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All Journal Jurnal Kesehatan Komunitas JURNAL FARMASIMED (JFM) Jurnal Ilmiah Kesehatan Rustida Journal SAGO Gizi dan Kesehatan SEHATI: Jurnal Kesehatan Insight Power (Kekuatan Wawasan) - Jurnal Pengabdian Kepada Masyarakat Digital (JUPED) EJOIN : Jurnal Pengabdian Masyarakat Jurnal Pengmas Kestra (JPK) Jurnal Laboratorium Khatulistiwa Indonesian Journal of Community Empowerment Jurnal Sains, Teknologi dan Kesehatan Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Vincentia Ade Rizky
INKES MEDISTRA LUBUK PAKAM
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Sosialisasi Efektifitas Penggunaan Pewarna Alternatif Preparat Permanen Telur Nematoda Kolon Menggunakan Pewarna Rhodamin B Saadah Siregar; Vincentia Ade Rizky; Asvia Rahayu; Visensius Krisdianilo
Jurnal Pengabdian Kepada Masyarakat Digital Vol 1, No 3: JUPED - Agustus 2022
Publisher : Insight Power (Kekuatan Wawasan)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.358 KB)

Abstract

Staining is one way to do research to identify eggworms. The dyeing technique explains the material and description of the  worm egg under the microscope and helps to distinguish it from the surrounding feces. A widely used method for staining  histological tissue with hematoxylin and eosin. A commonly used stain for the above colon nematode examination  is eosin stain. Rhodamine B dye is used instead of eosin dye to add a reference dye. The method used is experimental and uses eosin-controlled rhodamine B concentration ratios of 0.5%, 1%, 1.5%, and 2% to 3%. The  worm egg morphology is clearly visible from the outer layer to the inner layer, the  field of view of the rhodamine B dye is clearly visible, and the color contrast of the egg  is also clearly visible. From the research conducted, it can be concluded that Rhodamine B can be used as an alternative to the dye eosin. After the seminar, participants in the dedication activity realized that dye Rhodamine B  was used instead of dye eosin.Keywords: intestinal nematode egg, hematoxylin-eosin, rhodamine B
Perbedaan hasil pemeriksaan jumlah koloni bakteri pada daging ayam broiler dengan pemberian parutan serai (Cymbopogon citratus) setelah 24 jam Saadah Siregar; Vincentia Ade Rizky; Wenda Putri Natal Saragih
Jurnal SAGO Gizi dan Kesehatan Vol 4, No 1 (2022): Juli - Desember
Publisher : Politeknik Kesehatan Kemenkes Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30867/gikes.v4i1.1073

Abstract

Background: Chicken meat is one of the most highly nutritious foodstuffs, as it contains nutrients that are indispensable to the human body, such as water, protein, fat, and other organic ingredients. However, the availability of these complete nutrients has caused the meat to become a medium for bacterial growth. Pathogenic bacteria and spoilage bacteria can cause health problems.Objectives: The study aims to determine the difference in examining the number of bacterial colonies in broiler chicken meat by giving lemongrass (Cymbopogon citratus) after 24 hours.Methods: Quantitative research using experimental design through laboratory testing, namely by determining whether or not there is a decrease in the number of germs in broiler chicken meat by giving grated lemongrass leaves after 24 hours. The sample is broiler chicken meat. The research was conducted in 2021. Laboratory testing was carried out using the pour plate method for two repetitions. Each plate of the dilution is calculated as the number of colonies by multiplying the dilution per 1 gram / 1 ml sample. Data were analyzed descriptively and bivariate using Independent T-test at 95% CI.Results: It was found that the growth of total germ numbers in chicken meat without incubation after 24 hours was treatment one was 39,96 x 106 CFU/gram, and treatment two was 39,87 x 106 CFU/gram, while chicken meat incubated for 24 hours was treatment one. The number of colonies in the first treatment was 40,39 x 106 CFU/gram, and 40,00 x 106 CFU/gram in treatment two. While chicken meat with lemongrass grater was incubated after 24 hours, there was a decrease in the number of colonies in the first treatment of 33,91 x 106 CFU/gram and the second treatment of 34,30 x 106 CFU/gram.Conclusion: There was a decrease in the number of germs in broiler chicken meat given grated lemongrass leaves after 24 hours of incubation.
Identifikasi Bakteri Coliform pada Susu Kedelai Menggunakan Metode MPN (Most Probable Number) Vincentia Ade Rizky; Saadah Siregar; Asvia Rahayu; Visensius Krisdianilo; Suci Mustika Mawardani; Dian Pratiwi
Jurnal Ilmiah Kesehatan Rustida Vol 10 No 1 (2023): Januari
Publisher : Akademi Kesehatan Rustida

