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All Journal EJOIN : Jurnal Pengabdian Masyarakat Indonesian Journal of Community Empowerment Jurnal Sains, Teknologi dan Kesehatan Journal of Medical Surgical Concerns Medistra Medical Journal (MMJ)
Siregar, Sa’adah
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Agriculture, Biological Sciences & Forestry Humanities Computer Science & IT Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Nursing Public Health Social Sciences

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WORKSHOP PEMANFAATAN AIR KELAPA HIJAU (cocus nucifera) SEBAGAI MEDIA IDENTIFIKASI ALTERNATIF TRIPLE SUGAR IRON AGAR (TSIA) UNTUK PERTUMBUHAN BAKTERI Escherichia coli Siregar, Sa’adah; Vincentia, Vincentia; Bintang, Siti Sarah
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 1 (2025): MAJU : Indonesian Journal of Community Empowerment, Januari 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/p0n9zs56

Abstract

Bacterial growth media is a material consisting of a mixture of nutrients that are used by bacteria to grow and reproduce. Biochemical tests are carried out to see the characteristics of bacteria through biochemical reactions, which are usually carried out including TSIA (Triple sugar iron agar) media which is used to identify gram-negative rod bacteria to see their ability to ferment glucose, sucrose and lactose. Based on the potential nutritional content of green coconut water, green coconut water is rich in nutrients such as potassium, protein, carbohydrates in the form of suxora (disaccharides), glucose, fructose (monosaccharides) in green coconut water, which are monosaccharides that are more easily utilized for growth as prebiotics. This type of research is experimental research. Objective: laboratory scale where this research will look at the growth of bacteria using green coconut water (Cocus Nucifera) as an alternative medium to replace Triple Sugar Iron Agar (TSIA) media. The results of the research show the growth of Escherichia coli bacterial colonies on the media. Conclusion: Green coconut water resulted in good bacterial growth. Based on the results of research on differences in the growth of Escherichia coli bacteria on TSIA (Triple Sugar Iron Agar) media and on alternative media of green coconut water (Cocus nucifera), it can be concluded that Green coconut water media can be used as an alternative medium for cultivating Escherichia coli bacteria as a substitute for TSIA.
SOSIALISASI PENINGKATAN DETEKSI SOIL TRANSMITTED HELMINTH (STH) MELALUI MODIFIKASI PADA LARUTAN ZnS (SULFIDA SENG) DAN NaCl (NATRIUM KLORIDA) 0,9% Siregar, Sa’adah; Rizky, Vincentia Ade
EJOIN : Jurnal Pengabdian Masyarakat Vol. 2 No. 6 (2024): EJOIN : Jurnal Pengabdian Masyarakat, Juni 2024
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v2i6.2998

Abstract

Public health is a crucial aspect of disease prevention efforts. One significant health issue in many countries, including Indonesia, is Soil Transmitted Helminth (STH) infection, often caused by poor sanitation and lack of environmental cleanliness awareness. STH, typically caused by Ascaris lumbricoides, Trichuris trichiura, and hookworm, can have severe health impacts, particularly on children. Fecal examination methods, especially flotation techniques using ZnS (zinc sulfide) and 0.9% NaCl (sodium chloride) solutions, are commonly employed for STH detection. However, optimizing flotation methods in the field is essential to enhance STH detection sensitivity. This study focuses on the modification of ZnS and 0.9% NaCl solutions to improve the effectiveness of detecting STH eggs. By disseminating information on these modifications to public health practitioners, this socialization aims to enhance detection and prevention efforts for STH infections. The evaluation results show significant improvements in participants' knowledge, skills, attitudes, understanding, method application, and satisfaction, indicating the effectiveness of this socialization in promoting better STH control and prevention programs.
PENGARUH KONSENTRASI MEDIA KACANG KEDELAI(Glycine max L. Merr) DALAM PERTUMBUHAN BAKTERI Staphylococcus aureus  DIBANDINGKAN DENGAN  NUTRIENT Siregar, Sa’adah; Rizky, Vincentia Ade; Nasution, Salmiah
EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan Vol. 2 No. 2 (2025): EMPIRIS : Jurnal Sains, Teknologi dan Kesehatan, Juni 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/empiris.v2i2.1285

Abstract

Soybean is a complete protein, inexpensive and is one of the food ingredients that contain groups of essential and non-essential amino acids, carbohydrates, fats, vitamins and minerals. Staphylococcus is coccus bacteria, gram positive with an irregular arrangement or clusters like grapes. Staphylococcus aureus is a bacterium that is non-spore, non-motile, facultative anaerobe, oxidase negative and catalase positive. The aim of the study was to determine the growth of Staphylococcus aureus bacteria in alternative media for soybeans as a substance for Nutrient agar media. The type of experimental research is to conduct a trial on the concentration of soy bean powder. research  population of soybeans purchased at Lubuk Pakam market. Is an experimental method for the growth of staphylococcus aureus bacteria in soybean media as a substitute for Nutrient agar. Of research from soybeans (Glycine max L. Merr) can be used as an alternative source of protein in the manufacture of alternative media, but have slight differences in the size of the colony, the color of the colony and the color of the pigment produced with varying concentrations of 20%, 40%, 60% and 80%.  Soybeans are effectively used as an alternative protein source to replace Nutrient agar media in growing Staphylococcus aureus bacteria for a more effective concentration, namely at a concentration of 60% when viewed from the number of colonies obtained in the soybean media
Comparison Of Potato (Solanum tuerosum L) and Corn (Zea mays) as Alternative Media To Nutrient Agar Media In The Groth of Staphylococcus aureus Bacteria Rahmawati; Siregar, Sa’adah; Ade Rizky , Vincentia
Medistra Medical Journal (MMJ) Vol 2 No 1 (2024): Medistra Medical Journal (MMJ)
Publisher : Institut Kesehatan Medistra Lubuk Pakam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35451/mmj.v2i1.2376

