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AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Ame Suciati Setiawan; Fajar Fatriadi; Diani Prisinda
ODONTO : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.7.2.111-116

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

PENGALAMAN KARIES, KADAR HAEMOGLOBIN, BERAT BADAN DAN TINGGI BADAN PADA ANAK DENGAN LESI MUKOSA ORAL Indah Suasani Wahyuni; Fajar Fatriadi; Diani Prisinda; Fidya Meditia Putri; Nanan Nuraeny; Wahyu Hidayat
ODONTO : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.2.79-84

Background: Oral mucosal lesions in children caused by nutritional problems, dental infection, poor oral hygiene and trauma. Oral health in children as the next generation of the nation should always be maintained. However there is still lack of data on the emergence of oral mucosal lesions in Indonesian children. Purpose. To describe an overview of the caries experience, Haemoglobin/Hematokrit (Hb Ht), weight (BB) and height (TB) in children aged 6-7 years in several elementary schools at Tanjungsari, Sumedang.Result: 44 of 137 students who had oral mucosal lesions, had completesupporting data. Total of 43 students had an average caries experience of 8.4 teeth/child (1-17 teeth/child), while 1 student was caries-free. The average of weight = 19.3 kg, height = 115.5 cm, and Hb/Ht = 12.5/36.5%. Oral lesions found were plaques on the tongue, ulcers, macules, nodules, pustules and desquamatives.Discussion: The condition of untreated dental caries can cause pain anddiscomfort, so the child will have difculty eating and interfere with the growth process. This can be seen through the child’s caries experience with data on weight and height. Tooth decay aﬀects the nutrient intake and was estimated to lead to a decrease in Hb/Ht. Oral mucosal lesions due to trauma and poor oral hygiene were not expected to aﬀect systemic conditions.Conclusion: The results of this study indicate that the caries experience was very high, the average value of weight and height were normal below the ideal, Hb/Ht levels within normal limits, while Coated tongue was the common diagnosis found from subjects

Zona hambat ekstrak etil asetat daun kemangi (Ocimum basilicum) terhadap Enterococcus faecalis ATCC 29212Inhibition zone of ethyl acetate extract of basil leaves (Ocimum basilicum) against Enterococcus faecalis ATCC 29212 Rahmi Wastri; Ame Suciati Setiawan; Dani Rizali Firman; Diani Prisinda; Fajar Fatriadi
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 33, No 1 (2021): April 2021
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v33i1.28836

Pendahuluan: Enterococcus faecalis (E. faecalis) merupakan bakteri yang persisten pada saluran akar gigi, sehingga dapat menyebabkan infeksi sekunder pada saluran akar gigi yang telah diobturasi. Bahan irigasi dan medikamen harus diberikan pada saluran akar gigi untuk menunjang keberhasilan perawatannya. Daun kemangi (Ocimum basilicum/O. basilicum) merupakan tanaman obat tradisional yang diketahui memiliki potensi antibakteri dan berpeluang untuk dikembangkan sebagai bahan irigasi saluran akar gigi. Penelitian ini bertujuan untuk mengetahui zona hambat ekstrak etil asetat daun O. basilicum terhadap E. faecalis ATTC 29212. Metode:Jenis penelitian eksperimental laboratori. Pengujian zona hambat ekstrak etil asetat daun O. basilicum yang diperoleh dari proses maserasi dilakukan dengan metode difusi cakram dengan menggunakan klorheksidin 0,1% sebagai kontrol positif dan dilakukan tiga kali pengulangan terhadap E. faecalis ATCC 29212. Ekstrak pekat dilarutkan menggunakan DMSO 10% dan dilakukan pengenceran dua tingkat sehingga diperoleh konsentrasi ekstrak 512.000 – 1.000 ppm. Hasil: Ekstrak etil asetat daun O. basilicum pada konsentrasi uji 512.000 ppm telah memperlihatkan zona abu-abu seluas 1 mm di luar cakram uji, sementara konsentrasi uji lainnya tidak memperlihatkan adanya penghambatan. Beragam faktor dalam penelitian ini dapat mempengaruhi hasil pengujian, diantaranya adalah kelarutan ekstrak uji dalam pelarut yang digunakan, prosedur sebelum ekstraksi, dan keterbatasan metoda pengujian. Simpulan: Ekstrak etil asetat daun O. basilicum tidak memiliki zona hambat terhadap E. faecalis ATTC 29212.Kata kunci: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zona hambat. ABSTRACTIntroduction: Enterococcus faecalis (E. faecalis) is a bacteria that persist in root canals, cause secondary infection in obturated root canals. Irrigation material and medicament must be administered to the root canal to support the treatment success. Basil leaves (Ocimum basilicum/O. basilicum) is a traditional medicinal plant known with antibacterial potential and has the opportunity to be developed as a root canal irrigation agent. This study was aimed to determine the inhibition zone of the ethyl acetate extract of O. basilicum leaves against E. faecalis ATTC 29212. Methods: This study was an experimental laboratory. The inhibition zone test of the ethyl acetate extract of O. basilicum leaves obtained from the maceration process was conducted with the disc diffusion method using 0.1% chlorhexidine as a positive control and three replications E. faecalis ATCC 29212. The concentrated extract was dissolved using 10% DMSO, and two dilution levels were carried out to obtain an extract concentration of 512,000 - 1,000 ppm. Results: The ethyl acetate extract of O. basilicum leaves at the test concentration of 512,000 ppm showed a grey zone of 1 mm outside the test disc, while other test concentrations did not show any inhibition. Various factors in this study can affect the test results, including the solubility of the test extract in the solvent used, the procedure before extraction, and the limitations of the test method. Conclusion: O. basilicum leaf ethyl acetate extract had no inhibition zone against E. faecalis ATTC 29212.Keywords: Ocimum basilicum, Enterococcus faecalis ATCC 29212, zone of inhibition.

