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EFEKTIVITAS SENYAWA ASAM HEKSADEKANOAT DAN β-SITOSTEROL ISOLAT DARI HYDROID Aglaophenia cupressina LAMOUREOUX SEBAGAI BAHAN ANTIMIKROBA PADA BAKTERI Salmonella thypi dan JAMUR Aspergillus flavus Sjafaraenan Sjafaraenan; Eva Johannes; Mustika Tuwo
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 6 No. 1 (2021): Bioma, Januari - Juni 2021
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v6i1.13296

Abstract

Kasus cemaran mikroorganisme patogen pada bahan pangan yang dapat menimbulkan berbagai penyakit dan meningkatnya resistensi terhadap berbagai jenis antimikroba, maka diperlukan jenis antibiotik baru yang lebih efektif untuk mengatasi bakteri Multi Drug Resistant (MDR). Penelitian ini bertujuan untuk mengetahui efektivitas senyawa bioaktif dari hydroid Aglaophenia cupressina Lamoureoux dalam menghambat atau mematikan bakteri S almonlla thypi dan jamur Aspergillus flavus yang sering mencemari bahan pangan. Metode penelitian yang digunakan adalah metode experimental, dengan tahapan perlakuan; Isolasi dan karakterisasi senyawa dari hydroid A.cupressina Lamoureoux dengan metode kromatografi, elusidasi struktur molekul isolat dengan UV,IR,NMR. Dua isolat yang diperoleh yaitu asam heksadekanoat dan β-sitosterol selanjutnya diuji terhadap S. Thypi dan A. flavus dengan konsentrasi senyawa ( 30 ppm dan 50 ppm). Hasil penelitian ditemukan bahwa senyawa asam heksadekanoat dengan konsentrasi (30ppm dan 50 ppm) bersifat bakterisida terhadap S. Thypi dan bersifat fungistatik terhadap A. flavus pada konsentrasi (30 ppm dan50 ppm). β-sitosterol dengan konsentrasi (30 ppm dan 50ppm) bersifat bakteriostatik terhadap S. thypi,dan bersifat fungistatik terhadap jamur A. flavus. Kata kunci : Asam Heksadekanoat, β-sitosterol, Hydroid Aglaophenia cupressina Lamoureoux, Antimikroba, Salmonella thypi, Aspergillus flavus
Pengaruh Suplemen Organik Terhadap Regenerasi Tunas Pisang Barangan Musa acuminata Colla. Secara In Vitro Mustika Tuwo; Baharuddin Baharuddin; Andi Ilham Latunra; A. Masniawati; Tutik Kuswinanti
Metamorfosa: Journal of Biological Sciences Vol 8 No 1 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i01.p13

Abstract

The research purpose to know ability of natural supplements in supporting the regeneration of banana cv. Barangan Musa acuminata Colla. Explants were cultured on MS basal medium (control) with natural supplements: coconut water (CW) 5; 10; 15 and 20%, green bean sprout extract (GBSE) and sweet corn extract (SCE) at 50; 100; 150 and 200 g/l. After 4 weeks of cultivation, maximum multiplication shoots (7.11 ± 0.19), shoot length (2.00 ± 0.22), and number of leaves (4.00 ± 0.38) was obtained in MS + 200 g/l bean sprout extract. The addition of natural supplements to the medium showed better results on all parameters of observation than control. This results indicates the potential of natural supplements for supporting of Barangan Musa acuminata Colla. propagation in vitro culture.
Aplikasi Pupuk Hayati Mikrobat Untuk Meningkatkan Produktivitas Pertanaman Padi Desa Bulu Allaporenge Kabupaten Bone Hatta Jamil; Zainal Zainal; Muhammad Yunus; Baharuddin Baharuddin; Mustika Tuwo
Jurnal Ilmu Alam dan Lingkungan Vol. 11 No. 1 (2020): Jurnal Ilmu Alam dan Lingkungan
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.234 KB) | DOI: 10.20956/jal.v11i1.9311

Abstract

Rice is the main commodity for most agricultural communities in Indonesia and is still main food ingredient needed by around 90% of Indonesian population. Rice production in South Sulawesi varies greatly between 3 to 7 tons per hectare of dry un-husked rice in normal season conditions. Condition of food crops in Bone District is supported by paddy fields in several districts. Bone District is area with the largest rice production in South Sulawesi. The use of biological fertilizers needs to be developed in an effort to reduce the use of synthetic chemicals. Application of biological fertilizer becomes an excellent complement, because in addition to increasing soil fertility it also spurs plant growth. Application of Mikrobat biofertilizers is carried out on rice plantations located in Bulu Allaporenge Village, Bengo Sub-District, Bone District. Process of giving Mikrobat fertilizer is carried out 15-20 DAPs (days after planting). Treatment of Mikrobat biofertilizers at a dose of 1 liter per hectare at a ratio of 1: 100. The results of application of Mikrobat biofertilizer technology have a significant effect on plant height, panicle length, number of grain per panicle and weight of 1000 grains. While variable number of tillers and percentage of empty grains had no significant effect. The response of farmers is very high to apply biofertilizer technology on rice plantations because farmers immediately see the appearance of good plant growth compared to controls (without the application of microbial fertilizer).