Abstract

Background: Microbiology is the study of microorganisms, which are microscopic organisms that can only be observed through a microscope. These microorganisms have an important role in various aspects of life, including in the health sector. Objective: This study aims to evaluate whether alternative media, such as potato and corn, can be used as a substitute for Nutrient Agar in supporting the growth of Staphylococcus aureus bacteria. Methods: This research is experimental, by comparing the effectiveness of potato and corn media as an alternative to Nutrient Agar for the growth of Staphylococcus aureus. Results: The results of the research in the One-way Anova test obtained a significant value (0.00) which means (p <0.05), so it can be concluded that there is a significant difference between the ability of Nutrient Agar media, potato media and corn media in growing Staphylococcus aureus bacteria. To find out each group that has a significant difference, the Post-hoc follow-up test is carried out. The results of the post-hoc follow-up test shown in Figure 4.2.2 obtained a significant value between Nutrient Agar media and potato media (0.00), Nutrient Agar media with corn media (0.558), and between potato and corn media (0.00). Conclusion: The results showed that potato media was significantly different in its ability to grow Staphylococcus aureus compared to Nutrient Agar and corn. Potato media shows potential as an alternative to Nutrient Agar, despite its different effectiveness.
WORKSHOP PENGARUH TEPUNG PISANG AMBON (Musa Paradisiaca) SEBAGAI MEDIA ALTERNATIF PERTUMBUHAN JAMUR Aspergillus flavus PENGGANTI MEDIA PDA (Potato Dextrose Agar) Siregar, Sa’adah; Rizky, Vincentia Ade
MAJU : Indonesian Journal of Community Empowerment Vol. 2 No. 3 (2025): MAJU : Indonesian Journal of Community Empowerment, Mei 2025
Publisher : Lembaga Pendidikan dan Penelitian Manggala Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62335/maju.v2i3.1284

Abstract

Fungi play a lot in the life both fungi that are saprophytic and are pathogenic, one that is pathogenisc and produce aflatoxin, the fungus Aspergillus flavus. PDA (Potato Dextrose Agar) is a medium commonly use for fungal growth. PDA medium are usually in the form instan so this media is ready to use. The composition of the PDA, one of which is a potato extract as a source of carbohydrates, so that other subtitutes can be carried out whose composition is almost the same as potatoes, using Ambon banana. Objective: The purpose of this research was to knew the comparison of Aspergillus flavus fungus diameter growth on PDA media and alternatif medium from ambon banana on concentration of 2%, 4% and 6%. Method: The methods this type of research was experimented by way of inoculating Aspergillus flavus with single dot method. Alternative media for ambon banana be used for the growth of the Aspergillus flavus fungus. Result: Data analysis by calculating the growth in the number of colonies growing at each contration shows that there are 85 colonies at a contration of 2%, 133 contration of 4%, and 130 contrations of 6% and there is a paired T-test abtained average results on ambon banana media with successive contrations of 2%, 4%, and 6% namely 77,33, 91,67, 123,33 and on the PDA (Potato Dextrose Agar) control media 92,33.
Real time polymerase chain reaction assay (RT-PCR) sebagai tes cepat mycobacterium tuberculosis dari sampel dahak pasien tuberculosis Krisdianilo, Visensius; Rizky, Vincentia Ade; Siregar, Sa’adah; Rahayu, Asvia
JOURNAL OF Medical Surgical Concerns Vol. 4 No. 1 (2024): June Edition 2024
Publisher : Published by: Indonesian Public Health-Observer Information Forum (IPHORR) Kerjasama dengan Himpunan Perawat Medikal Bedah Indonesia (HIPMEBI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56922/msc.v4i1.371