AKTIVITAS ANTIBAKTERI FRAKSI ETANOL DAUN KEMANGI (Ocimum americanum) TERHADAP Enterococcus faecalis ATCC 29212 Ame Suciati Setiawan; Fajar Fatriadi; Diani Prisinda
Odonto : Dental Journal Vol 7, No 2 (2020): December 2020
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Ocimum americanum (O. americanum) is a natural plant with antibacterial effects inhibits the growth of gram-positive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis (E. faecalis) is a gram-positive anaerobic bacteria in the root canal which is resistance to intracanal medicament. The purpose of this study is to analyze the antibacterial effect of ethanol fractionation of O. americanum towards E. faecalis ATCC 29212. Ocimum americanum is a natural plant that has antibacterial properties against grampositive and gram-negative anaerobic bacteria in the oral cavity. Enterococcus faecalis is an anaerobic gram-negative bacteria was found in root canals and resistant to various root canal medicament. The purpose of this study was to analyze the antibacterial effect of the ethanol fraction of Ocimum americanum against Enterococcus faecalis. Methods: The research method was experimental used disc diffusion technique. The fraction is made from solid extract into a polar solution with ethanol solvent with concentration 20%, 40%, 60% and 80%. The test was performed by measuring the inhibition zones around the disc after 24 hours.Results: Chemical properties of O.americanum are alkaloid, flavonoid, saponin, tanin, steroid, dan terpenoid. Inhibition zone of O. americanum fraction with concentration 20%, 40%, 60% and 80% is 8.8 cm, 9.8 cm, 11.1 cm and 16.7 cm. The measurement of O. americanum inhibition zone showed better results compared than positive control (Minosep).Conclusion: Ethanol fraction of O.americanum has antibacterial effect towards E. faecalis.

PENGALAMAN KARIES, KADAR HAEMOGLOBIN, BERAT BADAN DAN TINGGI BADAN PADA ANAK DENGAN LESI MUKOSA ORAL Indah Suasani Wahyuni; Fajar Fatriadi; Diani Prisinda; Fidya Meditia Putri; Nanan Nuraeny; Wahyu Hidayat
Odonto : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Background: Oral mucosal lesions in children caused by nutritional problems, dental infection, poor oral hygiene and trauma. Oral health in children as the next generation of the nation should always be maintained. However there is still lack of data on the emergence of oral mucosal lesions in Indonesian children. Purpose. To describe an overview of the caries experience, Haemoglobin/Hematokrit (Hb Ht), weight (BB) and height (TB) in children aged 6-7 years in several elementary schools at Tanjungsari, Sumedang.Result: 44 of 137 students who had oral mucosal lesions, had completesupporting data. Total of 43 students had an average caries experience of 8.4 teeth/child (1-17 teeth/child), while 1 student was caries-free. The average of weight = 19.3 kg, height = 115.5 cm, and Hb/Ht = 12.5/36.5%. Oral lesions found were plaques on the tongue, ulcers, macules, nodules, pustules and desquamatives.Discussion: The condition of untreated dental caries can cause pain anddiscomfort, so the child will have difculty eating and interfere with the growth process. This can be seen through the child’s caries experience with data on weight and height. Tooth decay aﬀects the nutrient intake and was estimated to lead to a decrease in Hb/Ht. Oral mucosal lesions due to trauma and poor oral hygiene were not expected to aﬀect systemic conditions.Conclusion: The results of this study indicate that the caries experience was very high, the average value of weight and height were normal below the ideal, Hb/Ht levels within normal limits, while Coated tongue was the common diagnosis found from subjects