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55500/jikr.v10i1.191

Abstract

Escherichia coli yaitu bakteri yang hidup diusus manusia, sebagai flora normal. Akan tetapi bakteri ini juga sering mengkontaminasi dan dapat menyebabkan penyakit pada manusia. Pengolahan pangan yang tidak hygienis dapat menyebabkan berkembangnya bakteri ini. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Coliform pada susu kedelai yang disimpan disuhu kulkas dan suhu ruangan metode MPN (Most Probable Number). penelitian ini bersifat deskriptif analitik dengan populasi susu kedelai yang disimpan disuhu kulkas dan suhu ruangan pada penyimpanan ½, 1, 1 ½, dan 2 hari. Berdasarkan hasil pengujian dengan metode presumptive test, confirmative test dan complete test didapatkan hasil pada penyimpanan disuhu kulkas penyimpanan ½ hari dan 1 hari sampel 1,2,3,4,5 yaitu <3 CFU/mL, penyimpanan 1 ½ hari sampel 1, 2 dan 3 didapatkan 15 CFU/mL, sampel 4 dan 5 yaitu 11 CFU/mL. Penyimpanan 2 hari didapatkan 20 CFU/mL. Sedangkan pada penyimpanan disuhu ruangan ½ hari sebesar <3 CFU/mL, penyimpanan 1 hari pada sampel 1,2 dan 3 yaitu 11 CFU/mL, sampel 4 dan 5 yaitu 7 CFU/mL. Penyimpanan 1 ½ hari didapatkan sampel 1,2 dan 3 yaitu 21 CFU/mL, sampel 4 dan 5 yaitu 20 CFU/mL, penyimpanan 2 hari yaitu 28 CFU/mL. Pada penyimpanan ½ hari dan 1 hari pada penyimpanan disuhu kulkas serta penyimpanan pada suhu ruangan ½ hari dinyatakan tidak ada pengaruh dan layak dikonsumsi serta memenuhi syarat mutu, Sedangkan pada penyimpanan 1 ½ hari dan 2 hari pada suhu kulkas dan 1, 1 ½, dan 2 hari pada suhu ruangan terdapat pengaruh penyimpanan dan tidak memenuhi syarat mutu konsumsi.
SEMINAR IDENTIFIKASI BAKTERI ESCHERICHIA COLI O157:H7 PADA FESES PENDERITA DIARE DENGAN METODE KULTUR DAN PCR Vincentia Ade Rizky; Sa'adah Siregar; Visensius Krisdianilo; Asvia Rahayu
JURNAL PENGMAS KESTRA (JPK) Vol 1 No 1 (2021): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.53 KB) | DOI: 10.35451/jpk.v1i1.746

