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Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis Azhar, Rodiyah; Julianti, Elin; Natasasmita, Setiawan; Dharsono, Hendra Dian Adhita
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July
Publisher : Faculty of Dentistry Universitas Padjadjaran, Indonesia

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods: Â Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity againstÂ E. faecalis and can be further developed as a potential root canal irrigation solution.

Streamline characteristics using the computational fluid dynamic analysis in the flow of 18% EDTA irrigation solution to remove Ca(OH)2 Anna Muryani; Hendra Dian Adhita Dharsono; Z. Zuleika; Ing. Mochammad Agoes Moelyadi; Diani Prisinda
Majalah Kedokteran Gigi Indonesia Vol 4, No 2 (2018): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.30886

The remaining calcium hydroxide (Ca(OH)2) medicaments in the root canal wall can block the penetration of filling medicaments to the dentine tubule and cause the failure in the root canal treatment. One of the ways in cleaning the root canal wall from the remains of (Ca(OH)2) is by using 18% Ethylene Diamine Tetra Acetic Acid (EDTA) irrigation solution. The cleanliness of the root canal can be examined using the Computational Fluid Dynamics (CFD) analysis. The aim of this research was to see the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with 18% EDTA irrigation by means of CFD analysis. This is a descriptive-explorative research. Having been validated with the experimental research using test specimen in the form of block resin made in accordance with the characteristics of root canal, the description of the cleanliness of the root canal wall from the (Ca(OH)2) medicament with the 18% EDTA irrigation was analyzed using CFD method. The irrigation needle used was the side-vented type with the position of 3 mm from the tooth apical. The results of the research showed the conformity between the result of experimental research and CFD research. One of the results of this research was the characteristics of streamline of 18% EDTA in the root canal showing a unique behavior due to the characteristics of the form of side vent irrigation needle. The irrigation flow in the coronal area of the inlet (side vent irrigation needle) showed a low velocity causing the more fluid flowing out from the inlet went to apical rather to the outlet (root canal orifice). In conclusion, this research showed that 18% EDTA solution indicated the conformity of validation results between experimental research and CFD research in the frames of 5, 10, 15, and 20 secs observed from experimental research with the of frame 0,010, 0,099, 0,150 and 0,410 secs as observed from CFD research.

Perawatan saluran akar pada gigi molar pertama kanan mandibula dengan pulpitis ireversibel simtomatikRoot canal treatment of mandibular right first molar with symptomatic irreversible pulpitis Lydiawati Wibisono; Hendra Dian Adhita Dharsono
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 32, No 3 (2021): Februari 2021 (Suplemen 2)
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v32i3.29523