Perbedaan densitas email normal dengan email yang diremineralisasi secara in vitro menggunakan sediaan NaF, CPP-ACP, dan karbonat apatit: studi eksperimental Paquita, Elissa; Hidayat, Opik Taofik; Fatriadi, Fajar; Lita, Yurika Ambar
Padjadjaran Journal of Dental Researchers and Students Vol 7, No 2 (2023): Juni 2023
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjdrs.v7i2.44293

ABSTRAKPendahuluan: Penurunan densitas gigi terjadi ketika komponen mineral dalam gigi mengalami demineralisasi akibat asam organik yang menempel pada permukaan gigi. Hal ini dapat menyebabkan terjadinya karies gigi yang sampai sekarang masih menjadi masalah kesehatan gigi di sebagian besar Negara. Penelitian ini bertujuan menganalisis perbedaan densitas jaringan email normal dengan hasil tindakan remineralisasi secara in vitro menggunakan sediaan NaF, CPP-ACP, dan karbonat apatit pada sampel gigi premolar rahang atas yang telah didemineralisasi buatan. Metode: Jenis penelitian eksperimental murni menggunakan tiga puluh gigi premolar rahang atas yang dipotong di CEJ untuk menyisakan bagian mahkota. Sampel ditanam tegak lurus pada kubus akrilik berukuran 2x2 cm. Demineralisasi buatan dilakukan dengan cara merendam sampel di larutan asam sitrat 1% selama 24 jam. Sampel kemudian dibagi secara acak menjadi tiga kelompok dengan masing-masing berisi 10 sampel yang akan dioles menggunakan NaF, CPP-ACP, dan karbonat apatit dan dibiarkan selama 4 menit. Tindakan remineralisasi diulang selama 28 hari. Radiografi periapikal digunakan untuk melihat densitas mineral dengan melakukan foto radiografi sampel sebelum demineralisasi, setelah demineralisasi, dan setelah remineralisasi. Hasil foto akan dianalisis densitasnya menggunakan software ImageJ. Hasil: Nilai Rerata densitas email setelah remineralisasi adalah sebesar 10,955%, 12,369%, dan 11.297% untuk bahan NaF, CPP-ACP, dan karbonat apatit secara berurutan. Analisis data dengan uji one-way ANOVA menunjukkan perbedaan yang signifikan (p=0.043). Simpulan: Aplikasi topikal NaF, CPP-ACP, dan karbonat apatit dapat secara signifikan meningkatkan densitas email yang terdemineralisasi melalui proses remineralisasi, dan yang paling baik diantaranya adalah CPP-ACP.KATA KUNCI: remineralisasi, densitas email, NaF, CPP-ACP, karbonat apatit.The difference in density between normal enamel and enamel after in-vitro remineralization using readymade NaF, CPP-ACP, and apatite carbonate: Experimental studyABSTRACTIntroduction: Decrease in tooth density occurs when the mineral components in the teeth experience demineralization due to organic acids that adhere onto the tooth surface. This can lead to dental caries which is still a dental health problem in most countries. This study aims to determine the difference in density between normal enamel and enamel after in-vitro remineralization using readymade NaF, CPP-ACP, and apatite carbonate in maxillary premolar samples that have been artificially demineralized. Methods: The method of this study was pure experimental using thirty maxillary premolars which were cut at the CEJ to leave part of the crown. Samples were planted perpendicularly on 2x2 acrylic cubes. Artificial demineralization was done by immersing samples in 1% citric acid solution for 24 hours. They were then divided randomly into three groups that consists 10 samples each which would be smeared with NaF, CPP-ACP and carbonate apatite and left for 4 minutes. The remineralization action was repeated for 28 days. Periapical radiography was used to see mineral density by performing radiographic photographs of samples before demineralization, after demineralization, and after remineralization. The results will be analyzed for density using ImageJ software. Results: The average values of enamel density after remineralization were respectively 10.955%, 12.369% and 11.297% for NaF, CPP-ACP and carbonate apatite. Data analysis using one-way ANOVA test showed a significant difference (p=0.043). Conclusion: Topical application of NaF, CPP-ACP and carbonate apatite can significantly increase the density of demineralized enamel through remineralization process, with the best being CPP-ACP.KEY WORDS: remineralization, enamel density, NaF, CPP-ACP, carbonate apatite.