Abstract

The technique of identifying Escherichia coli O157: H7 bacteria in the feces of diarrhea sufferers using a molecular method, namely PCR has been proven to be more sensitive and shows fast results but is expensive when compared to conventional methods. Therefore, it can be recommended and used by health workers in early detection so that it will help make the diagnosis faster and determine treatment more effectively. The results of this seminar aim to make it easier for laboratory staff to identify the Escherichia coli O157: H7 bacteria in diarrhea sufferers.
Pengaruh Keberadaan Jentik dan Perilaku 3M Plus terhadap Kejadian Demam Berdarah Dengue Siregar, Saadah; Mulyani, Sri; Rizky, Vincentia Ade; Akmal, Dzul; Sutriyawan, Agung
Jurnal Kesehatan Komunitas Vol 9 No 3 (2023): Jurnal Kesehatan Komunitas
Publisher : LPPM Hang Tuah Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25311/keskom.Vol9.Iss3.1392

Abstract

An infectious disease that is still a public health problem today is dengue hemorrhagic fever. Prevention efforts that can be done are by breaking the chain of transmission by eradicating mosquito nests. The purpose of this study is to analyze the influence of the presence of larvae and the behavior of 3M plus on the incidence of dengue hemorrhagic fever. The study used a cross-sectional design. The research was carried out in the working area of Puskesmas Puter, in July - August 2022. The population in this study was 7,110 heads of households. The number of samples was 67 heads of families, taken using stratified random sampling techniques. The statistical test used is the chi-square test. The results showed that the variables that affect the incidence of dengue hemorrhagic fever are the presence of larvae (p = 0.006 and POR = 4.8), closing water reservoirs (p = 0.009 and POR = 4.8), draining water reservoirs (p = 0.041 and POR = 3.5), and larvicides (p = 0.027 and POR = 4.4). This research can conclude that the incidence of DHF can be influenced by several factors, namely the presence of mosquito larvae, and the behavior of closing, draining, and sprinkling larvicide powder. It is recommended that health workers further optimize the role of levels in monitoring mosquito nest eradication behavior carried out by the community.
SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT: SOCIALIZATION OF GIVING GINGER TO REDUCING PATHOGENIC AND FOOD-DESTROYING BACTERIA IN CHICKEN MEAT Rizky, Vincentia Ade; Siregar, Saadah; Rahayu, Asvia; Krisdianilo, Visensius
JURNAL PENGMAS KESTRA (JPK) Vol. 2 No. 2 (2022): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.259 KB) | DOI: 10.35451/jpk.v2i2.1432

Abstract

Broiler chicken meat is a food source of animal origin that is widely consumed in Indonesia. The affordable price makes the demand for broiler chicken meat increasing. Chicken meat is easily contaminated by various microorganisms from the surrounding environment. Several types of microbes that can contaminate meat include E. Coli bacteria, Clostridium botulinum bacteria, Clostridium Perfringens, Salmonella bacteria, Staphylococcus aureus, and Anthrax. Microbial contamination in the form of Salmonella bacteria is generally found in broiler chicken meat. To overcome microbial contamination, the researchers conducted research on the effect of ginger on reducing the number of bacteria in chicken meat. This socialization aims to provide information to the public to be able to use ginger as an inhibitor and reduce the number of pathogenic bacteria and food destroyers in chicken meat. The results of this socialization were obtained from previous research which concluded that a decrease in the number of bacteria was due to the administration of ginger. This is because ginger contains secondary compounds such as phenols, treponoids and benzaldehyde which are bacteriostatic so that they function to inhibit the growth of microorganisms. The results of the socialization carried out in the community around Lubuk Pakam stated that they understood the processing and storage techniques (100%).
Workshop on The Use of Miana Leaf Juice (Coleus Scutellarioides (L) Benth) as An Alternative to Safranin Dye in Bacterial Gram Staining Krisdianilo, Visensius; Siregar, Sa'adah; Rahayu, Asvia; Rizky, Vincentia Ade
JURNAL PENGMAS KESTRA (JPK) Vol. 4 No. 1 (2024): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v4i1.2198

