan penelitian ini adalah untuk mengetahui evaluasi hasil titer pada pemeriksaan Widal berdasarkan lama demam di RS GrandMed Lubuk Pakam. Penelitian ini dilakukan di Laboratorium Patologi Klinik RS GrandMed Lubuk Pakam. Jenis penelitian ini adalah deskriptif yang dilaksanakan mulai bulan Mei 2021 sampai dengan Juni 2021. Jumlah sampel yang diperoleh sebanyak 25 orang. Dari 25 sampel tersebut, hasil uji Widal dengan titer antibodi terhadap antigen O 1:80 adalah 2 orang, 1:160 orang, 1:320 orang, 2 orang. Titer antibodi terhadap antigen H 1:80 sebanyak 2 orang, 1:160 sebanyak 4 orang, 1:320 sebanyak 2 orang, dan titer antibodi terhadap antigen AH 1:80 sebanyak 6 orang, 1:160 sebanyak 5 orang dan 1:320 sebanyak 1 orang. Kesimpulan dari penelitian ini adalah 1:160 merupakan titer paling banyak ditemukan dengan titer antibodi tertinggi terhadap antigen H yaitu 1:320 lebih sering ditemukan pada durasi demam dengan kisaran 6-9 hari sedangkan titer antibodi tertinggi terhadap antigen AH adalah 1:320 ditemukan selama penelitian. demam dengan kisaran 6-9 hari.

Modification Of Star Fruit Wuluh (Averrhoa Bilimbi L.) As A Substitute For Turk Solution In The Manual Method Leukocyte Count. Lemriani Manullang, Selda; Ade Rizky, Vincentia; Rahayu, Asvia
Medistra Medical Journal (MMJ) Vol 2 No 2 (2025): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i2.2374

Background: This study counts the number of leukocytes using a manual method. The natural materials used are lime, lemon, and tamarind, which contain glacial acid and citric acid with a pH below 2.0. These acids can lyse blood cells, except for leukocytes (white blood cells). Objective: This study aims to analyze the effectiveness of varying concentrations of starfruit (Averrhoa bilimbi) on the number of leukocytes (white blood cells). Method: This research employs an experimental method. The number of leukocytes is counted from the juice of starfruit at concentrations of 5% and 10%. The objective is to find the most effective concentration compared to the standard Turk solution as a control. Conclusion: The data were analyzed using One-Way ANOVA. The average number of leukocytes in the control group was 6,500, in the 10% concentration was 6,450, and in the 5% concentration was 4,500. The results of statistical tests and hypotheses showed a significance value of 0.000, which is less than ? (alpha) (0.05), thus the null hypothesis is rejected and there is a significant difference. The 10% concentration proved to be the most effective in replacing glacial acetic acid in the Turk solution.

Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Krisdianilo, Visensius; Rizky, Vincentia Ade; Siregar, Sa’adah; Rahayu, Asvia
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.

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