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All Journal Jurnal Pengmas Kestra (JPK) Medistra Medical Journal (MMJ)
Saadah Siregar
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Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health

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Workshop Diagnosis of Escherichia coli on Well Water Samples Krisdianilo, Visensius; Vincentia Ade Rizky; Saadah Siregar; Rahayu, Asvia
JURNAL PENGMAS KESTRA (JPK) Vol. 3 No. 1 (2023): Jurnal Pengmas Kestra (JPK)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/jpk.v3i1.1725

Abstract

Diarrhea is an endemic disease in Indonesia and is also a disease that can potentially cause extraordinary events that are often accompanied by death. Escherichia coli is a commensal bacterium, intestinal pathogen and extraintestinal pathogen that can cause urinary tract infections, meningitis, and septicemia. Most of the E. coli bacteria are in the digestive tract of animals as well as humans and are normal flora, but some are pathogenic that can cause diarrhea in humans (Bettelheim, 2000). Water is very potential in the spread of various diseases, for example water contaminated by pathogens and drunk by humans can cause disease (water born disease). Food and water contamination has been identified as a potential source of the spread of Escherichia coli pathogenicity in humans. The method has the disadvantage of taking a long time, a large number of samples, and an incorrect reading method of results. PCR technique is one of the molecular techniques used to identify infectious diseases caused by Escherchia coli. This method has many advantages that can produce accurate, fast, specific product amplification, requiring a small number of samples. and this method can be used to overcome the weaknesses of conventional diagnostics (cultures). The purpose of this study was to socialize the detection of Escherichia coli bacteria in well water samples by culture and PCR methods. From the examination that has been done obtained the results of cultural examination obtained colonies that grow on the medium Mac Conkey To appear round, flat edges, smooth surface with the color of the colony, then the results of pcr examination obtained 239 bp, which means bacteria found from the isolation of well water samples in the bottom of the identic pakam with Escherichia coli.As well as workshop participants can follow and understand the materials submitted and also the examination procedures carried out.
Pewarnaan alternatif alami daun miana (Coleus scutellarioides (L) benth) sebagai pengganti gentian violet pada pewarnaan gram bakteri staphylococcus aureus VINCENTIA ADE RIZKY; SAADAH SIREGAR; VISENSIUS KRISDIANILO; RISKA WAHYUNI
Medistra Medical Journal (MMJ) Vol 1 No 2 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v1i2.2095

Abstract

Daun miana (Coleus scutellarioides (L) benth) yang memiliki corak ungu kemerahan mengindifikasikan terdapat antosianin, salah satu variannya yaitu crispa. Penelitian ini bertujuan untuk menguji zat warna antosianin pada sari daun miana sebagai pengganti gentian violet pada pewarnaan gram, Jenis penelitian yang digunakan adalah eksperimental/eksperimen. penelitian ini bertujuan untuk mengetahui apakah sari daun miana (Coleus scutellarioides (L) benth) mampu sebagai bahan alternatif pengganti reagen gentian violet pada bakteri Staphylococcus aureus. Dan pada penelitian ini dilakukan perasan sari daun miana (Coleus scutellarioides (L) benth). sampel bakteri Staphylococcus aureus dibuat sediaan preparat dan pewarnaan menggunakan gentian violet sebagai bahan kontrol dan menggunakan sari daun miana (Coleus scutellarioides (L) benth) sebagai eksperimen menggunakan konsentrasi sari daun miana yaitu 1%, 0,1% dan juga 0,5%. Data yang diperoleh diolah mengggunakan analisa data deskriftif. Hasil penelitian pada sari daun miana sari konsentrasi 1%, dan 0,1% mampu mewarnai bakteri Staphylococcus aureus dibandingan dengan konsentrasi 0,5%. Penelitian ini dapat juga dikembangkan dengan uji coba pada daun lain yang memiliki kandungan antosianin dan perlu juga melalukan konsentrasi yang lebih tinggi atau pun menggunakan metode yang lain.
Co-Authors Krisdianilo, Visensius Rahayu, Asvia Riska Wahyuni Vincentia Ade Rizky Visensius Krisdianilo