Pendahuluan: Pulpitis ireversibel simtomatik ditandai dengan hipersensitivitas terhadap stimulus termal, yang menyebabkan nyeri spontan atau rasa sakit yang bertahan 30 detik atau lebih setelah stimulus dihilangkan, nyeri yang parah, persisten, dan sulit dilokalisir, dapat menjalar ke telinga, pelipis, mata atau leher. Kondisi pulpa yang terinflamasi tidak dapat kembali pulih dan perawatan saluran akar merupakan pilihan perawatan. Tujuan laporan kasus ini membahas mengenai perawatan saluran akar dengan pulpitis ireversibel simtomatik untuk menghentikan inflamasi pulpa dan mempertahan gigi. Laporan kasus: Pasien laki-laki berusia 35 tahun datang ke klinik dengan keluhan gigi belakang kanan bawah terasa sakit berdenyut sejak 1 minggu yang lalu. Gigi tersebut pernah dilakukan penambalan sementara 4 bulan sebelumnya dan tambalan tersebut patah serta nyeri spontan. Pemeriksaan radiologis gigi 46 menunjukkan gambaran radiolusen pada bagian mahkota hingga mencapai tanduk pulpa, terdapat 2 akar, dengan akar mesial dilaserasi ke arah distal dan akar distal lurus, serta saluran akar yang menghilang pada 2/3 dan 1/3 saluran akar distal dan mesial, tidak terdapat pelebaran membran periodontal, lamina dura normal dan tidak terdapat kelainan periapikal. Gigi 46 dilakukan anestesi lokal, dilanjutkan pembukaan akses dan ekstirpasi pulpa, kemudian preparasi saluran akar menggunakan nikel titanium rotary ProTaper Next® (Maillefer, Switzerland) dengan teknik crown down dan pengisian saluran akar. Restorasi follow up overlay komposit indirek pada gigi 46. Simpulan: Pulpitis ireversibel simtomatik pada gigi molar dengan akar dilaserasi terbukti dapat ditangani dengan perawatan saluran akar yang adekuat, ditandai dengan hilangnya keluhan pada pasien dalam kasus ini.Kata kunci: Pulpitis ireversibel simtomatik, perawatan saluran akar, teknik crown down. ABSTRACTIntroduction: Symptomatic irreversible pulpitis is characterised by hypersensitivity to the thermal stimuli, which causes spontaneous pain that lasts 30 seconds or more after the stimulus removed, severe, persistent and difficult to localise, can radiate to the ears, temples, eyes, or neck. The inflamed pulp does not recover; thus, root canal treatment is the treatment option. This case report was aimed to discuss the root canal treatment for symptomatic irreversible pulpitis to stop the pulp inflammation and preserve the teeth. Case report: A 35-year-old male patient came to the clinic with complaints of pulsating pain in the mandibular right molar since one week prior. The tooth had a temporary filling four months earlier, and the filling was fractured, and the pain was spontaneous. Radiological examination of tooth 46 showed radiolucent images on the crown until the pulp horn, there were found two roots, with mesial roots dilated distally and straight distal roots, and root canals that disappeared in the two-third and one-third of the distal and mesial root canals, no dilation of the periodontal membrane, normal lamina dura and no periapical abnormalities. Tooth 46 was subjected to local anaesthesia, followed by access opening and pulp extirpation, then the root canal preparation was performed using the ProTaper Next® rotary nickel-titanium (Maillefer, Switzerland) with the crown-down technique and root canal filling. Indirect composite overlay follow-up restoration was conducted of tooth 46. Conclusion: Symptomatic irreversible pulpitis in molars with dilacerated roots proved to be manageable with adequate root canal treatment, marked by the loss of the patients’ complaints.Keywords: Symptomatic irreversible pulpitis, root canal treatment, crown-down technique.

Penatalaksanaan non-bedah kasus previously initiated therapy gigi molar pertama maksila dengan lesi periapikal dan konfigurasi dua saluran akar mesiobukal Non-surgical management of previously initiated therapy of the maxillary first molar with periapical lesions and two mesiobuccal canals configuration Diatri Nariratih; Hendra Dian Adhita Dharsono
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 3 (2021): Desember 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i3.29525

