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All Journal Padjadjaran Journal of Dentistry JFIOnline Narra J Pharmaceutical Sciences and Research (PSR)
Julianti, Elin
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

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Isolasi dan Skrining Aktivitas Antimikroba Jamur Laut pada Alga Kappaphycus alvarezii dari Kabupaten Takalar Sulawesi selatan Fitriana, Fitriana; Julianti, Elin; Wibowo, Marlia Singgih
Jurnal Farmasi Indonesia Vol 8, No 2 (2016)
Publisher : Jurnal Farmasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1907.092 KB) | DOI: 10.35617/jfi.v8i2.521

Abstract

Marine microorganisms, especially actinomycetes bacteria and fungi produce a variety of secondary metabolites that biologically active and have a unique structure. This study aims to isolate some strains of marine fungi that grow on marine algae as a potential source of antimicrobial agent. Marine fungal strains isolated from the marine algae with antimicrobial activity screening is done by using the disc diffusion method. In this study, it was obtained 18 strains of fungal isolates. The screening results that give best antimicrobial activity shown by the activity of the extract liquid culture of fungal isolate strain AKT.C6 are 10.0 mm against the bacteria Escherichia coli and 10.8 mm against Bacillus subtilis. Mycelium extract of fungal isolates strain AKT.C4 is 16.9 mm against the bacteria Escherichia coli and 17.6 mm against Bacillus subtilis, while the liquid culture extract and mycelium extract did not show any activity against Candida albicans. The isolation of fungi from Kappaphycus alvarezii algaee has potential as an antibacterial agent.
Identification and Evaluation of Antibacterial Compounds from the Vibrio sp. associated with the Ascidian Pycnoclavella diminuta Dermawan, Abdurraafi’ Maududi; Julianti, Elin; Putra, Masteria Yunovilsa
Pharmaceutical Sciences and Research Vol. 6, No. 3
Publisher : UI Scholars Hub

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Abstract

This study aims to obtain secondary metabolites that have antibacterial activity from bacteria endophytic of ascidian Pycnoclavella diminuta collected from the coast of Bitung, North Sulawesi, Indonesia. The research was started with screening antibacterial activity of six bacteria isolated from P. diminuta. The active bacteria were selected for identification using standard biological molecular method and further fermentation to produce secondary metabolites. The isolation of secondary metabolites was conducted by various chromatography method and then selected fraction was based on the antibacterial activity from bioautography results. The result showed that the active isolate by coding M2.Tnk.Bt 5.10-3.2 was identified as Vibrio sp. The potential active fraction was further sub-fractionated by HPLC semi-preparative and each of these sub-fractions was tested against Staphylococcus aureus, Bacillus cereus, Enterococcus faecalis, Eschericia coli, and Vibrio cholerae. The test results showed that all 17 sub-fractions were active against Gram-positive bacteria S. aureus and B. cereus, and only one sub-fraction (SFr 2) showed antibacterial activity against E. faecalis. Whereas in Gram-negative bacteria, 2 sub-fractions (SFr 12 and SFr 13) showed antibacterial activity against E. coli and 12 sub-fractions active against V. cholerae. GC-MS analysis of the SFr 2 sub-fraction showed the presence of fifteen phytochemical constituents with a major compound Pyrrolo [1,2-a] pyrazine-1,4-dione, hexahydro-3- (2 methylpropyl).
Fungistatic activity and mechanism of Caulerpa racemosa, Caulerpa lentillifera fractions and caulerpin metabolite against pathogenic fungi Nursidika, Perdina; Julianti, Elin; Kurniati, Neng F.
Narra J Vol. 5 No. 1 (2025): April 2025
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v5i1.1714

Abstract

The increasing prevalence of azole resistance in various fungal species presents a significant concern, highlighting the urgent need for new antifungal agents. The aim of this study was to investigate the antifungal activity of fractions from Caulerpa racemosa, C. lentillifera, and caulerpin against three species: Aspergillus flavus, A. niger, and Candida albicans. The Caulerpa extracts were obtained through maceration with 96% ethanol, followed by fractionation using vacuum liquid chromatography. Antifungal activity was assessed using the broth microdilution method, while fungal growth kinetics were evaluated through time-kill curves. Bioautography was employed to identify inhibitory compounds, while liquid chromatography high-resolution mass spectrometry (LC-HRMS) was utilized to detect the contents of the extracts and fractions. Scanning electron microscopy (SEM) was used to observe the fungal structure, and the absorbance at 260/280 nm was measured to evaluate the cell leakage. LC-HRMS identified numerous compounds in C. racemosa and C. lentillifera with antifungal activities, including fatty acids, terpenes, alkaloids, flavonoids, and coumarins. The results indicate that the fractions of both Caulerpa did not inhibit the growth of A. flavus and A. niger, but effectively inhibited C. albicans. Among the fractions, F3CR and F4CL exhibited the highest antifungal efficacy against C. albicans, with minimum inhibitory concentrations (MICs) ranging from 64 to 128 µg/mL. Caulerpin, the primary metabolite of Caulerpa, also demonstrated significant inhibition, with an MIC of 256 µg/mL. The findings suggested that F3CR, F4CL, and caulerpin possessed fungistatic properties. Bioautography results revealed clear zones in the colonies, indicating inhibited fungal growth. The SEM observations showed that fungal cells became rough, perforated, and damaged, which was confirmed by the increase in absorbance at 260/280 nm, suggesting the release of cellular components such as nucleotides and proteins. In conclusion, both Caulerpa species and caulerpin are promising candidates for developing new antifungal agents against C. albicans.
Antibacterial activity of Zingiber officinale roscoe extract as a potential root canal irrigation solution against Enterococcus faecalis Azhar, Rodiyah; Julianti, Elin; Natasasmita, Setiawan; Dharsono, Hendra Dian Adhita
Padjadjaran Journal of Dentistry Vol 30, No 2 (2018): July 2018
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (589.594 KB) | DOI: 10.24198/pjd.vol30no2.18328

Abstract

Introduction: This study was aimed to determine and formulate antibacterial activity of Zingiber Officinale Roscoe (Z. officinale) extract against Enterococcus faecalis (E. faecalis) as a potential irrigating solution which is easily available and cost-effective. Methods:  Extract of Z. officinale Roscoe was yielded through soxhlet technique. Samples divided into two groups, i.e., the extract of Z. officinale Roscoe and chlorhexidine 2% as a control. The antimicrobial activity was observed using the diffuse agar method. Minimum inhibitory concentration (MIC) was determined by serial microdilution method, and minimum bactericidal concentration (MBC) was determined by cultured samples in blood agar. Data were analysed using ANOVA and Kruskal-Wallis test. Results: Extract of Zingiber officinale Roscoe has antimicrobial activity against E. faecalis with the MBC of 15.625 mg/mL. (p<0,05). Conclusion: Extract of Zingiber officinale Roscoe have shown antibacterial activity against E. faecalis and can be further developed as a potential root canal irrigation solution.
Co-Authors Azhar, Rodiyah Dermawan, Abdurraafi Maududi Hendra Dian Adhita Dharsono Kurniati, Neng F. MARLIA SINGGIH WIBOWO Perdina Nursidika Putra, Masteria Yunovilsa Setiawan Natasasmita Yoga Yuniadi