ENDODONTIC TREATMENT ON FRACTURED MANDIBULAR ANTERIOR TOOTH WITH CROWN DOWN TECHNIQUE Putri Lesmana, Raisya Aurellia; Fatriadi, Fajar
Dentino: Jurnal Kedokteran Gigi Vol 9, No 1 (2024)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v9i1.18872

Background: Trauma involving the dento-alveolar area often occurs and tends to cause tooth fractures. Fractured teeth can be at risk of infection which can lead to pain and tooth necrosis. In necrotic tooth cases, root canal treatment is one of the most common procedures to be performed to preserve the tooth. Case: A 36-year-old female patient came to RSGM Unpad with chief complaint of a broken mandibular anterior tooth. This complaint occurred 3 months ago. The patient did not complain of any pain. Previously, the tooth that the patient complained about had been restored but fell out again a month ago. History of systemic disease, family disease, and allergies was denied. Patients want their complaints to be resolved. Case Management: The treatment carried out in this case is root canal treatment. Root canal treatment consists of three main stages, namely biomechanical preparation of the root canal, root sterilization with calcium hydroxide, and obturation. The preparation technique used is crown down. The main aim of the crown down root canal preparation technique is to minimize or eliminate the amount of extruded necrotic tissue towards the apical foramen during preparation. Conclusion: Establishing a good diagnosis, as well as the use of good instrumentation in root canal treatment with a crown down preparation technique followed by single cone obturation can be an option for treating crown fracture teeth. Further follow-up is required in this case. Keywords: crown down, fracture, nonvital, root canal treatment

INDIRECT PULP CAPPING TREATMENT WITH CLASS I COMPOSITE RESTORATION FOLLOW-UP IN SECOND RIGHT MANDIBULLARY MOLAR Gracesheila, Thanya; Fatriadi, Fajar
Media Penelitian dan Pengembangan Kesehatan Vol. 33 No. 3 (2023): MEDIA PENELITIAN DAN PENGEMBANGAN KESEHATAN
Publisher : Poltekkes Kemenkes Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34011/jmp2k.v33i3.1667

Pulp capping adalah perawatan proteksi kompleks dentin-pulpa yang dilakukan dengan mengaplikasikan material spesifik diantara bahan restorasi dan dentin untuk mencegah terbukanya pulpa secara iatroganik, toksisitas material restorasi, dan penetrasi bakteri. Perawatan indirect pulp capping dapat mempertahankan vitalitas pulpa dengan memfasilitasi terjadinya penyembuhan dan perbaikan jaringan pulpa dengan minginduksi terbentuknya dentin reparatif. Penelitian ini mengeksplorasi keberhasilan penanganan pulpitis reversibel melalui perawatan indirect pulp capping menggunakan Kalsium Hidroksida. Pasien laki-laki berusia 22 tahun memiliki keluhan nyeri tajam dan intermiten pada gigi molar kanan mandibula sejak 3 bulan yang lalu, tanpa riwayat nyeri spontan. Temuan klinis menunjukkan adanya lesi karies dalam yang mencapai dentin dan meninggalkan lapisan tipis dentin pada gigi 47. Uji vitalitas pulpa menunjukkan pulpa vital. Diagnosis yang ditegakkan adalah pulpitis reversibel dengan jaringan apikal normal. Perawatan indirect pulp capping dengan restorasi komposit kelas I lanjutan dilakukan dalam 3 kali kunjungan dan pada kunjungan ketiga pemeriksaan subjektif dan objektif menunjukkan hasil yang baik dan pasien merasa puas dengan perawatan yang diberikan. Laporan kasus ini menggarisbawahi keberhasilan perawatan indirect pulp capping dengan Kalsium Hidroksida dalam menangani pulpitis reversibel, meskipun demikian, penelitian di masa depan dan tindak lanjut yang lebih lama sangat disarankan.