ABSTRAKPendahuluan: Penatalaksanaan kasus penyakit pulpa yang disertai dengan lesi periapikal dapat dilakukan dengan perawatan saluran akar tanpa intervensi bedah. Preparasi biomekanis pada perawatan endodontik non-bedah dapat mengeliminasi bakteri dari saluran akar dan mencegah terjadinya infeksi berulang setelah dilakukannya obturasi. Kompleksitas anatomi menyebabkan keterbatasan preparasi biomekanis saluran akar, sehingga perawatan dapat mengalami kegagalan. Variasi anatomi saluran akar pada gigi molar pertama rahang atas umumnya terdapat pada bagian palatal akar mesiobukal yang disebut sebagai saluran akar mesiobukal kedua dengan insidensi 94%. Saluran akar mesiobukal kedua berukuran lebih sempit dan dangkal dibandingkan saluran akar mesiobukal pertama, sehingga dibutuhkan beberapa metode untuk menentukan lokasi orifis tersebut. Tujuan laporan kasus ini adalah membahas mengenai penatalaksanaan non-bedah gigi pasca perawatan endodontik parsial yang disertai dengan lesi periapikal. Laporan kasus: Pasien perempuan berusia 28 tahun datang dengan keluhan gigi belakang kiri atas sakit berdenyut sejak 1 bulan, gigi tersebut pernah dirawat saluran akarnya beberapa tahun sebelumnya. Pemeriksaan klinis menunjukkan terdapat tambalan permanen pada gigi 26. Pemeriksaan radiografis menunjukkan adanya gambaran radiopak pada email mesio-oklusal hingga mencapai dasar kamar pulpa, pelebaran ligamen periodontal, terputusnya lamina dura, serta lesi periapikal pada akar mesial dan palatal. Preparasi akses dilakukan pada gigi 26, dilanjutkan dengan penentuan lokasi orifis mesiobukal kedua. Empat saluran akar dipreparasi menggunakan instrumen rotary nickel titanium dengan teknik crown down dan dilakukan obturasi teknik kondensasi lateral. Restorasi definitif berupa mahkota penuh porselen. Simpulan: Lesi periapikal sembuh setelah dilakukan preparasi pada saluran akar mesiobukal kedua sehingga pengetahuan mengenai anatomi dan variasi internal saluran akar gigi sangat penting dalam keberhasilan perawatan endodontik non-bedah.Kata kunci: Perawatan endodontik non-bedah; previously initiated therapy; lesi periapical; mesiobukal kedua ABSTRACTIntroduction: The management of pulp disease cases accompanied by periapical lesions can be done by root canal treatment without surgical intervention. Biomechanical preparations for non-surgical endodontic treatment can eliminate bacteria from the root canal and prevent re-infection after obturation. However, the complexity of the anatomy limits the biomechanical preparation of the root canal so that treatment can fail. Anatomical variations of the root canal in the maxillary first molars are generally found in the palatal part of the mesiobuccal root, which is the second mesiobuccal root canal with an incidence of 94%. The second mesiobuccal root canal is narrower and shallower than the first mesiobuccal root canal, so several methods are needed to determine the location of the orifice. The purpose of this case report was to discuss the non-surgical management of teeth after partial endodontic treatment accompanied by periapical lesions. Case report: A 28-year-old female patient complained of throbbing pain in the left upper back tooth for one month. The tooth had had its root canal treated several years before. Clinical examination revealed permanent fillings on tooth 26. Radiographic examination showed the radiopaque appearance of mesio-occlusal enamel to the floor of the pulp chamber, widening of the periodontal ligament, rupture of the lamina dura, and periapical lesions of the mesial and palatal roots. Access preparation was performed on tooth 26, followed by the determination of the location of the second mesiobuccal orifice. Four root canals were prepared using a rotary nickel titanium instrument with a crown down technique and obturation with lateral condensation technique. The definitive restoration is a full porcelain crown. Conclusions: Periapical lesions healed after preparation of the second mesiobuccal root canal. This result proves that knowledge of the anatomy and internal variations of the root canal is fundamental in the success of non-surgical endodontic treatment.Keywords: Non-surgical endodontic treatment; previously initiated therapy; periapical lesions; second mesiobuccal canal

Velocity and wall shear stress of 18% EDTA irrigation solution flow in the removal of Ca(OH)2 with computational fluid dynamic analysis Muryani, Anna; Dharsono, Hendra Dian Adhita; Zuleika, Zuleika; Moelyadi, Mochammad Agoes; Fatriadi, Fajar
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Ethylene Diamine Tetra Acetic Acid (EDTA) 18% irrigation solution is one of the chelating agents that able to remove calcium hydroxide (Ca(OH)2) from the root canal wall. Failure of root canal treatment can be caused by the presence of residual calcium hydroxide in the root canal wall, thus blocking the bond between the medication and root canal filling material with the root canal wall. This study was aimed to observe the EDTA 18% flow in removing the Ca(OH)2 using CFD analysis. Methods: This research was descriptive. Cleanliness of the root canal wall from Ca(OH)2 medicament using EDTA 18% irrigation was analysed using the CFD method with test specimens in the form of resin blocks made according to the characteristics of the root canal. The irrigation needle used was side-vented with a position of 3 mm from the apical tooth. Stage analysis of root canal geometry was performed using Computational Fluid Dynamics (CFD) analysis to observe the characteristics of irrigation solutions in root canals in 3D. Results: The streamlined characteristics of EDTA 18% irrigation solution showed a unique behaviour due to the features of the side-vent shaped irrigation needle. Irrigation flow in the crown area of the inlet (side-vented irrigation needle) showed low velocity so that the fluid flow when exiting the inlet was more towards the apical than the outlet (root canal orifice). Conclusion: Velocity and wall shear stress of EDTA 18% showed the results validation conformity between experimental and CFD, that the maximum velocity of EDTA 18% is 19 ms-1 and EDTA 18% wall shear stress is 1.56 KPa for calcium hydroxide removal observed from the CFD study. Keywords: Computational fluid dynamics, EDTA 18%, calcium hydroxide (Ca(OH)2), velocity, wall shear stress

Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis Azhar, Rodiyah; Julianti, Elin; Natasasmita, Setiawan; Dharsono, Hendra Dian Adhita
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods: Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.