Abstract

One of the commonly used stains in bacterial identification is gram staining, this staining is used to distinguish the physical properties of the cell wall and the chemical properties of bacteria, this dye will distinguish bacteria into gram-positive bacteria with the result of the bacteria will be purple and gram negative with the result of red bacteria. The use of safranin is effective in working on bacteria because it is alkaline, this can cause binding between the chromophore component in the dye and the cytoplasm of bacteria that are basophilic. However, the use of safranin also has a negative impact on health and the environment. Therefore, the need to use materials that are safer for health and the environment, miana leaf juice (Coleus Scutellarioides (L) Benth) can be used as a natural dye to replace safranin. Minana leaves are very easy to find in gardens and are usually ornamental plants, the anthocyanin content in this plant can be used as a color pigment to replace safranin, anthocyanins will produce red to dark red pigments in an acidic atmosphere. The purpose of this community service activity, the community service organized a workshop for students of the Medical Laboratory Technology Study Program, Lubuk Pakam Medical Health Institute, aiming to provide information on the use of miana leaf juice (Coleus scutellarioides (L) Benth) as an alternative to safranin dye in bacterial gram staining. From the results of the service, it can be concluded that the participants can understand the material and the demonstration, this can be seen from the increase in the percentage of understanding both from before 61% to 87% after being given the demonstration and material.
Perbedaan Teknik Penanaman Terhadap Hasil Jumlah Koloni Bakteri Escherichia Coli Pada Suhu Inkubasi 36C: Perbedaan Teknik Penanaman Terhadap Hasil Jumlah Koloni Bakteri Escherichia Coli Pada Suhu Inkubasi 36C RIZKY, VINCENTIA ADE
Jurnal FARMASIMED (JFM) Vol 1 No 2 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.942 KB) | DOI: 10.35451/jfm.v1i2.173

Abstract

Escherichia coli bacteria are gram-negative bacteria in form of single or paired cells it is included Enterobacteriaceae family and intestinal normal flora. Laboratory tests conducted for the calculation of the number of germs can be done using cultivation techniques using a loop and micropipet. The aim of this research is to know the difference of number of bacteria bacteria on calibrated loop and micropipet to colony of Escherichia coli bacteria. This research is an analytical observation study with cross sectional design. The hypothesis was tested using independent t test with 95% confidence level. The results showed the average number colonies of Escherichia coli bacteria growing on PCA media using calibrated loop technique was 138,25 CFU/mL where as the average number of colonies of Escherichia coli bacteria grown on PCA media using micropipet technique was 104,56 CFU/mL, and significant value of p = 0,001. Furthemore, the result of data analysis showed that there were no any differences in the number of colonies of Escherichia coli bacteria in planting using calibrated loop technique and micropipet at incubation temperature of 36ºC.
EFEKTIFITAS PENGGUNAAN PEWARNA ALTERNATIF PREPARAT PERMANEN TELUR NEMATODA USUS MENGGUNAKAN PEWARNA RHODAMIN B Siregar, Saadah; Krisdianilo, Visensius; Rizky, Vincentia Ade
Jurnal FARMASIMED (JFM) Vol 2 No 1 (2019): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (243.612 KB) | DOI: 10.35451/jfm.v2i1.194