Abstract

Background: Mycobacterium tuberculosis is known as a highly pathogenic bacterium. These bacteria are aerobic in shape like rods and have resistance to acids. This bacteria can cause tuberculosis (TB). TB disease has been widely recognized as a cause of death which is quite high in the world. Tuberculosis causes the death of nearly one million women each year. Currently, no country in the world is free from tuberculosis. Data shows that Indonesia is the third largest contributor of tuberculosis cases in the world. Examination of mycobacterium tuberculosis bacteria which is routinely carried out in hospitals or health centers uses microscopic diagnosis of acid-resistant bacilli (BTA). Recently, a rapid test of Mycobacterium tuberculosis was carried out using the semi-quantitative Real Time Polymerase Chain Reaction Assay (RT-PCR) method which targets the rpoB gene in Mycobacterium tuberculosis, which can automatically process preparations by extracting doxyribo nucleic acid (DNA) in a cartridge. The time needed for this diagnosis is approximately two hours until the results come out. Purpose: To see the results of the rapid test compared to the microscopic diagnosis at the Puskesmas. Method: Using the cross-sectional method, with a sample of 25 patients at the Lubuk Pakam Health Center. To detect Mycobacterium tuberculosis using the GeneXpert MTB/RIF tool. Furthermore, the data obtained was processed and examined using the Wilcoxon statistical test. Results: The value of the statistical test results using the Wilcoxon test showed that the p-value (Sig) was 0.264, the value was > 0.05, which means that in this study there were no significant differences in results between the microscopic method and RT-PCR. Conclusion: Overall there was no significant difference in the results between microscopy and RT-PCR methods. Keywords: Tuberculosis; Mycobacterium Tuberculosis; RT-PCR Pendahuluan: Mycobacterium tuberculosis dikenal sebagai bakteri yang sangat patogen. Bakteri ini berbentuk aerobik seperti batang dan memiliki ketahanan terhadap asam. Bakteri ini dapat menyebabkan tuberkulosis (TB). Penyakit TBC telah dikenal luas sebagai penyebab kematian yang cukup tinggi di dunia. Tuberkulosis menyebabkan kematian hampir satu juta wanita setiap tahun. Saat ini tidak ada satu negara pun di dunia yang bebas dari tuberkulosis. Data menunjukkan bahwa Indonesia merupakan penyumbang kasus tuberkulosis terbesar ketiga di dunia. Pemeriksaan bakteri mycobacterium tuberculosis yang rutin dilakukan di rumah sakit atau puskesmas menggunakan diagnosis mikroskopis acid-resistant basil (BTA). Baru-baru ini telah dilakukan uji cepat Mycobacterium tuberculosis menggunakan metode semi kuantitatif Real Time Polymerase Chain Reaction Assay (RT-PCR) yang menargetkan gen rpoB pada Mycobacterium tuberculosis, yang secara otomatis dapat memproses preparat dengan ekstraksi asam nukleat doxyribo (DNA) pada catridge. Waktu yang dibutuhkan dalam diagnosis ini adalah kurang lebih dua jam sampai hasilnya keluar. Tujuan: Untuk melihat hasil rapid test tersebut dibandingkan dengan diagnosis mikroskopis pada Puskesmas. Metode: Menggunakan metode  cross sectional, dengan sampel yaitu pasien Puskesmas Lubuk Pakam sebanyak 25 orang. Untuk mendeteksi Mycobacterium tuberculosis menggunakan alat GeneXpert MTB/RIF. Selanjutnya data yang didapat di olah dan diperiksa dengan menggunakan uji statistic Wilcoxon. Hasil: Nilai hasil uji statistic menggunakan uji Wilcoxon didapatkan hasil p-value (Sig) adalah 0.264 nilai tersebut > dari 0.05 yang berarti pada penelitian ini hasilnya tidak terdapat perbedaan hasil yang signifikan antara metode mikroskopis dengan RT-PCR. Simpulan: Secara keseluruhan tidak ada perbedaan yang signifikan dalam hasil antara metode mikroskopis dan RT-PCR.  
WORKSHOP PENGGUNAAN SARI DAUN PACAR KUKU (Lawsonia inermis L) SEBAGAI PENGGANTI SAFRANIN PADA PEWARNAAN GRAM Siregar, Sa’adah; Rizky, Vincentia Ade; Krisdianilo, Visensius; Jayanti, Neti Eka; Sitanggang, Morischa Deyla
EJOIN : Jurnal Pengabdian Masyarakat Vol. 1 No. 12 (2023): EJOIN : Jurnal Pengabdian Masyarakat, Desember 2023
Publisher : LPPM Institut Pendidikan Nusantara Global

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55681/ejoin.v1i12.1968

Abstract

Gram staining is one way to differentiate gram-positive bacteria and gram negative bacteria. Gram staining in the laboratory is done with using a solution of gentien violet, iodine, alcohol, and safranin. Leaf henna nail (Lawsonia inermis L) is a plant that produces the reddish yellow molecule is called Lawsone. Additionally leaves henna nails It also contains anthocyanins which produce a reddish orange color. This Community Service aims to inform the audience about the benefits of henna leaf extract (Lawsonia inermis L) is effectively used as a substitute for safranin in gram staining. The results of the workshop show that the audience can understand the benefits of henna nail leaf extract as a substitute for safranin in gram staining, but this is only an alternative.
Co-Authors Ade Rizky , Vincentia Bintang, Siti Sarah Jayanti, Neti Eka Krisdianilo, Visensius Nasution, Salmiah Rahayu, Asvia Rahmawati Sitanggang, Morischa Deyla Vincentia Ade Rizky Vincentia, Vincentia