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WORKSHOP PEMANFAATAN AIR KELAPA HIJAU (cocus nucifera) SEBAGAI MEDIA IDENTIFIKASI ALTERNATIF TRIPLE SUGAR IRON AGAR (TSIA) UNTUK PERTUMBUHAN BAKTERI Escherichia coli Siregar, Sa’adah; Vincentia, Vincentia; Bintang, Siti Sarah
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/p0n9zs56

Abstract

Bacterial growth media is a material consisting of a mixture of nutrients that are used by bacteria to grow and reproduce. Biochemical tests are carried out to see the characteristics of bacteria through biochemical reactions, which are usually carried out including TSIA (Triple sugar iron agar) media which is used to identify gram-negative rod bacteria to see their ability to ferment glucose, sucrose and lactose. Based on the potential nutritional content of green coconut water, green coconut water is rich in nutrients such as potassium, protein, carbohydrates in the form of suxora (disaccharides), glucose, fructose (monosaccharides) in green coconut water, which are monosaccharides that are more easily utilized for growth as prebiotics. This type of research is experimental research. Objective: laboratory scale where this research will look at the growth of bacteria using green coconut water (Cocus Nucifera) as an alternative medium to replace Triple Sugar Iron Agar (TSIA) media. The results of the research show the growth of Escherichia coli bacterial colonies on the media. Conclusion: Green coconut water resulted in good bacterial growth. Based on the results of research on differences in the growth of Escherichia coli bacteria on TSIA (Triple Sugar Iron Agar) media and on alternative media of green coconut water (Cocus nucifera), it can be concluded that Green coconut water media can be used as an alternative medium for cultivating Escherichia coli bacteria as a substitute for TSIA.
SOSIALISASI PENINGKATAN DETEKSI SOIL TRANSMITTED HELMINTH (STH) MELALUI MODIFIKASI PADA LARUTAN ZnS (SULFIDA SENG) DAN NaCl (NATRIUM KLORIDA) 0,9% Siregar, Sa’adah; Rizky, Vincentia Ade
EJOIN : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 (2024): EJOIN : Jurnal Pengabdian Masyarakat, Juni 2024
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v2i6.2998

Abstract

Public health is a crucial aspect of disease prevention efforts. One significant health issue in many countries, including Indonesia, is Soil Transmitted Helminth (STH) infection, often caused by poor sanitation and lack of environmental cleanliness awareness. STH, typically caused by Ascaris lumbricoides, Trichuris trichiura, and hookworm, can have severe health impacts, particularly on children. Fecal examination methods, especially flotation techniques using ZnS (zinc sulfide) and 0.9% NaCl (sodium chloride) solutions, are commonly employed for STH detection. However, optimizing flotation methods in the field is essential to enhance STH detection sensitivity. This study focuses on the modification of ZnS and 0.9% NaCl solutions to improve the effectiveness of detecting STH eggs. By disseminating information on these modifications to public health practitioners, this socialization aims to enhance detection and prevention efforts for STH infections. The evaluation results show significant improvements in participants' knowledge, skills, attitudes, understanding, method application, and satisfaction, indicating the effectiveness of this socialization in promoting better STH control and prevention programs.
PENGARUH KONSENTRASI MEDIA KACANG KEDELAI(Glycine max L. Merr) DALAM PERTUMBUHAN BAKTERI Staphylococcus aureus  DIBANDINGKAN DENGAN  NUTRIENT Siregar, Sa’adah; Rizky, Vincentia Ade; Nasution, Salmiah
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1285

Abstract

Soybean is a complete protein, inexpensive and is one of the food ingredients that contain groups of essential and non-essential amino acids, carbohydrates, fats, vitamins and minerals. Staphylococcus is coccus bacteria, gram positive with an irregular arrangement or clusters like grapes. Staphylococcus aureus is a bacterium that is non-spore, non-motile, facultative anaerobe, oxidase negative and catalase positive. The aim of the study was to determine the growth of Staphylococcus aureus bacteria in alternative media for soybeans as a substance for Nutrient agar media. The type of experimental research is to conduct a trial on the concentration of soy bean powder. research  population of soybeans purchased at Lubuk Pakam market. Is an experimental method for the growth of staphylococcus aureus bacteria in soybean media as a substitute for Nutrient agar. Of research from soybeans (Glycine max L. Merr) can be used as an alternative source of protein in the manufacture of alternative media, but have slight differences in the size of the colony, the color of the colony and the color of the pigment produced with varying concentrations of 20%, 40%, 60% and 80%.  Soybeans are effectively used as an alternative protein source to replace Nutrient agar media in growing Staphylococcus aureus bacteria for a more effective concentration, namely at a concentration of 60% when viewed from the number of colonies obtained in the soybean media
WORKSHOP PENGARUH TEPUNG PISANG AMBON (Musa Paradisiaca) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Aspergillus flavus PENGGANTI MEDIA PDA (Potato Dextrose Agar) Siregar, Sa’adah; Rizky, Vincentia Ade
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 3 (2025): MAJU : Indonesian Journal of Community Empowerment, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i3.1284

Abstract

Fungi play a lot in the life both fungi that are saprophytic and are pathogenic, one that is pathogenisc and produce aflatoxin, the fungus Aspergillus flavus. PDA (Potato Dextrose Agar) is a medium commonly use for fungal growth. PDA medium are usually in the form instan so this media is ready to use. The composition of the PDA, one of which is a potato extract as a source of carbohydrates, so that other subtitutes can be carried out whose composition is almost the same as potatoes, using Ambon banana. Objective: The purpose of this research was to knew the comparison of Aspergillus flavus fungus diameter growth on PDA media and alternatif medium from ambon banana on concentration of 2%, 4% and 6%. Method: The methods this type of research was experimented by way of inoculating Aspergillus flavus with single dot method. Alternative media for ambon banana be used for the growth of the Aspergillus flavus fungus. Result: Data analysis by calculating the growth in the number of colonies growing at each contration shows that there are 85 colonies at a contration of 2%, 133 contration of 4%, and 130 contrations of 6% and there is a paired T-test abtained average results on ambon banana media with successive contrations of 2%, 4%, and 6% namely 77,33, 91,67, 123,33 and on the PDA (Potato Dextrose Agar) control media 92,33.
WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM Siregar, Sa’adah; Rizky, Vincentia Ade; Krisdianilo, Visensius; Jayanti, Neti Eka; Sitanggang, Morischa Deyla
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): EJOIN : Jurnal Pengabdian Masyarakat, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i12.1968

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.