Abstract

Staining is one of support microscopic examination in the identification of worm eggs. Staining techniques are needed to clarify the various elements and the description of the microscopic worm eggs and distinguish it from the surrounding dirt. Hematoxylin and Eosin staining method that is widely used in the staining of histological tissue. Dyes commonly used in the examination of intestinal nematode eggs on is dye eosin. To added reference dye Rhodamine B dye used as an alternative to dye eosin. The method used is experiment by conducting concentration ratio of Rhodamine B 1%, 1.5%, 2% and 2.5% to 2% eosin control. The results based on Kruskal-Wallis Test showed the normality test is 0.357, p-value sig (0.357) > 0.05, so that the data group to be tested otherwise normal. Then performed statistical tests Kruskal WallisTtest to determine the accuracy of the file thus obtained p-value (0.407) > 0.05, meaning there is no significant difference in the concentration of the dye Rhodamine B of the entire treatment. Morphology of worm eggs clearly visible from the outside layer to the inside layer, the visual field Rhodamine B dye is clearly visible, color contrast eggs are also clearly visible. From the research conducted, it can be concluded that the dye Rhodamine B dye can be used as an alternative to dye eosin.
PERBANDINGAN AKTIVITAS ANTIBAKTERI INFUSA DAUN JERUK NIPIS (CITRUS AURANTIFOLIA) DAN DAUN JERUK PURUT (CITRUS HYSTRIX) TERHADAP BAKTERI ESCHERICHIA COLI Siregar, Saadah; Indriani, Indriani; Vincentia Ade Rizky, Vincentia; Visensius Krisdianilo, Visensius; Anna Teresia Marbun, Romauli
Jurnal FARMASIMED (JFM) Vol 3 No 1 (2020): Jurnal Farmasimed (JFM)
Publisher : Fakultas Farmasi Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jfm.v3i1.524

Abstract

Plant Lime (Citrus aurantifolia) and Plant Kaffir Lime (Citrus hystrix) including Rutacea family. Part of the plant lemon and lime are used as a drug other than fruit and leaves that can be used as medicine. This study aims to determine the antibacterial activity infuse lime leaves and lime leaves. Examination of the characteristics simplicia macroscopic examination. Phytochemical screening of compounds includes examining alkaloids, flavonoids, tannins and infuse saponin. Ekstrak conducted using distilled water solvent. Test of antibacterial activity against Escherichia coli bacteria carried by the agar diffusion method using a paper disc. Results obtained from macroscopic examination for lime leaves are fresh leaves leaf-shaped single smooth surface and the lower surface of the leaves are light green, the dark green upper surface. If torn, lime leaves produce coarse fibers. The leaves are small with a width of 3-5 cm. For lime leaves are fresh leaves, leaf litter pinnate compound leaves one. Child leaf blade oval to oblong, base rounded or blunt, the blunt end up pointed, 8-15 cm long, 2-6 cm wide, the upper surface colour some what shiny dark green, light green below the surface. Results of phytochemical screening simplicia powder lime leaves and lime leaves that contain a class of alkaloids, flavonoids and tannins. Antibacterial activity test results showed that there were differences between the antibacterial infusion lime leaves and lime leaves kaffir lime leaves which are more effective against the bacteria Escherichia coli compared kaffir lime leaves.
Co-Authors Adipura Atmadja Egok Akmal, Dzul Anna Teresia Marbun, Romauli Asvia Rahayu Asvia Rahayu Asvia Rahayu Avinda Sari Kanthi Rahayu Awaluddin DALIMUNTHE, HAFIKA Dian Pratiwi Eka Nurul H.P.S Hutabarat, Sintya Indriani Indriani Jayanti, Neti Eka Kartini . Krisdianilo, Vinsensius Krisdianilo, Visensius Lemriani Manullang, Selda Manurung, Arnita Elisabet Maria Selviana Joni Morischa Deyla Sitanggang Nasrullah Tamrin Nasution, Salmiah Neti Eka Jayanti Ni’mah Hidayatul Laili Nopita Sari Manalu, Dian PUTRI, IKKE ELMALIA Rachmat Saleh Rahayu, Asvia Ramadhani, Siti Riska Wahyuni Sa'adah Siregar SAADAH SIREGAR Saadah Siregar, Saadah Sa’adah Siregar Siregar, S'aadah Siregar, Sa'adah Siregar, Sa’adah Siregar, Sa’adah Sitanggang, Morischa Deyla Sri Mulyani Suci Mustika Mawardani Sutriyawan, Agung Syafrina Ginting, Suventi Syukurin Juliani Gulo Tri Astuti Sugiyatmi Umi Latifah Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo Visensius Krisdianilo, Visensius Wenda Putri Natal Saragih