Antibacterial potential of strawberries and basil extracts combination against Streptococcus sanguinis (ATCC 10556) Dharsono, Hendra Dian Adhita; Nurdin, Denny; Fatriadi, Fajar; Gondowidjojo, Yolanda Dwiutami; Yunita, Ellizabeth; Apriyanti, Eti
Padjadjaran Journal of Dentistry Vol 33, No 3 (2021): November
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no3.35894

Introduction: Streptococcus sanguinis is a commensal microorganism as well as a pioneer colony in forming dental plaque. Oral biofilm formation can be prevented by a mechanical cleaning procedure followed by the use of mouthwash. The current gold standard for mouthwash is chlorhexidine. Nevertheless, it has side effects that are not recommended for long-term use. Previous studies had proven that herbal-based mouthwashes such as basil leaves (Ocimum basilicum) and strawberry fruit (Fragaria x ananassa) have been shown to have antibacterial properties. The effectivity of antibacterial activity phenomenon in combined extracts has been reported in other studies. This research aims to observe the antibacterial potential of the F. x ananassa and O. basilicum extract combinations against S. sanguinis (ATCC 10556). Methods: The sample of this study was a combination of F. x ananassa and O. basilicum extract, which initially screened for their antibacterial activities. Antibacterial activities of F. x ananassa and O. basilicum extracts against S. sanguinis were observed using Kirby Bauer method, while Minimum Inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) by serial microdilution method. The 2% concentration from each extract was combined in 1:1, 1:2, and 2:1 volume ratio variations then tested for inhibitory zones, MIC, and MBC. Results: F. x ananassa extract had 0.125% and 0.25% for MIC and MBC respectively, while O. basilicum extract showed the value of MIC and MBC as 0.031% and 0.063% against S. sanguinis (ATCC 10556). The extract combinations in 1:1, 1:2, and 2:1 volume ratio variations showed 0.016% for MIC and 0.031% for MBC. Conclusions: It was concluded that combining extracts of 2 % F. x ananassa and 2% O. basilicum in various ratios were observably to have the antibacterial potential against S. sanguinis (ATCC 10556).

Antibacterial activity of strawberry fruit extract against Streptococcus sanguinis ATCC 10556) Nurdin, Denny; Dharsono, Hendra Dian Adhita; Fatriadi, Fajar; Sahara, Elvi; Adang, Rahmi Alma Farah; Gondowidjojo, Yolanda Dwiutami; Kurnia, Dikdik
Padjadjaran Journal of Dentistry Vol 34, No 1 (2022): March
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol34no1.37043

Introduction: Streptococcus sanguinis is a facultative anaerobic Gram-positive bacteria known as a pioneer that plays a role in creating the biofilm in the oral cavity. Strawberry fruit (Fragaria x ananassa) is an edible fruit widely used for the study as their active compound synergy to improve health. This study aims to analyse the antibacterial activity of strawberry fruit against S. sanguinis (ATCC 10556). Methods: An explorative study was conducted with high concentration methanol extract from strawberry fruit. Antibacterial activity was tested on the methanol extract; afterwards, the extract was fractionated and divided into three fractions: water, ethyl acetate, and hexane. Zone of inhibition was used to assess the most effective fraction among those three, then continued by testing for Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). Results: Inhibitory zone of the ethyl acetate fraction from strawberry fruit 1%, 2%, 3% ,4% and 5% sequentially were 7.3 mm,10.2 mm, 12.3 mm, 16.3 mm and 16.1 mm. Ethyl acetate fraction of 4% was the most effective to create the zone of inhibition with a size of 16.3 mm compared to the others. The Minimum Inhibitory Concentration (MIC) value was 0.25%. It was obtained by diluting a 4% ethyl acetate fraction on a microplate. The Minimum Bactericidal Concentration (MBC) was 2%. Conclusion: Ethyl acetate fraction was an effective fraction from strawberry fruit and had antibacterial activity against S. sanguinis with the inhibitory zone in the concentration of 4%, MIC of 0.25%, and MBC of 2%.

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