Velocity and wall shear stress of 18% EDTA irrigation solution flow in the removal of Ca(OH)2 with computational fluid dynamic analysis Muryani, Anna; Dharsono, Hendra Dian Adhita; Zuleika, Zuleika; Moelyadi, Mochammad Agoes; Fatriadi, Fajar
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Ethylene Diamine Tetra Acetic Acid (EDTA) 18% irrigation solution is one of the chelating agents that able to remove calcium hydroxide (Ca(OH)2) from the root canal wall. Failure of root canal treatment can be caused by the presence of residual calcium hydroxide in the root canal wall, thus blocking the bond between the medication and root canal filling material with the root canal wall. This study was aimed to observe the EDTA 18% flow in removing the Ca(OH)2 using CFD analysis. Methods: This research was descriptive. Cleanliness of the root canal wall from Ca(OH)2 medicament using EDTA 18% irrigation was analysed using the CFD method with test specimens in the form of resin blocks made according to the characteristics of the root canal. The irrigation needle used was side-vented with a position of 3 mm from the apical tooth. Stage analysis of root canal geometry was performed using Computational Fluid Dynamics (CFD) analysis to observe the characteristics of irrigation solutions in root canals in 3D. Results: The streamlined characteristics of EDTA 18% irrigation solution showed a unique behaviour due to the features of the side-vent shaped irrigation needle. Irrigation flow in the crown area of the inlet (side-vented irrigation needle) showed low velocity so that the fluid flow when exiting the inlet was more towards the apical than the outlet (root canal orifice). Conclusion: Velocity and wall shear stress of EDTA 18% showed the results validation conformity between experimental and CFD, that the maximum velocity of EDTA 18% is 19 ms-1 and EDTA 18% wall shear stress is 1.56 KPa for calcium hydroxide removal observed from the CFD study. Keywords: Computational fluid dynamics, EDTA 18%, calcium hydroxide (Ca(OH)2), velocity, wall shear stress

Antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers towards Streptococcus sanguis ATCC 10566 Fatriadi, Fajar; Kurnia, Dikdik; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 30, No 3 (2018): November 2018
Publisher : Universitas Padjadjaran

Introduction: Streptococcus sanguis is an initial cause of dental plaque formation which is the initial cause of caries. One of the preventive treatments can be done by using the mouthwash containing antibacterial substances. Along time, natural remedies are proven to be having more antibacterial properties. Ant-plant (Myrmecodia pendens Merr. & Perry) tubers are types of epiphytic plant that grows in many parts of Papua, with many health benefits, and are known to contain flavonoids, tannins, and tocopherols. This study was aimed to determine the antibacterial activity of ethyl acetate fraction from methanolic extracts of ant-plant tubers on the growth of Streptococcus sanguis ATCC 10566. Methods: The ant-plant tubers were extracted with soxhletation method using the methanol solvent. The fractionation was then performed using ethyl acetate to obtain the ethyl acetate fraction. Result: The phytochemical test showed that the ethyl acetate fraction of the ant-plant contained phenolics, tannins, flavonoids, and terpenoids. The bacterial test in this study was using the microdilution method using the ELISA Reader by measuring the Minimum Inhibitory Concentration (MIC) of ethyl acetate fraction of ant-plant tubers towards Streptococcus sanguis with positive control was using the chlorhexidine gluconate as the golden standard medication. The ethyl acetate fraction of ant-plant tuber had an antibacterial effect towards Streptococcus sanguis in the MIC of 31.25 ppm, while chlorhexidine gluconate was 0.49 ppm. Conclusion: The ethyl acetate fraction of ant-plant had a lower antibacterial activity compared to chlorhexidine gluconate on the growth of Streptococcus sanguis ATCC 10566.Keywords: Ant-plant tubers, Myrmecodia pendens Merr. & Perry, antibacterial activity, Streptococcus sanguis ATCC 10566.

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